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François Treussart

François Treussart
Ecole normale supérieure de Paris-Saclay · Laboratoire LuMIn

PhD

About

151
Publications
25,263
Reads
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6,212
Citations
Citations since 2016
35 Research Items
3017 Citations
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20162017201820192020202120220100200300400
Introduction
François Treussart currently works at the Department of Physics, Ecole normale supérieure de Paris-Saclay (France). François does research in Biophtononics. His most recent publication is 'Fluorescent Nanodiamond Applications for Cellular Process Sensing and Cell Tracking'.
Additional affiliations
October 2006 - present
Ecole Normale Supérieure Paris-Saclay, France
Position
  • Professor (Full)

Publications

Publications (151)
Article
Cargo transport by molecular motors along microtubules is essential for the function of eukaryotic cells, in particular neurons in which axonal transport defects constitute the early pathological features of neurodegenerative diseases. Mainly studied in motor and sensory neurons, axonal transport is still difficult to characterize in neurons of the...
Preprint
Three-dimension real-time tracking of single emitters is an emerging tool for assessment of biological behavior as intraneuronal transport, for which spatiotemporal resolution is crucial to understand the microscopic interactions between molecular motors. We report the use of second harmonic signal from nonlinear nanoparticles to localize them in a...
Preprint
Cargo transport by molecular motors along microtubules is essential for the function of eucaryotic cells, in particular neurons in which axonal transport defects constitute the early pathological features of neurodegenerative diseases. Mainly studied in motor and sensory neurons, axonal transport is still difficult to characterize in neurons of the...
Article
Endosomal transport and positioning cooperate in the establishment of neuronal compartment architecture, dynamics, and function, contributing to neuronal intracellular logistics. Furthermore, dysfunction of endo-lysosomal has been identified as a common mechanism in neurodegenerative diseases. Here, we analyzed endo-lysosomal transport when α-synuc...
Article
A 100 nm synthetic diamond particle with a large (> 4 ppm) amount of nitrogen vacancy (NV) centers has been studied. The latter exhibit lines associated with forbidden Delta m_s = 2 and allowed Delta m_s = 1 transitions in the electron paramagnetic resonance (EPR) spectra of the ground state of the NV(-) center. The luminescence intensity of partic...
Article
A 100 nm synthetic diamond particle with a large (> 4 ppm) amount of nitrogen vacancy (NV) centers has been studied. The latter exhibit lines associated with forbidden Delta m_s = 2 and allowed Delta m_s = 1 transitions in the electron paramagnetic resonance (EPR) spectra of the ground state of the NV ⁽⁻⁾ center. The luminescence intensity of parti...
Article
A 100 nm synthetic diamond particle with a large (> 4 ppm) amount of nitrogen vacancy (NV) centers has been studied. The latter exhibit lines associated with forbidden Delta m_s = 2 and allowed Delta m_s = 1 transitions in the electron paramagnetic resonance (EPR) spectra of the ground state of the NV ⁽⁻⁾ center. The luminescence intensity of parti...
Preprint
Full-text available
Endosomal transport and positioning cooperate in the establishment of neuronal compartment architecture, dynamics and function, contributing to neuronal intracellular logistics. Furthermore, endo-lysosomal dysfunction has been identified as a common mechanism in neurodegenerative diseases. Here, we analyzed endo-lysosomal transport when α-synuclein...
Article
Diamond nanoparticles (nanodiamonds) can transport active drugs in cultured cells as well as in vivo. However, in the latter case, methods allowing the determination of their bioavailability accurately are still lacking. A nanodiamond can be made fluorescent with a perfectly stable emission and a lifetime ten times longer than that of tissue autofl...
Preprint
Diamond nanoparticles (nanodiamonds) can transport active drugs in cultured cells as well as in vivo. However, in the latter case, methods allowing to determine their bioavailability accurately are still lacking. Nanodiamond can be made fluorescent with a perfectly stable emission and a lifetime ten times longer than the one of tissue autofluoresce...
Preprint
We present a new realization of the textbook experiment consisting in single-photon interference based on the pulsed, optically excited photoluminescence of a single colour centre in a diamond nanocrystal. Interferences are created by wavefront-splitting with a Fresnel's biprism and observed by registering the "single-photon clicks" with an intensi...
