Frédéric Carrière

Frédéric Carrière
French National Centre for Scientific Research | CNRS · UMR7281 Bioénergétique et Ingénierie des Protéines

PhD
Research Director at CNRS, Marseille, France

About

369
Publications
69,672
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Introduction
Frédéric Carrière currently works at the UMR7281 Bioénergétique et Ingénierie des Protéines (BIP), a joint laboratory of French National Centre for Scientific Research and Aix Marseille University. Frédéric is a member of the BIP research group "Enzymology in complex media". He is involved in research projects dealing with the Enzymology of lipolytic enzymes and lipid-protein interactions, with an expertise in Biochemistry, biocatalysis, Interface science and Analytical Chemistry.
Additional affiliations
January 2018 - present
French National Centre for Scientific Research
Position
  • Managing Director
January 2007 - December 2015
Institut Carnot LISA
Position
  • Managing Director
Description
  • Partnership in the field of lipids for industry and health
January 2005 - present
French National Centre for Scientific Research
Position
  • Research Director at CNRS
Education
January 2002 - December 2002
Aix-Marseille Université
Field of study
  • Enzymology
October 1988 - August 1992
Aix-Marseille Université
Field of study
  • Enzymology
September 1981 - July 1986
Ecole Supérieure de Chimie de Marseille
Field of study
  • Chemical Engineering

Publications

Publications (369)
Article
Full-text available
The chloroplast protein CP12, which is widespread in photosynthetic organisms, belongs to the intrinsically disordered proteins family. This small protein (80 amino acid residues long) presents a bias in its composition; it is enriched in charged amino acids, has a small number of hydrophobic residues, and has a high proportion of disorder-promotin...
Article
Full-text available
The structural behavior of model assemblies composed of monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), the two main galactolipids found in plants, was investigated at the air/water interface and in aqueous dispersion. To approach the composition of the natural photosynthetic membranes, tunable Langmuir model membrane of...
Presentation
Full-text available
Specific activities of digestive lipases were measured using rapeseed oil triacylglycerols (TAG), purified 1,3-sn-diacylglycerols (DAG) and 1,2(2,3)-sn-DAG, and a DAGOIL containing 40 % w/w DAG. Gastric lipase was more active on 1,3-sn-DAG than on 1,2(2,3)-sn-DAG and TAG, whereas pancreatic lipase displayed a reverse selectivity with a higher activ...
Article
Full-text available
An in vitro gastrointestinal human digestion model, with and without additional rapeseed oil, was used to measure the bioaccessibility of the major lipophilic nutrients enriched in chloroplasts: β-carotene; lutein; α-tocopherol; and α-linolenic acid. Chloroplast-rich fraction (CRF) material for this work was prepared from post-harvest pea vine fiel...
Article
Full-text available
The chloroplast protein CP12 is involved in the dark/light regulation of the Calvin–Benson–Bassham cycle, in particular, in the dark inhibition of two enzymes: glyceraldehyde−3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK), but other functions related to stress have been proposed. We knocked out the unique CP12 gene to prevent its e...
Article
The specific activities of gastric and pancreatic lipases were measured using triacylglycerols (TAG) from rapeseed oil, purified 1,3-sn-DAG and 1,2(2,3)-sn-DAG produced from this oil, as well as a rapeseed oil enriched with 40% w/w DAG (DAGOIL). Gastric lipase was more active on 1,3-sn-DAG than on 1,2(2,3)-sn-DAG and TAG, whereas pancreatic lipase...
Presentation
Full-text available
Lipases (triacylglycerol acyl hydrolases; EC 3.1.1.3) are water-soluble enzymes acting on an insoluble lipid substrate forming lipid droplets dispersed in biological media (GI tract contents in the course of digestion; cytosol in cells). Digestive lipases, especially pancreatic lipase, are the best known regarding their physiological role and struc...
Article
Conventional degreasing of skins and hides in the leather industry requires high amounts of organic solvents and detergents that cause environmental issues. In this study, the LIP2 lipase from the yeast Yarrowia lipolytica (YLLIP2) was shown to be effective in degreasing sheepskins, thus reducing the amount of harmful chemicals. Using 10 mg of lipa...
