Galina Vitalievna Reva

Far Eastern Federal University · Department of Oil, Gas and Petrochemical Industry

Cells of non-hereditary retinblastoma in the enucleated eye of a 9-year-old boy were studied using classical histological and immunohistochemical methods. It has been established that disturbances in retinal morphogenesis can be associated with a stop in development at the stage of formation of retinal layers. The inner wall of the eyecup after the...

In solid malignant tumors, as well as in hematological tumors, cancer stem cells (CSCs), quiescent, pluripotent, self-renewing neoplastic, also known as tumor initiating cells, have been identified. CSC tumors are resistant not only to chemotherapy, but also to radiation therapy, as well as they play their role in the occurrence of relapses after s...

Red bone marrow samples investigation in deceased COVID-19 patients enabled to identify the phenomena of secondary hemophagocytosis. Analysis of the data showed that phagocytic reactions during infection of patients with SARS-CoV-2 are manifested both in relation to erythrocytes and leukocytes. The data obtained make it possible to expand the strat...

The purpose of this experimental work was to determine the regeneration ability of bone tissue due to modern bioceramics synthesized and obtained using the previously developed method of reactive spark plasma sintering (Spark Plasma Sintering-Reactive Synthesis, SPS-RS). The study was performed on 26 laboratory rabbits - females of the New Zealand...

In 123 human embryos and fetuses of different age groups, in accordance with the requirements of the Russian Ministry of Health and Medical Industry of 29.04.94 No 82 and according to the Nomenclature of Clinical Laboratory Research of the Russian Ministry of Health (Order No 64 of 21.02.2000), in accordance with the Helsinki Declaration (2000-2013...

Was conducted analysis of the relationship between the pa- thology of the structures of the neuroendocrine system and the organ of vision, depending on various risk factors. It is indicated that neurodegenerative processes in the brain and visual system are interconnected, dependent on the level of mela- tonin. This is due not only to the presence...

Using the method of immune histochemistry, the processes of angiogenesis in neural structures in human prenatal ontogenesis were studied. It has been established that under conditions of ischemia and macrophage and neuroglial induction, vasculogenesis occurs in the neural structure of the retina. However, CSF-dependent structures maintain trophic s...

Pathological scarring at the present stage does not have a clear pathogenetic justification, it is characterized by unpredictability and constant growth of keloid scars, the absence of regression for many years and reasonable pathogenetic treatment. Despite the fact that there is the possibility of extirpation of excess tissue, it is almost impossi...

The frequency of complications associated with the use of cosmetic invasive interventions to rejuvenate and correct the shape of various parts of the face requires a deep study of the causes of keloid scarring induction. The paper presents an analysis of clinical material in the age aspect, depending on gender and topography of skin lesions. It was...

По данным анализа современного состояния вопроса механизмов па- тологических процессов, связанных со снижением зрения, проведенном Н.В. Мащенко, (2017), Е. С. Можилевской, И. В. Рева, А. С. Новиковым, Я. О. Садовой (2022) патология сетчатки остается главной причиной необ- ратимого снижения зрения, в основе которой лежит нарушение обменных и восстан...

Using the method of immune histochemistry, the qualitative and quantitative features of gender hormone receptors in the structure of the mucous membrane epithelium of woman cervix in the postmenopausal period were studied and its relationship with the proliferative activity of keratinocytes was explored. The significance of estrogen deficiency in p...

Malignancy in the cervix mucous membrane often develops in the junction zone of the ectocervix to the endocervix, which is the area of transformation of the stratified squamous epithelium into a single-layer cylindrical epithelium. The reasons for the insufficiency of immune protection in the transition zone of the epithelium from multilayer to sin...

With the emerging of new strains of the SARS-CoV-2 coronavirus (such as B.1.1.529 for example), despite numerous studies to create effective vaccines, it becomes obvious that the relevance of studying the pathomorphology of tissue structures with damaged cellular targets has increased manifold. Most knowledge on genes of pathogenicity loses its imp...

Development of methods for targeted treatment aimed at inhibiting angiogenesis in malignant tissues is of great importance for modern oncology. The features of expression of CD34-positive cells at the border of the tumor and malignant tissue were studied. High expression of CD34-positive cells in small and large vessels during malignancy and is in...

With the permission of the Ethics Committee of the Far Eastern Federal University and in accordance with Russian legislation and the Declaration of Helsinki (2013), immunohistochemical studies were carried out and morphological criteria were developed for the readiness of burn wounds for autodermoplasty with predicted efficacy. The aim of the study...

