Furio Gramatica

Furio Gramatica
Fondazione Don Carlo Gnocchi | Don Gnocchi · Development & Innovation Dept.

Subnuclear physics

About

50
Publications
8,952
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1,609
Citations
Citations since 2017
7 Research Items
928 Citations
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2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150

Publications

Publications (50)
Article
Here we describe a simple approach for the simultaneous detection of multiple microRNAs (miRNAs) using a single nanostructured reagent as surface plasmon resonance imaging (SPRi) enhancer and without using enzymatic reactions, sequence specific enhancers or multiple enhancing steps as normally reported in similar studies. The strategy involves the...
Article
Virtual reality for the treatment of motor impairment is a burgeoning application of digital technology in neurorehabilitation. Virtual reality systems pose an opportunity for health care providers to augment the dose of task-oriented exercises delivered both in the clinic, and via telerehabilitation models in the home. The technology is almost exc...
Article
The use of exosomes for diagnostic and disease monitoring purposes is becoming particularly appealing in biomedical research because of the possibility to study directly in biological fluids some of the features related to the organs from which exosomes originate. A paradigmatic example are brain-derived exosomes that can be found in plasma and use...
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Full-text available
Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation and characterisation procedures. This study shows for the first time the successful appl...
Article
Multiple ligand presentation is a powerful strategy to enhance the affinity of a probe for its corresponding target. A promising application of this concept lies in the analytical field, where surface immobilized probes interact with their corresponding targets in the context of complex biological samples. Here we investigate the effect of multiple...
Article
Full-text available
Gold nanorods are an important kind of nanoparticles characterized by peculiar plasmonic properties. Despite their widespread use in nanotechnology, the synthetic methods for the preparation of gold nanorods are still not fully optimized. In this paper we describe a new, highly efficient, two-step protocol based on the use of hydroquinone as a mild...
Conference Paper
The detection of biomarkers by means of Surface Enhanced Raman Spectroscopy (SERS) is foreseen to became a very important tool in the clinical practice because of its excellent sensitivity and potential for the simultaneous detection of multiple biomarkers. In the present paper we describe how it was possible to build a sensor for the detection of...
Article
Full-text available
Surface-enhanced Raman spectroscopy (SERS) widely improves the sensitivity of traditional Raman analysis, thus allowing this technique to be exploited for the development of new bio-analytical tests. In this work, 3D substrates made of zinc oxide tetrapods (ZnOTP) are decorated with branched gold nanoparticles by means of a new photochemical approa...
Article
Full-text available
In this article, we describe how it is possible to tune the size and the aspect ratio of gold nanorods obtained using a highly efficient protocol based on the use of hydroquinone as a reducing agent by varying the amounts of CTAB and silver ions present in the “seed-growth” solution. Our approach not only allows us to prepare nanorods with a four t...
Article
Full-text available
In order to minimize the impact of systemic toxicity of drugs in the treatment of local acute and chronic inflammatory reactions, the achievement of reliable and efficient delivery of therapeutics in/through the skin is highly recommended. While the use of nanoparticles is now an established practice for drug intravenous targeted delivery, their tr...
Article
In the clinical practice, the diagnosis and classification of acute myeloid leukaemia (AML) and myelodysplastic syndrome (MDS) start from the manual examination of stained smears of bone marrow (BM) and peripheral blood (PB) by using an optical microscope. This step is subjective and scarcely reproducible. Therefore, the development of subjective a...
Article
In our study, 2-D nanopillar arrays with plasmonic crystal properties are optimized for Surface Enhanced Raman Spectroscopy (SERS) application and tested in a biochemical assay for the simultaneous detection of multiple genetic leukemia biomarkers. The special fabrication process combining soft lithography and plasma deposition techniques allows ta...
Article
Among polymeric nanoparticles designed for cancer therapy, PLGA nanoparticles have become one of the most popular polymeric devices for chemotherapeutic-based nanoformulations against several kinds of malignant diseases. Promising properties, including long-circulation time, enhanced tumor localization, interference with "multidrug" resistance effe...
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Full-text available
