Francisco Rivero

Francisco Rivero
University of Hull · Hull York Medical School (HYMS)

MD PhD FHEA

About

149
Publications
13,489
Reads
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4,396
Citations
Citations since 2017
18 Research Items
1196 Citations
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2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
Introduction
Dr Rivero is a molecular cell biologist who specialises in research into the cytoskeleton, covering basic functional and regulatory aspects (in particular Rho GTPase-regulated pathways), both in mammalian cells and lower eukaryotes. For more information visit http://frivero-crespo.wix.com/rivero-lab
Additional affiliations
June 2017 - present
University of Hull
Position
  • Professor (Full)
October 2007 - June 2017
The University of Hull
Position
  • Professor (Associate)
Description
  • Senior Lecturer in Biomedical Sciences
September 1997 - October 2007
Universität Köln
Education
October 1998 - November 2003
University of Cologne
Field of study
  • Molecular Cell Biology
January 1990 - April 1994
Complutense University of Madrid
Field of study
  • Biochemistry and Molecular Biology
September 1983 - July 1989
Universidad de Valladolid
Field of study
  • Medicine

Publications

Publications (149)
Article
Full-text available
The myosin light chain phosphatase target subunit 1 (MYPT1), encoded by the PPP1R12A gene, is a key component of the myosin light chain phosphatase (MLCP) protein complex. MYPT1 isoforms have been described as products of the cassette-type alternative splicing of exons E13, E14, E22, and E24. Through in silico analysis of the publicly available EST...
Article
Full-text available
Bacterial infections are a major cause of morbidity and mortality in chronic lymphocytic leukemia (CLL), and infection risk increases in patients treated with the Bruton’s tyrosine kinase (Btk) inhibitor, ibrutinib. Btk and related kinases (like Tec) are expressed in non-leukemic hematopoietic cells and can be targeted by ibrutinib. In platelets, i...
Article
Full-text available
The GTP binding proteins known as small GTPases make up one of the largest groups of regulatory proteins and control almost all functions of living cells. Their activity is under respectively positive and negative regulation by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs), which together with their upstream regul...
Article
Full-text available
The actin cytoskeleton is of fundamental importance for eukaryotic cell homeostasis [...]
Article
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Remodeling of the actin cytoskeleton is one of the critical events that allows platelets to undergo morphological and functional changes in response to receptor-mediated signaling cascades. Coronins are a family of evolutionarily conserved proteins implicated in the regulation of the actin cytoskeleton, represented by the abundant coronins 1, 2, an...
Article
Full-text available
Rapid reorganization of the actin cytoskeleton in response to receptor-mediated signaling cascades allows platelets to transition from a discoid shape to a flat spread shape upon adhesion to damaged vessel walls. Coronins are conserved regulators of the actin cytoskeleton turnover but they also participate in signaling events. To gain a better pict...
Article
Full-text available
Background: The inhibitory subunit of cardiac troponin (cTnI) is a gold standard cardiac biomarker and also an essential protein in cardiomyocyte excitation-contraction coupling. The interactions of cTnI with other proteins are fine-tuned by post-translational modification of cTnI. Mutations in cTnI can lead to hypertrophic cardiomyopathy. Method...
Article
Full-text available
Typical Rho GTPases, such as Rac1, Cdc42, and RhoA, act as molecular switches regulating various aspects of platelet cytoskeleton reorganization. The loss of these enzymes results in reduced platelet functionality. Atypical Rho GTPases of the RhoBTB subfamily are characterized by divergent domain architecture. One family member, RhoBTB3, is express...
Article
Full-text available
Glioblastomas (GBM) are the most common grade 4 brain tumours; patients have very poor prognosis with an average survival of 15 months after diagnosis. Novel research lines have begun to explore aberrant protein arginine methylation (ArgMe) as a possible therapeutic target in GBM and ArgMe inhibitors are currently in clinical trials. Enzymes known...
Chapter
The model organism D. discoideum is well suited to investigate basic questions of molecular and cell biology, particularly those related to the structure, regulation, and dynamics of the cytoskeleton, signal transduction, cell-cell adhesion, and development. D. discoideum cells make use of Rho-regulated signaling pathways to reorganize the actin cy...
Chapter
Platelets are a critical cell for prevention of bleeding. Part of the response to the formation of the thrombus is the activation of the actin cytoskeleton, with an inability to effectively activate the cytoskeleton linked to thrombus formation defects and instability. The control of this process is linked to activation of the Rho GTPases, Cdc42, R...