Article
Full-text available
Most lipids in our diet come under the form of triacylglycerols that are often redispersed and stabilized by surfactants in processed foods. in plant however, lipid assemblies constitute interesting sources of natural bioactive and functional ingredients. in most photosynthetic sources, polar lipids rich in ω3 fatty acids are concentrated. The obje...
Article
Full-text available
In vitro digestion studies often use animal digestive enzyme extracts as substitutes of human gastric and pancreatic secretions. Pancreatin from porcine origin is thus commonly used to provide relevant pancreatic enzymes such as proteases, amylase and lipase. Rabbit gastric extracts (RGE) have been recently introduced to provide gastric lipase in a...
Article
Galactolipids are the most abundant lipids on earth where they are mainly found in photosynthetic membranes of plant, algae, and cyanobacteria. Pancreatic lipase-related protein 2 (PLRP2) is an enzyme with galactolipase activity allowing mammals, especially herbivores, to digest this important source of fatty acids. We present a method for the quan...
Method
Full-text available
Principe : La chymotrypsine catalyse l'hydrolyse de l'ester ATEE (N-Acetyl-L-Tyrosine Ethyl Ester) qui conduit à la formation d'un acide. Le titrage de l'acide libéré en fonction du temps permet de déterminer l'activité enzymatique de la chymotrypsine. On peut également réaliser la réaction à pH constant grâce à un système expérimental permettant d...
Method
Full-text available
Principe : La trypsine catalyse l'hydrolyse de l'ester BAEE (N-Benzoyl-L-Arginine Ethyl Ester) qui conduit à la formation d'un acide. Le titrage de l'acide libéré en fonction du temps permet de déterminer l'activité enzymatique de la chymotrypsine. On peut également réaliser la réaction à pH constant grâce à un système expérimental permettant d'ajo...
Method
Full-text available
This assay is based on the stop-point assay of hemoglobin degradation developed by Anson (1938). Method: The rate of hydrolysis of denatured hemoglobin by pepsin is measured. One unit releases 0.001 A280 as TCA soluble hydrolysis products per minute at 37°C under the specified conditions.
Chapter
Lipid digestion is a complex process that takes place at the lipid-water interface and involves various lipolytic enzymes present predominantly in the stomach and the small intestine [34]. These enzymes catalyse the hydrolysis of a variety of dietary lipids from animal and plant sources, such as triacylglycerols (TAGs), phospholipids, galactolipids...
Article
In this study, we evaluated vitamin D and mineral (iron, zinc, magnesium) transfer to the bolus aqueous phase during the digestion of meals with/without pulses. We performed in vitro digestions using test meals made either of i) beef, semolina and/or chickpeas, or of ii) potatoes supplemented or not with fibers, phytates, tannins and saponins. Chic...
Article
Background: Few studies have tested the small intestine microbiota in humans, where most nutrient digestion and absorption occur. Here, our objective was to examine the duodenal microbiota between obese and normal volunteers using metagenomic techniques. Methodology/Principal Findings: We tested duodenal samples from five obese and five normal vol...
Article
Background The measurement of pancreatic lipase is important for the diagnosis of feline and canine pancreatitis. Recent studies have claimed that lipase assays using the 1,2‐o‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6’‐methylresorufin) ester (DGGR) as a substrate are more specific for measuring pancreatic lipase than traditional lipase assays. Howev...
Chapter
The involvement of natural deep eutectic solvents (NaDES) in biological processes has been speculated but experimental evidence based on their observation in cells and biochemical reactions is still largely missing. Nevertheless, a sum of observations and analogies between NaDES physical-chemical properties and those of peculiar cellular environmen...
Article
Full-text available
Caatinga is a Brazilian semi-arid ecosystem that stands out for presenting unique environmental characteristics with a dry, spiny and deciduous shrub/forest vegetation with several species that can be renewable oil sources with potential applicability in oleochemical and nutrition. Caatinga oilseeds have a high content of unsaturated fatty acids, p...
Article
Galactolipids, mainly monogalactosyl diglycerides and digalactosyl diglycerides are the main lipids found in the membranes of plants, algae and photosynthetic microorganisms like microalgae and cyanobacteria. As such, they are the...