We present here the surface-enhanced Raman spectroscopic (SERS)-based detection of the Wilm’s tumor gene (WT1) sequence using dye-labeled reporter oligonucleotide and magnetic core @ gold shell nanoparticles. Thiolated single-stranded DNA (ssDNA) complementers of the WT1 sequence were used to functionalize the gold shell with capture oligonucleotid...
Article
The Wilms tumor gene (WT1) is a biomarker overexpressed in more than 90% of acute myeloid leukemia patients. Fast and sensitive detection of the WT1 in blood samples would allow monitoring of the minimal residual disease during clinical remission and would permit early detection of a potential relapse in acute myeloid leukemia. In this work, Surfac...
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Full-text available
Acute myeloid leukemia (AML) is a proliferative neoplasm, that if not properly treated can rapidly cause a fatal outcome. The diagnosis of AML is challenging and the first diagnostic step is the count of the percentage of blasts (immature cells) in bone marrow and blood sample, and their morphological characterization. This evaluation is still perf...
Article
Aims: Chronic lung allograft dysfunction represents the main cause of death after lung transplantation, and so far there is no effective therapy. Mesenchymal cells (MCs) are primarily responsible for fibrous obliteration of small airways typical of chronic lung allograft dysfunction. Here, we engineered gold nanoparticles containing a drug in the...
Conference Paper
Surface Enhanced Raman Spectroscopy is a powerful analytical technique that combines the excellent chemical specificity of Raman spectroscopy with the good sensitivity provided by the enhancement of the signal observed when a molecule is located on (or very close to) the surface of suitable nanostructured metallic materials. The availability of che...
Article
In this paper we present a new protocol for the synthesis of Star-Like Gold Nanoparticles (SGNs) by a simple one-step, room temperature procedure not involving the use of seeds or surfactants, that can be performed in seconds in any laboratory without the need of special technologies. These particles exhibited excellent properties for Surface Enhan...
Conference Paper
Full-text available
Surface Enhanced Raman Spectroscopy (SERS) is a popular method in bio-analytical chemistry and a potentially powerful enabling technology for in vitro diagnostics. SERS combines the excellent chemical specificity of Raman spectroscopy with the good sensitivity provided by enhancement of the signal that is observed when a molecule is located on (or...
Article
Full-text available
Among the cytokines involved in the pathogenesis and in the progression of the disease, tumor necrosis factor (TNF)-alpha and interleukin (IL)-17 are the most relevant. A three dimensional model of organotypic human skin cultures is a valuable approach for exposing the whole skin to TNF-alpha and IL-17 as specific proinflammatory stimuli, thus mimi...
Article
Apomorphine is a well-known short-acting dopamine agonist typically used in the treatment of patients with advanced Parkinson's disease. In this work we propose a method for detection of apomorphine in blood plasma through Surface Enhanced Raman Scattering (SERS) technique. Detection can be achieved in a few minutes by simply depositing a drop of t...
Conference Paper
A personalized drug administration method and a more reliable electrical interface for monitoring biopotentials (electroencephalogram, electrocardiogram, electromyography) are pivotal matters in the clinical community. The aim of our research is to design, develop and test innovative transdermal devices, deriving from nano- and micro-technologies,...
Article
Monitoring biosignals, such as in electrocardiography (ECG), electromiography (EMG) and electroencephalography (EEG), is important for a better understanding of the pathological and physiological conditions of human subjects. In clinical practice the recording of biopotentials is carried out in general with wet electrodes, considered as the golden...
Article
Full-text available
In this paper, a straightforward method based on elastic light scattering is shown to provide a sensitive and reliable tool for the quantitative determination of receptor-ligand interactions that occur at the surface of suitably designed core-shell nanoparticles. The assay makes use of monodisperse nanocolloids that have minimal optical contrast wi...
Article
The present work reports on Raman and Surface Enhanced Raman Scattering (SERS) vibrational fingerprints of β-carotene and haemoglobin in fresh whole blood (i.e. right after blood test) with different laser excitations, i.e. visible (514 nm) and near-infrared (NIR, 785 nm). The use of colloidal silver nanoparticles significantly increases the Raman...
Article
Full-text available
Microneedles, before being used for biomedical applications, have to satisfy biological and technical specifications, in particular they must guarantee a sufficient strength during exercise. Although studies on the mechanical characterization on single microneedles are reported in literature, microneedles must be arranged in matrices to be used in...
Conference Paper
