Fred Lisdat

• phd 1992, habilitation 2004
• Chair at Technical University of Applied Sciences Wildau

In this study, photobioelectrodes based on a ferredoxin-modified photosystem I (PSI-Fd) from Thermosynechococcus vestitus have been prepared and characterized regarding the direct electron transfer between PSI-Fd and the electrode. The modified PSI with the covalently linked ferredoxin (Fd) on its stromal side has been immobilized on indium-tin-oxi...

The present study investigates basic features of a photoelectrochemical system based on CeO2 nanoparticles fixed on gold electrodes. Since photocurrent generation is limited to the absorption range of the CeO2 in the UV range, the combination with metal nanoparticles has been studied. It can be shown that the combination of silver nanoparticles wit...

Synthetic materials can be combined with biological components in many ways. One example that provides scientists with multiple challenges is a photobioelectrode that converts sunlight into electrons in a biohybrid approach. In the present study several key parameters are evaluated concerning their influence on the direct electron transfer from a 3...

We describe the development of biohybrid electrodes constructed via combination of electrospun (e-spun) 3D indium tin oxide (ITO) with the trimeric supercomplex photosystem I and the small electrochemically active protein cytochrome c (cyt c). The developed 3D surface of ITO was created by electrospinning of a mixture of polyelthylene oxide (PEO) a...

Parkinson’s disease is one of the most frequent diseases of the central nervous system with rather severe consequences for patients. Since the disorder can not be cured, the dopamine level in the brain needs to adjusted. Here several key enzymes of the dopamine metabolism [e. g. catechol-O-methyl transferase (COMT)] are targets for administered dru...

The integration of photoactive proteins such as photosystem I and II in electrode architectures has gained much attention during the last years for light-to-current and light-to-chemical conversion [1,2]. Here, we have combined the photophysical properties of the biological photosystem II (PSII) with a second artificial, light-sensitive entity (PbS...

During the last decade photoelectrochemical devices which couple biochemical reactions to light-sensitive electrodes have gained increasing research interest. These kind of light-triggered systems have the inherent advantage that an additional tool - the light - can be used to control the reactions at a sensing electrode. This allows a light-direct...

Introduction Quantum dots (QDs) have become attractive tools in bioanalysis because of their advantageous optical and electrochemical properties. This relates to applications exploiting fluorescence, electrochemiluminescence or photocurrent generation [1]. For the latter case QDs are immobilized on conductive electrodes. By light excitation charge...

Photobioelectrodes represent one of the examples where artificial materials are combined with biological entities to undertake semi-artificial photosynthesis. Here, an approach is described that uses reduced graphene oxide (rGO) as an electrode material. This classical 2D material is used to construct a three-dimensional structure by a template-bas...

In this report we combine the catalytic properties of CeO2 nanoparticles with their transduction ability for photoelectrochemical sensing. This study highlights the usage of CeO2 providing catalytic activity towards H2O2,...

The enzyme monoamine oxidase B (Mao B), which is involved in dopamine metabolism, is an important drug target in Parkinson’s disease (PD). The medical treatment with several Mao B inhibitors is well established but only little personalized since the monitoring of the patients Mao B activity is complex and requires sophisticated laboratory equipment...

A photobioelectrochemical tandem cell is presented in which two photoelectrodes have been functionally coupled with two biocatalysts for supplying the light‐driven reaction with charge carriers from glucose conversion. The cell allows the generation of electricity from biofuels and light with a high open‐circuit voltage of 1 V. Abstract We report...

We report on a photobioelectrochemical fuel cell consisting of a glucose‐oxidase‐modified BiFeO3 photobiocathode and a quantum‐dot‐sensitized inverse opal TiO2 photobioanode linked to FAD glucose dehydrogenase via a redox polymer. Both photobioelectrodes are driven by enzymatic glucose conversion. Whereas the photobioanode can collect electrons fro...

The coupling of biological entities with electrodes has already quite some history and has reached a status which is not only based on phenomenological descriptions. Nowadays, we are able to effectively couple redox centres within protein molecules to electrochemical transducers. This allows the transduction of a biochemical reaction into an electr...