Article
Full-text available
The anti-malarial drug Chloroquine (CQ) and its derivative hydroxychloroquine have shown antiviral activities in vitro against many viruses, including coronaviruses, dengue virus and the biosafety level 4 Nipah and Hendra paramyxoviruses. The in vivo efficacy of CQ in the treatment of COVID-19 is currently a matter of debate. CQ is a lysosomotrophi...
Article
Full-text available
The link between food and human health is increasingly a topic of interest. One avenue of study has been to assess food disintegration and interactions within the gastrointestinal tract. In vitro digestion models have been widely used to overcome the constrictions associated with in vivo methodology. The COST Action INFOGEST developed an internatio...
Article
Intracellular lipid droplets (LDs) are receiving increasing interest from the scientific community, notably because of their link with metabolic diseases (obesity, diabetes) and the production of lipid-derived biofuels in microorganisms. Understanding the biogenesis and fate of LDs upon energy mobilization is essential to monitor fat storage in adi...
Book
The involvement of natural deep eutectic solvents (NaDES) in biological processes has been speculated but experimental evidence based on their observation in cells and biochemical reactions is still largely missing. Nevertheless, a sum of observations and analogies between NaDES physical-chemical properties and those of peculiar cellular environmen...
Article
Full-text available
Accumulation of reserve compounds (i.e., lipids and chrysolaminarin) in diatoms depends on the environmental conditions, and is often triggered by stress conditions, such as nutrient limitation. Manipulation of CO2 supply can also be used to improve both lipids and carbohydrates accumulation. Given the high diversity among diatoms, we studied the t...
Article
The removal of intact chloroplasts from their cell wall confinement offers a novel way to obtain lipophilic nutrients from green biomass, especially carotenoids and galactolipids. These latter are the main membrane lipids in plants and they represent a major source of the essential α-linolenic acid (18:3; ALA). Nevertheless, knowledge on their dige...
Article
Milk fat globule membrane conditions the reactivity and enzymatic susceptibility of milk lipids. The use of bovine membrane extracts to make infant formulas more biomimetic of human milk has been suggested recently. A comparison of the physico-chemical behavior of human and bovine milk membrane extracts and their interaction with gastric lipase is...
Article
Secreted phospholipases A2 (sPLA2) are water-soluble lipolytic enzymes that act at the interface of organized lipid substrates, where the catalytic step is coupled to various interfacial phenomena as enzyme penetration, solubilisation of reaction products, lateral packing and loss of mechanical stability of organized assemblies of phospholipid mole...
Article
Dietary fats are present in the diet under different types of structures, such as spread vs emulsions (notably in processed foods and enteral formula), and interest is growing regarding their digestion and intestinal absorption. In clinical trials, there is often a need to add stable isotope-labeled triacylglycerols (TAGs) as tracers to the ingeste...
Article
Full-text available
The inhibition of recombinant CpLIP2 lipase/acyltransferase from Candida parapsiolosis was considered a key model for novel antifungal drug discovery and a potential therapeutic target for candidiasis. Lipases have identified recently as potent virulence factors in C. parapsilosis and some other yeasts. The inhibition effects of orlistat and four f...
Article
Intrinsically disordered proteins (IDPs) are proteins that provide many functional advantages in a large number of metabolic and signalling pathways. Because of their high flexibility that endows them with pressure-, heat- and acid-resistance, IDPs are valuable metabolic regulators that help algae to cope with extreme conditions of pH, temperature,...
Data
Supplementary Data of the paper in Biochimie by Amal SALHI et al. (DOI: 10.1016/j.biochi.2019.07.004)
Article
Porcine pancreatic extracts (PPE), also named pancreatin, are commonly used as a global source of pancreatic enzymes for enzyme replacement therapy in patients with exocrine pancreatic insufficiency. They are considered as a good substitute of human pancreatic enzymes and they have become a material of choice for in vitro models of digestion. Never...
Poster
Full-text available
We have developed a new instrument to measure simultaneously the interfacial tension and interfacial dilatational rheology of two Bubbles or Drops immersed in the same liquid. It is possible to put it in contact and characterize the interaction between these Bubbles and/or Droplets. The device used is an implementation of the original Tracker devic...