Article
Full-text available
Platelets undergo profound shape changes upon adhesion to damaged blood vessel walls that are mediated by reorganisation of the actin cytoskeleton in response to receptor-mediated signalling cascades. The highly conserved 56 kDa multidomain cyclase associated protein 1 (CAP1) works in concert with cofilin and profilin to modulate actin filament tur...
Chapter
Small GTPases of the Rho family are ubiquitous molecular switches involved in the regulation of most actin cytoskeleton dependent processes and many other processes not directly linked to actin. D. discoideum is a well-established model organism for studies of the actin cytoskeleton and its regulation by signal transduction pathways. D. discoideum...
Article
Full-text available
With their essential role in inner ear function, stereocilia of sensory hair cells demonstrate the importance of cellular actin protrusions. Actin packing in stereocilia is mediated by cross-linkers of the plastin, fascin, and espin families. Although mice lacking espin (ESPN) have no vestibular or auditory function, we found that mice that either...
Article
Full-text available
RhoBTB proteins constitute a subfamily of atypical Rho GTPases represented in mammals by RhoBTB1, RhoBTB2, and RhoBTB3. Their characteristic feature is a carboxyl terminal extension that harbors two BTB domains capable of assembling cullin 3-dependent ubiquitin ligase complexes. The expression of all three RHOBTB genes has been found reduced or abo...
Article
Full-text available
Hearing relies on the mechanosensory inner and outer hair cells of the organ of Corti, which convert mechanical deflections of their actin-rich stereociliary bundles into electrochemical signals. Several actin-associated proteins are essential for stereocilia formation and maintenance, and their absence leads to deafness. One of the most abundant a...
Article
Full-text available
RhoBTB3 is an atypical member of the Rho family of small GTPases. It localizes at the Golgi apparatus and endosomes and is involved in vesicle trafficking and in targeting proteins for degradation in the proteasome. Previous studies using Northern blot analysis showed that Rhobtb3 is ubiquitously expressed in adult mice, but expression is particula...
Article
Full-text available
Significance Mammalian nonmuscle myosin II, a major player in cell migration and polarity, is regulated through heavy chain phosphorylation. The signaling mechanisms remain largely unknown. We implicate here coronin in this pathway. Coronin has a Cdc42- and Rac-interactive binding (CRIB) motif with which it binds preferentially to the GDP-loaded fo...
Article
Large-scale gene expression analysis has been applied recently to uncover groups of genes that are co-regulated in particular processes. Here we undertake such an analysis on CAP, a protein that participates in the regulation of the actin cytoskeleton and in cAMP signaling in Dictyostelium. microarray analysis revealed that loss of CAP altered the...
Article
Los factores de crecimiento similares a insulina (igf-i y ii) tienen un papel relevante en la regulación del desarrollo en etapas de inmadurez. En esta tesis se presenta un estudio conjunto en etapa fetal y neonatal con el fin de establecer la importancia de los nutrientes y la insulina en la regulación de estos factores. Se emplean un modelo de su...
Article
Full-text available
Background Rap proteins belong to the Ras family of small G-proteins. Dictyostelium RapA is essential and implicated in processes throughout the life cycle. In early development and chemotaxis competent cells RapA induces pseudopod formation by activating PI3K and it regulates substrate attachment and myosin disassembly via the serine/threonine kin...
Book
Dictyostelium discoideum is a well-establish eukaryotic model organism that offers unique advantages for studying fundamental cellular processes, including signal transduction, random and directed cell motility, cytokinesis, endocytosis and vesicle transport and development. Dictyostelium is also increasingly used for the investigation of human dis...
Article
RhoBTB (BTB stands for broad-complex, tramtrack, bric à brac) proteins are tumor suppressors involved in the formation of cullin 3 (Cul3)-dependent ubiquitin ligase complexes. However, no substrates of RhoBTB-Cul3 ubiquitin ligase complexes have been identified. We identified MUF1 (LRRC41, leucine-rich repeat containing 41) as a potential interacti...
Article
Full-text available
Dock (dedicator of cytokinesis) proteins represent a family of guanine nucleotide exchange factors (GEFs) that include the well-studied Dock180 family and the poorly characterised zizimin family. Our current understanding of Dock180 function is that it regulates Rho small GTPases and thus has a role in a number of cell processes, including cell mig...
