Fumihiko Sato

Fumihiko Sato
Kyoto University | Kyodai · Graduate School of Biostudies

Ph. D
Director, Bioorganic Research Institute, Suntory Foundation; Professor Emeritus, Kyoto University

About

375
Publications
23,541
Reads
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10,804
Citations
Citations since 2016
69 Research Items
4242 Citations
20162017201820192020202120220200400600
20162017201820192020202120220200400600
20162017201820192020202120220200400600
20162017201820192020202120220200400600
Introduction
Molecular characterization of functional differentiation in plant cells; Molecular and cellular breeding of plants; Molecular characterization of secondary metabolism, especially isoquinoline alkaloids and useful metabolite production; Synthetic biology in plant metabolism, especially alkaloid biosynthesis
Additional affiliations
July 2022 - present
Suntory Foundation For Life Sciences
Position
  • Research Director
April 2018 - July 2022
Osaka Prefecture University
Position
  • Visiting Researcher
April 2018 - July 2022
Kyoto Prefectural University
Position
  • Professor Emeritus
Education
April 1977 - May 1979
Kyoto University
Field of study
  • Agricultural Chemistry
April 1975 - March 1977
Kyoto University
Field of study
  • Agricultural Chemistry
April 1971 - March 1975
Kyoto University
Field of study
  • Agricultural Chemistry

Publications

Publications (375)
Article
Plant cell cultures are widely used in the micro-propagation of clonal plants, especially virus-free plants, and in the production of useful metabolites such as paclitaxel. On the other hand, the use of plant cell cultures for the more basic characterization of plant functions is rather limited due to the difficulties associated with functional dif...
Article
Full-text available
Benzylisoquinoline alkaloids, such as the analgesic compounds morphine and codeine, and the antibacterial agents berberine, palmatine, and magnoflorine, are synthesized from tyrosine in the Papaveraceae, Berberidaceae, Ranunculaceae, Magnoliaceae, and many other plant families. It is difficult to produce alkaloids on a large scale under the strict...
Article
Full-text available
Plants produce a large variety of low-molecular-weight and specialized secondary compounds. Among them, nitrogen-containing alkaloids are the most biologically active and are often used in the pharmaceutical industry. Although alkaloid chemistry has been intensively investigated, characterization of alkaloid biosynthesis, including biosynthetic enz...
Article
Full-text available
Land plants produce specialized low-molecular-weight metabolites to adapt to various environmental stressors, such as ultraviolet radiation, pathogen infection, wounding, and animal feeding damage. Due to the large variety of stresses, plants produce various chemicals, particularly plant species-specific alkaloids, through specialized biosynthetic...
Article
Transporters have been used in the production of plant metabolites in microorganisms. This study introduced a tobacco multidrug and toxic compound extrusion transporter, NtJAT1, into alkaloid-producing Escherichia coli cells. NtJAT1 expression enhanced alkaloid production secretion into the medium by 14-fold. Our findings further demonstrate the us...
Article
Full-text available
Background Plants produce a variety of specialized metabolites, many of which are used in pharmaceutical industries as raw materials. However, certain metabolites may be produced at markedly low concentrations in plants. This problem has been overcome through metabolic engineering in recent years, and the production of valuable plant compounds usin...
Article
Full-text available
Microorganisms can be metabolically engineered to produce specialized plant metabolites. However, these methods are limited by low productivity and intracellular accumulation of metabolites. We sought to use transport engineering for producing reticuline, an important intermediate in the alkaloid biosynthetic pathway. In this study, we established...
Chapter
Plants synthesise an extraordinary array of natural products that usually do not play a role in their growth and development and thus are traditionally referred to as secondary metabolites. However, recent advances in plant sciences have revealed that these compounds not only function in response to environmental stimuli but also play more basic ro...
Article
Full-text available
Transcription factors of the WRKY family play pivotal roles in plant defense responses, including the biosynthesis of specialized metabolites. Based on the previous findings of WRKY proteins regulating benzylisoquinoline alkaloid (BIA) biosynthesis, such as CjWRKY1—a regulator of berberine biosynthesis in Coptis japonica —and PsWRKY1—a regulator of...
