Franck Gallardo

Franck Gallardo
NeoVirTech SAS · infectious disease

PhD

About

44
Publications
5,759
Reads
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730
Citations
Citations since 2016
32 Research Items
565 Citations
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2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
Introduction
- Project design/management - Autofluorescent viral vector/virus model development/validation - Management of high content screening campaign - IP protection/partnering - communication with investor/media Strong experience in antiviral discovery by HCS Member of the World Society of Virology (early stage)

Publications

Publications (44)
Article
Full-text available
The ongoing monkeypox virus (MPXV) outbreak is the largest ever recorded outside of Africa. We isolated and sequenced a virus from the first clinical MPXV case diagnosed in France (May 2022). We report that tecovirimat (ST-246), a US Food and Drug Administration approved drug, is efficacious against this isolate in vitro at nanomolar concentrations...
Preprint
The ongoing monkeypox virus (MPXV) outbreak is the largest ever recorded outside of Africa. Genomic analysis revealed a divergent phylogenetic lineage within clade 3, and atypical clinical presentations have been noted. We report the sequencing and isolation of the virus from the first clinical case diagnosed in France in May 2022. We tested the in...
Article
Full-text available
Together with local chromatin structure, gene accessibility, and the presence of transcription factors, gene positioning is implicated in gene expression regulation. Although the basic mechanisms are expected to be conserved in eukaryotes, less is known about the role of gene positioning in plant cells, mainly due to the lack of a highly resolutive...
Article
Equine herpesvirus 1 (EHV-1) is a causative agent of respiratory disorders, abortion and myeloencephalopathy in horses and has an important impact on equine health and economy. Several bacterial artificial chromosomes have already been developed and enabled identification and functional characterization of EHV-1 genes. Unfortunately, little is know...
Preprint
Full-text available
RESUME Gene expression is governed by several layers of regulation which in addition to genome organization, local chromatin structure, gene accessibility and the presence of transcription factors also includes gene positioning. Although basic mechanisms are expected to be conserved in Eukaryotes, surprisingly little information on the role of gene...
Article
Full-text available
Oncolytic viruses (OV) are novel cancer gene therapies that are moving toward the forefront of modern medicines. However, their full therapeutic potential is hindered by the lack of convenient and reliable strategies to visualize and quantify OV growth kinetics and therapeutic efficacy in live cells. Here, we present a first-in-class imaging approa...
Article
Full-text available
As a live biologic agent, oncolytic vaccinia virus has the ability to target and selectively amplify at tumor sites. We have previously reported that deletion of thymidine kinase and ribonucleotide reductase genes in vaccinia virus can increase the safety and efficacy of the virus. Here, to allow direct visualization of the viral genome in living c...
Article
Twenty-nine fluorinated corroles were prepared, spectroscopically characterized, and studied for their antiviral activity against human cytomegalovirus infection. Six corroles were also fully characterized by X-ray crystallography giving insights on their geometrical features. The halogenated corroles reported herein exhibit significantly improved...
Article
Human cytomegalovirus (hCMV) is responsible for several pathologies impacting immunocompromised patients and can trigger life-threatening infection. Several antivirals are available and are used in the clinic, but hCMV resistant strains have appeared and patients have encountered therapeutic failure. Hence, there is a constant need for new best in...
Article
Full-text available
Many steps in the baculovirus life cycle, from initial ingestion to the subsequent infection of all larval cells, remain largely unknown; primarily because it has hitherto not been possible to follow individual genomes and their lineages. Use of ANCHORTM technology allows a high intensity fluorescent labelling of DNA. When applied to a virus genome...
Article
Full-text available
The guanabenz derivative Sephin1 has recently been proposed to increase the levels of translation initiation factor 2 (eIF2α) phosphorylation by inhibiting dephosphorylation by the protein phosphatase 1—GADD34 (PPP1R15A) complex. As phosphorylation of eIF2α by protein kinase R (PKR) is a prominent cellular antiviral pathway, we evaluated the conseq...
