Galina Nikolaevna Fedyukina

• PhD (Chemistry)
• Senior Researcher at State Research Center for Applied Microbiology and Biotechnology

Методом ЯМР-релаксации на протонах изучены лиофильно высушенные образцы бычьего сывороточного альбумина (БСА) и комплексов БСА - сахароза, БСА - лактоза, БСА - трегалоза в диапазоне влажности от 1 до 20 %. Зафиксировано появление трансляционной подвижности молекул дисахаридов при возрастании влажности в системах выше 12% на сухую массу. Установлено...

The residual moisture and dry matter content of lyophilized biomass of the yeast Saccharomyces cerevisiae were investigated by means of NMR relaxation techniques. It was shown that the slow component NMR transverse magnetization signal, corresponding to the so-called isolated water, was in fact caused by the interaction of the disaccharide trehalos...

The freeze dried samples of bovine serum albumin (BSA) and BSA-saccharose, BSA-lactose, and BSA-trehalose complexes were studied by the proton NMR relaxation technique in the humidity range from 1 to 20%. The disaccharide molecules were found to assume translational mobility at the humidity higher than 12% per dry weight. It was established that, a...

Acinetobacter baumannii is one of the significant healthcare-associated meningitis agents characterized by multidrug resistance and a high mortality risk. Thirty-seven A. baumannii strains were isolated from thirty-seven patients of Moscow neuro-ICU with meningitis in 2013–2020. The death rate was 37.8%. Strain susceptibility to antimicrobials was...

The spread of multidrug-resistant Gram-negative bacteria, which is associated with the distribution of beta-lactamase genes and class 1 and 2 integrons, is a global problem. In this study, in the Moscow neurosurgery intensive care unit (neuro-ICU), the high prevalence of the above-stated genes was found to be associated with intestinal and tracheal...

Klebsiella pneumoniae is an increasingly important hospital pathogen. Classical K. pneumoniae (cKp) and hypervirulent K. pneumoniae (hvKp) are two distinct evolutionary genetic lines. The recently ongoing evolution of K. pneumoniae resulted in the generation of hybrid hvKP-MDR strains. K. pneumoniae distinct isolates (n = 70) belonged to 20 sequenc...

The purpose of this study was the identification of genetic lineages and antimicrobial resistance (AMR) and virulence genes in Klebsiella pneumoniae isolates associated with severe infections in the neuro-ICU. Susceptibility to antimicrobials was determined using the Vitek-2 instrument. AMR and virulence genes, sequence types (STs), and capsular ty...

Nanomodified surfaces are increasingly used as bactericidal and immunomodulatory tools in the transition to personalized medicine and health-saving technologies. Medical device-associated infections account for a large proportion of hospital-acquired infections. The use of biomedical implants significantly increases the risk of infection of the hum...

The glycoconjugates with BSA (bovine serum albumin) were synthesized using a next saccharide: disaccharide derivative M.leprae PGL-1 (phenolic glycolipid-1); a complex of the disaccharide fragment and the branched hexasaccharide fragment LAM (lipoarabinomannan); diarabinofuranose fragment LAM. These glycoconjugates were used as antigenic components...

Aims: The objective of this study was phenotypic and genotypic characterization of antibacterial-resistant Klebsiella pneumoniae clinical strains isolated in Moscow Transplantology Intensive Care Unit in 2017-2019. Results: Major strains among K. pneumoniae (n = 63) isolated from 30 patients were recognized as extensive drug-resistant (n = 55) pat...

The antibacterial resistance of healthcare-associated infection (HAI) agents is a major public health problem worldwide. Pseudomonas aeruginosa is one of the main HAI agents, especially in intensive care units (ICUs). The present study focuses on the identification of antibacterial resistance genetic determinants and on determining the structure of...

Hybridomas producing monoclonal antibodies (mABs) to the recombinant protein NS1 of the Zika virus synthesized in human embryonic kidney HEK293 (ZV293) cells have been obtained. The specificity of antibodies has been examined by the enzyme-linked immunosorbent assay (ELISA) using the similarly synthesized NS1 proteins of flaviviruses: the Dengue 1–...

Изобретение относится к области биотехнологии, микробиологии и медицины и нацелено на быстрое получение очищенного белка при помощи препаративного электрофореза в денатурирующих условиях с последующей ренатурацией в геле и электороэлюции биологически активного восстановленного белка. Данный способ не изменяет антигенной и функциональной активности...

