Françoise Corbineau

Françoise Corbineau
Sorbonne Université | UPMC · Biologie du développement - UMR 7622

Professor

About

184
Publications
58,261
Reads
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9,696
Citations
Additional affiliations
January 2014 - December 2018
Sorbonne Université
Position
  • Senior Researcher
January 2009 - present
University of Freiburg
January 2008 - December 2009
Polytech Paris-UPMC
Education
September 1979 - June 1983
Sorbonne Université
Field of study
  • Plant Physiology

Publications

Publications (184)
Article
In barley, incubation of primary dormant (D1) grains on water under conditions that do not allow germination, i.e. 30°C in air and 15°C or 30°C in 5% O2, induces a secondary dormancy (D2) expressed as a loss of the ability to germinate at 15°C in air. The aim of this study was to compare the proteome of barley embryos isolated from D1 grains and D2...
Article
Primary dormant seeds of Arabidopsis thaliana did not germinate in darkness at temperature higher than 10°C-15°C. Ethylene improved the germination of dormant wild type (Col-0) seeds at 25°C in darkness but seeds of the mutant affected in the proteolytic N-degron pathway, proteolysis6 (prt6), were insensitive to ethylene suggesting that PRT6 was in...
Article
At harvest sunflower seeds (Helianthus annuus L.) are dormant and poorly germinate at 10 and 25 °C. Seed sensitivity to low temperatures (10 °C) results from an embryo dormancy whereas the envelopes (pericarp and seed coats) inhibit the germination at 25 °C. Removal of pericarp greatly improved seed germination when the germination of whole achenes...
Article
Full-text available
Temperature is the primary factor that affects seed dormancy and germination. However, the molecular mechanism that underlies its effect on dormancy alleviation remained largely unknown. In this study, we investigate hormone involvement in temperature induced germination as compared to that caused by after-ripening. Dormant (D) sunflower seeds cann...
Article
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Ethanol fermentation is considered as one of the main metabolic adaptations to ensure energy production in higher plants under anaerobic conditions. Following this pathway, pyruvate is decarboxylated and reduced to ethanol with the concomitant oxidation of NADH to NAD +. Despite its acknowledgement as an essential metabolic strategy, the conservati...
Article
Full-text available
Dormant Arabidopsis (Arabidopsis thaliana) seeds do not germinate easily at temperatures higher than 10–15 °C. Using mutants affected in ethylene signaling (etr1, ein2 and ein4) and in the N-end-rule pathway of the proteolysis (prt6 and ate1-ate2) we have investigated the effects of cold and ethylene on dormancy alleviation. Ethylene (10–100 ppm) a...
Article
Temperature is an important environmental factor affecting seed dormancy and germination. The mechanism by which temperature induces germination in dormant seeds is however still unclear. Proteomic study has been performed in dormant sunflower seeds during imbibition at permissive and non-permissive temperatures for germination, 20 and 10 °C, respe...
Article
Full-text available
Dormancy is an adaptive trait that blocks seed germination until the environmental conditions become favorable for subsequent vegetative plant growth. Seed dormancy is defined as the inability to germinate in favorable conditions. Dormancy is alleviated during after-ripening, a dry storage period, during which dormant (D) seeds unable to germinate...
Article
Temperature is an important environmental factor affecting seed dormancy and germination. The mechanism by which temperature induces germination in dormant seeds is however still unclear. Proteomic study has been performed in dormant sunflower seeds during imbibition at permissive and non-permissive temperatures for germination, 20 and 10 °C, respe...
Article
Full-text available
The mother plant plays an important dynamic role in the control of dormancy of her progeny seed, in response to environmental signals. In order to further understand the mechanisms by which this dormancy control takes place we conducted a forward genetic screen to isolate mutants which fail to enter dormancy in response to variation in temperature...
Article
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As in other cultivated species, dormancy can be seen as a problem in cereal production, either due to its short duration or to its long persistence. Indeed, cereal crops lacking enough dormancy at harvest can be exposed to pre-harvest sprouting damage, while a long-lasting dormancy can interfere with processes that rely on rapid germination, such a...
Article
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Increased tolerance of crops to low oxygen (hypoxia) during flooding is a key target for food security. In Arabidopsis thaliana (L.) Heynh., the N-end rule pathway of targeted proteolysis controls plant responses to hypoxia by regulating the stability of group VII ethylene response factor (ERFVII) transcription factors, controlled by the oxidation...
Article
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Cryptochromes are widespread blue-light absorbing flavoproteins with important signaling roles. In plants they mediate de-etiolation, developmental and stress responses resulting from interaction with downstream signaling partners such as transcription factors and components of the proteasome. Recently, it has been shown that Arabidopsis cry1 activ...
Article
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Ethylene is an important component of the gaseous environment, and regulates numerous plant developmental processes including seed germination and seedling establishment. Dormancy, the inability to germinate in apparently favorable conditions, has been demonstrated to be regulated by the hormonal balance between abscisic acid (ABA) and gibberellins...