Trimeric photosystem I from the cyanobacterium Thermosynechococcus elongatus (TePSI) is an intrinsic membrane protein, which converts solar energy into electrical energy by oxidizing the soluble redox mediator cytochrome c6 (Cyt c6) and reducing ferredoxin. Here, we use cryo-electron microscopy and small angle neutron scattering (SANS) to character...

Introduction Quantum dots (QDs) have become attractive tools in bioanalysis because of their advantageous optical and electrochemical properties. This relates to applications exploiting fluorescence, electrochemiluminescence or photocurrent generation [1]. For the latter case QDs are immobilized on conductive electrodes. By light excitation charge...

During the last decade photoelectrochemical devices which couple biochemical reactions to light-sensitive electrodes have gained increasing research interest. These kind of light-triggered systems have the inherent advantage that an additional tool - the light - can be used to control the reactions at a sensing electrode. This allows a light-direct...

Parkinson’s disease is one of the most frequent diseases of the central nervous system with rather severe consequences for patients. Since the disorder can not be cured, the dopamine level in the brain needs to adjusted. Here several key enzymes of the dopamine metabolism [e. g. catechol-O-methyl transferase (COMT)] are targets for administered dru...

A light-controlled multiplexing platform has been developed on the basis of a quantum dot-sensitized inverse opal TiO 2 electrode with integrated biocatalytic reactions. Spatially resolved illumination enables multiplexed sensing and imaging...

For the treatment of Parkinson’s disease as one of the most frequent diseases of the central nervous system several key enzymes of the dopamine metabolism, e.g. catechol‐O‐methyl transferase (COMT), are drug targets. For an efficient and long‐lasting treatment the activity of this enzyme should be monitored. In this study an electrochemical approac...

In recent years the use of photoelectrodes based on conductive metal oxides has become very popular in the field of photovoltaics. Application of 3D electrodes is holding great promises since they can integrate large amounts of photoactive proteins. In this study photosystem I (PSI) from thermophilic cyanobacterium Thermosynechococcus elongatus has...

Layer-by-layer (LbL) assembly is a widely used tool for engineering materials and coatings. In this Perspective, dedicated to the memory of ACS Nano associate editor Prof. Dr. Helmuth Möhwald, we discuss the developments and applications that are to come in LbL assembly, focusing on coatings, bulk materials, membranes, nanocomposites, and delivery...

Triggering electrochemical reactions with light provides a powerful tool for the control of complex reaction schemes on photoactive electrodes. Here, we report on the light-directed, multiplexed detection of enzymatic substrates using a non-structured gold electrode modified with CdSe/ZnS quantum dots (QDs) and two enzymes, i.e. glucose oxidase (GO...

For successful production of photobioelectrodes two major aspects have to be addressed: The proper orientation of the photo-active biomolecules on the electrode surface and a good electrical connection with the electrode. In this work a step-by-step procedure for the successful construction of a photosystem I (PSI)-based photobioelectrode is shown....

Die funktionale Kopplung von photoaktiven Nanostrukturen mit Enzymen stellt eine neue Strategie zum Aufbau lichtgesteuerter biohybrider Systeme dar. Hier sind Untersuchungen zusammengefasst, welche die effiziente Kontaktierung der FAD-abhängigen Glukosedehydrogenase (FAD-GDH) mit Hilfe eines Osmium-Redoxpolymers (P Os ) an PbS-Quantum Dots (PbS QDs...

A biohybrid photobioanode mimicking the Z‐scheme has been developed by functional integration of photosystem II (PSII) and PbS quantum dots (QDs) within an inverse opal TiO2 architecture giving rise to a rather negative water oxidation potential of about −0.55 V vs. Ag/AgCl, 1 m KCl at neutral pH. The electrical linkage between both light‐sensitive...

The functional coupling of photo-active nanostructures with enzymes creates a strategy for the design of light-triggered biocatalysts. This study highlights the efficient wiring of FAD-dependent glucose dehydrogenase (FAD-GDH) to PbS QD-sensitized inverse opal TiO2 electrodes (IO-TiO2) by means of an Os-complex-containing redox polymer for the ligh...

The binding of photosystem I (PS I) from Thermosynechococcus elongatus to the native cytochrome (cyt) c6 and cyt c from horse heart (cyt cHH), is analyzed by oxygen consumption measurements, isothermal titration calorimetry (ITC), and rigid body docking combined with electrostatic computations of binding energies. While PS I has a higher affinity f...