Article
Full-text available
Cell adhesion, an integral part of D. discoideum development, is important for morphogenesis and regulated gene expression in the multicellular context and is required to trigger cell-differentiation. G-protein linked adenylyl cyclase pathways are crucially involved and a mutant lacking the aggregation specific adenylyl cyclase ACA does not undergo...
Data
Figure S2. Redistribution of GFP-CAP or GFP-N-CAP during macropinocytosis is unaffected in aca-cells. (A) Live dynamics of GFP-CAP during macropinocytosis of aca-cells. Cells were allowed to adhere on coverslips and challenged with TRITC dextran (marker for pinocytosis) and series of images were obtained with confocal microscopy. GFP-CAP redistribu...
Data
Figure S3. Redistribution GFP-CAP proteins during phagocytosis is unaltered in the absence of ACA. (A) aca-cells and transformants expressing GFP-CAP or its domains were allowed to adhere onto coverslips and were exposed to unlabelled yeast cells for 15 min, fixed with methanol (-20°C) for 10 min and immunostained with CAP specific mAb 230-18-8. Th...
Data
Figure S1. Distribution of GFP-CAP or its domains is unaltered in aca- cells. The aca-cells expressing the GFP fusions of full length CAP or its N- or C-domains with or without the proline rich regions were fixed with methanol at -20°C for 10 min and immunolabeled with CAP specific mAb 230-18-8 which recognizes the GFP fusions of full length CAP, N...
Data
Full-text available
Figure S6. Expression of GFP-CAP or GFP-N-pro-CAP influenced the polarity defects of aca- cells. Aggregation competent aca- cells expressing GFP-CAP (A) or GFP-N-pro-CAP (B) were starved for 6 h, fixed with methanol at -20°C and immunostained with antibodies specific to myosin (56-395-2), α-actinin (47-62-2) and filamin (82-454-12). Confocal micros...
Data
Full-text available
Figure S7. Analysis of cAR1 expression in aca- cells expressing GFP-CAP or GFP-N-pro-CAP. Northern blot showing the levels of cAR1 mRNA were unaltered in aca- cells and cells expressing GFP-CAP or GFP-N-pro-CAP. AX2 served as control. The time of development is given in hours (h).
Data
Figure S4. Localization of myosin II, α-actinin and filamin in aca- cells. Aggregation competent AX2 and aca cells were starved for 6 h, fixed with methanol at -20°C and immunostained with antibodies specific to myosin (56-395-2), α-actinin (47-62-2) or filamin (82-454-12). Confocal microscopy showed myosin at posterior and lateral cell projections...
Data
Full-text available
Figure S5. Localization of GFP-CAP in AX2 cells. Aggregation competent AX2 cells expressing GFP-CAP were starved for 6 h, fixed with methanol at -20°C and immunostained with antibodies specific to myosin (56-395-2), α-actinin (47-62-2) and filamin (82-454-12). Confocal microscopy showed that GFP-CAP in AX2 cells colocalizes with myosin, α-actinin a...
Book
Although initially described as major regulators of cytoskeletal remodeling, Rho GTPases have been implicated in the establishment of polarity, endocytosis, vesicle trafficking, morphogenesis, cytokinesis, transcriptional activation, cell cycle progression, and apoptosis, to mention a few. In addition, Rho GTPases have acquired medical relevance be...
Article
The model organism D. discoideum is well suited to investigate basic questions of molecular and cell biology, particularly those related to the structure, regulation, and dynamics of the cytoskeleton, signal transduction, cell-cell adhesion, and development. D. discoideum makes use of Rho-regulated signaling pathways to reorganize its cytoskeleton...
Article
Full-text available
Actin-bundling proteins are identified as key players in the morphogenesis of thin membrane protrusions. Until now, functional redundancy among the actin-bundling proteins villin, espin, and plastin-1 has prevented definitive conclusions regarding their role in intestinal microvilli. We report that triple knockout mice lacking these microvillar act...
Article
Full-text available
Dictyostelium discoideum (DD), an extensively studied model organism for cell and developmental biology, belongs to the most derived group 4 of social amoebas, a clade of altruistic multicellular organisms. To understand genome evolution over long time periods and the genetic basis of social evolution, we sequenced the genomes of Dictyostelium fasc...