Chung M. K., Zidar D. A., Bristow M. R., et al. (2021) warn that a historic pandemic due to the coronavirus disease, COVID-19, could have negative potential consequences for the cardiovascular system for millions of survivors worldwide [1]. At the present stage, mechanisms of the long-term consequences of COVID-19 disease caused by SARS-COV-2 are n...

To date, in the context of the COVID 19 pandemic, there are rumors and speculations about the consequences of the infection, as well as a concern on growing cancer risk due to vaccines and vaccination. In this study we reviewed the concepts of the viral action on cancer development and analyzed the data on the possibility of the malignant effect of...

A study of the diagnostic value of tissue eosinophil infiltration in the structure of polyps of the human gastrointestinal tract and surrounding tissues was carried out. We investigated intestinal biopsies from 189 patients aged 40–90 years with polyps, cancer, metastases. This retrospective study allows us to note the possibility of using eosinoph...

Analysis of the HPV positive papilloma structures at different levels of pathological process development was carried out. Classical staining of preparations with hematoxylin and eosin was used for immunohistochemical determination of Ki67-positive cells and phenotyping of CD positive cells. We found that the process of papilloma formation begins w...

The study presents an analysis of the available scientific literature and outcomes of clinical trials on biologically active components of the birch Chaga mushroom Inonotus obliquus (I. obliquus). Our experimental and clinical studies were aimed at testing a novel plant-based substance for patients with cancer or tumors of various locations. The pa...

Despite the fact that HPV infection is widespread among the population around the world, more than 150 strains of HPV viruses are known. At the present stage it is still unclear, why HPV-associated oral papillomas in dental patients either spontaneously undergo involution or become malignant. We investigated the quantitative dynamics of Langerhans...

One of the formidable complications of SARS-COV-2 infection leading to death is coagulopathy. The mechanisms of the development of this pathology at the present stage have not been studied, and clinical blood tests indicate that against the background of normal blood clotting indices, only the Ddi-D-dimer protein exceeds the norm many times over. T...

Manuscript number TOXREP_2020_143 Title The impact of carbon nanofibers on freshwater invertebrates of the families gammaridae, ephemerellidae and chironomidae Article type Full Length Article Abstract Carbon nanofibers (CNFs) are widely used in consumer products today. In this study we assessed the effects of CNFs on the state of the digestive sys...

Carbon nanofibers (CNFs) are widely used in consumer products today. In this study, we assessed the effects of CNFs on the digestive system of three freshwater invertebrate species (Gammaridae, Ephemerellidae, and Chironomidae). The aquatic insects Diamesa sp., Drunella cryptomeria, and Gammarus suifunensis were incubated with the CNFs at the conce...

Study of morbid anatomy material from dead patients suffering from HIV-related illnesses, including hepatitis С, provided an opportunity to identify substantial pathological changes in the structural elements liver that suggested other pathogenetic mechanism of development changes in patients with HIV and hepatitis С associated with impaired metabo...

The study presents the results of a study of the inhibitory role of gold nanoparticles, administered subcutaneously to mice line CBA. The migration paths of gold nanoparticles after subcutaneously injection have been studied, and the mechanisms of their influence on the tissue surrounding the injection site of nanoparticles have been examined. Afte...

Diagnosis of sudden death in the young is based on the results of a set of research methods. Immunohistochemical, histochemical and histochemical methods are fundamental to the diagnosis of hidden forms of connective tissue pathology. These methods allow the sequence of initial and irreversible changes in connective tissue structures to be determin...

Fig S3. Isolation and distribution of CC236/CC271/CC320 lineages of S. pneumoniae in Krasnoyarsk, Siberian Russia. The CC236/CC271/CC320 lineages, including ST types (236, 271, 320, 1464, 2323, 7915 and 9659) that were analyzed as shown in Fig. S2, were isolated in a PCV7 trial before and after PCV7 (a, b) and from non‐immunized pediatric patients...

Fig S1‐4. Structures of virulence‐related surface protein genes, cbpA, rrgB, pitA, pitB and psrP, and the new PCR primer design. (a) cbpA. (b) rrgB. (c1) pitA. (c2) pitB. (d1 to d3) psrP. () Phylogenetic tree analysis. () Nucleotide sequence comparison. () Amino acid sequence comparison. () Strain types are shown as ST (CC)/serotype. Strains marked...

Fig S5. Scanning electron electronmicrograph showing PSS. S. pneumoniae, serotype19A/ST320 PFGE α1 type (19A/ST320Kras‐α1, Fig. 2a), was grown on blood agar. Arrow, PSS; arrowhead, PWS.