Presentation
Full-text available
Significant progress has been made in the development of maritime pine somatic embryogenesis but there are still technical issues precluding full integration of this powerful vegetative propagation system into the French breeding programme. Maritime pine somatic embryos (SEs) require a reduction in water availability (high gellan gum concentration...
Presentation
Full-text available
In maritime pine, improved protocols are now available for the whole somatic embryogenesis process i.e. from initiation of embryogenic tissue to somatic plant regeneration. However, field trials established in France from somatic plant material have consistently revealed that somatic embryos (SEs) developed at a lower initial growth rate compared t...
Article
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Cotyledonary somatic embryos (SEs) of maritime pine are routinely matured for 12 weeks before being germinated and converted to plantlets. Although regeneration success is highly dependent on SEs quality, the date of harvesting is currently determined mainly on the basis of morphological features. This empirical method does not provide any accurate...
Article
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Maritime pine somatic embryos require a reduction in water availability (high gellan gum concentration in the maturation medium) to reach the cotyledonary stage. This key switch, reported specifically for pine species, is not yet well understood. To facilitate the use of somatic embryogenesis for mass propagation of conifers, we need a better under...
Article
Germination of primary dormant barley grains is promoted by darkness and temperatures below 20°C, but is strongly inhibited by blue light. Exposure under blue light at 10°C for periods longer than 5 days, results in a progressive inability to germinate in the dark, considered as secondary dormancy. We demonstrate that the inhibitory effect of blue...
Article
Full-text available
Hyoscyamine is the dominant alkaloid of Datura stramonium, a natural plant which grows in Algeria. Scopolamine and hyoscyamine contents depend closely on the development stages of the plant. Scopolamine is at its maximum content in the young plant whereas hyoscyamine is richer in the adult plant. The amount of hyoscyamine + scopolamine increases fr...
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Chickpea production did not progress, in spite, of intensification of agricultural practices. Drought and thermal stresses were the major factors that decreased yield when the crop is generally sown in spring. Nevertheless, winter sowing has opened new opportunities for increasing chickpea production in arid areas. This culture is based on two esse...
Article
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Dormancy is an adaptive trait that enables seed germination to coincide with favorable environmental conditions. It has been clearly demonstrated that dormancy is induced by abscisic acid (ABA) during seed development on the mother plant. After seed dispersal, germination is preceded by a decline in ABA in imbibed seeds, which results from ABA cata...
Article
Full-text available
In barley, primary dormant grains did not germinate at 30 °C in air and at 15 °C in an atmosphere containing less than 10% O2, while they germinated easily at 15 °C in air. O2 tension in embryos measured with microsensors was 15.8% at 15 °C but only 0.3% at 30 °C. Incubation of grains at 30 °C is known to induce secondary dormancy in barley, and it...
Data
Full-text available
Sunflower (Helianthus annuus L.) seeds progressively lost their ability to germinate at 25°C, the optimal temperature for germination, after accelerated aging was carried out at 45°C (a temperature too high to permit germination) in water or at 76 or 100% relative humidity (RH). The deleterious effects of the high‐temperature treatment increased wi...
Article
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Primary dormant barley (Hordeum vulgare) grains germinate at 10-15°C but not at 30°C, and there exist a positive correlation between embryo ABA content after 24 h on water and the depth of dormancy. Incubation at 30°C results in a progressive loss of the ability to germinate at 15°C. This induction of a secondary dormancy is optimal after 3 days an...
Article
Full-text available
The aim of the present work was to investigate the occurrence of the cell cycle during germination as related to thermodormancy in barley (Hordeum vulgare L., cv. Pewter) grains in relation with abscisic acid (ABA) by: (i) flow cytometry to determine the progression of the cell cycle; and (ii) reverse transcription-PCR to characterize the expressio...
Article
Maximal water uptake and diffusivity were determined from experimental soaking curves using analytical solution of the second Fick’s law in four barley samples displaying distinct hydrolytic activities during malting. Water diffusivities in the whole grains, were found to vary between 5.28 and 7.61 × 10−12 m2/s, whereas those of the endosperms were...
Article
Methods of evaluation of seed quality providing accurate prediction of seed performance under field conditions are needed by the seed industry. Various physiological tests, i.e. germination tests in suboptimal conditions of temperature, oxygenation and water potential of the medium, or accelerated ageing and controlled deterioration allow sensitive...
Article
Full-text available
