Frédéric Fine

Terres Inovia · Département Transformation et Valorisation des Graines

An optimized proteolysis process was applied to rapeseed meal proteins (RP) and the hydrolysate was separated by membrane filtration allowing the production of highly metal-chelating peptides in the permeate. In order to identify the chemical structure of the most active obtained metal-chelating peptides, immobilized metal affinity chromatography (...

Preventing lipid oxidation, especially with the polyunsaturated fat-based products, is a major concern in sectors as agri-food and cosmetic. Even though the efficiency of synthetic antioxidants has been recognized, both consumers and manufacturers are looking for more innovative, healthy and quality products while rejecting synthetic additives due...

Rapeseed meal proteins (RP) were enzymatically hydrolyzed using three individual proteases (Alcalase, Flavourzyme and Prolyve) and the enzymatic mechanism were studied. Rapeseed hydrolysates were produced under controlled conditions and the Prolyve hydrolysate was separated by membrane filtration. Their capacity to reduce free radicals (by transfer...

Beside oil, oilseed rape (Brassica napus) seeds contains nutritional bioactives such as polyphenols and glucosinolates. However, to date their nutritional properties have been overlooked in the new “double zero” breeds. Seed alcoholic extracts from two B.napus cultivars most contrasting in their phytochemical contents as measured by mass-spectromet...

Rapeseed and sunflower meal are mainly used as animal feed but they can also be considered as a potential source of bioactive phenolic compounds. However, the desolventization/toasting processes that are needed to produce these meals might influence concentration and chemical structure of phenolic compounds, and change their bioactive properties. M...

Novel hydroxyalkyl esters and bis-aryl esters were synthesized from sinapic and caffeic acids and aliphatic α,ω-diols of increasing chain lengths from 2 to 12 carbon atoms. Then, their antiradical reactivity (DPPH assay) and their antioxidant activity in a model oil-in-water emulsion (CAT assay) were evaluated. All the esters showed lower antiradic...

Rapeseed meal (RSM) is a highly abundant and protein-rich by-product from the oilseed industry. Nowadays, RSM is mainly dedicated to animal feed but some studies have shown the possibility to use this raw material as a source of high value-added products such as emulsifiers, biomaterials and protein isolate with bioactive properties. In other respe...

This study was designed to elucidate the biological variation in expression of many metabolites due to environment, genotype, or both, and to investigate the potential utility of metabolomics to supplement compositional analysis for the design of a new resilient cultivar of Brassica napus that can be steady in phytochemicals in different regions in...

Sunflower and rapeseed meals are agro-industrial coproducts that contain high amount of phenolics (1-4 % dry matter), mostly as esters of caffeic acid (CA) and sinapic acid (SA), respectively. The enzymatic hydrolysis of the ester bonds enables to recover the corresponding free phenolic acids that are bioactive compounds and platform molecules for...

Considering the global population growth driven by emerging countries and the increase in nutritional needs of populations, the valorization of protein fractions of plant resources, particularly oilseed crops, appears to be a major issue to answer the demand. Oilseeds are indeed rich in both oil and protein. Historically, oil being the main compoun...

Due to an unfortunate turn of events, the names of the authors appeared incorrectly in the original publication as given names and family names have been reversed. The correct representation of the authors’ names is listed above and below and should be treated as definitive.

Rapeseed (RSM) and sunflower (SFM) meals are highly abundant and protein-rich by-products from the oilseed industry. Besides their basic use as animal feed, they are seen nowadays as interesting raw materials for the production of high value added products such as protein isolates, peptides, emulsifiers and biomaterials. In other respects, they con...

This study investigated alternative solvents: ethanol and isopropanol, to replace hexane and enhance the quality and value of oil and meal. Rapeseed oil extraction was carried out using ethanol (92 wt.% or 96 wt.%), isopropanol (84 wt.% or 88 wt.%) or hexane (as reference). Results show that hydroalcoholic extraction increased meal protein content...

Rapeseed meal is a cheap and abundant raw material, particularly rich in phenolic compounds of biotechnological interest. In this study, we developed a two-step bioconversion process of naturally occurring sinapic acid (4-hydroxy-3,5-dimethoxycinnamic acid) from rapeseed meal into canolol by combining the complementary potentialities of two filamen...

In this study, extraction of soluble proteins from rapeseed cake using different conventional and innovative extraction processes in order to maximize the extraction yield has been investigated. Firstly, various extraction techniques including ultrasound, microwave, and percolation were tested to increase the protein recovery efficiency. Secondly,...

La 107ème réunion annuelle de l’AOCS s’est tenue à Salt Lake City (USA) du 1 au 4 mai 2016. Comme à son habitude, ce congrès a balayé l’ensemble des domaines de la recherche concernant de près ou de loin les lipides avec pas moins de 11 sessions techniques en parallèle. À noter la mise en place pour la première fois d’une véritable session « Protei...

