Frank Gaupels

Frank Gaupels
Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) | HZM · Institute of Biochemical Plant Pathology

PD Dr.

About

46
Publications
8,095
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1,451
Citations
Citations since 2016
19 Research Items
853 Citations
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2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
Introduction
Frank Gaupels currently works at the Institute of Biochemical Plant Pathology, Helmholtz Zentrum München. Frank does research in Phytochemistry, Biochemistry and Plant Protection. His current project is 'Nitric oxide signalling in plant stress responses.'
Additional affiliations
July 2015 - present
Justus-Liebig-Universität Gießen
Position
  • Privatdozent
January 2010 - present
January 2007 - December 2009
Università degli studi di Verona
Education
November 2014 - July 2015
Justus-Liebig-Universität Gießen
Field of study
  • Plant physiology and phytopathology
January 2002 - January 2006

Publications

Publications (46)
Article
Full-text available
High doses of ozone (O 3 ) and nitrogen dioxide (NO 2 ) cause damage and cell death in plants. These two gases are among the most harmful air pollutants for ecosystems and therefore it is important to understand how plant resistance or sensitivity to these gases work at the molecular level and its genetic control. We compared transcriptome data fro...
Article
Full-text available
Background: Nitrogen dioxide (NO2) triggers hypersensitive response (HR)-like cell death in Arabidopsis thaliana. A high-throughput mutant screen was established to identify genes involved in this type of programmed cell death. Results: Altogether 14,282 lines of SALK T-DNA insertion mutants were screened. Growing 1000 pooled mutant lines per tr...
Article
Full-text available
Some of the salicylic acid-deficient Arabidopsis eds5 mutants have an unnoticed fah1-2 background mutation, which could cause salicylic acid- and EDS5-independent mutant phenotypes.
Article
Full-text available
Nitrogen dioxide (NO2) forms in plants under stress conditions, but little is known about its physiological functions. Here, we explored the physiological functions of NO2 in plant cells using short-term fumigation of Arabidopsis (Arabidopsis thaliana) for 1 h with 10 parts per million (ppm) NO2. Although leaf symptoms were absent, the expression o...
Thesis
Full-text available
Signalling during induced defence responses of plants to biotic and abiotic stresses is often multi-layered and rather complex. By developing signal networks the plant maintains high flexibility, which is necessary for adequate reactions to ever-changing environmental conditions and a multitude of different pathogens. Redox signalling is a good exa...
Article
Full-text available
Rapid long-distance signalling is an emerging topic in plant research, and is particularly associated with responses to biotic and abiotic stress. Systemic acquired resistance (SAR) to pathogen attack is dependent on nitric oxide (NO) and reactive oxygen species (ROS) such as hydrogen peroxide (H2 O2 ). By comparison, systemic wound responses (SWRs...
Article
Full-text available
This study aimed to understand the molecular mechanisms of nitrogen dioxide (NO2)-induced toxicity and cell death in plants. Exposure of Arabidopsis to high concentrations of NO2 induced cell death in a dose-dependent manner. No leaf symptoms were visible after fumigation for 1 h with 10 parts per million (ppm) NO2 However, 20 ppm NO2 caused necrot...
Article
Full-text available
Nitric oxide is an important signalling molecule which is involved in many different physiological processes in plants. Here we report about a NO-fixing mechanism in Arabidopsis, which allows the fixation of atmospheric NO into nitrogen metabolism. We fumigated Arabidopsis plants cultivated in soil or as hydroponic cultures during the whole growing...
Article
Full-text available
Cucurbits developed the unique extrafascicular phloem (EFP) as a defensive structure against herbivorous animals. Mechanical leaf injury was previously shown to induce a systemic wound response in the EFP of pumpkin (Cucurbita maxima). Here, we demonstrate that the phloem antioxidant system and protein modifications by NO are strongly regulated dur...
Article
Full-text available
Despite the importance of superoxide dismutases (SODs) in the plant antioxidant defence system little is known about their regulation by post-translational modifications. Here, we investigated the in vitro effects of nitric oxide derivatives on the seven SOD isoforms of Arabidopsis thaliana. S-nitrosoglutathione, which causes S-nitrosylation of cys...
Article
Full-text available
In plant cells the free radical nitric oxide (NO) interacts both with anti- as well as prooxidants. This review provides a short survey of the central roles of ascorbate and glutathione-the latter alone or in conjunction with S-nitrosoglutathione reductase-in controlling NO bioavailability. Other major topics include the regulation of antioxidant e...
Article
Full-text available
The fascicular (bundle) phloem (FP) distributes assimilates from photosynthetic active source leaves to sinks such as young leaves, meristems and roots. It is also involved in long-distance signaling and defence responses (Van Bel and Gaupels, 2004; Walz et al., 2004; Lough and Lucas, 2006). In the past, cucurbits were frequently used as model plan...
Article
Full-text available