Plants and animals are obligate aerobes, requiring oxygen for mitochondrial respiration and energy production. In plants, an unanticipated decline in oxygen availability (hypoxia), as caused by roots becoming waterlogged or foliage submergence, triggers changes in gene transcription and messenger RNA translation that promote anaerobic metabolism an...
Article
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Freshly harvested seeds of Arabidopsis thaliana, Columbia (Col) accession were dormant when imbibed at 25°C in the dark. Their dormancy was alleviated by continuous light during imbibition or by 5 weeks of storage at 20°C (after-ripening). We investigated the possible role of reactive oxygen species (ROS) in the regulation of Col seed dormancy. Aft...
Article
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The aim of the present study was to determine whether the loss of seed germination capacity and vigour in seeds of four wild Brassicaceae species (Brassica repanda, Moricandia arvensis, Rorippa nasturtium-aquaticum and Sinapis alba) during ageing at 45°C and 90% relative humidity was related to changes in lipid peroxidation and membrane integrity....
Article
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Sunflower (Helianthus annuus L.) seed viability is affected by moisture content (MC) during ageing and is related to accumulation of hydrogen peroxide and changes in energy metabolism. The aim of the present work was to investigate the effect of ageing on DNA alteration events by RAPD (random amplification of polymorphic DNA) analysis and to determ...
Article
One of the objectives of the malting industry is to reduce the energy cost during kilning without major effect on malt quality. In this study, the impact of a low hydration steeping process on lipid transfer protein (LTP1) modifications and β-glucan breakdown was evaluated in low (LH) and high (HH) hydrated malts. LTP1 modifications analyzed by MS/...
Article
Full-text available
Seed dormancy, defined as the inability to germinate under favourable conditions, is controlled by abscisic acid (ABA) and gibberellins (GAs). Phytohormone signalling interacts with reactive oxygen species (ROS) signalling regarding diverse aspects of plant physiology and is assumed to be important in dormancy alleviation. Using dormant barley grai...
Article
Full-text available
Dormant barley grains cannot germinate at 308C and this inability to germinate is imposed mostly by the glumellae which have been suggested to limit oxygen supply to the embryo. Hypoxia imposed either artificially or by the glumellae to embryos from dormant grains, increases embryo sensitivity to abscisic acid (ABA) and promotes the accumulation of...
Article
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The micropylar endosperm cap covering the radicle in the mature seeds of most angiosperms acts as a constraint that regulates seed germination. Here, we report on a comparative seed biology study with the close Brassicaceae relatives Lepidium sativum and Arabidopsis thaliana showing that ethylene biosynthesis and signaling regulate seed germination...
Article
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Characterization of seed dormancy in woody ornamental species is a major problem since it is a relative phenomenon. At harvest, seeds are generally regarded as dormant when they germinate slowly, produce abnormal seedlings, and are unable to germinate in apparently favourable condition. In particular, they are unable to germinate within a wide rang...
Article
Full-text available
The physiological dormancy of sunflower (Helianthus annuus) embryos can be overcome during dry storage (after-ripening) or by applying exogenous ethylene or hydrogen cyanide (HCN) during imbibition. The aim of this work was to provide a comprehensive model, based on oxidative signaling by reactive oxygen species (ROS), for explaining the cellular m...
Article
Full-text available
Incubation of barley primary dormant grains at 30°C, a temperature at which they cannot germinate results in a reinforcement of their sensitivity to temperature, and in particular in a loss of their ability to germinate at 15–20°C.1 Incubation of the grains at 30°C in the presence of GA3 (1 mM) or of isolated embryos prevents this induction of seco...
Article
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Reactive Oxygen Species (ROS) are continuously produced during seed development, from embryogenesis to germination, but also during seed storage. ROS play a dual role in seed physiology behaving, on the one hand, as actors of cellular signaling pathways and, on the other hand, as toxic products that accumulate under stress conditions. ROS, provided...
Article
Full-text available
At harvest, barley seeds are dormant because their germination is difficult above 20°C. Incubation of primary dormant seeds at 30°C, a temperature at which they do not germinate, results in a loss of their ability to germinate at 20°C. This phenomenon which corresponds to an induction of a secondary dormancy is already observed after a pre-treatmen...
Article
Full-text available
Oxalic acid is thought to be a key factor of the early pathogenicity stage in a wide range of necrotrophic fungi. Studies were conducted to determine whether oxalate could induce programmed cell death (PCD) in Arabidopsis thaliana suspension cells and to detail the transduction of the signalling pathway induced by oxalate. Arabidopsis thaliana cell...
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Full-text available
A better knowledge of genetic variability of traits related to nitrogen use efficiency (NUE) is a potential strategy to optimize N fertilization and to reduce environmental pollution without decreasing marketable yield and quality. To this aim, in this study, 13 cultivars of witloof chicory were compared with three reference cultivars known for the...