Article
Full-text available
We demonstrate a high-pressure, high-temperature sintering technique to form nitrogen-vacancy-nitrogen centres in nanodiamonds. Polycrystalline diamond nanoparticle precursors, with mean size of 25 nm, are produced by the shock wave from an explosion. These nanoparticles are sintered in the presence of ethanol, at a pressure of 7 GPa and temperatur...
Article
Full-text available
Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known about tissue distribution, elimination pathways and ef...
Preprint
Nanodiamonds of detonation origin are promising delivery agents of anti-cancer therapeutic compounds in a whole organism like mouse, owing to their versatile surface chemistry and ultra-small 5 nm average primary size compatible with natural elimination routes. However, to date, little is known about tissue distribution, elimination pathways and ef...
Article
Full-text available
The content of nitrogen-vacancy (NV −) colour centres in the nanodiamonds (DNDs) produced during the detonation of nitrogen-containing explosives was found to be 1.1 ± 0.3 ppm. This value is impressive for nanodiamonds of size < 10 nm with intentionally created NV − centres. The concentration was estimated from the electron paramagnetic resonance a...
Preprint
Full-text available
The local density of optical states governs an emitters lifetime and quantum yield through the Purcell effect. It can be modified by a surface plasmon electromagnetic field, but such a field has a spatial extension limited to a few hundreds of nanometers, which complicates the use of optical methods to spatially probe the emitter-plasmon coupling....
Article
We show that nitrogen is the main impurity contained in detonation nanodiamonds at a concentration of 16,000 ppm. The content of nitrogen-vacancy NV− centers in these nanodiamonds is about 2.7 ppm, which is the largest of all known types of nanodiamonds of size less than 10 nm with artificially created NV− centers. The removal of graphite-like frag...
Chapter
This chapter highlights some recently published outstanding researches that have pushed further the boundary of photoluminescent carbon nanomaterials applications to biological systems and have brought invaluable insights into cellular and organism dynamics. Several studies have shown that fluorescent nanodiamond (FND) can provide quantitative data...
Article
Full-text available
Diamond nanocrystals smaller than 100 nm (nanodiamonds) are now recognized to be highly biocompatible. They can be made fluorescent with perfect photostability by creating nitrogen-vacancy (NV) color centers in the diamond lattice. The resulting fluorescent nanodiamonds (FND) have been used since the late 2000s as fluorescent probes for short- or l...
Article
Three types of diamond nanoparticles with sizes from 5 to 1000 nm have been investigated, whose crystal lattices include nitrogen-vacancy (NV) centers: detonation nanodiamonds (DNDs), dynamic-synthesis polycrystalline diamonds, and static-synthesis single-crystal diamonds. The electron paramagnetic resonance spectra have been investigated, along wi...
Chapter
Owing to its large bandgap of 5.5 eV, diamond can host a large variety of optically active defects (color centers). The most developed ones are two defects comprising in their structure an impurity atom (nitrogen or silicon), a vacant lattice site, and a trapped electron. These color centers are denoted as NV⁻ and SiV⁻. Modern views about the struc...
Article
The local density of optical states governs an emitters’ lifetime and quantum yield through the Purcell effect. It can be modified by a surface plasmon electromagnetic field, but such a field has a spatial extension limited to a few hundreds of nanometers, complicating the use of optical methods to spatially probe emitter–plasmon coupling. Here we...
Article
Optical bio-markers have been used extensively for intracellular imaging with high spatial and temporal resolution. Extending the modality of these probes is a key driver in cell biology. In recent years, the NV centre in diamond has emerged as a promising candidate for bio-imaging with low cytotoxicity and stable photoluminescence. Here we study t...
Article
Full-text available
Optical Spectroscopy at High Spatial Resolution with Fast Electrons - Volume 23 Issue S1 - Luiz H. G. Tizei, Sophie Meuret, Noemie Bonnet, Francois Treussart, Bruno Daudin, Bruno Gayral, Romain Brourellier, Anna Tararan, Alberto Zobelli, Yung-Chang Lin, Kazu Suenaga, Luiz. F. Zagonel, Odile Stephan, Mathieu Kociak
Chapter
Charge carrier lifetime is a key parameter for understanding the physics of electronic or optical excitations. For example the excited state can unveil details of environmental influence, specifically the role of non-radiative transitions. From a practical point of view, lifetimes can largely determine the performances of devices, such as Light Emi...