Continuous screw pressing is widely used for mechanical extraction of oil from oilseeds. Despite significant recent advances in the field of press design and automation, it remains difficult to predict performance based on theoretical approaches. This work is devoted to better understand, characterize and model the solid/liquid behavior in a screw...

Majoritairement constituées de triglycérides et de leurs acides gras, les huiles végétales contiennent également entre 1 et 5 % de micronutriments tels que les tocophérols, les phytostérols, les polyphénols et les coenzymes Q. Naturellement présents dans les graines de colza, soja et tournesol, ces composés sont connus pour leurs effets bénéfiques...

The thirtieth congress EuroFedLipid organized by SISSG (Società Italiana per lo Studio delle Sostanze Grasse - Italian society for the study of lipids and fats) held in Firenze from 27th to 30th September 2015. As usual, this congress overviewed research in areas about lipids with up to 11 technical sessions in parallel. The aim of this article is...

Sunflower seed quality, in particular the characteristics of hullability and protein content, has a significant impact on the protein content of the resulting meal. Seeds dehulled before crushing produce a meal with a protein content of approximately 36%; without dehulling, the protein content is typically in the range of 27–29%. This study seeks t...

Le 13e congrès EuroFedLipid organisé par la SISSG (Società Italiana per lo Studio delle Sostanze Grasse –Société italienne pour l’étude des lipides et des corps gras) s’est tenu à Florence (Italie) du 27 au 30 septembre 2015. Comme à son habitude, ce congrès a balayé l’ensemble des domaines de la recherche concernant de près ou de loin les lipides...

Ultrasound-assisted extraction of rapeseed oil was investigated and compared with conventional extraction for energy efficiency, throughput time, extraction yield, cleanness, processing cost and product quality. A multivariate study enabled us to define optimal parameters (7.7 W/cm2 for ultrasonic power intensity, 40 °C for processing temperature,...

In the past few years, much interest has focused on the applications of ultrasound in food processing and technology. Many reports suggest effective benefits from combining ultrasound with classical techniques. Applications of ultrasound generally involve processes that can enhance rates, improve quality and/or safety, and reduce processing time. U...

Diepoxydized diphenyls from eugenol, 4-vinyl guaiacol and 4-vinyl syringol (canolol) were synthesized as sustainable alternatives to the diglycidyl ether of bisphenol A (DGEBA). In the first step, glycidylated derivatives were produced by reaction with epichlorohydrin. Then, the dimerization of these derivatives was performed by cross metathesis (C...

Diepoxydized diphenyls from eugenol, 4-vinyl guaiacol and 4-vinyl syringol (canolol) were synthesized as sustainable alternatives to the diglycidyl ether of bisphenol A (DGEBA). In the first step, glycidylated derivatives were produced by reaction with epichlorohydrin. Then, the dimerization of these derivatives was performed by cross metathesis (C...

Background: Solid-liquid expression or pressing is a unit operation in which liquid is separated from solid–liquid mixture by mechanical compression. This technology is widely used for rapeseed oil extraction and its control is a major issue in the efficiency of the crushing process since the characteristics of the expeller cake such as the mechani...

Rapeseed oil is at the third place of the most consumed vegetable oils worldwide after palm and soya and is at the first place in Europe and France. The implementation of rapeseed oil process includes several steps such as preparation of seeds, mechanical extraction by pressing and solvent extraction of the press cake. This solvent extraction step...

Background: Recently published reports suggest significant amounts of food losses and waste across the world, but reliable data is scarce. In industrialized countries, little attention has been put so far on losses and waste at the upstream stages of food supply chains (primary production, post-harvest operations, processing). A general food losses...

Vegetable oils take a large part in industry for food and non-food applications. However the extraction process of oil from oilseeds includes a solvent extraction step using hexane. Despite its various advantages it presents numerous drawbacks; it is sourced from petroleum, it has a high flammability and it appears to be dangerous for health and en...

Dehulling sunflower seeds can increase meal protein content by up to 35–36%. However, high variability in seed quality constitutes an obstacle to optimizing the dehulling process. This study has sought to identify the parameters that influence the protein content of sunflower seeds and the ease with which they can be dehulled. Genetic, climatic and...

Cold-pressing has well-known adverse effects on solvent extraction performances from the resulting cakes. Here we investigated the added value of an additional pelletizing step in a batch extractor (3.5 kg) to produce pellets with better physical quality (durability, wettability and percolation speed) for hexane extraction and desolventization. We...

Tocopherols, phytosterols, polyphenols, and coenzymes Q are naturally present in oilseeds such as sunflower, rape, and soybean. Besides contributing to taste and color, micronutrients help protect against health disorders such as cardiovascular diseases and cancer. However, during the conventional oil manufacturing process, many minor components ar...