Fig S4. Scanning electron micrographs showing a colony of ST320/serotype19A S. pneumoniae on blood agar plates. The PFGE α1 type (ST320/19AKras‐α1, Fig. 2a) was examined. The central region of the colony (a) had very heavy biofilms (b and c). (c) Arrow, S. pneumoniae in heavy biofilms. S. pneumoniae at the colony center (near the edge) has PWS. (b)...

Fig S2. Genetic relationships of CC236/CC271/CC320 lineages of S. pneumoniae in Krasnoyarsk, Siberian Russia. Of the 70 strains (Table 1) examined, 26 (37.1%, 26/70) belonged to the complex CC271/CC236/CC320 lineages, including ST types (236, 271, 320, 1464, 2323, 7915 and 9659). (a) ST types and their allelic profiles of the CC271/CC236/CC320 stra...

Table S1. Primers used for the PCR assay

Streptococcus pneumoniae, a common human pathogen, colonizes the nasopharynx and causes diseases including acute otitis media (AOM). Herein, pneumococcal serotype distributions in children before and after PCV7 vaccination and in patients with pneumococcal disease in Siberian Russia (Krasnoyarsk) are reported. Analyses included antimicrobial suscep...

OBJECTIVE. The study investigated methods of improving the results of active surgical treatment of patients with severe burns and at the same time the possibility of determination of optimal terms of autografting and shortening the terms of hospital stay. MATERIALS AND METHODS. Researches were carried out in patients aged from 18 to 60 at the perio...

ST8/SCCmecIV community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has been a common threat, with large USA300 epidemics in the United States. The global geographical structure of ST8/SCCmecIV has not yet been fully elucidated. We herein determined the complete circular genome sequence of ST8/SCCmecIVc strain OC8 from Siberian...

Comparison of the ebh gene and its product between OC8 and USA300 FPR3757. The nucleotide sequence of ebh and deduced amino acid sequence of Ebh were compared between USA300 FPR3757 (upper side) and OC8 (lower side) in A and B, respectively. In A, ebh (OC8) had three synonymous substitutions (black), two non-synonymous substitutions (red), and one...

Structure of φSa3 in strain OC8. φSa3 (OC8) exhibited the highest homology to φSa3 (T0103). The left-side immune evasion cluster (IEC) region of φSa3 (OC8) also exhibited high homology to φSa3 (TW20). Homologous regions are shaded in each comparison. Genes in IEC: scn, the staphylococcal complement inhibitor (SCIN) gene; sak, the staphylokinase (SA...

Possible mechanism for a deletion at the IS256⑪ site in OC8. In this model, we hypothesized ancestor strains of OC8 for a deletion event. An initial ancestor strain (OC8 ancestor 1) lacks IS256, but has att site sequences, similar to USA300 FPR3757; the size of OC8 ancestor 1 DNA flanked by two att sites was estimated to be 63 bp. The first step (s...

The structure of IS256 extrachromosomal circular DNA in the ST239/SCCmecIII HA-MRSA lineage in Russia. In A, the structure of IS256 (OC8) is based on the OC8 genome sequence (GenBank accession number AP017377) and is the same as that described in Fig 4A. In A and B, strains OC3 and 16K were examined for the circle junction of IS256 circular molecul...

ISO) сплавы, содержащие более 1% никеля, признаны токсичными, тем не менее используемые в медицинской практике ортопедические конструкции в большинстве специальных стоматологических сплавов и нержавеющих сталей содержат более 1% никеля. На современном этапе никель-хромовые сплавы используются в ортопедической стоматологии гораздо чаще, чем благород...

Methicillin-resistant Staphylococcus aureus (MRSA) is a common multidrug-resistant (MDR) pathogen. We herein discussed MRSA and its infections in Krasnoyarsk, Siberian Russia between 2007 and 2011. The incidence of MRSA in 3,662 subjects was 22.0% and 2.9% for healthcare- and community-associated MRSA (HA- and CA-MRSA), respectively. The 15-day mor...

We present the results of electron microscopic, microbiological, immunohistochemical, and molecular genetic studies of gastric biopsy specimens taken for diagnostic purposes according by clinical indications during examination of patients with gastrointestinal pathology. Immune homeostasis of the gastric mucosa against the background of infection w...

This paper presents a comparative analysis of the content of immune cells in the lining of dental patients in multiple myeloma and in her absence. The mechanism of osteonecrosis of the jaw authors recognize local changes in mesenchymal-epithelial interactions leading to induced disturbances in the system of specialized and cambium cells, as well as...

