Gabriela Sepúlveda Jiménez

Gabriela Sepúlveda Jiménez
Instituto Politécnico Nacional | IPN · Departamento de Biotecnología

Dra.

About

38
Publications
13,459
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
591
Citations

Publications

Publications (38)
Article
Full-text available
Bacillus circulans E9 (now known as Niallia circulans) promotes plant growth-producing indole-3-acetic acid (IAA), showing potential for use as a biofertilizer. In this work, the use of a low-cost medium containing industrial substrates, soybean, pea flour, Solulys, Pharmamedia, yeast extract, and sodium chloride (NaCl), was evaluated as a substitu...
Article
Full-text available
Alternaria porri causa la enfermedad mancha púrpura en cebolla y los hongos benéficos del género Trichoderma pueden ser usados para su biocontrol, pero es limitado el conocimiento de los mecanismos de la respuesta de defensa en la interacción de plantas de cebolla con Trichoderma y A. porri. En este estudio se evalúo la actividad de las enzimas de...
Article
Ruta graveolens L. is a plant of agricultural interest due to its biological activity against phytopathogenic microorganism. This activity has been attributed to the presence of alkaloids, furanocoumarins, terpenes and fatty acids. The accumulation of these compounds is affected by environmental factors. The aim of this work was to analyze the seas...
Article
Full-text available
Stevia rebaudiana is an economically important crop species, due to its ability to produce steviol glycosides, which are compounds that are 300 times sweeter than sugar. Additionally, this species produces phenolic compounds and flavonoids, a portion of which accumulate in trichomes. Arbuscular mycorrhizal (AM) symbiosis is the most important mutua...
Article
Full-text available
In plants, phosphorus (P) uptake occurs via arbuscular mycorrhizal (AM) symbiosis and through plant roots. The phosphate concentration is known to affect colonization by AM fungi, and the effect depends on the plant species. Stevia rebaudiana plants are valuable sources of sweetener compounds called steviol glycosides (SGs), and the principal compo...
Article
Leaf blight disease caused by Stemphylium vesicarium affects onion production in several countries; in Mexico the disease has scarcely been documented. In this study, we identified S. vesicarium in onion plants from Mexico with symptoms of leaf blight, and evaluated the potential of Trichoderma asperellum as a biological control agent against S. ve...
Poster
Full-text available
Arbuscular mycorrhizal (AM) symbiosis may uptake water, increase phosphate translocation and induce changes at the photochemical level. It is known that phosphate concentration plays an important role in symbiotic and nutritional development. Additionally, AM symbiosis can generate biotic stress, modifying its photochemical activity [1]. Stevia reb...
Article
Bursera linanoe cell suspension cultures were initiated from callus grown in Murashige and Skoog medium supplemented with naphthalene acetic acid (3.0 mg L-1) and 6-benzylaminopurine (0.5 mg L-1). In flasks, B. linanoe cell cultures grew over a 9 day period, reaching a maximum biomass of 11.16 g DW L-1. Throughout the growth phase, cell viability w...
Article
Species in the fungal genus Trichoderma have been reported to modulate tolerance to heavy metal stress in plants. However, the mechanism for enhanced plant tolerance to copper (Cu) by Trichoderma spp., has received little attention. To evaluate the potential of the fungus Trichoderma asperellum (To strain) to reduce the adverse effects of Cu on pla...
Article
Cedrus atlantica es una planta ornamental utilizada para la decoración de parques y jardines. Con el fin de micropropagar material de calidad de esta planta, se implementó un protocolo de multiplicación in vitro. Para ello, como explantes se usaron las yemas axilares que se desinfestaron con hipoclorito de sodio al 10% por 15 min., tratamiento con...
Article
The objective of this study was to evaluate the growth of onion bulbs and the content of flavonoids and phenolic compounds, when the onion plants had been inoculated with Trichoderma asperellum and grown with different doses of mineral fertilizer. Onion seeds of varieties Red Satan and Crystal White were inoculated with one of three T. asperellum i...
Article
Castilleja tenuiflora Benth. (Orobanchaceae) in vitro cultures are alternative sources of phenylethanoid glycosides (PhGs), promising natural products for chronic diseases treatment because of their extensive range of biological activities. To increase the yields of these bioactive compounds, the identification of factors affecting their biosynthes...
Article
Full-text available
RESUMEN Stevia rebaudiana Bertoni (stevia) es una planta con potencial industrial, debido a que contiene glucósidos diterpenos, que le confieren propiedades edulcorantes. Además, la planta tiene propiedades medicinales y es usada como anticancerígena, antidepresiva, antiviral, antimicrobiana, y el control de la hipertensión y la obesidad, que son m...
Article
Full-text available
RESUMEN Stevia rebaudiana Bertoni (stevia) es una planta con potencial industrial, debido a que contiene glucósidos diterpenos, que le confieren propiedades edulcorantes. Además, la planta tiene propiedades medicinales y es usada como anticancerígena, antidepresiva, antiviral, antimicrobiana, y el control de la hipertensión y la obesidad, que son m...
Article
We evaluated the ability of Trichoderma asperellum Samuels, Lieckfeldt & Nirenberg to induce resistance to the fungal plant pathogen, Sclerotium rolfsii Saccardo, in three onion (Allium cepa L.) varieties. Both the severity of disease and the activities of glucanase, chitinase and peroxidase (enzymes involved in plant resistance) were evaluated in...
