Frederic Strobl

Frederic Strobl
Goethe-Universität Frankfurt am Main · Institute for Cell Biology and Neuroscience

Doctor of Philosophy

About

50
Publications
3,214
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
349
Citations
Citations since 2017
39 Research Items
276 Citations
2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080

Publications

Publications (50)
Preprint
Full-text available
A key problem in development is to understand how genes turn on or off at the right place and right time during embryogenesis. Such decisions are made by non-coding sequences called 'enhancers'. Much of our models of how enhancers work rely on the assumption that genes are activated de novo as stable domains of gene expressions that undergo little...
Article
Full-text available
The Mediterranean fruit fly (medfly), Ceratitis capitata, is an important model organism in biology and agricultural research with high economic relevance. However, information about its embryonic development is still sparse. We share nine long-term live imaging datasets acquired with light sheet fluorescence microscopy (484.5 h total recording tim...
Article
Full-text available
Calcium (Ca2+) elevation is an essential secondary messenger in many cellular processes, including disease progression and adaptation to external stimuli, e.g., gravitational load. Therefore, mapping and quantifying Ca2+ signaling with a high spatiotemporal resolution is a key challenge. However, particularly on microgravity platforms, experiment t...
Article
Full-text available
Background The technical development of imaging techniques in life sciences has enabled the three-dimensional recording of living samples at increasing temporal resolutions. Dynamic 3D data sets of developing organisms allow for time-resolved quantitative analyses of morphogenetic changes in three dimensions, but require efficient and automatable a...
Article
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical sectioning capability and, hence, provide axial resolution, restrict photobleaching and phototoxicity to a fraction of the sample and use cameras to record tens to thousands of...
Article
Full-text available
We present a deterministic workflow for genotyping single and double transgenic individuals directly upon nascence that prevents overproduction and reduces wasted animals by two-thirds. In our vector concepts, transgenes are accompanied by two of four clearly distinguishable transformation markers that are embedded in interweaved, but incompatible...
Article
Light sheet-based fluorescence microscopy offers efficient solutions to study complex processes on multiple biologically relevant scales. Sample chamber-based setups, which are specifically designed to preserve the three-dimensional integrity of the specimen and usually feature sample rotation, are the best choice in developmental biology. For inst...
Article
Full-text available
The genetic control of anterior brain development is highly conserved throughout animals. For instance, a conserved anterior gene regulatory network specifies the ancestral neuroendocrine center of animals and the apical organ of marine organisms. However, its contribution to the brain in non-marine animals has remained elusive. Here, we study the...
Preprint
Full-text available
The genetic control of anterior brain development is highly conserved throughout animals. For instance, a conserved anterior gene regulatory network specifies the ancestral neuroendocrine center of animals and the apical organ of marine organisms. However, its contribution to the brain in non-marine animals has remained elusive. Here, we study the...
Article
Full-text available
ELife digest Researchers frequently use model organisms, such as mice, zebrafish and various insect species, to understand biological processes – with the underlying idea that discoveries made can be applied to other species too. A common technique is genetic manipulation, in which a foreign gene is inserted into the chromosome of an organism. Thes...
Data
Mating procedure results for seven of the thirteen functional AGOC sublines (Non-Lifeact) from the F3 to the F7 generation. Bold entries mark progeny that were used in the subsequent cross. F6-S, F7-O and F7-C are control crosses. No significant differences between the arithmetic means and the theoretical Mendelian ratios were found. See Source Dat...
Data
All 25 vector sequences as Genebank (.gb) and Geneious (.geneious) files compressed into a single zipped folder (.zip).
Data
Raw scores for all mating procedure result tables ordered by transgenic sublines.
Data
Generations. In this table, the F0 to F7 and their characteristics are summarized.
Data
Mating procedure results for six of the thirteen functional AGOC sublines (Lifeact only) from the F3 to the F7 generation. Bold entries mark progeny that were used in the subsequent cross. F6-S, F7-O and F7-C are control crosses. No significant differences between the arithmetic means and the theoretical Mendelian ratios were found. See Source Data...
Data
F2 insert number determination cross. F2 (mO-mC) founder females were mated with wild-type males and the progeny were scored. Segregation of 60% or fewer transgenic descendants was defined as the criterion for one insert. No deviators could be identified.
Data
Transgenic lines and sublines. In total, 7 transgenic lines with 21 sublines were created, that is, 6 proof-of-principle AGOC sublines, 13 functional AGOC sublines and 2 helper sublines. Two of the functional AGOC sublines have been analyzed with live imaging previously, live imaging data for three more is provided in this study.
Data
Fluorescence stereo microscope filter sets. All components were obtained from AHF Analysentechnik, Tübingen, Germany.
Data
F3 homozygous viability crosses. Two F3 (mO-mC) pre-recombination hemizygous siblings were mated and the progeny were scored. Segregation of 70% or more transgenic descendants was defined as the criterion for homozygous viability. Deviators are marked bold.
Data
Insertion junctions. In the Junction column, the piggyBac TTAA insertion/excision target sequence is marked bold.
Data