Abasic sites can occur in DNA for different reasons, and thus the detection of this special molecular structure has turned into the focus of research. Here we have investigated the hybridization of abasic ssDNA to immobilized ssDNA probes by impedance spectroscopy. For this purpose three different abasic 25mer ssDNA (abasic site near the electrode;...

In this study, polythiophene copolymers have been used as modifier for electrode surfaces in order to allow the immobilization of active pyrroloquinoline quinone dependent glucose dehydrogenase (PQQ-GDH) and to simultaneously improve the direct electrical connection of the enzyme with the electrode. Polymer films are electrosynthesized in aqueous s...

Die Kombination von fortschrittlichen Materialien und kontrolliertem Oberflächendesign mit komplexen Proteinen aus der natürlichen Photosynthese ist derzeit eines der Hauptthemen bei der Entwicklung von Biohybridsystemen und Biophotovoltaik. In dieser Studie werden transparente makroporöse Indium-Zinn-Oxid-(μITO-) Elektroden mit dem trimeren Superk...

Primary aldosteronsim (PA) is the most frequent cause of secondary hypertension. [...]

Inspired by natural photosynthesis, coupling of artificial light-sensitive entities with biocatalysts in a biohybrid format can result in advanced photobioelectronic systems. Herein, we report on the integration of sulfonated polyanilines (PMSA1) and PQQ glucose dehydrogenase (PQQ-GDH) into inverse opal TiO2 (IO-TiO2) electrodes. While PMSA1 introd...

Dopamine and other catecholamines play a keyrole for the interaction of numerous processes in the neuronal network. Hence, numerous detection methods have been developed. Electrochemical sensor developments for catecholamines are characterized mostly by complex modification strategies in order to avoid a disturbance of the sensor signal by interfer...

The chapter introduces the use of impedance spectroscopy for biosensing. It gives an overview on biocatalytic and bioaffinity-based systems. Special attention is devoted to the detection of DNA and DNA-binding molecules.

Artificial light-driven signal chains are particularly important for the development of systems converting light into a current, into chemicals or for light-induced sensing. Here, we report on the construction of an all-protein, light-triggered, catalytic circuit based on photosystem I, cytochrome c (cyt c) and human sulfite oxidase (hSOX). The def...

The reproducible detection of neurotransmitters such as serotonin in the presence of interfering substances is still a topic in analytical sciences. In the present study, a 3D mesoporous ITO electrode (μITO) has been used for the determination of serotonin in the presence of high concentrations 5-hydroxyindoleacetic acid (5-HIAA) as an alternative...

The establishment of a polythiophene-supported electron transfer of PQQ-dependent glucose dehydrogenase (PQQ-GDH) at multiwalled carbon nanotubes is reported. For this purpose, thiol-functionalized MWCNTs are deposited on a gold electrode, which is further modified by on-top electropolymerization of different thiophene monomers. The enzyme is coval...

In this work, we report on the successful assembly of cyanobacterial photosystem I (PSI) on carbon nanotubes for light-to-current conversion applications. For this purpose, glassy carbon electrodes (GCE) have been modified with multi-walled carbon nanotubes (MWCNTs). The surface of the MWCNTs has been adjusted in a non-invasive way by the use of a...

The combination of the biocatalytic features of enzymes with the unique physical properties of nanoparticles in a biohybrid system provides a promising approach for the development of advanced bioelectrocatalytic devices. This study describes the construction of photoelectrochemical signal chains based on CdSe/ZnS quantum dot (QD) modified gold ele...

This study introduces a thermally responsive, polymer-based electrode system. The key component is a surface-attached, temperature-responsive poly(oligoethylene glycol) methacrylate (poly(OEGMA)) type polymer bearing photoreactive benzophenone and carboxy groups containing side chains. The responsive behavior of the polymer in aqueous media has bee...

Die hier durchgeführten Untersuchungen ermöglichen ein besseres Verständnis der Elektronentransferprozesse zwischen der Flavin-abhängigen Fruktosedehydrogenase (FDH) und dem Redoxprotein Cytochrom c (Cyt c). Dies liefert im Hinblick auf sensorische Anwendungen wichtige Erkenntnisse für vorteilhafte Sensorarchitekturen und deren Messbedingungen. Es...