Microneedles, before being used for biomedical applications, have to satisfy biological and technical specifications, in particular they must guarantee a sufficient strength during exercise. Although studies on the mechanical characterization on single microneedles are reported in literature, microneedles must be arranged in matrices to be used in...
Article
Apomorphine (aporphine-10,11-diol, CA Registry Number 58004) is a non-narcotic derivative of morphine discovered in 1869 by Mattheisen1 and it is a well-known potent short-acting dopamine agonist at D1 and D2 dopamine receptors, typically used in the treatment of patients with advanced Parkinson's disease (PD). To identify the unknown vibrational s...
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Full-text available
An EEG-based brain-computer system can be used to control external devices such as computers, wheelchairs or Virtual Environments. One of the most important applications is a spelling device to aid severely disabled individuals with communication, for example people disabled by amyotrophic lateral sclerosis (ALS). P300-based BCI systems are optimal...
Article
We present the first results of microstructure replica with re-usable masters in poly vinyl alcohol (PVA). Microneedles of heights ranging from 500 to 1100 μm were fabricated through double-exposure deep X-ray lithography (DXRL) process and successfully replicated. A single PVA master was used as a template to successfully replicate up to 10 PMMA m...
Article
Full-text available
A brain-computer interface (BCI) directly uses brain-activity signals to allow users to operate the environment without any muscular activation. Thanks to this feature, BCI systems can be employed not only as assistive devices, but also as neurorehabilitation tools in clinical settings. However, several critical issues need to be addressed before u...
Article
This work presents a portable transdermal drug delivery system that combines a magnetically actuated micro gear pump with a microneedle array. Micropart fabrication is performed through DXRL and LIGA techniques. The manufacturing process of both micropump and microneedle array are described together with wear issues. Results show that the assembled...
Article
A study of nonequilibrium phonon propagation in a low-temperature bolometric detector with a Monte Carlo code is presented. The simulation of the thermalization process allows us to predict the temperature pulse in a 1 cm3 germanium bolometer detecting 5 MeV alpha-particles.
Article
Electroencephalography (EEG) recordings can be used to help paralyzed patients by replacing functionality of damaged neural con-nections through the aid of a brain–computer interface. Conventional electrodes used for EEG require long skin preparation due to the impedance of the outermost layers of skin. On the contrary, dry spiked electrodes allow...
Conference Paper
Partially or completely paralyzed patients can benefit from advanced neuro-prostheses in which a continuous recording of electroencephalogram (EEG) is required, operating some processing and classification to control a computer (BCI, brain-computer interfaces). Patients are so allowed to control external devices or to communicate simple messages th...
Article
Since the development of methods of electronic communication, clinicians have been using information and communication technologies for the exchange of health-related information. However, the evolution and increased availability of new shared media, such as the internet and virtual reality, are changing the ways in which patients and clinicians re...
Conference Paper
The most recent applications of hadron accelerators to tumour therapy are reviewed and the Italian Hadrontherapy Programme is presented in the framework of what is done and planned in the rest of the world. This programme foresees three independent but coherent activities: (i) the construction in Milano of a large National Centre for Oncological Ha...
Article
A feasibility study has just been completed for a hospital-based hadrontherapy facility to be built in Italy. This facility aims at the treatment of 1000 patients/year in 5 treatment rooms served by a H−/light ion synchrotron producing proton beams with energies ranging from 60 to 250 MeV. Generation of neutron beams for boron neutron capture thera...
Article
The main design issues of the feasibility study for a hospital-based hadrontherapy facility to be built in Italy (Progetto Adroterapia) are described. The facility will aim at the treatment of 1000 patients/year in 5 treatment rooms served by a H-/light ion synchrotron. Generation of neutron beams for boron neutron capture therapy and production of...
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Full-text available
The Proton-Ion Medical Machine Study (PIMMS) group was formed following an agreement between the Med-AUSTRON (Austria) and the TERA Foundation (Italy) to combine their efforts in the design of a cancer therapy synchrotron capable of accelerating either light ions or protons. CERN agreed to support and host this study in its PS Division. A close col...

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