Gábor Járvás

University of Pannonia, Veszprém · Research Institute of Biomolecular and Chemical Engineering

In this paper, we report on the utilization of micro-technology based tools to fight viral infections. Inspired by various hemoperfusion and immune-affinity capture systems, a blood virus depletion device has been developed that offers highly efficient capture and removal of the targeted virus from the circulation, thus decreasing virus load. Singl...

Protein therapeutics have recently gained high importance in general health care along with applied clinical research. Therefore, it is important to understand the structure–function relationship of these new generation drugs. Asparagine-bound carbohydrates represent an important critical quality attribute of therapeutic glycoproteins, reportedly i...

The role of nitrite (NO2−) and nitrate (NO3−) is essential in the global nitrogen cycle. Monitoring their concentration in environmental and industrial aqueous samples, surface water, soil, food and agricultural products are of high importance. Especially, the effect of anthropogenic emission, i.e., intensified agriculture is essential due to the o...

Structural transformations and lattice expansion of oleate‐capped iron oxide nanocube superlattices are studied by time‐resolved small‐angle X‐ray scattering (SAXS) during solvent removal. The combination of conductor‐like screening model for real solvents (COSMO‐RS) theory with computational fluid dynamics (CFD) modeling provides information on th...

Currently, diagnosing type 2 diabetes (T2D) is a great challenge. Thus, there is a need to find rapid, simple, and reliable analytical methods that can detect the disease at an early stage. The aim of this work was to shed light on the importance of sample collection options, sample preparation conditions, and the applied capillary electrophoresis...

Background: It is well known that more than 90% of cancer deaths are due to metastases. However, the entire tumorigenesis process is not fully understood, and it is evident that cells spreading from the primary tumor play a key role in initiating the metastatic process. Tumor proliferation and invasion also elevate the concentration of regular and...

Prostate cancer represents the second highest malignancy rate in men in all cancer diagnoses worldwide. The development and progression of prostate cancer is not completely understood yet at molecular level, but it has been reported that changes in the N-glycosylation of prostate-specific antigen (PSA) occur during tumor genesis. In this paper we r...

Capillary electrophoresis-based glycan separation and structure identification is one of the most frequently used tools in almost all fields of biological-medical research and biopharmaceutical manufacturing. In this chapter, recent advances in database search assisted glycan structure identification techniques are discussed. The underlying theory...

The utilization of N-glycan profiling recently gained high importance both in fundamental biomedical and applied clinical research. However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies. In th...

he human serum N-glycome is a valuable source of biomarkers for malignant diseases, already utilized in multiple studies. In this paper, the N-glycosylation changes in human serum proteins were analyzed after surgical lung tumor resection. Seventeen lung cancer patients were involved in this study and the N-glycosylation pattern of their serum samp...

Human milk is a complex, dynamically changing biological fluid, which contains a large amount of non-conjugated carbohydrates, referred to as human milk oligosaccharides (HMOs). These HMOs are very important for the infants as they play important roles in the formation of the gut microbiome, the immune system and support brain development. HMOs sho...

Prostate cancer has the highest malignancy rate diagnosed in men worldwide. Albeit, the gold standard serum prostate-specific antigen (PSA) assays reduced the mortality rate of the disease, the number of false positive diagnoses steeply increased. Therefore, there is an urgent need for complementary biomarkers to enhance the specificity and selecti...

A novel N-glycan enrichment strategy is presented using unexpected but strong interactions between the sulfonate groups brought by the fluorescent dye of glycans and the Zr⁴⁺ modified poly(ethylene glycol methacrylate phosphate (EGMP)-co-acrylamide (AM)-co-bis-acrylamide (BAA)) monolith. The poly (EGMP-co-AM-co-BAA) monolith was synthesized via ult...

Aims Demonstrating the capabilities of our new capillary electrophoresis – mass spectrometry method, which facilitates highly accurate relative quantitation of modification site occupancy of antibody-ligand (e.g., antibody-drug) conjugates. Background Antibody-drug conjugates play important roles in medical discovery for imaging and therapeutic in...

The reactions of hydrogen atoms and hydroxyl radicals with benzene and 10 monosubstituted benzenes were investigated by measuring transient absorption spectra, calculating Gibbs free energies, absorption spectra of intermediates formed and determining the rate constants of H• reactions. The basic reaction is addition to the aromatic ring forming cy...

Chronic obstructive pulmonary disease (COPD) and lung cancer are two major diseases of the lung with high rate of mortality, mostly among tobacco smokers. The glycosylation patterns of various plasma proteins show significant changes in COPD and subsequent hypoxia, inflammation and lung cancer, providing promising opportunities for screening aberra...

Protein glycosylation is the most complex and prevalent post-translation modification in terms of the number of proteins modified and the diversity generated. To understand the functional roles of glycoproteins it is important to gain an insight into the repertoire of oligosaccharides present. The comparison and relative quantitation of glycoforms...