The redox behavior of proteins plays a crucial part in the design of bioelectronic systems. We have demonstrated several functional systems exploiting the electron exchange properties of the redox protein cytochrome c (cyt c) in combination with enzymes and photoactive proteins. The operation is based on an effective reaction at modified electrodes...

The performance of an enzymatic biofuel cell in physiological media depends not only on the electrode architecture but is also influenced by substances in these media which can be directly oxidized or reduced at the anode and/or cathode or have an impact on the biocatalytic processes. For the anode construction carbon nanotubes are modified with a...

The combination of advanced materials and defined surface design with complex proteins from natural photosynthesis is currently one of the major topics in the development of biohybrid systems and biophotovoltaic devices. In this study transparent mesoporous indium tin oxide (µITO) electrodes have been used in combination with the trimeric supercomp...

We report on the pH-dependent bioelectrocatalytic activity of the redox enzyme xanthine dehydrogenase (XDH) in the presence of sulfonated polyaniline PMSA1 (poly(2-methoxyaniline-5-sulfonic acid)-co-aniline). UV-vis spectroscopic measurements with both components in solution reveal electron transfer from the hypoxanthine (HX) reduced enzyme to the...

The creation of artificial electron transfer (ET) chains based on the defined arrangement of enzymes and redox proteins on electrode surfaces represents an interesting field in bioelectronics ¹ . One approach exploits the self exchange properties of cytochrome c (cyt c ) to build up new sensing electrodes ² . In this study we have investigated the...

Cytochrome c (cyt c , 13 kDa) is a well-characterized redox protein[1] which has been used extensively as model protein and for the construction of artificial signal chains in biosensors.[2,3] Often this involves immobilization of the cationic cyt c on gold electrodes modified by self-assembled monolayers (SAM) of packed anionic alkanethiols.[4] On...

Hybrid systems using the unique features of natural photosynthesis are increasingly in the focus of current research. Particularly the two protein complexes photosystem I and II (PS) of the oxygenic photosynthesis have attracted the attention of researchers to build up new solar energy-converting systems. In such biohybrid systems an efficient coup...

The creation of electron transfer (ET) chains based on the defined arrangement of enzymes and redox proteins on electrode surfaces represents an interesting approach within the field of bio-electrocatalysis. In this study, we investigated the ET reaction of the flavin-dependent enzyme fructose dehydrogenase (FDH) with the redox protein cytochrome c...

The engineering of renewable and sustainable protein-based light-to-energy converting systems is an emerging field of research. Here, we report on the development of supramolecular light-harvesting electrodes, consisting of the redox protein cytochrome c working as a molecular scaffold as well as a conductive wiring network and photosystem I as a p...

Es wurde ein dreidimensionales Polymerfasernetzwerk aufgebaut, charakterisiert und anschließend daran das Enzym Pyrrolochinolinchinon-abhängige Glukosedehydrogenase (PQQ)GDH gebunden. Das Polymerfasernetzwerk wurde durch Elektrospinnen einer Mischung des Polymers Polyacrylnitril und verschiedener leitfähiger Polymere der Polyanilin-Familie auf Indi...

Orthopoxvirus species like cowpox, vaccinia and monkeypox virus cause zoonotic infections in humans worldwide. Infections often occur in rural areas lacking proper diagnostic infrastructure as exemplified by monkeypox, which is endemic in Western and Central Africa. While PCR detection requires demanding equipment and is restricted to genome detect...

Electrospinning is known as a fabrication technique for electrode architectures that serve as immobilization matrices for biomolecules. The current work demonstrates a novel approach to construct a conductive polymeric platform, capable not only of immobilization, but also of electrical connection of the biomolecule with the electrode. It is produc...

The fast and simple detection of increased protein concentrations in cerebrospinal liquids is preferable in the emergency medicine and it can help to avoid unnecessary laboratory work by an early classification of neurological diseases. Here a test system is developed which is based on the electrostatic interaction between negatively charged gold n...

Ricin is one of the most toxic plant toxins known. Its accessibility and relative ease of preparation makes it a potential agent for criminal or bio-terrorist attacks. Detection of ricin from unknown samples requires differentiation of ricin from the highly homologous Ricinus communis agglutinin which is currently not feasible using immunological m...