Preprint
Full-text available
Metabolic engineering of microorganisms to produce specialized plant metabolites has been established. However, these methods are limited by low productivity and the intracellular accumulation of metabolites. Here, we aimed to use transport engineering for producing reticuline, an important intermediate in the alkaloid biosynthetic pathway. We esta...
Article
Full-text available
Genome characterization of California poppy (Eschscholzia californica cv. "Hitoezaki"), which produces pharmaceutically important benzylisoquinoline alkaloids (BIAs), was carried out using the draft genome sequence. The numbers of tRNA and rRNA genes were close to those of the other plant species tested, whereas the frequency of repetitive sequence...
Article
Full-text available
With respect to the biosynthesis of plant alkaloids, that of benzylisoquinoline alkaloids (BIAs) has been the most investigated at the molecular level. Previous investigations have shown that the biosynthesis of BIAs is comprehensively regulated by WRKY and bHLH transcription factors, while promoter analyses of biosynthesis enzyme-encoding genes ha...
Article
C4 plants can fix CO2 efficiently using CO2‐concentrating mechanisms (CCM), but they require additional ATP. To supply the additional ATP, C4 plants operate at higher rates of cyclic electron transport around photosystem I (PSI), in which electrons are transferred from ferredoxin to plastoquinone. Recently, it has been reported that the NAD(P)H deh...
Article
Berberine (BBR) is a protoberberine alkaloid extracted from plants such as Coptis japonica (Ranunculaceae). In a previous report, we demonstrated the existence of a 11-hydroxylation pathway employed by BBR-utilizing bacteria for metabolism of BBR. In the present study, we report the identification of the genes brhA, brhB, and brhC as encoding a mul...
Article
Epigenetic mechanisms allow for transgenerational memory of an ancestor's environment and can affect the gene expression, physiology and phenotype of that ancestor's descendants, independent of DNA sequence alteration. Among many model organisms, Caenorhabditis elegans has been instrumental in studies of transgenerational inheritance, most of which...
Article
Coptidis rhizome (CR) is a widely used herbal medicine that contains protoberberine-type alkaloids. CR extract exhibits various pharmacologic activities. A previous study reported the isolation of Rhodococcus sp. strain BD7100 as a berberine (BBR)-utilizing bacterium, and the BBR-degradation pathway has been investigated. When we incubated strain B...
Article
Full-text available
This article presents small RNA sequencing data of Caenorhabditis elegans consist of P0 control worms (untreated), P0 worms treated with a plant alkaloid, sanguinarine, and its F3 offspring. The data were analyzed to identify microRNAs that were differentially expressed in both the sanguinarine-treated P0 and its descendants F3 worms. Targets of th...
Article
Full-text available
Natural products from plants are useful as lead compounds in drug discovery. Plant benzylisoquinoline alkaloids (BIAs) exhibit various pharmaceutical activities. Although unidentified BIAs are expected to be of medicinal value, sufficient quantities of such BIAs, for biological assays, are sometimes difficult to obtain due to their low content in n...
Article
Plants grow under threats of environmental changes that could injure cellular viability and damage whole-plant physiology. To defend themselves against such threats, plants induce protective responses, including the production of defense molecules. The red/purple pigment anthocyanin is synthesized upon leaf and fruit development as well as environm...
Article
Full-text available
Plants produce many specific secondary metabolites as a response to environmental stress, especially biological stress. These compounds show strong biological activities and high stability against degradation by microbes and animals. Berberine, a benzylisoquinoline alkaloid, is found in many plant species and has strong antimicrobial activity, and...
Article
Full-text available
Transcription factors control many processes in plants and have high potentials to manipulate specialized metabolic pathways. Transcriptional regulation of the biosynthesis of monoterpenoid indole alkaloids (MIAs), nicotine alkaloids, and benzylisoquinoline alkaloids (BIAs) has been characterized using Catharanthus roseus, Nicotiana and Coptis plan...
Data
Expression analysis of genes encoding novel biosynthetic enzymes in transgenic California poppy cells. (A) The transcript levels of EcCYP1 and EcCYP2 were determined by quantitative RT-PCR. Nine biological replicates were used of each cell line. (B) Correlation analysis between the expression of EcCYP1 and EcCYP2 and the accumulation of total alkal...