Article
Microalgae can produce large quantities of triacylglycerols (TAGs) and other neutral lipids that are suitable for making biofuels and as feedstocks for green chemistry. However, TAGs accumulate under stress conditions that also stop growth, leading to a trade-off between biomass production and TAG yield. Recently, in the model marine diatom Phaeoda...
Article
Full-text available
Correction for ‘Free fatty acid release from vegetable and bovine milk fat-based infant formulas and human milk during two-phase in vitro digestion’ by Jeske H. J. Hageman et al. , Food Funct. , 2019, 10 , 2102–2113.
Article
Background: Bovine milk fat is increasingly used in infant formula (IF). The triacylglycerol (TAG) structure of bovine milk fat might be beneficial for digestion and absorption. We investigated the release of fatty acids (FAs) of IF containing different fat blends and compared this to human milk. Methods: Fresh human milk was sampled and two IFs we...
Article
Full-text available
Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of...
Article
The lipids and some surfactants present in oral lipid-based drug delivery systems are potential substrates for the various lipases involved in gastrointestinal (GI) lipolysis. The levels of these enzymes, together with pH and biliairy secretion, are important parameters that condition the fate of lipid-based formulations (LBF) and the dispersion, s...
Presentation
Full-text available
Collection of a few slides for introducing lipase research at Marseille, including milestones
Article
Bioactive compounds identification and isolation from natural complex samples, even though being a difficult task, is of great interest in the drug discovery field. We describe here an innovative strategy for the identification of a new gastric lipase inhibitor from star anise for the treatment of obesity. After plant screening assays for gastric l...
Article
Rabbit gastric extracts (RGE) is a source of gastric enzymes for in vitro digestion studies. While its gastric lipase activity has been characterized and compared to other lipases, its pepsin activity has not been studied. We measured pepsin activity in RGE using both hemoglobin and azocoll a substrates, and identified the protein separated by SDS-...
Article
Full-text available
Bile acid (BA) secretion and circulation in chronic pancreatitis (CP) patients with exocrine pancreatic insufficiency (EPI) were investigated by measuring simultaneously postprandial levels of individual BAs in duodenal contents and blood plasma using LC-MS/MS. CP patients and healthy volunteers (HV) were intubated with gastric and duodenal tubes p...
Article
Résumé La composition chimique des formules infantiles a été optimisée, mais pas leur structure qui reste souvent basée sur des gouttelettes lipidiques submicroniques (0,5 μm) avec des interfaces néoformées, différent des globules gras laitiers (4 μm). Or, il a été récemment démontré que la structure des lipides dans le lait maternel module les cin...
Article
Full-text available
Talaromyces thermophilus lipase (TTL) was found to hydrolyze monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) substrates presented in various forms to the enzyme. Different assay techniques were used for each substrate: pHstat with dioctanoyl galactolipid-bile salt mixed micelles, barostat with dilauroyl galactolipid mono...
Article
Full-text available
Food effects on oral drug bioavailability can have significant impact on the provision of safe and reliable oral pharmacotherapy. A mechanistic understanding of the events that contribute to the occurrence of food effects is therefore critical. An increased oral bioavailability is often seen for poorly water-soluble drugs after co-administration wi...
Article
Background: Heterodimeric phospholipase A2from venom glands of Tunisian scorpion Scorpio maurus (Sm-PLGV) had been purified. It contains long and short chains linked by a disulfide bridge. Sm-PLGV exhibits hemolytic activity towards human erythrocytes and interacts with phospholipid monolayers at high surface pressure. The investigation of structu...
Article
Intrinsically disordered proteins (IDPs) lack stable secondary and tertiary structure under physiological conditions in the absence of their biological partners and thus exist as dynamic ensembles of interconverting conformers, often highly soluble in water. However, in some cases, IDPs such as the ones involved in neurodegenerative diseases can fo...
Article
Usual methods for the continuous assay of lipolytic enzyme activities are mainly based on the titration of free fatty acids, surface pressure monitoring or spectrophotometry using substrates labeled with specific probes. These approaches only give a partial information on the chemistry of the lipolysis reaction and additional end-point analyses are...
Article
The interaction of pancreatic lipase-related protein 2 (PLRP2) with various micelles containing phospholipids was investigated using pHstat enzyme activity measurements, differential light scattering, size exclusion chromatography (SEC) and transmission IR spectroscopy. Various micelles of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and lyso...
