Gamal Abd El Raheem Sosa

Benha University · Department of Theriogenology

The scenario of the fertile spermatozoa with high fertilizing capability is basically dependent on gene expression‐based epididymal function. The current investigation aimed to declare the varied expression of different candidate genes ( PLA2G4D , LCN15 , CLUAP1 , SPP1 , AQP12B , DEFB110 and ESR1 ) relevant to spermatozoa features between the diffe...

Influence of IBR vaccination timing on ovarian and uterine statuses and circulating progesterone and estradiol 17β in synchronized crossbreed heifers

Studying testicular genes´ expression may give key insights into precise regulation of its functions that influence epididymal sperm quality. The current study aimed to investigate the abundance of candidate genes involved in the regulation of testicular functions specially those regulate sperm function (PLA2G4D, SPP1 and CLUAP1), testicular steroi...

The study aimed to declare the influence of the epididymal region on spermatozoa characteristics and oviduct reservoir potential in dromedary camel. Testicles with epididymis of mature dromedary camels aged 5-10 years old (n= 27) were collected during the rutting season (December – April). Spermatozoa collected from the three main anatomical epidid...

This study aimed to assess the relation between the morphometric and histological attributes of the epididymis, and epididymal semen features in mature dromedary camels. The testes with the attached epididymis (n=50) were collected from adult camels along the rutting season from December to April. The separated epididymis was evaluated for weight a...

This study aimed to assess the relation between the morphometric and histological attributes of the epididymis, and epididymal semen features in mature dromedary camels. The testes with the attached epididymis (n=50) were collected from adult camels along the rutting season from December to April. The separated epididymis was evaluated for weight a...

The present study aimed to evaluate the effect of brilliant cresyl blue (BCB) stain on quality and developmental competence of mature and immature buffalo oocytes. Oocytes were exposed to BCB stain diluted in mDPBS (DPBS with 0.4% BSA) for 60 min at 38.5 º C in a humidified air atmosphere before, after maturation. The cleavage and blastocyst rates...

Objective: To assess the effects of melatonin and/or cysteamineon in vitro maturation, culturing and post-warming of buffalo embryos. Methods: Buffalo oocytes were classified into control, cysteamine (50 μM), melatonin (10 ng/mL) and cysteamine (50 μM) + melatonin (10 ng/mL) treatment groups. In experiment 1, previous treatments were added during i...

Objective: To improve in vitro embryo production in buffalo by supplementation of L-ascorbic acid during maturation and development (experiment 1) and combination with another antioxidant as cysteamine (experiment 2). Methods: Two experiments were performed, the first one aimed to evaluate the different concentrations (0, 25, 50, 100 µM) of L-ascor...

The present investigation aimed to outline the effects of PNP on Amphiregulin (AREG)-induced oocytes maturation. Bovine COCs were aspirated from small antral follicles (2–6 mm in diameter) and incubated in IVM medium contained 1 µl /ml Amphiregulin of human origin supplemented with PNP at 0 (control), 10 7 M (LD), 10 M (MD), 10 M (HD) and 10 M (VHD...

The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvest...

OR IMPROVING embryo development in buffalo, two experiments were conducted. The first one was carried out to evaluate the different concentrations (0, 5, 25, 50 ng/ml) of epidermal growth factors (EGF) on developmental competence of buffalo oocytes. The selected oocytes were cultured in the four concentrations of EGF. The embryo cleavage rate was s...

Acute Renal Failure (ARF) has traditionally been defined as the abrupt and progressive loss of kidney functions resulting in the retention of urea and other nitrogenous waste products associated with interstitial inflammation, tubular injury and increasing Tumor Necrosis Factor (TNF). Mortality in patients with ARF remains high >50% in severely ill...

The aim of this work was to evaluate the effect of relaxin on fertility parameters of buffalo frozen/thawed sperm. Sperm were incubated in the absence of capacitating agents (negative control), with a known capacitating agent such as heparin (positive control) and with 50 and 100 ng/ml relaxin for 2 and 4 h. Sperm viability, motility, capacitation...

The objective of this study was to evaluate efficacy of diagnostic techniques of endometritis in Arabian mares. An ultrasonographic evaluation as well as cytological and bacteriological examinations of uterine swabs have been performed in Arabian mares (n=43) suffered from endometritis. Mares showed a highly significant (P<0.001) impact of age on t...