Nowadays, it is essential to restructure various conventional industrial sectors (e.g. food, cosmetic, additive, pulp and paper industries, and bioethanol and biodiesel generation processes) into integrated biorefineries through valorisation of by-products streams. Rapeseed and sunflower are two of the most important oilseed crops in the world, aft...

The current process to extract oil from oilseeds such as rapeseed or sunflower, involves three main steps: a) preparation of the seeds including conditioning and pressing, b) solvent extraction, c) solvent elimination from the oil and the residue. Hexane is commonly used by industry because of well-known technical and economic advantages. Neverthel...

Currently, the most widely used solvent for extraction of vegetable oils is hexane given its various advantages such as ease of removal from the products, low boiling point and good lipid solubility. One of the major constituents of industrial hydrocarbons mixture is n-hexane; it is sourced from fossil resources and registered under the REACH Regul...

In the current context of boosting production of high erucic acid rapeseed, because of the wide range of its industrial applications, this literature review is designed to provide a general overview of available varieties, current knowledge of plant improvement and paths of developing research to increase competitiveness of varieties with high eruc...

INRA has initiated a comprehensive approach to food loss and waste for all the plant and animal sectors, from field to distribution. In this study, all comestibles that leave the human food chain and are not recycled into animal feed are considered as losses and waste. The main French oilseed sectors are studied (rapeseed, sunflower and soya, as we...

Canolol (2,6-dimethoxy-4-vinylphenol) constitutes a potential platform molecule for the synthesis of polymers or fine chemicals. It can be obtained from sinapine (sinapoyl choline), the major phenolic of rapeseed, after hydrolysis of the latter into sinapic acid and its subsequent thermal decarboxylation. As known, the treatment used for rapeseed d...

The present study was designed to evaluate the performance of alternative bio-based solvents, more especially 2-methyltetrahydrofuran, obtained from crop's byproducts for the substitution of petroleum solvents such as hexane in the extraction of fat and oils for food (edible oil) and non-food (bio fuel) applications. First a solvent selection as we...

In the past few years, much interest has focused on the applications of ultrasound in food processing and technology. Many reports suggest effective benefits from combining ultrasound with classical techniques. Applications of ultrasound generally involve processes that can enhance rates, improve quality and/or safety, and reduce processing time. U...

Rapeseed meal is the co-product of the pressing and de-oiling process of rapeseed seeds and is used as animal feed. Most of phenolic compounds remain in the meal after processing the seeds. While sinapine (sinapoyl choline), sinapoyl glucose and sinapic acid are naturally present in the seed, canolol (4-vinylsyringol) is formed during processes of...

Ce numéro présente les travaux conduits dans le cadre de l’analyse des Pertes agricoles et alimentaires dont une synthèse a été présentée lors du colloque du 28 novembre 2015 à Paris.

The fundamental analysis and modeling of kinetics of solvent extraction of rapeseed oil enable the quantification of the "washing" and "diffusion" steps. Both are illustrated through "starting accessibility" and "effective diffusivity", respectively. This is a relevant way to identify how to intensify and optimize the operating conditions in terms...

The recent success of dehulled sunflower meals on the French market encourages reconsideration of the possibility of applying dehulling techniques to rapeseed. Hulls account for 18−20% of rapeseed mass; they contain mostly fibres (72%, 78% and 99%, respectively of the seeds’ NDF, ADF and ADL). Complete removal of these hulls would result in a high...

On a nutritional standpoint, lipids are now being studied beyond their energy content and fatty acid (FA) profiles. Dietary FA are building blocks of a huge diversity of more complex molecules such as triacylglycerols (TAG) and phospholipids (PL), themselves organised in supramolecular structures presenting different thermal behaviours. They are ge...

Les huiles représentent une part importante du régime alimentaire chez l’homme et contiennent de nombreux nutriments essentiels. Les consommateurs se montrent de plus en plus attentifs aux mentions relatives à l’origine et à la qualité des produits qu’ils achètent. Quant aux industries, elles ont compris qu’il n’est désormais plus possible de produ...

Oils represent an important part in human diet and contain essential nutriments. Consumers are more and more swayed by the argument of product origin. As for industries, they understood it’s not possible anymore to produce without thinking about the environment. This study deals with the use of bio-solvents to substitute hexane which the most widel...

L’objectif de cette étude vise à évaluer les performances de cinq solvants alternatifs (deux alcools : éthanol, isopropanol, et les terpènes, d-limonène, α-pinène, p-cymène) par rapport au n-hexane pour l’extraction de l’huile de colza. Les huiles extraites à l’aide de ces différents solvants ont été analysées quantitativement et qualitativement af...