Method
Full-text available
Assay of pancreatic lipase activity in pancreatin using the pHstat technique and tributyrin as substrate
Article
The target of rapamycin (TOR) signaling pathway regulates fundamental intracellular functions critical for cell viability and proliferation. Manipulation of TOR in high lipid-producing microalgae may help overcome the trade-off between biomass production and lipid yield that still impairs the viable production of biofuel from microalgae. In this st...
Article
Introduction et but de l’étude Assurer une croissance et un développement optimal chez les nouveau-nés prématurés est une priorité (OMS, 2015). Contrairement aux formules infantiles, le lait maternel permet de limiter les entérocolites nécrosantes, mais doit être supplémenté pour répondre aux besoins nutritionnels du prématuré. Cette stratégie est...
Presentation
Full-text available
Guinea pig pancreatic lipase-related protein type 2 (GPLRP2) hydrolyzes a large set of lipid substrates, but displays however some selectivity depending on the supramolecular structure of substrate and the presence of surfactants like bile salts (NaTDC). We used Fourier transform infrared (FTIR) spectroscopy to study the interactions between phosph...
Presentation
Full-text available
Oil-in-water emulsions are common vehicles for lipids as nutrients and for the delivery of poorly water-soluble drugs. Enhancing oral bioavailability of these drugs using lipid-based formulations (LBF) or self-emulsifying drug delivery systems is one of the current challenges in pharmaceutical industry. Many of the compounds found in LBF (acylglyce...
Article
Full-text available
Novel microbial phospholipases A (PLAs) can be found in actinomycetes which have been poorly explored as producers of this activity. To investigate microbial PLA production, efficient methods are necessary such as high-throughput screening (HTS) assays for direct search of PLAs in microbial cultures and cultivation conditions to promote this activi...
Data
Detection of phospholipase activity using the agar-plate method with enriched media containing egg yolk PC and rhodamine 6G as indicator exposed at UV light (254 nm) Pichia pastoris strains cultivated after 4 days at 30 °C in which (A) P. pastoris wild-type strain (X-33) without gene insertion, and (B) P. pastoris expressing the enzyme GPLRP2 which...
Data
Kinetics of activity obtained for A99 strain extracts produced by liquid (SmF) and solid state fermentation (SSF) after 10 days at 30 °C Samples were diluted 2 and 5 fold for SmF and SFF extracts, respectively; 20 uL of diluted sample were added to hydrolysis reaction using PC as a substrate through the cHTS-PLA method.
Data
Analysis by TLC of PC hydrolysis by PLAs produced by SSF of selected strains of each Collection Lane 1: non-hydrolyzed PC substrate; Lane 2: PC hydrolyzed by A99 strain extract (Collection 3); Lane 3: PC hydrolyzed by AC3-11 strain extract (Collection 2); Lane 4: PC hydrolyzed by 6C-3 strain extract (Collection 1). The accumulation of Lyso-PC was t...
Data
Analysis by TLC of EEPC hydrolysis revealed through UV light (254 nm) Lane 1, non hydrolyzed EEPC; lane 2, EEPC hydrolyzed by ppPLA_2; lane 3, EEPC hydrolyzed by 6C-3 strain crude extract produced by SSF; lane 4, purified a-eleostearic acid. The mobile phase employed was chloroform/methanol/acetic acid/NaCl 150 mM, 50:25:8:4 (v/v/v/v) containing 0....
Data
Solubility test of egg PC (≈ 60%) with several alcohols (A): egg PC alone; (B): egg PC + ethanol; (C): egg PC + 2-methyl-2-butanol; (D): egg PC +2-methyl-2-propanol; (E): egg PC + 2-propanol. Alcohols were added to egg PC and mixed by vortex during 1.5 min at 25 °C to have a final concentration of 120 mg/mL. In B, egg PC had better solubility but a...
Article
The cDNA encoding human gastric lipase (HGL) was integrated into the genome of Pichia pastoris using the pGAPZα A transfer vector. The HGL signal peptide was replaced by the yeast α-factor to achieve an efficient secretion. Active rHGL was produced by the transformed yeast but its levels and stability were dependent on the pH. The highest activity...