The ovine testis undergoes several histological changes during it's development, there are three stages in development, prepuberty stage, maturity stage and adulthood stage. At early ages from the first month till the fifth months (prepuberty stage), no spermatogenesis is initiated and the testicular parenchyma is composed of spermatic cords only w...

The aim of this study was to determine the effect of Methyl-B-Cyclodextrin (MBCD) on capacitation of buffalo sperm. Frozen/thawed semen was incubated in the absence of capacitating agents (negative control), in the presence of 10 µg/ml heparin (positive control) and of 1, 2, 4 and 8 mg/ml MBCD for 2 and 4h. At each incubation time, sperm motility w...

This study aimed to determine the most reliable method to evaluate capacitation of buffalo frozen/thawed sperm. Frozen/thawed sperm were incubated in TALP medium in absence of capacitating agents (control) and in presence of 10 μg/ml heparin for 2 and 4 hrs. Capacitation was assessed by Trypan blue/Giemsa after lysophosphatidylecholine (LPC) exposu...

A B S T R A C T CYP19 is a member of cytochrome P450 aromatase family, which regulates estrogen biosynthesis in animals so it can be used as a candidate gene for marker-assisted selection strategies for fertility in buffaloes. The sequence of this gene in buffalo was recently published but association study has not conducted yet. Therefore, the obj...

Numerous studies have been investigated to assess the relationship between the fertility related gene, CYP19, and reproduction in cow; however only scanty studies were performed on CYP19 gene in buffalo. The present study was designed to isolate a fragment of CYP19 gene containing exon 9 and to detect any polymorphism associated with infertility in...

The ovine epididymis undergoes several histological changes during its development, these changes are occurred in type of lining epithelium which begins with ciliated low columnar then with age changed to ciliated pseudostratified columnar epithelium, this differentiation is initiated firstly in tail then body and head regions. There are three stag...

Vitrification is a common method for cryopreservation of gametes and embryos. Although successful oocyte vitrification has been achieved in several animal species, subsequent progress is still limited especially in buffalo. To improve the effectiveness of vitrification of buffalo oocytes, two experiments were conducted. The first experiment evaluat...

The aim of this work was to evaluate the effect of relaxin, known to improve fertility parameters of frozen-thawed sperm in other species (Miah et al. 2006 J. Reprod. Dev. 52, 773-779; Miah et al. 2007 Anim. Sci. J. 78, 495-502), on buffalo sperm motility, capacitation, and fertilizing capability. Frozen-thawed sperm from 2 bulls (4 replicates each...

A B S T R A C T This study was carried out to evaluate the genetic diversity and phylogenetic relationships using randomly amplified polymorphic DNA (RAPD) in four chicken populations; Fayoumi × Fayoumi (FF), Fayoumi × Rhode Island Red (FR), Rhode Island Red× Fayoumi (RF) and Rhode Island Red× Rhode Island Red (RR). Fifteen decamer (RAPD) primers w...

The present study aimed at instigating the buffalo ovarian and uterine response to ovsynch protocol when initiated 21 days postpartum (pp). Animals were arranged into a treated (n = 3) and a control (n = 3) group. The treated group was given the first dose of GnRH on day 0 (day 21 pp) followed by PGF2 alpha on day 7 and the second dose of GnRH on d...

The intensity of heat signs in buffaloes is generally low and the incidence of suboestrus varied from 15 to 73% (Buffalopedia). The objective of this study was to investigate the feasibility of monitoring the changes in some milk constituents, oestradiol levels and electrical conductivity of vaginal mucus during peri-oestrous period in prediction o...

Vitrification is a common method which is successfully used for cryopreservation of gametes and embryosin in several animal species, though subsequent progress is still limited,especially in buffalo. The present study aimed to evaluate the effect of vitrification process on nuclear status in matured buffalo oocytes. Morphology, viability, state of...

This study was carried-out to throw the light on the most important factors affecting economic and productive efficiency of beef production in Egypt through field survey in Kaliobyia, Giza and Monofyia provinces during the period extended from winter 2010 to summer 2012 on random samples of beef production sectors. These sectors were Farmers (Falla...