Cooking-pressing or extrusion-pressing processes applied to soybeans produce partially deoiled cake used for animal feeding. These two processes were studied and compared with a view to implementation in an industrial unit. The various factors of hydrothermal treatment for conditioning the beans were studied at different scales (batch from 2 kg to...

Cooking-pressing or extrusion-pressing processes applied to soybeans produce partially deoiled cake used for animal feeding. These two processes were studied and compared with a view to implementation in an industrial unit. The various factors of hydrothermal treatment for conditioning the beans were studied at different scales (batch from 2 kg to...

Rapeseed and sunflower are two of the world's major oilseeds. Rapeseed and sunflower meal (RSM and SFM), the by-products of oil extraction, are produced in large quantities. They are mainly composed of proteins, lignocellulosic fibres and minerals. They were initially used as a protein complement in animal feed rations and sometimes as fertilizer o...

Sunflower seed dehulling increased protein content in meal but the high variability of seed quality was a problem for optimizing industrial process. The aims of this study were: to measure variability of protein content and dehulling ability of sunflower seeds at the entrance of a crushing plant and to assess strategies in order to improve dehullin...

Since 2003, the chemical composition of rapeseed and sunflower meals produced in the French crushing factories was monitored with a quality survey. In the context of bio-diesel production, the rapeseed meal (RSM) and sunflower meal (SM) availability in France was expected to reach 3 million tons in 2011 (increasing by 90 % from 2006). So, this surv...

In order to study the feasibility of an identity preserved (IP) production of soybean meal for feedstuff, two processes based on a thermal treatment (extrusion or cooking) associated to pressing were optimized and compared for oil extraction yield, meal quality and economics. The effects of cooking on the antitrypsic factors (ATF) inactivation and...

From the nutrition point of view, lipids are primarily considered as energy suppliers, their dietary intake having to be limited. In fact, lipids are sources of various fatty acids, and dietary recommendations include the required daily intakes in different fatty acids (fatty acid profile). Beyond these quantitative aspects, fatty acids are part of...

Revue réalisée en hommage à Michel Ollivon (Directeur de Recherche au CNRS, UMR8612, Châtenay-Malabry), décédé en juin 2007, qui a été à l’origine de la création du RMT Listral. Ce Réseau Mixte Technologique avec pour thématique les Lipides Structurés Alimentaires est coordonné par l’ACTIA et animé par Actilait. Les autres partenaires du réseau son...

The process for heat treatment of seeds and food powders patented and developed by our laboratory is based on very short heat stresses (from 0.1 up to 30 s) at very high temperatures (in the range of 200 to 600 -C) followed by an instantaneous cooling due to a cold gas (À 80 -C). The decontamination of dried powders is difficult, and the difficulty...

The thermal treatment of Saccharomyces cerevisiae cells, which were homogeneously incorporated into dried wheat flour particles, was performed for various particle radii (0.8 to 1.6, 1.6 to 2.8, 2.8 to 3.2, and 5 mm) and for an initial water activity of 0.20. A new high-temperature short-time process developed by our laboratory for powder decontami...

Thermal treatment of Bacillus subtilis spores and Saccharomyces cerevisiae cells dried on glass beads was performed at various initial water activities (in the range 0.10-0.90). Experiments were carried out at 150 degrees C, 200 degrees C and 250 degrees C for 5-120 s. Significant destruction of up to 10(7) vegetative cells and up to 10(5) spores g...

To study the factors and mechanisms involved in microorganisms' death or resistance to temperature in low-water-activity environments, a previous work dealt with the viability of dried microorganisms immobilized in thin-layer on glass beads. This work is intended to check the efficiency of a rapid heating-cooling treatment to destroy microorganisms...

The aim of this study was to evaluate the efficiency of pulsed light on the destruction of dried microorganisms on fluidized glass beads and to determine treatment parameters (energy level, water activity, final product quality) for process optimization. The applied drying method allowed microorganisms to remain viable on glass beads or dried powde...

Les produits pulverulents sont largement representes dans les differentes filieres alimentaires et leurs qualites microbiologiques et organoleptiques font l'objet de la plus grande attention de la part des industriels. La faible activite de l'eau des poudres alimentaires contribuent a selectionner une flore resistante et notamment une majorite de f...

To ensure a secure and steady food supply, food needs to be preserved, protected from deterioration, and stabilized so it can be stored for extended periods. Extending food products shelf life requires an inactivation of the living cells, i.e. generally spoilage or toxic microorganisms, they contains and avoiding restart of their proliferation. The...

Pulverulent products are widely represented in food-industries that is why their microbiological and organoleptic qualities are very important parameters for industrials. The low water activity of food powders performs a selection of resistant micro-organisms whose most of them are spore-formers. For brand image reasons, irradiating technology was...