Gamal Thabet

National Cancer Institute Egypt | NCI · Clinical pathology

Objectives: This study aims to investigate hemostatic changes in patients with coronavirus disease (COVID-19) and their relationship to disease severity and survival. Methods: This study included 284 patients with COVID-19 who attended the Security Forces Hospital, Makkah, Saudi Arabia between October 2020 and March 2021, and retrospectively review...

This study was designed to evaluate the effect of Methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms on MTX toxicity in pediatric Egyptian ALL patients. Ninety-Four of Pediatric ALL patients aged 3-13 years (7.6 ± 3.6) on oral maintenance dose of 50 mg/m2 weekly of MTX. MTHFR c.677C>T (rs1801133) and c.1298A>C (rs1801131) genotyping wer...

Background Type1 diabetes mellitus (T1DM) has a multi-factorial pathogenesis; the interplay between genetic susceptibility and environmental factors is thought to provide the fundamental element for the disease. Apart from HLA, more than 50 genetic variants are associated with T1DM. INS -23/Hph1 A>T (rs689) is one of the effective loci with inconsi...

Background: Prostate cancer (PC) is considered the fifth most common cancer causing death worldwide. Many studies have pointed to dysregulated microRNA (miRNA) expression in PC and their use in early detection and follow-up of the disease. In addition, the Prostate Health Index (PHI) is the FDA-approved blood test joining total, free, and -2proPSA...

Background: Genotypic mutation of FLT3, NPM1, and DNMT3A has been involved in the leukemogenesis of acute myeloid leukemia, with well-known poor prognostic role of FLT3 and DNMT3A and favourable for NPM1 mutation. Methods: A total of 123 patients with AML treated at NCI, Cairo University were examined for mutations in DNMT3A, FLT3 and NPM1 by poly...

Graft-versus-host disease (GVHD) is the major complication of allogeneic hematopoietic stem cell transplantation (HSCT); cytokines are recognized as important mediators in its pathogenesis. In this study we investigated the role of cytokine gene polymorphisms on HSCT outcome. A total of 106 patient and 98 donors were genotyped by polymerase chain r...

To study the effect of MATE 1, MATE 2 and OCT1 genetic variants on metformin action in recently diagnosed Egyptian Type-2 diabetic patients. Patients & Methods: One hundred type-2 DM patients and forty healthy control were included in the study. All patients were recently diagnosed receiving no treatment before participation in the study. Three sin...

Background and objectives: Detection of chromosomal abnormalities in myeloproliferative disorders is important for proper diagnosis of these disorders. This study has investigated the presence of JAK2 mutation (V617F) in Egyptian patients with myeloproliferative disorders referred to National Cancer institute, Cairo University. Methods: The stud...

Background and Purpose: The etiology of pediatric Acute lymphoblastic leukima is multifactorial. Several studies reported that genetic polymorphisms are associated with an altered risk of development of ALL. 5,10-Methylenetetrahydofolate reductase (MTHFR), Glutathione S-Transferase gene (GSTM1, GSTT1, GSTP1), NADP(H): quinine oxidoreductase (NQO1),...

N-Acetyltransferases (NAT) have been known to modify the risk to a variety of solid tumors. However, the role of NAT2 polymorphism in risk susceptibility to childhood acute lymphoblastic leukemia (ALL) is still not well known. We performed a case-control study to determine if the common NAT2 polymorphisms play a role in altering susceptibility to p...

In spite of prophylactic measures GVHD continues to be one of the serious complications that affect transplantation-related morbidity and mortality. We have previously reported a role of cytokines in the prediction of aGVHD (Kamel et al, 54th ASH Annual meeting, December 8-11.2012.Publicaion Number 1956. Nov 2012; 120: 195 and J Leuk 2013, 1:2 http...

Introduction: P-glycoprotein (PGP), encoded by the MDR1 gene is a trans-membrane active efflux pump for a variety of environmental toxins and xenobiotics. Studies have evaluated the association between MDR1 C3435T polymorphism, cancer susceptibility and therapeutic outcome. Methods: In our study we identified the MDR1 C3435T polymorphism in 94 bre...

In spite of prophylactic measures GVHD continues to be one of the serious complications that affect transplantation-related morbidity and mortality. We have previously reported a role of cytokines in the prediction of aGVHD (Kamel et al, 54th ASH Annual meeting, December 8-11.2012.Publicaion Number 1956. Nov 2012; 120: 195 and J Leuk 2013, 1:2 http...

Cytochrome P450 3A4 (CYP3A4) is the most plentiful cytochrome P450 in adult human liver and small intestine and is responsible for detoxification of more than 50% of drugs in addition to the metabolic deactivation and metabolism of many carcinogens. Polymorphism of CYP3A4-A-290G considered the only allele that appears to stimulate CYP3A4 expression...

Background: Type 1 diabetes is one of the most common chronic childhood illnesses. Interplay between genetic susceptibility and environmental factors is thought to provide the fundamental element for the disease. Apart from the Major Histocompatibility locus which is the main contributor to risk susceptibility, more than 40 loci are recognized. One...

Background: The P53 tumor suppressor gene plays a pivotal role in maintaining cellular homeostasis by preventing the propagation of genome mutations. P53 in its transcriptionally active form is capable of activating distinct target genes that contribute to either apoptosis or growth arrest, like P21. However, the MDM2 gene is a major negative regu...

Abstract: Molecular characterization of acute myeloid leukemia (AML) allows prognostic stratification and assessment of the chances of durable treatment response. The presence of FLT3 internal tandem duplication (ITD) mutation as well as the allelic ratio (ITD-AR) and JAK2 V617F mutation may be associated with clinical outcome in patients with AML....

Abstract: Background: Mercaptopurine is one of the most important drugs used in cancer treatment. Its elimination depends mainly on the enzyme Thiopurine S-methyl Transferase (TPMT). A number of known genetic polymorphisms can affect the activity of this enzyme. Aim of the work: to study the pattern of TPMT polymorphisms in a cohort of Egyptian pat...

Acute lymphoblastic leukemia is the major pediatric cancer in developed countries. To date most association studies of acute lymphoblastic leukemia have been based on the candidate gene approach and have evaluated a restricted number of polymorphisms. Such studies have served to highlight difficulties in conducting statistically and methodologicall...

ALL is the most common pediatric cancer. The causes of the majority of pediatric acute leukemia are unknown and are likely to involve an interaction between genetic and environmental factors. Therefore, unfavourable gene-environmental interactions might be involved in the genesis of ALL. The aim of this work was to evaluate, in a case-control study...

Background and Aim: Diagnosis of meningeal local- ization of lymphoid malignancies by means of cytologic examination of the cerebrospinal fluid (CSF) can be difficult. Thus far, no reliable CSF tumor marker has been identified. The aim of this study is to determine prospectively the value of CSF soluble interleukin-2 receptor (sIL-2R) for the diagn...