Data
The total accumulation of BIAs in both cells and the medium of transgenic California poppy cells. The contents of sanguinarine, chelerythrine, chelirubine, protopine, and 10-hydroxychelerythrine were estimated using the standard curve of authentic sanguinarine. Nine biological replicates were used of each cell line. Asterisks indicate significant d...
Data
Correlation analysis between the expression of genes encoding biosynthetic enzymes and the accumulation of chelerythrine in flasks containing transgenic California poppy cells. Asterisks indicate a significant correlation (df = 18; *P < 0.05, **P < 0.01, ***P < 0.001). (PDF)
Data
Correlation analysis between the expression of genes encoding biosynthetic enzymes and the accumulation of protopine in flasks with transgenic California poppy cells. Asterisks indicate a significant correlation (df = 18; **P < 0.01). (PDF)
Data
Primers for quantitative RT-PCR of target genes. (PDF)
Data
Correlation analysis between the expression of genes encoding biosynthetic enzymes and the accumulation of 10-HC in flasks with transgenic California poppy cells. Asterisks indicate a significant correlation (df = 18; *P < 0.05). (PDF)
Data
Correlation analysis between the expression of genes encoding biosynthetic enzymes and the accumulation of allocryptopine in flasks with transgenic California poppy cells. Asterisks indicate a significant correlation (df = 18; *P < 0.05, **P < 0.01). (PDF)
Data
One-way ANOVA of expression of biosynthetic enzyme-encoding genes in transgenic California poppy cells. The transcript levels of genes were determined by quantitative RT-PCR. The value is the average of results from three biological replicates. The data are shown as the mean ±s.d.; significance was determined with one-way ANOVA with post-hoc Tukey-...
Data
Correlation analysis between the expression of genes encoding biosynthetic enzymes and the accumulation of sanguinarine in flasks with transgenic California poppy cells. Asterisks indicate a significant correlation (df = 18; *P < 0.05, **P < 0.01, ***P < 0.001). (PDF)
Data
Correlation analysis between the expression of genes encoding biosynthetic enzymes and the accumulation of chelirubine in flasks with transgenic California poppy cells. Asterisks indicate a significant correlation (df = 18; *P < 0.05, **P < 0.01, ***P < 0.001). (PDF)
Data
One-way ANOVA of metabolites in transgenic California poppy cell lines. The content of representative alkaloids in the medium (A), cells (B), and both cells and the medium (C) was calculated using the standard curve of sanguinarine. The value is the average of results from three biological replicates. The data are shown as the mean ±s.d.; significa...
Data
Treatment of VC cells with 25 μM sanguinarine affected the expression levels of genes encoding BIA biosynthetic enzymes. The transcript levels of Ec6OMT, Ec4’OMT, EcCYP719A5, EcTNMT, and EcP6H were determined by quantitative RT-PCR. The relative expression levels were estimated by the standard curve method with three technical replicates and were s...
Data
Accumulation of CjWRKY1 protein was not observed in transgenic California poppy cells. Total protein extracts from VC (A) and CjWRKY1-OX (B) cultured cells were used for immunoblot analysis with anti-CjWRKY1 peptide antibodies and anti-α-tubulin antibody. C: the extract of C. japonica cultured cells served as a positive control. An asterisk and arr...
Article
Full-text available
Recently, more studies have aimed at identifying selective peroxisome proliferator-activated receptor gamma (PPARγ) modulators that transactivate the expression of PPARγ-dependent genes as partial agonists to improve diabetic symptoms with fewer side effects compared to classic PPARγ agonists such as thiazolidinediones. We found that dihydrosanguin...
Article
Full-text available
The PsbP protein is an extrinsic subunit of photosystem II (PSII) specifically developed in green-plant species including land plants and green algae. The protein–protein interactions involving PsbP and its effect on oxygen evolution have been investigated in vitro using isolated PSII membranes. However, the importance of those interactions needs t...
Article
California poppy (Eschscholzia californica), a member of the Papaveraceae family, produces many biologically active benzylisoquinoline alkaloids (BIAs), such as sanguinarine, macarpine and chelerythrine. Sanguinarine biosynthesis has been elucidated at the molecular level, and its biosynthetic genes have been isolated and used in synthetic biology...
Article
Full-text available
Sophisticated regulation of gene expression, including microRNAs (miRNAs) and their target genes, is required for leaf differentiation, growth, and senescence. The impact of miR319 and its target TEOSINTE BRANCHED1, CYCLOIDEA, PROLIFERATING CELL NUCLEAR ANTIGEN BINDING FACTOR (TCP) genes on leaf development has been extensively investigated, but th...