Article
Full-text available
The content of total phenolic compounds and flavonoids was determined in methanol extracts of root, stem, leaves, and inflorescences from wild growing and greenhouse cultivated plants of Castilleja tenuiflora. The antioxidant activity in each extract was evaluated using three in vitro models: scavenging of free radicals with 2,2-diphenyl-1-picrylhy...
Article
Full-text available
The effect of copper stress on betacyanin accumulation and guaiacol peroxidase (GPOD) activity in leaves of different age was evaluated in red beet (Beta vulgaris L. var. Crosby Egyptian) plants. In hydroponic culture, plants were treated with 0.3 μM (control), 50 μM, 100 μM, and 250 μM of CuSO4 for 6 days. Copper was taken up and accumulated in ol...
Article
Full-text available
It was evaluated the growth of Azadirachta indica cell suspension in different conditions of oxygen delivery in Erlenmeyer shake flask. Oxygen transfer rate (OTR, kg O2 m–3 day–1) for the closures utilized were: silicone foam (1.04), cotton (0.58), and aluminum foil (0.07). A. indica cells growing during 6 weeks of subculture showed that lower OTR...
Article
Cecropia peltata is popularly known as “guarumbo” in Mexico and is used in traditional medicine for treatment of diabetes mellitus. C. peltata plants were cultivated in a hydroponic system under controlled conditions. Gradients of light (20, 30 and 100μmolm−2 s−1) and nitrate concentrations (13, 2 and 0.2mM) were applied to estimate their effect on...
Article
Full-text available
Arabinogalactan proteins (AGPs) are glycoproteins present at cell surfaces. Although exact functions of AGPs remain elusive, they are implicated in plant growth and development. The aim of this study was to evaluate the role of AGPs in the process of cell aggregation of Beta vulgaris L. suspension cultures. It was observed that B. vulgaris suspensi...
Article
The aim of this study was to determine if the increase of the initial sucrose concentration (ISC) improves cell growth and arabinogalactan protein (AGP) secretion of Beta vulgaris L. cultures. ISC tested were 43.8, 87.6 and 131.4mM. Cell growth and specific growth rate were improved increasing the ISC. Cell cultures grown with ISC 43.8mM were fed w...
Article
Tecoma stans is a tropical plant from the Americas. Antioxidant activity and both phenolic compound and flavonoid total content were determined for callus tissue of T. stans cultured in either a set photoperiod or in darkness. Callus lines from three explant types (hypocotyls, stem, and leaf) were established on B5 culture medium supplemented with...
Article
Full-text available
Aerial parts of Justicia spicigera are used in Mexican traditional medicine. Antioxidant activity of aqueous and methanolic extracts obtained from leaf, stem and flower of J. spicigera and their contents of phenolic compounds and flavonoids were evaluated in this study. Antiradical activity was proven for the capacity to scavenge the DPPH radical o...
Article
Full-text available
The arabinogalactan proteins (AGPs) are macromolecules found in practically all plant organs, being associated with several aspects of the plant growth and development. These molecules contain carbohydrates and proteins in a 9:1 relation. The carbohydrate moiety is composed mainly of type II arabinoga-lactans, whereas the protein has particular ami...
Article
Full-text available
Las proteínas arabinogalactanos (AGPs) son macromoléculas que se encuentran prácticamente en todos los órganos de las plantas, siendo asociadas con varios aspectos del crecimiento y desarrollo vegetal. Estas moléculas se caracterizan bioquímicamente por contener carbohidratos y proteínas en relación 9:1. El carbohidrato está compuesto principalment...
Article
Uncaria tomentosa cell suspension cultures were grown in a 2-L stirred tank bioreactor operating at a shear rate gamma(.)(avg)=86 s(-1). The cultures showed an early monophasic oxidative burst measured as H2O2 production (2.15 micromol H2O2 g(-1) dw). This response was followed by a transient production of monoterpenoid oxindole alkaloids (178 +/-...
Article
Full-text available
Mechanical wounding, infiltration with P. syringae or A. tumefaciens, and exposure to an H2O2-generating system (Glc/Glc oxidase) induce betacyanin synthesis in red beet (Beta vulgaris) leaves. These conditions also induced the expression of BvGT, a gene encoding a glucosyltransferase (GT) from Beta vulgaris. BvGT has a high similarity to Dorothean...
Article
Red beet (Beta vulgaris) accumulates betacyanins, mainly betanin, in the store root. We report here that in leaves, the synthesis of betacyanins is induced by wounding or infiltration with an avirulent Pseudomonas syringae pv. tabaci or Agrobacterium tumefaciens. A red-violet pigmentation characteristic of betacyanin bordered the damaged tissue in...
Article
Full-text available
The synthesis and the accumulation of low molecular weight compounds known as secondary metabolites are induced as part of the chemical defense response to wounding and/or pathogen attack. During the hypersensitive response, some compounds such as alkaloids, terpenoids, and phenylpropanoids participate killing the pathogen and restricting its invas...
Article
Full-text available
In this work we studied the production of betalains by suspension culture of Beta vulgaris. The B. vulgaris grew at a doubling time of 4.8 days. The betalains production was found to be not-growth related. The culture's response to different carbon sources was investigated. Best growth and pigment production was obtained with sucrose.

Network

Cited By