Article
Full-text available
Purpose The Epstein–Barr virus (EBV)-associated cancer nasopharyngeal carcinoma (NPC) is rare in Europe and North America but is a real public health problem in some regions of the world, such as southern Asia, North Africa, and for Inuit populations. Due to the anatomy and location of the nasopharynx, surgery is rarely used to treat primary NPC ca...
Article
Full-text available
Adenoviruses are DNA viruses with a lytic infection cycle. Following the fate of incoming as well as recently replicated genomes during infections is a challenge. In this study, we used the ANCHOR3 technology based on a bacterial partitioning system to establish a versatile in vivo imaging system for adenoviral genomes. The system allows the visual...
Article
Full-text available
Human cytomegalovirus (HCMV) induces latent lifelong infections in all human populations. Between 30% and nearly 100% of individuals are affected depending on the geographic area and socioeconomic conditions. The biology of the virus is difficult to explore due to its extreme sophistication and the lack of a pertinent animal model. Here, we present...
Patent
La présente invention a trait au domaine des systèmes rapporteurs. Plus particulièrement, la présente invention concerne un baculovirus modifié exprimant un système rapporteur permettant son identification directe par fluorescence. L'invention concerne également la quantification d'une population de baculovirus marqués, ainsi que l'utilisation de c...
Preprint
Human cytomegalovirus (HCMV) induces latent life-long infections in all human populations. Depending on geographic area and socio-economic conditions between 30 to nearly 100% of individuals are affected. The biology of this virus is difficult to explore due to its extreme sophistication and the lack of pertinent animal model. Here we present the f...
Data
Movie S2. Representative Cells Displaying an ANCHOR-Tagged CCND1 Transgene, OR3-Santaka, Red Spots, during Estradiol Activated Transcription, MCP-GFP, Green Spots, Related to Fig. 1 ANCH3CCND1-MS2 cells transiently expressing OR3-Santaka and MCP-GFP were imaged (streaming at 200 ms exposure over 200 frames) using a Nipkow-disk confocal (ANDOR) mic...
Data
Movie S1. Representative Cells, All Displaying an ANCHOR-Tagged CCND1 Transgene, Related to Fig. 1 ANCH3-CCND1-MS2 cells transiently expressing OR3-Santaka were imaged in 3D (200 ms exposure, 40 frames at 200 nm intervals in z) using a Nipkow-disk confocal (ANDOR) microscope equipped with a 60x 1.4NA objective. Pixel size was 110 nm. Images were p...
Article
Full-text available
Genome dynamics are intimately linked to the regulation of gene expression, the most fundamental mechanism in biology, yet we still do not know whether the very process of transcription drives spatial organization at specific gene loci. Here, we have optimized the ANCHOR/ParB DNA-labeling system for real-time imaging of a single-copy, estrogen-indu...
Article
Full-text available
Telomerase can generate a novel telomere at DNA double-strand breaks (DSBs), an event called de novo telomere addition. How this activity is suppressed remains unclear. Combining single-molecule imaging and deep sequencing, we show that the budding yeast telomerase RNA (TLC1 RNA) is spatially segregated to the nucleolus and excluded from sites of D...
Preprint
Genome dynamics relate to regulation of gene expression, the most fundamental process in biology. Yet we still do not know whether the very process of transcription drives spatial organization and chromatin conformation at specific gene loci. To address this issue, we have optimized the ANCHOR/ParB DNA labeling system for real-time imaging and quan...
Article
Introduction Melanoma is the most aggressive form of skin cancer. Targeted antimelanoma therapies such as BRAF V600 inhibitors have shown spectacular results. However, these therapies are restricted to BRAF V600 mutated melanoma and display both adverse effects and resistance outbreaks. Methods A high-content screening campaign identified compound...
Article
Full-text available
CD70 is a costimulatory molecule member of the Tumor Necrosis Factor family that is expressed on activated immune cells. Its ectopic expression has been described in several types of cancer cells including lymphomas, renal cell carcinomas and glioblastomas. We have recently described its expression in a part of tumor cells from the vast majority of...
Data
LB39-MEL CD70+ cells were treated with control medium (DMSO) or different doses of PLX-4032 for 72 h, and analyzed by flow cytometry for CD70 membrane expression (Figure A) or by Western Blot for CD70 global expression (Figure B). Quantification of three independent experiments is shown in Figure B-upper and a representative Western blot illustrati...