Article
The most prominent alkaloid of Chelidonium majus is dihydrocoptisine, revealing the characteristical benzophenanthridine skeleton. Up to now, any information about the enzymes responsible for its biosynthesis in C. majus and the related genes respectively, is lacking. Based on sequence similarities to the corresponding methylenedioxy bridge-forming...
Article
Plants produce a large variety of alkaloids, which have diverse chemical structures and biological activities. Many of these alkaloids accumulate in vacuoles. Although some membrane proteins on tonoplasts have been identified as alkaloid uptake transporters, few have been characterized to date, and relatively little is known about the mechanisms un...
Article
Full-text available
Lipid metabolism modulation is a main focus of metabolic syndrome research, an area in which many natural and synthetic chemicals are constantly being screened for in vitro and in vivo activity. Berberine, a benzylisoquinoline plant alkaloid, has been extensively investigated for its anti-obesity effects and as a potential cholesterol and triglycer...
Article
Benzylisoquinoline alkaloids (BIAs) are a group of plant secondary metabolites that have been identified as targets for drug discovery because of their diverse pharmaceutical activities. Well-known BIAs are relatively abundant in plants and have therefore been extensively studied. However, although unknown BIAs are also thought to have valuable act...
Article
Full-text available
The presence of alkaloids is rather specific to certain plant species. However, berberine, an isoquinoline alkaloid, is relatively broadly distributed in the plant kingdom. Thus, berberine biosynthesis has been intensively investigated, especially using Coptis japonica cell cultures. Almost all biosynthetic enzyme genes have already been characteri...
Article
Full-text available
By concentrating CO2, C4 photosynthesis can suppress photorespiration and achieve high photosynthetic efficiency, especially under conditions of high light, high temperature, and drought. To concentrate CO2, extra ATP is required, which would also require a change in photosynthetic electron transport in C4 photosynthesis from that in C3 photosynthe...
Article
Full-text available
Benzylisoquinoline alkaloids (BIQ) are among the most structurally diverse and pharmaceutically valuable secondary metabolites. A plant-specific WRKY-type transcription factor, CjWRKY1, was isolated from Coptis japonica and identified as a transcriptional activator of BIQ biosynthesis. However, the expression of CjWRKY1 gene alone was not sufficien...
Article
C4 photosynthesis exhibits efficient CO2 assimilation in ambient air by concentrating CO2 around ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) through a metabolic pathway called the C4 cycle. It has been suggested that cyclic electron flow (CEF) around PS I mediated by chloroplast NADH dehydrogenase-like complex (NDH), an alternative pa...
Article
Full-text available
Shikonin derivatives are specialized lipophilic metabolites, secreted in abundant amounts from the root epidermal cells of Lithospermum erythrorhizon. Because they have anti-microbial activities, these compounds, which are derivatives of red naphthoquinone, are thought to serve as a chemical barrier for plant roots. The mechanism by which they are...
Data
Fluorescence excitation and emission wavelengths of shikonin.
Data
Root tip of cultured hairy roots treated with cytochalasin D. (A) Treatment of hairy roots with 10 μM cytochalasin D for 6 h (a), followed by and 2.5% KOH spray (b). Bar = 100 μm. (B) Fluorescence images of root tips treated with cytochalasin D (10 μM) for 1 h. Exposure time is half (25 msec) of Figure 6Ag. Bar = 100 μm (a,b); 50 μm (c,d).
Article
To analyze changes of energy allocation in PSII at non-steady state photosynthesis, the induction and relaxation of non-photochemical quenching of Chl fluorescence was re-evaluated with the use of Arabidopsis thaliana mutants in which the ability to induce non-photochemical quenching was either enhanced (npq2) or suppressed (npq1 and npq4). When da...
Article
Full-text available
Stylopine is a protoberberine-type alkaloid that has potential biological activities. Based on the successful microbial production of (S)-reticuline, we attempted to produce stylopine from (S)-reticuline by the reaction of berberine bridge enzyme, cheilanthifoline synthase (CYP719A5), and stylopine synthase (CYP719A2). Biosynthetic enzyme expressio...