A photo-electrochemical sensor for the specific detection of guanosine monophosphate (GMP) is demonstrated, based on three enzymes combined in a coupled reaction assay. The first reaction involves the adenosine triphosphate (ATP)-dependent conversion of GMP to guanosine diphosphate (GDP) by guanylate kinase, which warrants substrate specificity. Th...

One major vital element of the oxygenic photosynthesis is Photosystem I (PSI). We report on the construction of graphene-based nanophotohybrid light-harvesting architectures consisting of PSI supercomplexes adsorbed onto π-system-modified graphene interfaces. The light-driven nanophotobioelectrochemical architectures have been designed on a modifie...

Biochemical signal chains and pathways are characterized by an effective coupling of individual reaction steps resulting in a rather high specificity in signal transduction. Additionally such highly complex systems allow a switching between different reaction cascades, depending on the presence of a certain molecule. Apparently, these efficient bio...

Polymers can provide a suitable chemical environment for the immobilization of biomolecules in an active form on surfaces. Furthermore they can be used to wire redox proteins and enzymes with electrodes. For this purpose mainly redox polymers with well-defined redox centres and conductive polymers with intrinsic electron conductivity are applied. P...

Artificial systems exploiting the features of natural photosynthesis are increasingly becoming a focus of current research. Particularly the two photosystems (PS) of the oxygenic photosynthesis have attracted the attention of researchers to build up new solar energy-converting systems. [1,2] Besides the light-to-current conversion, PSI may also be...

The creation of analytical electron transfer (ET) chains based on the defined arrangement of enzymes and the redox protein cytochrome c (cyt c ) on electrode surfaces represents an emerging field in bioelectronics. 1,2 In this study we investigate the ET reaction of the flavin-dependent enzyme fructose dehydrogenase (FDH) with the redox protein cyt...

Carbon nanotubes (CNTs) arranged in 3 dimensional structures represent an interesting material for the development of biocatalytic electrodes. Due to their architecture they can provide docking places for enzymes and after modification of the surface properties often a direct electrochemistry can be observed. The direct electron transfer where the...

Artificial approaches to mimick natural photosynthesis are an emerging field of research with impact on photobiocatalytic systems and photobiovoltaic applications. Herein, we manage the assembly of two heterologous proteins exhibiting key features for the usage in biohybrid light-converting electrode systems. We combine the photon converting pigmen...

Photosystem I (PSI) is a key component of the oxygenic photosynthetic electron transport chain because of its light-induced charge separation and electron transfer (ET) capabilities. We report the fabrication of an efficient graphene-biohybrid light-harvesting electrode consisting of cyanobacterial trimeric PSI complexes immobilized onto Π-system-m...

The assembly of redox proteins on electrodes is an important step in biosensor development. Recently, p-sulfonato-calix[4]arene was shown to act as "molecular glue" for the assembly and crystallization of cytochrome c (cyt c). Electrochemical data are presented for microscale cyt c-calixarene crystals grown on self-assembled monolayers (SAM)-modifi...

Die Assemblierung von Redoxproteinen auf Elektrodenoberflächen ist ein entscheidender Schritt für die Entwicklung von biohybriden Systemen. Kürzlich konnte gezeigt werden, dass p-sulfoniertes Calix[4]aren als “molekularer Klebstoff” für die Assemblierung und Kristallbildung von Cytochrom c (Cyt c) dient. Hier präsentieren wir die ersten elektrochem...

Diese Studie beschreibt einen labelfreien impedimetrischen Sensor auf der Grundlage von kurzen einzelsträngigen DNA-Erkennungselementen für den Nachweis von Hybridisierungsereignissen. Der Fokus der Arbeit liegt auf der Aufklärung des Einflusses der Ziel-DNA-Länge und der Erkennungssequenzposition auf die sensorische Leistungsfähigkeit. Die impedim...

This study reports on the investigation of a thermo­responsive polymer as a thin film on electrodes and the influence of coupling a peptide and an antibody to the film. The utilized polymer from the class of poly(oligoethylene glycol)-methacrylate polymers (poly(OEGMA)) with carboxy functions containing side chains was synthesized and properly char...