This paper presents a comparative analysis of the content of immune cells in the lining of dental patients in multiple myeloma and in her absence. The mechanism of osteonecrosis of the jaw authors recognize local changes in mesenchymal-epithelial interactions leading to induced disturbances in the system of specialized and cambium cells, as well as...

The cells in the skin tumor developing under conditions of persisting papillomavirus infection are morphologically identical to blast cells in a blood smear from a leukemia patient. The cells filling the lesion focus are morphologically and immunohistochemically related to blood stem cells. A mechanism of epithelial layer modification under conditi...

A 15-year-old boy, who had had a furuncle on his femur, developed femoral pyomyositis and osteomyelitis complicated by septic pulmonary embolism. Panton-Valentine leukocidin-positive (PVL+) ST59 methicillin-susceptible Staphylococcus aureus (MSSA) was isolated from pus and blood. Chemotherapy was started with cefazolin, followed by combination ther...

We analyzed local reactions of immune homeostasis in the human skin, in particular, effector immune cells CD68 responsible for antigen presentation, during human papillomavirus infection. Under conditions of long-term papillomavirus infection, CD68 markers were identifi ed only in the connective tissue of the skin (derma) and were completely absent...

The reactions of dermal structures to subcutaneous injections of gold nanoparticles were studied in CBA mice. Routes of the nanoparticles migration after injection and the mechanisms of their effects on the adjacent tissues were studied. Injected nanoparticles were phagocytosed by macrophages; some of them migrated to lymphoid follicles of the lymp...

The effects of multiwalled carbon nanotubes on epitheliocytes of different compartments of the gastrointestinal tract and urothelium of different compartments of the renal nephron were studied in CBA mice. The nanotubes affected mouse gastrointestinal mucosa and renal urothelium. The cell reaction in the macula densa of the renal distal tubules and...

The effects of multiwalled carbon nanotubes on epitheliocytes of different compartments of the gastrointestinal tract and urothelium of different compartments of the renal nephron were studied in CBA mice. The nanotubes affected mouse gastrointestinal mucosa and renal urothelium. The cell reaction in the macula densa of the renal distal tubules and...

Physically transparent cornea, lens, and vitreous body are not transparent from histophysiological viewpoint and hence, cannot directly transmit light to the retina. The lens forms from two primordia in the course of the eye development: ectodermal (for capsular epithelium) and neuroglia (for lenticular stroma). These data suggest that the neurogli...

[This corrects the article on p. e29187 in vol. 7.].

Two distinct classes of methicillin-resistant Staphylococcus aureus (MRSA) are spreading in hospitals (as hospital-acquired MRSA, HA-MRSA) and in the community (as community-acquired MRSA, CA-MRSA). Multilocus sequence type (ST) 239 MRSA, one of the most worldwide-disseminated lineages, has been noted as a representative HA-MRSA. Here, we isolated...

Deletions in drug resistance or virulence genetic traits in 16K, in comparison with TW20. The data of strain TW20 are from GenBank accession number FN433596. Homologous regions are shaded. In A, contigs 41, 14, 85, and 63 (and probably 77) of strain 16K constituted the bulk of the ΦSa3 phage sequence, showing the presence of the sea gene (encoding...

The agr subtyping for the Russian variant strain 49K. In A, PCR primers for agr typing are shown as an arrow. Strains: agr1, agr1 type strain; agr2, agr2 type strain; 49K, the Russian variant strain 49K. Strain 49K produced a product only for primer sets (Pan and agr1), and not for (Pan and agr2), assigning strain 49K as agr1. However, in the other...

Helicobacter pylori in Vladivostok, Far Eastern Russia, was investigated during 2004 to 2009. The genotype cagA(+) vacA(+) (s1/m1 or m2) accounted for 74.7%, with cagA(-) vacA(+) (s2/m2) at 11.2%. The CagA EPIYA type was mainly Western ABC, with minor types (ABCCC and novel AAABC) or non-Western/non-East Asia type (AB). Regarding drug resistance, m...

The dynamics of reparative processes in rat trigeminal ganglion neurons was studied during the early posttraumatic period; somatic location and interactions of neurons with glial cells were evaluated. The neurons and their nucleoli were enlarged during this period, proliferation of glial cell and transcription activity of chromatin increased. The l...

The melanocytes of the arteries feeding the optic nerve were studied by electron microscopy and methods developed by Koelle, Furness and Costa. The regularities of melanocyte distribution in vessel walls, their polymorphism and ultrastructure were investigated. The results demonstrate the interrelationships between pigmented cells and the adrenergi...