Article
Ras-related GTPases of the Miro family have been implicated in mitochondrial homeostasis and microtubule-dependent transport. They consist of two GTP-binding domains separated by calcium-binding motifs and of a C-terminal transmembrane domain that targets the protein to the outer mitochondrial membrane. We disrupted the single Miro-encoding gene in...
Data
Supplementary Tables S5. A table listing the predicted paralogs that are shared between D. discoideum and D. purpureum.
Data
Supplementary Table S4. A table listing the predicted orthologs that are shared between D. discoideum and D. purpureum.
Data
Supplementary Table S2. A table listing blocks of partially conserved gene order between the D. discoideum and D. purpureum genomes.
Data
Supplementary text, figures and tables. Supplementary text, figures and tables that include many details of the genome annotation.
Article
Full-text available
The social amoebae (Dictyostelia) are a diverse group of Amoebozoa that achieve multicellularity by aggregation and undergo morphogenesis into fruiting bodies with terminally differentiated spores and stalk cells. There are four groups of dictyostelids, with the most derived being a group that contains the model species Dictyostelium discoideum. We...
Data
A. Expression of GFP-GAPA rescues the cytokinesis defect in GAPA− cells. Nuclei are stained with DAPI. Bar, 10 µm. B. Vegetative wild type cells expressing GFP-GAPA were analysed using confocal microscopy. The Inset shows a fluorescence intensity profile of GFP-GAPA distribution in a representative cell (measured using Image J software) through an...
Article
Full-text available
Filamin and Cortexillin are F-actin crosslinking proteins in Dictyostelium discoideum allowing actin filaments to form three-dimensional networks. GAPA, an IQGAP related protein, is required for cytokinesis and localizes to the cleavage furrow during cytokinesis. Here we describe a novel interaction with Filamin which is required for cytokinesis an...
Conference Paper
Background / Purpose: Rho proteins have been implicated mainly in the regulation of the cytoskeleton, but also in vesicle trafficking, phagocytosis and transcriptional activation. RhoBTB proteins constitute a subfamily of atypical Rho GTPases represented by three isoforms in vertebrates. They consist of a GTPase domain followed by a proline-rich...
Article
Full-text available
We present an inventory of single or multiple calponin homology (CH) domain containing proteins of Dictyostelium discoideum. A multiple alignment and a phylogenetic tree of all 60 CH domains found in 36 proteins showed that most CH domains can be assigned to one of 6 types. We have then distributed the proteins into several classes according to the...
Article
Full-text available
All human pathogenic Yersinia species share a virulence-associated type III secretion system that translocates Yersinia effector proteins into host cells to counteract infection-induced signaling responses and prevent phagocytosis. Dictyostelium discoideum has been recently used to study the effects of bacterial virulence factors produced by intern...
Article
Full-text available
Plastin 1 (I-plastin, fimbrin) along with villin and espin is a prominent actin-bundling protein of the intestinal brush border microvilli. We demonstrate here that plastin 1 accumulates in the terminal web and interacts with keratin 19, possibly contributing to anchoring the rootlets to the keratin network. This prompted us to investigate the impo...
Article
RhoBTB proteins are atypical members of the Rho family of small GTPases. Two of the three RhoBTB proteins, RhoBTB1 and RhoBTB2, have been proposed as tumor suppressors and might function as adaptors of Cul3-dependent ubiquitin ligase complexes. Using yeast two-hybrid analysis and co-immunoprecipitation we show that all three RhoBTB proteins interac...
Article
Full-text available
Ras guanine nucleotide exchange factor (GEF) Q, a nucleotide exchange factor from Dictyostelium discoideum, is a 143-kD protein containing RasGEF domains and a DEP domain. We show that RasGEF Q can bind to F-actin, has the potential to form complexes with myosin heavy chain kinase (MHCK) A that contain active RasB, and is the predominant exchange f...
Article
The centrosome-nucleus attachment is a prerequisite for faithful chromosome segregation during mitosis. We addressed the function of the nuclear envelope (NE) protein Sun-1 in centrosome-nucleus connection and the maintenance of genome stability in Dictyostelium discoideum. We provide evidence that Sun-1 requires direct chromatin binding for its in...
Article
RhoBTB proteins constitute a subfamily of atypical members within the Rho family of small guanosine triphosphatases (GTPases). Their most salient feature is their domain architecture: a GTPase domain (in most cases, non-functional) is followed by a prolinerich region, a tandem of 2 broadcomplex, tramtrack, bric a brac (BTB) domains, and a conserved...