François Coutte

François Coutte
Université de Lille · UMRt BioEcoAgro

Associate Professor in Biotechnology

About

50
Publications
8,600
Reads
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1,003
Citations
Additional affiliations
June 2014 - present
LIPOFABRIK SAS
Position
  • Consultant
September 2010 - present
Polytech Lille
Position
  • Lecturer in Microbiology/Biotechnology
September 2010 - present
Polytech Lille
Position
  • Lecturer
Description
  • Teaching in Microbiology, Biotechnology, Molecular Biology
Education
September 2005 - September 2009
Université de Lille
Field of study
  • Biological Function Engineering
September 2004 - September 2005
Université de Lille
Field of study
  • Biotechnology
September 2003 - September 2004
Université de Lille
Field of study
  • Therapeutic Chemistry

Publications

Publications (50)
Article
Full-text available
Apple scab is an important disease conventionally controlled by chemical fungicides, which should be replaced by more environmentally friendly alternatives. One of these alternatives could be the use of lipopeptides produced by Bacillus subtilis. The objective of this work is to study the action of the three families of lipopeptides and different m...
Chapter
Today, plant production is increasing, but most industrial processes generate a lot of waste and by-products for which, in the current context, it is a priority to recycle or valorize them. One of the cheapest valorization routes is fermentation, in particular lactic fermentation by Lactobacillus species, which produces lactic acid and other molecu...
Presentation
Zymoseptoria tritici, causing Septoria tritici blotch, is one of the most agro-economically damaging pathogens on wheat crops worldwide, causing up to 50% yield losses. In the current context encouraging agroecology and the increasing pressure to reduce the use of conventional pesticides in agriculture, new eco-friendly solutions, such as biocontro...
Article
Full-text available
This study investigated the antiradical and antioxidant potential of the three families of lipopeptides (i.e., surfactin, mycosubtilin, and plipastatin/fengycin) produced by Bacillus subtilis strains. The antiradical/antioxidant activities of highly purified lipopeptides were studied in acellular models using a 2,2-diphenyl-1-picrylhydrazyl (DPPH)...
Article
Full-text available
Mycosubtilin belongs to the family of lipopeptides. Different isoforms with various anti-fungal activities can be obtained according to the length and the isomery of the fatty acid. In this work, the activities of the mycosubtilin isoforms were first studied against the pathogen Aspergillus niger, revealing the high activity of the anteiso-C17 isof...
Article
Full-text available
The exploration of certain microbial resources such as beneficial endophytic microorganisms is considered a promising strategy for the discovery of new antimicrobial compounds for the pharmaceutical industries and agriculture. Thirty-one endophytic bacterial strains affiliated with Bacillus, Janthinobacterium, Yokenella, Enterobacter, Pseudomonas,...
Article
Full-text available
Lipopeptides produced by Bacillus subtilis display many activities (surfactant, antimicrobial, and antitumoral), which make them interesting compounds with a wide range of applications. During the past years, several processes have been developed to enable their production and purification with suitable yield and purity. The already implemented pro...
Article
Full-text available
BACKGROUND Lipopeptides extracted from the Bacillus genus are emerging biopesticides, especially in protecting crops against phytopathogens. Among the three main families of lipopeptides, Surfactins have been identified as having insecticidal properties against several insect orders. However, the sublethal effects of these promising biopesticides r...
Poster
Full-text available
Lipopeptides extracted from the Bacillus genus are emerging biopesticides, especially to protect crops against phytopathogens. Among the three main families of lipopeptides, Surfactin has been identified has having insecticidal properties against several insect orders. However, the sublethal effects of this promising biopesticide on insect pests an...
Article
Full-text available
Within the framework of biocontrol development, several natural lipopeptides produced by Bacillus subtilis show well-documented anti-microbial properties, especially in orchards. However, the number of studies on their putative insecticidal effects remain low despite the growing interest to develop new strategies of orchards pests' control. The ros...
Article
Full-text available
An original culture process in bioreactor named overflowing continuous culture (O-CC) was developed to produce and recover continuously mycosubtilin, a lipopeptide antifungal biosurfactant of major interest. The lipopeptide production was first investigated in shake conical flasks in different culture media (ACS, DSM and Landy), followed by a pH co...
Chapter
Bacillus subtilis, the model strain of Gram-positive bacteria, is under continuous domestication to competitively perform at the industrial level. In the last decades the outputs of fundamental research on this bacterium have generated huge data that helped engineers in building approaches to tinker robust and efficient cells. The recorded progress...
Article
Full-text available
Lipopeptide biosurfactants produced by Bacillus sp. were assessed regarding their antimicrobial activity against foodborne pathogenic and food spoilage microorganisms. Both Gram-positive and Gram-negative bacteria were found not to be susceptible to these lipopeptides. However, mycosubtilin and mycosubtilin/surfactin mixtures were very active again...
Article
Full-text available
Production of bioactive peptides (BAPs) by Lactobacillus species is a cost-effective approach compared to the use of purified enzymes. In this study, proteolytic Lactobacillus helveticus strains were used for milk fermentation to produce BAPs capable of inhibiting angiotensin converting enzyme (ACE). Fermented milks were produced in bioreactors usi...