Article
Brain diseases such as autism and Alzheimer's disease (each inflicting >1% of the world population) involve a large network of genes displaying subtle changes in their expression. Abnormalities in intraneuronal transport have been linked to genetic risk factors found in patients, suggesting the relevance of measuring this key biological process. Ho...
Article
Full-text available
We present evidence of near-infrared photoluminescence (PL) signature of nitrogen vacancy centers (NCVSi)− in silicon carbide (SiC). This center exhibits an S=1 ground state spin similar to the NV− center in diamond. We have performed photoluminescence excitation measurements at cryogenic temperature and demonstrated efficient photoexcitation of di...
Article
Light and Transmission Electron Microscopies (LM and TEM) hold potential in bioimaging owing to the advantages of fast imaging of multiple cells with LM and ultrastructure resolution offered by TEM. Integrated or correlated LM and TEM are the current approaches to combine the advantages of both techniques. Here we propose an alternative in which th...
Article
Ever since the discovery of fullerenes in 1985, nanocarbon has demonstrated a wide range of applications in various areas of science and engineering. Compared with metal, oxide, and semiconductor nanoparticles, the carbon-based nanomaterials have distinct advantages in both biotechnological and biomedical applications due to their inherent biocompa...
Article
Size-dependent structural and luminescent properties of the diamond polycrystals produced by shock wave synthesis followed by grinding and separation into fractions of different polycrystal median sizes (25-1000 nm) are studied by comparative Raman and luminescence spectroscopy. The intense 738 nm narrow band luminescence of the SiV-centers are obs...
Article
Full-text available
Distinctive optical properties of inorganic quantum dot (QD) nanoparticles promise highly-valuable probes for fluorescence-based detection methods, particularly for in-vivo diagnostics, cell phenotyping via multiple markers or single molecule tracking. However, despite high hopes, this promise has not been fully realized yet, mainly due to difficul...
Article
Massive parallelization of STED-like nanoscopies is now achievable using well-designed optical lattices for state depletion. Yet, only the lattice intensity distribution was considered for the description of the super-resolved point spread function. This holds for fast-rotating fluorescent emitters. Here, we study the effects of electric field topo...
Article
Full-text available
div class="title">Quantum and Time-Resolved Nano-Optics using Auto-Correlated Cathodoluminescence in a STEM - Volume 21 Issue S3 - Sophie Meuret, Luiz Tizei, Romain Bourrellier, Thomas Auzelle, François Treussart, Bruno Daudin, Alberto Zobelli, Bruno Gayral, Mathieu Kociak
Article
We have measured the second order correlation function [g(2)(τ)] of the cathodoluminescence intensity resulting from the excitation by fast electrons of defect centers in wide band-gap semiconductor nanocrystals of diamond and hexagonal boron nitride. We show that the cathodoluminescence second order correlation function g(2)(τ) of multiple defect...
Article
Full-text available
Magnetic resonance techniques (electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR)) are used for tracking the multi-stage process of the fabrication of fluorescent nanodiamonds (NDs) produced by high-energy electron irradiation, annealing, and subsequent nano-milling. Pristine commercial high pressure and high temperature mic...
Article
Full-text available
Fluorescence imaging of cells and subcellular compartments is an essential tool to investigate biological processes and to evaluate the development and progression of diseases. In particular, protein-protein interactions can be monitored by Förster resonance energy transfer (FRET) between two proximal fluorophores that are attached to specific reco...
Article
Full-text available
The cover image is a pop art representation of a shell architecture on fluorescent nanodiamond. As P. Cigler and co-workers report on page 1106, the diamond nanoparticle is embedded in a silica shell, which is terminated by a hydrophillic, azide- or alkyne-substituted PEG polymer. In contrast to unmodified nanodiamonds, this nanoarchitecture brings...