In cucurbits phloem latex exudes from cut sieve tubes of the extrafascicular phloem (EFP) serving in defense against herbivores. We analyzed inducible defense mechanisms in the EFP of Cucurbita maxima after leaf damage. As an early systemic response, wounding elicited transient accumulation of jasmonates and a decrease in exudation probably due to...
Article
Full-text available
Systemic signals are perceived in distant plant tissues and initiate systemic stress resistance through priming or induction of defense responses. This chapter provides an overview of how distant tissues and organs are alerted to possible attacks by signals that move through vascular bundles. It discusses systemic wound response (SWR), systemic acq...
Article
Full-text available
The field of proteomics suffers from the immense complexity of even small proteomes and the enormous dynamic range of protein concentrations within a given sample. Most protein samples contain a few major proteins, which hamper in-depth proteomic analysis. In the human field, combinatorial hexapeptide ligand libraries (CPLL; such as ProteoMiner) ha...
Article
Full-text available
Nitric oxide (NO) is now recognised as a crucial player in plant defence against pathogens. Considerable progress has been made in defining upstream and downstream signals of NO. Recently, MAP kinases, cyclic nucleotide phosphates, calcium and phosphatidic acid were demonstrated to be involved in pathogen-induced NO-production. However, the search...
Article
Full-text available
Nitric oxide (NO) is gaining increasing attention as a regulator of diverse (patho-)physiological processes in plants. Although this molecule has been described as playing a role in numerous conditions, its production, turnover and mode of action are poorly understood. Recent studies on NO production have tended to highlight the questions that stil...
Article
Full-text available
Sieve tubes are transport conduits not only for photoassimilates but also for macromolecules and other compounds that are involved in sieve tube maintenance and systemic signalling. In order to gain sufficient amounts of pure phloem exudates from barley plants for analyses of the protein and mRNA composition, a previously described stylectomy set-u...
Article
Full-text available
Vascular tissue was recently shown to be capable of producing nitric oxide (NO), but the production sites and sources were not precisely determined. Here, NO synthesis was analysed in the phloem of Vicia faba in response to stress- and pathogen defence-related compounds. The chemical stimuli were added to shallow paradermal cortical cuts in the mai...
Article
This study investigated advantages and drawbacks of two sieve-tube sap sampling methods for comparison of phloem proteins in powdery mildew-infested vs. non-infested Hordeum vulgare plants. In one approach, sieve tube sap was collected by stylectomy. Aphid stylets were cut and immediately covered with silicon oil to prevent any contamination or mod...
Article
Full-text available
In transport phloem, photoassimilates escaping from the sieve tubes are released into the apoplasmic space between sieve element (SE)/companion cell (CC) complexes (SE/CCs) and phloem parenchyma cells (PPCs). For uptake respective retrieval, PPCs and SE/CCs make use of plasma membrane translocators energized by the proton motive force (PMF). Their...
Article
Full-text available
SUMMARY Despite a long-standing notion of long-distance signals triggering systemic acquired resistance (SAR), the translocation pathway and the identity of the signals involved have not been determined with any degree of certainty. A critical assessment indicates that, in parallel to signalling via the phloem, alternative modes for SAR induction s...
Chapter
Long-distance communication between organs by physical and chemical signals along the phloem is an established phenomenon. For instance, action potentials effecting on distant ion channels in Mimosa pudica are well-known long-distance messengers (Lüttge et al. 2002). Propagation of electric signals may be mainly associated with the phloem pathway (...
Article
Full-text available
In dieser Arbeit wurden physiologische und molekulare Muster von Reaktionen des Phloems auf diverse Reize untersucht. Im ersten Teil der Arbeit wurden mit Hilfe des cDNA-AFLP (cDNA-amplified fragment length polymorphism) Teilsequenzen von 11 Transkripten im Phloemsaft aus Gerstenpflanzen ermittelt. Drei Transkripte, zu denen auch die mRNA des Abweh...

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Projects

Projects (3)
Project
Deciphering defence signalling within and via the phloem. Deciphering defence responses of the phloem. Phloem exudates are sampled e.g. by aphid stylectomy and EDTA facilitated exudation from various plant species including pumpkin, barley, and Arabidopsis. The stress-induced regulation of proteins, mRNAs, and phytohormones is analyzed. We also investigate redox-modifications of phloem proteins such as oxidation, S-nitrosylation, and tyrosine nitration.
Project
NO2 triggered basal pathogen resistance or hypersensitive cell death in a dose-dependent manner. Therefore, we used NO2 fumigations to screen 14.000 Arabidopsis T-DNA insertion mutants and 220 natural accessions for altered NO2-induced leaf damage assuming that this would lead to the identification of genes involved in pathogen resistance and hypersensitive cell death. We identified >70 candidate genes, and corresponding mutants are currently tested in pathogen infection experiments.
Project
My current research projects are focussed on the role of nitric oxide, nitrogen dioxide and cyclic nucleotides both in local as well as systemic plant defence responses.