This study was conducted to evaluate body weights, average daily gain (ADG), relative growth rate (RGR), average egg weight, hen day egg production% (HDEP%), hen housed egg production% (HHEP%), age at sexual maturity, body weight at sexual maturity, fertility%, scientific and commercial hatchability percentage in a complete 2x2 diallel crossbreedin...

To evaluate the comparative efficacy of Ovsynch and Heatsynch protocols with respect to ovarian response, estrus, pregnancy rate and progesterone concentrations, 29 cyclic buffalo heifers were assigned randomly to one of four treatments: GPG treatment (n= 8) received two GnRH doses, one seven days before and the other two days after a single dose o...

The current study was designed to find out the effect of different doses (8, 12 or 16 µg) of GnRH analogue (Buserelin acetate, BA) on the ultrasonographic picture and biometry of buffalo bulls' reproductive organs. Animals (n= 9, aged 15-18 months) were allocated into three groups (n= 3/group) according to the hormonal dose that was administered on...

To clarify the effect of lactation period on ovarian follicular activity and associated hormonal levels in goats, six goats were monitored daily by ultrasonographic examination with blood sampling during early (Days 5 to 25; Day 0 was the day of kidding) and late (Days 40 to 60) lactation. While the presence of a corpus luteum of pregnancy retarded...

Melatonin is a multifunctional molecule that mediates several circadian and seasonal reproductive processes. The exact role of melatonin in modulating reproduction, however, is not fully understood-especially its effects on the ovarian follicles and oocytes. This study was conducted to investigate the expressions of the ASMT and melatonin-receptor...

The present study was conducted to characterize follicular development and its hormonal control during early pregnancy in goats. The ovaries of goats (n=8) were scanned daily for follicles (> or = 2 mm in diameter) and corpora lutea by transrectal ultrasound with blood sampling from the jugular vein for monitoring the hormonal changes during the fi...

The current study was performed to follow up the circulating hormonal changes and to correlate the findings with the physiological activity of the corpus luteum (CL) and placenta during pregnancy in goats. Blood samples were collected weekly from five goats during pregnancy for measuring steroid and protein hormones. A gradual increase was observed...

One of the primary shortcomings of adobe, a dried mudblock stabilized with sisal hemp and cement widely used across all of Latin America, is its dimensional instability when exposed to water or aggressive environments. The present paper evaluates the electrochemical performance of reinforced adobe block after 1,000 days of exposure to a simulated m...

One of the primary shortcomings of adobe, a dried mud block stabilized with sisal hemp and cement widely used across all of Latin America, is its dimensional instability when exposed to water or aggressive environments. The present paper evaluates the electrochemical performance of reinforced adobe block after 1,000 days of exposure to a simulated...

Selected buffalo oocytes were matured in vitro on TCM-199 and incubated for either 16, 18. 22, 24 or 30 hours (Experiment, 1); on either TCM-199, Ham's F-10 or MEM media and incubated to 24 hours (Experiment, 2): TCM-199 supplemented with 10% of either FCS. EBS. DBS or PBS (Experiment, 3). Rates of maturation, fertilization, cleavage and embryo dev...

Ovaries from adult buffalo cows of unknown breeding history were collected from a slaughter House. They were transported into the IVF laboratory within 2 to 4 hours post-slaughter. Oocytes were left in the post mortum ovaries for 2,3,4,6 and 8 hours post-slaughter before oocyte collection (Experiment, 1). According to their morphology, ovaries had...

Ovaries from adult buffalo cows of unknown breeding history were collected from a slaughter House. They were transported into the IVF laboratory within 2 to 4 hours post-slaughter. Oocytes were left in the post mortum ovaries for 2,3,4,6 and 8 hours post-slaughter before oocyte collection (Experiment, 1). According to their morphology, ovaries had...

The matured oocytes were incubated in F-TALP medium and inseminated by using either fresh or frozen-thawed semen at a concentration of 2 x 10 6 sperm cell/ml (Experiment,1). Other matured oocytes incubated in F-TALP medium were inseminated by using fresh semen at sperm concentration of either 0.5, 1.0, 2.0, 3.0. 9.0 or 10 x l0 6 sperm cells/ml (Exp...