Mating procedure results for the two proof-of-principle AGOC #5 and #6 sublines from the F3 to the F7 generation with swapped genders as well as with an alternative Cre-expressing homozygous helper subline, ICE{HSP68’NLS-Cre} #2. Bold entries mark progeny that were used in the subsequent cross. F6-S, F7-O and F7-C are control crosses. No significan...
Data
Vector summary. The 24 vectors used/created in this study listed in order of their type. Numbers in square brackets within the Source/molecular cloning column refer to the respective entry. See also (Figure 1—figure supplement 4).
Data
Cloning and inverse PCR primer pairs. Primer pairs are listed in order of appearance in the Materials and methods section and Supplementary file 3. The Applied Biosciences web calculator (www6.appliedbiosystems.com/support/techtools/calc) was used to calculate the melting temperature TM. In case of primers with overhangs, the TM was only calculated...
Data
Metadata and parameter for the long-term live-imaging datasets DS0001-0003.
Article
Full-text available
The red flour beetle Tribolium castaneum has become the second most important insect model organism and is frequently used in developmental biology, genetics and pest-associated research. Consequently, the methodological arsenal increases continuously, but many routinely applied techniques for Drosophila melanogaster and other insect species are st...
Data
Comparison of sequencing results from genomic DNA extracted either from the whole body or from one wing for SB and Prl. (A) Comparison for the SB strain. Vertical black bars represent sequence deviations. Two deviations were found within intron 2. (B) Comparison for the Prl strain. Vertical black bars represent sequence deviations. Two deviations w...
Data
SB white allele (genomic DNA extracted from one wing; 8,506 bp). (TXT)
Data
Comparison of survival rate when either one or both wings were dissected by two experimenters. (A) Quantification of surviving adults from five replicates with ten adults after the dissection of one wing. The sham control animals were also paralyzed, and their elytra were also lifted from the abdomen, but the wing was not dissected. No significant...
Data
Primer group list. ATub’, alpha tubulin 1 upstream regulatory sequence; ExPCR, primer for extraction PCRs; FD, forward; RV, reverse. (XLSX)
Data
Prl white allele (genomic DNA extracted from one wing; 8,372 bp). (TXT)
Data
Comparison of the SB and Prl white gene sequences to the GA-2 standard. (A) Comparison for the SB allele. Vertical black bars represent sequence deviations, horizontal black lines show insertions / deletions. Compared to GA-2, the SB white allele has multiple insertions, deletions and substitutions mainly within intron 6. The deviations within the...
Data
Successful PCRs on genomic DNA extracted from one or both wings by using a commercial DNA extraction kit. (A) Quantification of successful PCRs on the alpha-tubulin 1 upstream regulatory sequence from five replicates with ten adults each using genomic DNA extracted from one or both wings as template. No significant difference was found. Error bars...
Article
Light-sheet-based fluorescence microscopy features optical sectioning in the excitation process. This reduces phototoxicity and photobleaching by up to four orders of magnitude compared with that caused by confocal fluorescence microscopy, simplifies segmentation and quantification for three-dimensional cell biology, and supports the transition fro...
Article
Full-text available
The red flour beetle Tribolium castaneum has become an important insect model organism in developmental genetics and evolutionary developmental biology. The observation of Tribolium embryos with light sheet-based fluorescence microscopy has multiple advantages over conventional widefield and confocal fluorescence microscopy. Due to the unique prope...
Article
Light sheet-based fluorescence microscopy became an important tool in developmental biology due to its high acquisition speed, low photo-bleaching rate and the high survival probability of the specimens. Initially applied to document the embryogenesis of Drosophila melanogaster, it is now used to investigate the embryonic morphogenesis of emerging...
Article
Tribolium castaneum has become an important insect model organism for evolutionary developmental biology, genetics and biotechnology. However, few protocols for live fluorescence imaging of Tribolium have been reported, and little image data is available. Here we provide a protocol for recording the development of Tribolium embryos with light-sheet...
Article
Full-text available
Simple urea compounds ("phurealipids") have been identified from the entomopathogenic bacterium Photorhabdus luminescens, and their biosynthesis was elucidated. Very similar analogues of these compounds have been previously developed as inhibitors of juvenile hormone epoxide hydrolase (JHEH), a key enzyme in insect development and growth. Phurealip...
Article
Die Lichtscheibenfluoreszenz-Mikroskopie schont die Zellen und liefert hochaufgelöste Bilder. Ihre Vorteile kommen insbesondere bei Langzeitaufnahmen von lebenden Proben zum Tragen. „Mehr Licht!“ – so lauteten angeblich die letzten Worte des größten deutschen Dichters und Denkers Johann Wolfgang Goethe an einen seiner treuen Diener. Im Hinblick auf...
Article
Full-text available
Insect development has contributed significantly to our understanding of metazoan development. However, most information has been obtained by analyzing a single species, the fruit fly Drosophila melanogaster. Embryonic development of the red flour beetle Tribolium castaneum differs fundamentally from that of Drosophila in aspects such as short-germ...
Article
Full-text available
Pulmonary arterial hypertension (PAH) is a fatal disease for which no cure is yet available. The leading cause of death in PAH is right ventricular (RV) failure. Previously, the TNF receptor superfamily member fibroblast growth factor-inducible molecule 14 (Fn14) has been associated with different fibrotic diseases. However, so far there is no stud...

Network

Cited By

Projects

Projects (2)
Project
Explore GPU cross-correlation and improve the accuracy of PIV on biological data.