We report on efficient bioelectrocatalysis of the redox enzyme fructose dehydrogenase (FDH) upon its interaction with a sulfonated polyaniline PMSA1 [poly(2-methoxyaniline-5-sulfonic acid)-co-aniline]. This interaction has been monitored in solution and on the surface of planar and macroporous indium tin oxide (ITO) electrodes by UV-vis and cyclic...

Polymer-multiwalled carbon nanotube (MWCNT) nanohybrids, which differ in surface charge have been synthesized to study the bioelectrocatalysis of adsorbed cellobiose dehydrogenase (CDH) from Phanerochaete sordida on gold electrodes. To obtain negatively charged nanohybrids, poly(3-amino-4-methoxybenzoic acid-co-aniline) (P(AMB-A)) was covalently li...

Sulfonierte Polyaniline erwiesen sich bereits als geeignete Polymere für den Aufbau von Biosensoren. Aus diesem Grund setzten wir unterschiedlich substituierte Polymerformen für die Untersuchungen der direkten Elektronenübertragung zum Redoxenzym PQQ-GDH (Pyrrolochinolinchinon-abhängige Glukosedehydrogenase) ein. Dafür wurden zuerst neue Copolymere...

Artificial assemblies consisting of the cationic cytochrome c (cyt c) and double-stranded DNA are interesting for the field of biohybrid systems because of the high electro-activity of the incorporated redox protein. However, little is known about the interactions between these two biomolecules. Here, the complex of reduced cyt c and a 41 base pair...

We report on the fabrication of complex electrode architectures for efficient direct bioelectrocatalysis. In the developed procedure, the redox enzyme pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) entrapped in a sulfo-natedpolyaniline [poly(2-methoxyaniline-5-sulfonic acid)-co-aniline; PMSA1] was immobilized on macroporous indi...

This study describes a label-free impedimetric sensor based on short ssDNA recognition elements for the detection of hybridization events. We concentrate on the elucidation of the influence of target length and recognition sequence position on the sensorial performance. The impedimetric measurements are performed in the presence of the redox system...

Conversion of light into an electrical current based on biohybrid systems mimicking natural photosynthesis is becoming increasingly popular. Photosystem I (PSI) is particularly useful in such photo-bioelectrochemical devices. Herein, we report on a novel biomimetic approach for an effective assembly of photosystem I with the electron transfer carri...

Titel: " Advanced unidirectional photocurrent generation via cytochrome c as reaction partner for directed assembly of photosystem I " Kai R. Stieger, Sven. C. Feifel, Heiko Lokstein and Fred Lisdat (I) Assembly Study of photosystem I on cytochrome c modified electrodes 1A) Dependence of protein deposition on pH Assembly studies have been performed...

A supramolecular multicomponent protein architecture on electrodes is developed that allows the establishment of bidirectional electron transfer cascades based on interprotein electron exchange. The architecture is formed by embedding two different enzymes (laccase and cellobiose dehydrogenase) and a redox protein (cytochrome c) by means of carboxy...

Aus drei Proteinkomponenten wird eine supramolekulare Proteinarchitektur auf Elektroden aufgebaut. Damit kann eine bidirektionale Elektronentransferkaskade infolge intermolekularen Elektronentransfers etabliert werden. Der supramolekulare Aufbau im Multischichtformat wird durch das Einbetten zweier unterschiedlicher Enzyme (Cellobiosedehydrogenase...

Dopant-functionalized anilines with improved electrocatalytic properties are promising building blocks for the construction of bioelectronic devices [1]. The present study is devoted to the use of polyanillines possessing different substitution patterns in the interaction with the enzyme PQQ-GDH (pyrroloquinoline quinone dependent glucose dehydroge...

Fully electro-active multilayer architectures combining the redox protein cytochrome c and the enzyme laccase by the use of silica nanoparticles as artificial matrix have been constructed on gold electrodes capable of direct dioxygen reduction. Laccase form trametes versicolor and cytochrome c from horse heart were electrostatically co-immobilized...

A feasible approach to construct multilayer films of sulfonated polyanilines – PMSA1 and PABMSA1 – containing different ratios of aniline, 2-methoxyaniline-5-sulfonic acid (MAS) and 3-aminobenzoic acid (AB), with the entrapped redox enzyme pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) on Au and ITO electrode surfaces, is descri...