Article
Full-text available
Multiple frequency electron paramagnetic resonance (EPR) study of small (4-25 nm) nanodiamonds obtained by various dynamic synthesis techniques reveals systematic presence in the half-field (HF) region a distinctive doublet fingerprint consisting of resolved gHF1 = 4.26 and gHF2 = 4.00 signals. This feature is attributed to "forbidden" ΔMS = 2 tran...
Conference Paper
In this presentation we show that the autocorrelation function of the cathodo- luminescence signal (CL−g(2)(τ)) can be different from the photoluminescence PL−g(2)(τ) showing a huge nanosecond bunching effect g(2)(0) > 1, allowing to retrieve emitters lifetime at nanometer scale.
Article
Nitrogen-vacancy (NV) colour centres in nanodiamonds are highly promising for bio-imaging and sensing. However, resolving individual NV centres within nanodiamond particles and the controlled addressing and read-out of their spin state has remained a major challenge. Spatially stochastic super-resolution techniques cannot provide this capability on...
Article
Measurements of the photon second-order correlation function, g(2)(τ), is a common tool for the characterization of single photon emitters, like nitrogen-vacancy color centers in diamond. Such measurement requires background photoluminescence correction, which is easy when this background is homogeneous on a few wavelengths scale. However, if the s...
Article
Full-text available
We propose using an optical cavity to enhance the sensitivity of a magnetometer relying on the detection of the spin state of a high-density nitrogen-vacancy ensemble in diamond using infrared optical absorption. The role of the cavity is to obtain a contrast in the absorption-detected magnetic resonance approaching unity at room temperature. We pr...
Article
Full-text available
Diamond nanocrystals containing Nitrogen-Vacancy (NV) color centers have been used in recent years as fluorescent probes for near-field and cellular imaging. In this work we report that an infrared (IR) pulsed excitation beam can quench the photoluminescence of NV color center in a diamond nanocrystal (size < 50 nm) with an extinction ratio as high...
Article
Full-text available
Small interfering RNAs (siRNAs) are powerful tools commonly used for the specific inhibition of gene expression. However, vectorization is required to facilitate cell penetration and to prevent siRNA degradation by nucleases. We have shown that diamond nanocrystals coated with cationic polymer can be used to carry siRNAs into Ewing sarcoma cells, i...
Data
Flow cytometer measurement of apoptotic cell population (Anexin V+/Propidiume Iodide−) after treatment of NIH/3T3 EWS-Fli1 cells by various compounds. Only the treatment by the AntiSens (AS) siRNA directed against EWS-Fli1 oncogene and vectorized by ND-PEI leads to an increase in apoptotic cell number. siRNA Ct is the 18S siRNA sequence. (EPS)
Data
RT-qPCR measurement of EWS-Fli1 mRNA targeted by siRNA with AntiSens (AS) action in NIH/3T3 EWS-Fli1 cells. siRNA is vectorized by either cationic ND (ND-PAH or ND-PEI) or free polycations (PAH or PEI). siRNA:ND-PEI promotes 40% inhibition of mRNA expression while free PEI and free PAH have no inhibiting effect. (EPS)
Data
TEM observations of ND-PAH of size ≲≲200 nm in NIH/3T3 EWS-Fli1 cells after 4 h of incubation. Almost no ND-PAH are uptaken by the cells (A). A few nanocrystals are observed at the surface (B) and some are detected in lysosomal and endosomal compartments (C). Scale bars in (A): 2 µm and in (B) and (C): 500 nm. TEM magnification in (A): ×4,400, and...
Data
Full-text available
This file contains three sections: section 1 on the “Incidence of EWS-Fli1 inhibition by siRNA:polycationic ND on apoptotic status of NIH/3T3 EWS-Fli1 cells”, section 2 on the “Comparison of siRNA inhibition of EWS-Fli1 expression in NIH/3T3 EWS-Fli1 cells when it is vectorized by free PEI (PAH), or ND-PEI (resp. ND-PAH)”, and finally section 3 on...
Conference Paper
Full-text available
Background / Purpose: The eukaryotic microorganism, Dictyostelium discoideum, participates in the evolutionary production of extracellular vesicles. These vesicles, evidenced as a multidrug cell detoxification mechanism, are constitutively secreted during cell growth and starvation-induced aggregation. These vesicles, involved in Dictyostelium in...