Data
CD70 silencing were checked by Western-Blot in LB1319-MEL cells (Figure A) and in WM-266-4 (Figure B) transfected with control siRNA (siNeg) or CD70 specific siRNA (siCD70). 72 h post transfection CD70 global expression was analyzed by Western-Blot (Figures A, B). (EPS)
Data
MAPK pathway activation was evaluated by Western Blot in LB1319-MEL, LB39-MEL CD70+ and WM-266-4 cells (Figure A). WM-266-4 cells were treated with 5μM of U0126 for 48 h and analyzed for CD70 global expression by Western Blot. Quantification of three independent experiments is shown in (Figure B). Results are expressed as mean values ± SD (error ba...
Data
RhoB (Figure A) et RhoC (Figure B) silencing were checked by Western Blot in LB1319-MEL cells transfected with siCtrl, siRhoB1, siRhoB2, siRhoC1 or siRhoC2 for 72 h. LB1319-MEL cells were transfected with siCtrl, siRhoA1 and siRhoA2. 72 h post transfection CD70 global expression was analyzed by Western Blot and quantified in three independent exper...
Article
Different nitroporphyrinoid derivatives were synthesized and studied as potential agents against human cytomegalovirus. Interestingly, two nitrocorroles display strong activity against human cytomegalovirus with IC50 inferior to 0.5μM. These compounds also possess antiproliferative activities without detected in vivo toxicity. Therefore, nitrocorro...
Article
Full-text available
Chromosome breakage is a major threat to genome integrity. The most accurate way to repair DNA double strand breaks (DSB) is homologous recombination (HR) with an intact copy of the broken locus. Mobility of the broken DNA has been seen to increase during the search for a donor copy. Observing chromosome dynamics during the earlier steps of HR, mai...
Article
The stable linearity of eukaryotic chromosomes depends on special characteristics of their ends, the telomeres. Accurate telomere function in turn requires a sustained presence of repeated DNA elements, which are maintained by the enzyme telomerase. The telomerase holoenzyme is composed of both protein and RNA, and its functions rely on proper expr...
Article
The telomerase, which is composed of both protein and RNA, maintains genome stability by replenishing telomeric repeats at the ends of chromosomes. Here, we use live-cell imaging to follow yeast telomerase RNA dynamics and recruitment to telomeres in single cells. Tracking of single telomerase particles revealed a diffusive behavior and transient a...
Article
Full-text available
Transcripts containing expanded CNG repeats, which are found in several neuromuscular diseases, are not exported from the nucleus and aggregate as ribonuclear inclusions by an unknown mechanism. Using the MS2-GFP system, which tethers fluorescent proteins to a specific mRNA, we followed the dynamics of single CUG-repeat transcripts and RNA aggregat...
Article
Localization of messenger RNA (mRNA) is a process used by eukaryotes to control the spatio-temporal expression of proteins involved in cellular motility, asymmetric cell division, or polarized cell growth. A better understanding of this process relies on methods to detect specifically the position of an mRNA in fixed or living cells. This chapter p...
Article
Full-text available
Rps15p, an essential ribosomal protein, was previously shown to be critical for nuclear export of small subunit pre-particles. We have designed a synthetic lethal screen in Saccharomyces cerevisiae to identify its genetic partners and further elucidate its role during ribosomal biogenesis. Our screen revealed interactions with mutants affected at v...
Article
Full-text available
Telomere maintenance in eukaryotes is linked to cellular proliferation capacity. The telomerase holoenzyme, which maintains telomere length, is a central actor in this process and its function is highly conserved among eukaryotes. Telomerase is a ribonucleoproteic complex constituted of an RNA template and proteic subunits with catalytic and regula...
Article
Full-text available
The yeast telomerase holoenzyme, which adds telomeric repeats at the chromosome ends, is composed of the TLC1 RNA and the associated proteins Est1, Est2 and Est3. To study the biogenesis of telomerase in endogenous conditions, we performed fluorescent in situ hybridization on the native TLC1 RNA. We found that the telomerase RNA colocalizes with te...
Article
Le contrôle de la longueur des télomères est une étape critique régissant le potentiel réplicatif des cellules eucaryotes. A cause du problème de fin de réplication, les chromosomes raccourcissent à chaque cycle de division. Ce raccourcissement se produit dans des séquences particulières appelées télomères. La longueur des télomères est en relation...

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