Preprint
Natural isolates of Bacillus subtilis are known for their ability to produce a large panel of bioactive compounds. Unfortunately, their recalcitrance to conventional molecular techniques limits their transcript studies. In this work, diYculties to isolate RNA attributed to the cell wall were overcome, Wnally authorising powerful RT-PCR's.
Article
Full-text available
Biofilm bioreactors are promising systems for continuous biosurfactant production since they provide process stability through cell immobilization and avoid foam formation. In this work, a two-compartment biofilm bioreactor was designed consisting of a stirred tank reactor and a trickle-bed reactor containing a structured metal packing for biofilm...
Article
The aim of this study was to investigate the probiotic properties of 174 Lactobacillus strains isolated from Mongolian dairy products, and particularly their impact on intestinal calcium uptake and absorption. All isolates underwent a first screening based on cell surface hydrophobicity, acid tolerance, tolerance to gastro-intestinal digestion, aut...
Article
Full-text available
Within the framework of biocontrol development, three natural substances produced by Bacillus subtilis, called lipopeptides, have been studied: fengycin (F), surfactin (S), and mycosubtilin (M). Their antifungal properties were tested in vitro, in liquid medium, on two strains of Venturia inaequalis, ascomycete fungi causing apple scab. These two s...
Article
Biofilm bioreactors have already been proven to be efficient systems for microbial lipopeptide production since they avoid foam formation. However, the cell adhesion capacities of the laboratory strain B.subtilis 168 to the biofilm bioreactor support are limited. In this work, we present a novel approach for increasing cell adhesion through the gen...
Article
The aim of this study was to evaluate the effect of spreading the lipopeptide surfactin, for short time (10/20 s), on dentin wettability. Study groups were surfactin: 2.8; 1.4; 0.7; 0.35; and 0.175 mg/mL and a control group that received no treatment. Dentin discs (4 mm height) were prepared and polished with 600-grit SiC paper. Contact angle deter...
Article
Lipopeptides, such as surfactins are important biosurfactants produced by Bacillus sp. that find applications in many areas (environment, medicine and food industries). Giving their importance, the use of simple detection methods will facilitate screening and quantification. In the present work, we described a completely automated workflow for the...
Article
The aim of this study was to investigate the probiotic properties of 174 Lactobacillus strains isolated from Mongolian dairy products, and particularly their impact on intestinal calcium uptake and absorption. All isolates underwent a first screening based on cell surface hydrophobicity, acid tolerance, tolerance to gastro-intestinal digestion, aut...
Article
Full-text available
Background Lipopeptides are a promising group of surface-active compounds of microbial origin (biosurfactants). These diverse molecules are produced mainly by Bacillus and Pseudomonas strains. Because of their attractive physiochemical and biological properties, biosurfactants are considered to be “green and versatile molecules of the future”. The...
Patent
Full-text available
Biosurfactants produced by a strain of Bacillus sp and to uses thereof . A composition comprising the biosurfactants , and a method for producing the biosurfactants . A method for obtaining a biosurfactant , and a device for implementing the method . The production of biopesticides or biosurfactants for the phytosanitary industry , and in the field...
Article
Surfactin, a lipopeptide produced by Bacillus subtilis, is one of the most powerful biosurfactants known. This molecule consists of a cyclic heptapeptide linked to a ?-hydroxy fatty acid chain. The isomery and the length of the fatty acid (FA) chain are responsible for the surfactin's activities. In this study, the gene codY, which encode for the g...
Article
Full-text available
Innovations in novel enzyme discoveries impact upon a wide range of industries for which biocatalysis and biotransformations represent a great challenge, i.e., food industry, polymers and chemical industry. Key tools and technologies, such as bioinformatics tools to guide mutant library design, molecular biology tools to create mutants library, mic...
Chapter
Use of a membrane within a bioreactor (MBR), either microbial or enzymatic, is a technology that has existed for 30. years to increase process productivity and/or facilitate the recovery and the purification of biomolecules. Currently, this technology is attracting increasing interest in speeding up the process and in better sustainability. In this...
Book
Use of a membrane within a bioreactor (MBR), either microbial or enzymatic, is a technology that has existed for 30 years to increase process productivity and/or facilitate the recovery and the purification of biomolecules. Currently, this technology is attracting increasing interest in speeding up the process and in better sustainability. In this...
Article
Biosurfactants are important in many areas; however, costs impede large-scale production. This work aimed to develop a global sustainable strategy for the production of biosurfactants by a novel strain of Bacillus amyloliquefaciens. Initially, Bacillus sp. strain 0G was renamed B. amyloliquefaciens subsp. plantarum (syn. Bacillus velezensis) after...
Article
We wish to predict changes of reaction networks with partial kinetic information that lead to target changes of their steady states. The changes may be either increases or decreases of influxes, reaction knockouts, or multiple changes of these two kinds. Our prime applications are knockout prediction tasks for metabolic and regulation networks. In...
Article
Culture medium elements were analysed by a screening DoE to identify their influence in surfactin specific production by a surfactin constitutive overproducing Bacillus subtilis strain. Statistics pointed the major enhancement caused by high glutamic acid concentrations, as well as a minor positive influence of tryptophan and glucose. Successively,...