Data
Title: Microvesicles of Dictyostelium as a Model of Eukaryotic Extracellular Vesicles
Article
Neurons display dendritic spines plasticity and morphology anomalies in numerous psychiatric and neurodegenerative diseases. These changes are associated to abnormal dendritic traffic that can be evidenced by fluorescence microscopy. As a fluorescent probe we propose to use fluorescent diamond nanoparticles with size of < 50 nm. Color centers embed...
Article
Full-text available
Fluorescent nanodiamonds (FNDs) have recently developed into an exciting new tool for bioimaging applications. The material possesses several unique features including high biocompatibility, easy bioconjugation, and perfect photostability, making it a promising optical nanoprobe in vitro as well as in vivo. This work explores the potential applicat...
Article
The ability of diamond nanoparticles (nanodiamonds, NDs) to deliver small interfering RNA (siRNA) into Ewing sarcoma cells is investigated with a view to the possibility of in-vivo anticancer nucleic-acid drug delivery. siRNA is adsorbed onto NDs that are coated with cationic polymer. Cell uptake of NDs is demonstrated by taking advantage of the ND...
Article
Full-text available
We studied the parameters to optimize the production of negatively charged nitrogen-vacancy color centers (NV-) in type-1b single crystal diamond using proton irradiation followed by thermal annealing under vacuum. Several samples were treated under different irradiation and annealing conditions and characterized by slow positron beam Doppler-broad...
Conference Paper
Full-text available
Single negatively charged nitrogen-vacancy (NV<sup>-</sup>) defect centres in diamond are attractive systems for the implementation of solid state quantum information processing (QIP) and quantum key distribution (QKD). In both approaches, the coupling of an individual NV<sup>-</sup> centre to a resonant microcavity is of interest: it would improve...
Article
Full-text available
The photoluminescence of nitrogen-vacancy (NV) centers in diamond nanoparticles exhibits specific properties as compared to NV centers in bulk diamond. For instance large fluctuations of lifetime and brightness from particle to particle have been reported. It has also been observed that for nanocrystals much smaller than the mean luminescence wavel...
Article
Full-text available
We present here both theoretical and experimental results on the fluorescence of single defect centers in diamond nanocrystals embedded in a planar dielectric microcavity. From a theoretical point of view, we show that the overall fluorescence collection efficiency using a moderate numerical aperture microscope objective can be enhanced by using a...
Article
Full-text available
We present both theoretical and experimental results on fluorescence of single defect centers in diamond nanocrystals embedded in a planar dielectric microcavity. From a theoretical point of view, we show that the overall fluorescence collection efficiency using moderate numerical aperture microscope objective can be enhanced by using a low quality...
Article
Nanodiamond powders with an average size of 50nm have been irradiated using high-energy electron beam. After annealing and chemical treatment, nanodiamond colloidal solutions were obtained and deposited on silica coverslips by spin-coating. The fluorescence of nanodiamonds was studied by confocal microscopy together with atomic force microscopy. We...
Article
Full-text available
We present a study of the charge state conversion of single nitrogen-vacancy (NV) defects hosted in nanodiamonds (NDs). We first show that the proportion of negatively-charged NV$^{-}$ defects, with respect to its neutral counterpart NV$^{0}$, decreases with the size of the ND. We then propose a simple model based on a layer of electron traps locat...
Article
Nitrogen-Vacancy (NV) color centers in diamond nanoparticles (NanoDiamonds, NDs) have a great number of applications, in particular in quantum information processing and as cellular fluorescent labels. In this work we compare the photoluminescence properties of a single NV color center embedded in a 30 nm diameter nanodiamond to that of a single or...
Article
Full-text available
We recently (Optics Express 17, 19969 (2009)) introduced an all-optical method for grafting onto the apex of an optical tip a single 20 nm nanodiamond with single color-center occupancy and used the resulting single-photon tip in scanning near-field imaging at room temperature, thereby achieving a genuine scanning single-photon microscopy working i...
Article
Full-text available
The controlled and coherent manipulation of individual quantum systems is a fundamental key for the development of quantum information processing. The nitrogen-vacancy (NV) color center in diamond is a promising system since its photoluminescence is perfectly stable at room temperature and its electron spin can be optically read-out at the individu...