Сокращенный черновой вариант статьи в журнал «Биоорганическая химия» #1 2019

Получены гибридомы, продуцирующие моноклональные антитела к липополисахариду Burkholderia mallei и Burkholderia pseudomallei. Подобраны оптимальные условия для конъюгации антител с наночастицами коллоидного золота размером 20 нм. Иммуно-дот анализ показал наличие реакции полученных конъюгатов с липополисахаридами клеток сапа и мелиоидоза и отсутств...

Несмотря на серьезные успехи по ликвидации лепры в глобальном масштабе, ежегодно, главным образом в Индии и Бразилии продолжают регистрировать 300-500 тысяч новых случаев. В последние годы в России регистрируют лишь единичные случаи заболевания, однако эндемические очаги заболевания сохранились в ряде стран ближнего зарубежья, включая Казахстан, Та...

Tick-borne borreliosis (Lyme disease-LD) is caused by pathogenic Borrelia spirochetes that is transmitted through bite of Ixodes ticks to humans and animals. In the Russian Federation, borreliosis registered with an index of 6-7 per 100,000 people annually. In reality, LD morbidity in Russia is much higher because Russian strains develop less eryth...

Biointerfaces with a highly sensitive surface designed for specific interaction with biomolecules are essential approaches for providing advanced biochemical and biosensor assays. For the first time, we have introduced a simple AFM-based recognition system capable of visualizing specific bacterial nanofragments and identifying the corresponding bac...

In this work we have investigated biofunctional surfaces on glow discharged mica and their specific binding of bacterial cells and their fragments from aqueous solutions. Atomic force microscopy (AFM) was used to obtain the topography and to probe the mechanical properties of the biological surfaces. The method of quantitative estimation of the lev...

A model system consisting of freeze-dried samples of bovine serum albumin in combination with trehalose (2:1), and specimens of Escherichia coli К-12 and vaccine culture Francisella tularensis 15/3 cells with various water contents (from 1% to 25%) have been studied using H1-NMR-relaxation method. Correlation between NMR-characteristics (Н1 (slow)...

Методом ЯМР-релаксации на протонах изучены модельные лиофильно высушенные образцы бычьего сывороточного альбумина в композиции с трегалозой (2:1), а также образцы клеток бактерий Escherichia coli К-12 и Francisella tularensis, вакцинный штамм 15/3, в диапазоне влажности от 1 до 25 %. Установлена взаимосвязь между амплитудами медленной Н1 и быстрой...

Atomic force microscopy (AFM) is a type of probe microscopy based on the interaction force between atoms (more precisely, exchange interaction between atoms of the probe and of the specimen). AFM has been used for non-specific and specific visualization of viruses and bacteria. A novel method of specific visualization has been developed using antib...

Frozen-dried preparations were studied for the impact of moisture content of the nuclear magnetic resonance parameter that was a ratio of the amplitudes of a slow component and a rapid one of a free induction signal from the protons of samples. It was found that there was a linear correlation between this parameter and the moisture content of biolo...

Frozen-dried preparations were studied for the impact of moisture content of the nuclear magnetic resonance parameter that was a ratio of the amplitudes of a slow component and rapid one of a free induction signal from the protones of samples. It was found that there was a linear correlation between this parameter and the moisture content of biolog...

Frozen-dried preparations were studied for the impact of moisture content of the nuclear magnetic resonance parameter that was a ratio of the amplitudes of a slow component and rapid one of a free induction signal from the protones of samples. It was found that there was a linear correlation between this parameter and the moisture content of biolog...

The residual water and dry matter condition in the lyophilized biomass of the yeast Saccharomyces cerevisiae was studied by NMR-relaxation technique. It was shown that the slow component of the transverse magnetization NMR signal spectrum corresponding to the so-called "isolated mobile water" was caused in fact by the interaction of the disaccharid...

Методами ЯМР-релаксации исследовано состояние остаточной влаги и сухого вещества в лиофилизированной биомассе дрожжей Saccharomyces cerevisiae. Показано, что медленная компонента огибающей сигнала ЯМР поперечной намагниченности, соответствующая так называемой изолированной подвижной воде, в действительности обусловлена взаимодействием дисахарида тр...

Изобретение относится к биотехнологии, в частности к анализу сухих микробных препаратов, ферментов и других протонсодержащих сухих веществ, и может быть использовано микробиологической, фармацевтической, медицинской, пищевой и других областях народного хозяцства. Целью изобретения является расширение диапазона измерений в сторону малых остаточных в...