GUcal is a standalone application for automatically calculating the glucose unit (GU) values for separated N-glycan components of interest in an electropherogram and suggests their tentative structures by utilizing an internal database. We have expanded the original database of GUcal by integrating all publicly available capillary electrophoresis d...

Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only. Since they require different treatments, it is important to find non-invasive methods capable to readily diagnose them. Moreover, COPD increases the risk of lung cancer development, leading to t...

Glycosylation is considered as one of the crucial critical quality attributes of therapeutic glycoproteins and for their biosimilar counterparts. Carbohydrate moieties of such biopharmaceuticals should be closely monitored during all stages of development and manufacturing and studied accordingly during comparability or similarity exercises. In the...

By coupling a sample pretreatment technique of sample clean up and enrichment power with capillary electrophoresis (CE) of high-performance separation, the task of analyzing trace analytes in a complex matrix such as a biological sample can be carried out successfully with ease. This review aims for providing an overview of strategies to couple sam...

As glycomics research is gaining momentum in the biopharmaceutical industry, there is an increasing need for reproducible high throughput glycoanalytical methods to monitor and characterize the N-glycosylation of therapeutic glycoproteins. Since the glycosylation pattern of glycobiotherapeutics influences their important biological functions, appro...

Mátyás et al. recently published their paper entitled as “Decision support algorithm for the selection of analytical methods in organic compounds detection for future extraterrestrial exploratory missions”, which we found interesting. However, there are some points of the developed method that need to be refined in order to get a practical tool for...

In the presented study, COSMO-RS sigma moments were utilized in a nonlinear multivariate QSPR model for the estimation of the temperature dependent surface tension of various ionic liquids in wide surface tension (15.5–65.1 mN m⁻¹) and temperature (268–533 K) range. The developed model is supposed to be a generally applicable, robust and accurate m...

Capillary electrophoresis connected to electrospray ionization mass spectrometry is a promising combination to analyze complex biological samples. The use of sheathless electrospray ionization interfaces, such as a porous nanoelectrospray capillary emitter, requires the application of forward flow (either by pressure or electroosmosis) to maintain...

The effect of air plasma exposure time on the surface energies and acid-base characteristics of polydimethylsiloxane (PDMS) particles was studied. Polymerized PDMS powder was radio frequency induced air plasma irradiated for 2–10 s with the power of 500 W. The efficiency of the plasma treatments was investigated by a new generation inverse gas chro...

Databases which store and manage long-term scientific information related to life science are used to store huge amount of quantitative attributes. Introduction of a new entity attribute requires modification of the existing data tables and the programs that use these data tables. A feasible solution is increasing the virtual data tables while the...

Significance: Biopharmaceuticals have seen something of tremendous development in recent years, which governs the parallel blooming of analytical glycomics. One of the frequently used methods for the analysis of complex carbohydrates is capillary electrophoresis with laser induced fluorescent detection (CE-LIF). CE-LIF is a high resolution separat...

As a continuation of our previously published work, this paper presents a detailed evaluation of a microfabricated cell capture device utilizing a doubly tilted micropillar array. The device was fabricated using a novel hybrid technology based on the combination of proton beam writing and conventional lithography techniques. Tilted pillars offer un...

A theoretical understanding of the microstructure of organic semiconducting poly- mers and blends is vital to further advance the optoelectronic device performance of organic electronics. We outline the theoretical framework of a combined numerical approach based on polymeric solution theory to study the microstructure of poly- mer:small molecule b...

Despite the ever growing use of capillary electrophoresis in biomedical research and the biopharmaceutical industry, the de-velopment of data interpretation methods is lagging behind. In this paper we report the design and implementation of a co-injected triple-internal standard method, to alleviate the need of an accompanying run of the maltooligo...

Biopharmaceuticals, especially therapeutic monoclonal antibodies, have emerged as a very promising new generation of protein-based drugs. However, their comprehensive analysis continues to pose new challenges for the bioanalytical field. Hyphenation of capillary electrophoresis with electrospray ionization (CE-MS) is a promising technique to addres...

The separation mechanism of charged-fluorophore (aminopyrenetrisulfonate) labeled maltooligosaccharides with α1-4 linkage was studied in polyethylene oxide solutions (MW 300,000 Da) with special interest to possible analyte and/or network deformations as well as potential solute-matrix interactions. The electrophoretic mobilities of the APTS labele...

The solubility of organic semiconductors in environmentally benign solvents is an important prerequisite for the widespread adoption of organic electronic appliances. Solubility can be determined by considering the cohesive forces in a liquid via Hansen solubility parameters (HSP). We report a numerical approach to determine the HSP of fullerenes u...

The determination of absolute solubility was done by preparing oversaturated solutions of the fullerenes in binary solvent blends, followed by stirring for 24 hours at room temperature. Afterwards, the samples were centrifuged at 10000 rpm for five minutes. The centrifuged saturated solutions were subsequently diluted to allow measurements of the o...