Conference Paper
Full-text available
We propose a formal modeling language for reaction networks with partial kinetic information. The language has a graphical syntax reminiscent to Petri nets. The kinetics of reactions need to be described only partially, so that the language can be used to model the regulation of metabolic networks. We present a qualitative reasoning method based on...
Article
In this study, we demonstrate the potential of Bacillus subtilis BBG111 to trigger ISR in rice (Oryza sativa L.) against Rhizoctonia solani, while there was no effect against Magnaporthe oryzae. We show that plant recognition of BBG111 induces jasmonic acid (JA) and ethylene (ET) as well as abscisic acid (ABA) and auxin signaling. In addition, B. s...
Article
A Bacillus subtilis mutant strain overexpressing surfactin biosynthetic genes was previously constructed. In order to further increase the production of this biosurfactant, our hypothesis is that the surfactin precursors, especially leucine, must be overproduced. We present a three step approach for leucine overproduction directed by methods from c...
Conference Paper
We present a new modeling language for reaction networks with partial kinetic information, that supports metabolic flux analysis and knockout prediction for all models written in this language. In our language, reaction networks are modeled by graphs similar to Petri nets, except that reactions are annotated by suitable partial descriptions of kin...
Article
Full-text available
Non-self recognition of microorganisms partly relies on the perception of microbe-associated molecular patterns (MAMPs) and leads to the activation of an innate immune response. Bacillus subtilis produces three main families of cyclic lipopeptides (LPs), namely, surfactins, iturins and fengycins. Although LPs have been involved in ISR activation, l...
Article
The use of surfactin and mycosubtilin as an eco-friendly alternative to control lettuce downy mildew caused by the obligate pathogen Bremia lactucae was investigated. Preliminary ecotoxicity evaluations obtained from three different tests revealed the rather low toxicity of these lipopeptides separately or in combination. The EC50 (concentration es...
Article
Full-text available
The self-assembly in aqueous solution of three lipopeptides obtained from Bacillus subtilis has been investigated. The lipopeptides surfactin, plipastatin and mycosubtilin contain distinct cyclic peptide headgroups as well as differences in alkyl chain length, branching and chain length distribution. Cryogenic transmission electron microscopy and X...
Article
The aim of this work was to investigate the lipopeptides aggregation behavior in single and mixed solutions in a wide range of concentrations, in order to optimize their separation and purification following the two-step ultrafiltration process and using large pore size membranes (up to MWCO = 300 kDa). Micelle size was determined by dynamic light...
Article
Full-text available
Recently, a bubbleless membrane bioreactor (BMBR) has been successfully developed for biosurfactant production by Bacillus subtilis[1]. In this study, for the first time, continuous culture were carried out for the production of surfactin in a BMBR, both with or without a coupled microfiltration membrane. Results from continuous culture showed that...
Article
Three families of lipopeptides (LPs), surfactin, iturin (including mycosutilin) and fengycin, produced by the rhizabacterium Bacillus subtilis have received considerable attention for their antimicrobial, cytotoxic, antitumor, immunosuppressant and surfactant properties. Among them, iturins and fengycins generally display a strong in vitro antifung...
Article
In conventional agricultural practices, fungicides are extensively used to control the development of many fungal plant pathogens. However, the reduction of pesticide applications in the field, which is recommended by authorities and approved by public opinion, may lead to a re-emergence of diseases. Alternative strategies have therefore to be deve...
Article
This paper formulates a feeding law for a bioprocess dedicated to the production of an antibiotic surfactant using Bacillus subtilis. The specificity of the process relies on the use of the surface active property of the product to extract it by foaming. The control law is designed to maintain a constant specific biomass growth rate while taking in...
Article
Surfactin and fengycin are lipopeptide biosurfactants produced by Bacillus subtilis. This work describes for the first time the use of bubbleless bioreactors for the production of these lipopeptides by B. subtilis ATCC 21332 with aeration by a hollow fiber membrane air-liquid contactor to prevent foam formation. Three different configurations were...
Article
To analyse the effects of plipastatin operon disruption and constitutive expression of surfactin operon in Bacillus subtilis 168 on surfactin productivity, in vitro invasive growth and antagonism against fungi. The srfA native promoter was replaced by the constitutive promoter P(repU) in B. subtilis 168 after integration of a functional sfp gene. M...
Article
Natural isolates of Bacillus subtilis are known for their ability to produce a large panel of bioactive compounds. Unfortunately, their recalcitrance to conventional molecular techniques limits their transcript studies. In this work, difficulties to isolate RNA attributed to the cell wall were overcome, finally authorising powerful RT-PCR's.

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Projects

Projects (5)
Project
The BIOSMART project proposal will develop active and smart bio-based and compostable packages to meet the needs of both fresh and pre-treated food applications. In addition, the novel packaging system will form the basis for tailoring performance and functionality to specific flexible and rigid food packages in diverse market segments.