Databases which store and manage long-term scientific information related to life science are used to store huge amount of quantitative attributes. Introduction of a new entity attribute requires modification of the existing data tables and the programs that use these data tables. The solution is increasing the virtual data tables while the number...

For the unique identification more attributes (columns) are applied in rational databases. In practice, attributes within a type are defined in same data table. Introducing new entity attributes requires the modification of the existing data tables and applications that use these data tables. In JT logic based databases, records with the same ID va...

Investigation of protein glycosylation is an important area in biomarker discovery and biopharmaceutical research. Alterations in protein N-glycosylation can be an indication of changes in pathological conditions in the medical field or production parameters of biotherapeutics. Rapid development of these disciplines calls for fast, high-throughput...

Databases which store and manage long-term scientific information related to life science are used to store huge amounts of quantitative attributes. Introduction of a new entity attribute requires modification of the existing data tables and the programs that use these data tables. The solution is increasing the virtual data tables while the number...

Temperature dependent differential migration shifts were studied in capillary electrophoresis between linear (maltooligosaccharides) and branched (sialylated, neutral and core fucosylated biantennary IgG glycans) carbohydrates. Background electrolytes without as well as with low and high molecular weight additives (ethylene glycol, linear polyacryl...

The activation energy related to the electromigration of oligosaccharides can be determined from their measured electrophoretic mobilities at different temperatures. The effects of a viscosity modifier (ethylene glycol) and a polymeric additive (linear polyacrylamide) on the electrophoretic mobility of linear sugar oligomers with α1-4 linked glucos...

Design, fabrication, integration and feasibility test results of a novel microfluidic cell capture device is presented, exploiting the advantages of proton beam writing to make lithographic irradiations under multiple target tilting angles and UV lithography to easily reproduce large area structures. A cell capture device is demonstrated with a uni...

Recent emergence in the use of monoclonal antibody therapeutics and other glycoprotein biopharmaceuticals requires high-throughput, robust and automated techniques for their glycosylation analysis. Capillary electrophoresis (CE) is one of the high performance methods of choice; however, while the necessary instrumentation is well developed, the rel...

A new single droplet evaporation model for ethanol-blended gasoline is presented. The real liquid mixture model based on the quantum chemical ab initio description of non-ideality of liquid phase without need of any adjustable parameters. The multi-scale model applies the COSMO-RS theory (micro level) for the estimation of vapor-liquid equilibrium...

A microfabricated pneumatic electrospray nebulizer has been developed and evaluated using computer simulations and experimental measurements of the mass spectrometry signals. The microdevice under development is designed for electrospray mass spectrometry interfacing without the need to fabricate an electrospray needle and can be used as a disposab...

A microfluidic cell capture device was designed, fabricated, evaluated by numerical simulations and validated experimentally. The cell capture device was designed with a minimal footprint compartment comprising internal micropillars with the goal to obtain a compact, integrated bioanalytical system. The design of the device was accomplished by comp...

In this work, a nonlinear multivariate QSPR model based on the COSMO-RS sigma moments was presented for the estimation of the temperature dependent surface tension of various organic compounds in wide surface tension (0.07–45.08 mN m−1) and temperature (283–373 K) range. 1500 data points were used to establish, validate and test the model. An artif...

Capillary electrophoresis hyphenated with electrospray mass spectrometry (CE-ESI-MS) has emerged in the past decade as one of the most powerful bioanalytical techniques. As the sensitivity and efficiency of new CE-ESI-MS interface designs are continuously improving, numerical modeling can play important role during their development. In this review...

In this paper, we review different aspects of computer modeling and simulation of lab-on-a-chip type bioanalytical devices, with special emphasis on cell sorting and rare cell capture, such as circulating tumor cells (CTCs). We critically review important fundamental concepts and innovative applications in addition to detailed analysis by multiphys...

The evaporation flux and the lifetime of nonventilated droplets of multicomponent liquids depend on their physicalchemical properties and the ambient parameters. This work proposes reliable estimation methods, which can be used for the prediction of key input parameters of quasiequilibrium evaporation models in the lack of correct experimental data...

A novel microfluidic cell capture device design is investigated by means of computational fluid dynamics (CFD) simulation. The device features an array of cylindrically shaped microposts. The physical object is simulated in 2D. The model is based on the laminar form of the Navier-Stokes equation assuming one phase flow with same physical characteri...

A new layer evaporation model for multi-component real liquid mixtures, based on activity coefficient calculation on quantum chemical basis is presented. The proposed method applies the COSMO-RS theory for the estimation of vapour–liquid equilibrium of non-ideal solutions and the Maxwell–Stefan diffusion and convection theory for the calculation of...

New QSPR multivariate nonlinear models based on artificial neural network (ANN) were developed for the prediction of the components of the three-dimensional Hansen solubility parameters (HSPs) using COSMO-RS sigma-moments as molecular descriptors. The sigma-moments are obtained from high quality quantum chemical calculations using the continuum sol...