Francisco J Lara

Francisco J Lara
University of Granada | UGR · Department of Analytical Chemistry

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62
Publications
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Publications

Publications (62)
Chapter
Traditionally, capillary electrophoresis (CE) has been ruled out of many food safety applications, despite its inherent advantages, because its concentration sensitivity has been not high enough, mainly in relation to the monitoring of contaminants and residues, such as pesticides, veterinary medicines, environmental contaminants, toxins, etc. For...
Article
Full-text available
Polymeric nano- and microfibers were tested as potential sorbents for the extraction of five neonicotinoids from natural waters. Nanofibrous mats were prepared from polycaprolactone, polyvinylidene fluoride, polystyrene, polyamide 6, polyacrylonitrile, and polyimide, as well as microfibers of polyethylene, a polycaprolactone nano- and microfiber co...
Article
Full-text available
In this work, it is proposed for the first time an electrophoretic approach based on micellar electrokinetic chromatography coupled with tandem mass spectrometry (MEKC-MS/MS) for the simultaneous determination of nine neonicotinoids (NNIs) together with the fungicide boscalid in pollen and honeybee samples. The separation was performed using ammoni...
Article
The presence of cyanobacteria and cyanotoxins in all water bodies, including ocean water and fresh water sources, represents a risk for human health as eutrophication and climate change are enhancing their level of proliferation. For risk assessment and studies on occurrence, the development of reliable and sensitive analytical approaches able to c...
Article
Current trends in analytical chemistry encourage the use of innocuous solvents to develop modern methods aligned with green chemistry. In this sense, natural deep eutectic solvents (NADESs) have emerged as a novel generation of green solvents which can be employed in sample treatments as an alternative to the toxic organic solvents commonly used so...
Article
Capillary liquid chromatography (CLC) with UV-diode array detection has been proposed for the first time to determine fipronil and two metabolites, fipronil-sulfide and fipronil-sulfone in hen egg samples. Under optimum conditions, analytes eluted in less than 12 minutes. Despite the complexity of egg samples, a simple and fast sample preparation m...
Article
Full-text available
An ultra-high performance liquid chromatography coupled to tandem mass spectrometry method is proposed for the determination of the major ergot alkaloids (ergometrine, ergosine, ergotamine, ergocornine, ergokryptine, ergocristine) and their epimers (ergometrinine, ergosinine, ergotaminine, ergocorninine, ergokryptinine, and ergocristinine) in oat-b...
Article
A simple and effective method using solid-liquid extraction followed by capillary liquid chromatography with diode array detection (CLC-DAD) has been established for the extraction and determination of seven neonicotinoid insecticides commercially available (imidacloprid, thiacloprid, clothianidin, thiamethoxam, acetamiprid, nitenpyram and dinotefu...
Article
A new analytical method based on capillary LC with diode array detection has been developed for the simultaneous quantification of seven neonicotinoid insecticides commercially available (imidacloprid, thiacloprid, clothianidin, thiamethoxam, acetamiprid, nitenpyram and dinotefuran) in honey samples. The separation was achieved in a Zorbax XDB‐C18...
Article
The global concern about food safety and environment quality has produced the development of a legal framework to control residues of organic compounds (e.g. pesticides and veterinary and human drugs). In this context, the establishment of analytical methods achieving satisfactory performances in terms of sensitivity, selectivity, resolution, ident...
Article
Full-text available
A simple, sensitive, and efficient method has been developed for the determination of the seven neonicotinoid insecticides commercially available (imidacloprid, thiacloprid, clothianidin, thiamethoxam, acetamiprid, nitenpyram, and dinotefuran) and the main metabolite 6-chloronicotinic acid. Micellar electrokinetic chromatography (MEKC) mode was app...
Article
Fipronil is an insecticide that is not approved in the European Union in food. In 2017 fipronil was involved in a European health alert due to its presence in fresh hen eggs because of an illicit use in poultry farms, so reliable methods are needed to determine fipronil and its main metabolites in these matrixes. In this work, we report the first a...
Article
In this study two different analytical approaches have been developed to determine the presence of several cyanotoxins in saline water samples from a continental salt marsh. A salting-out assisted liquid-liquid extraction (SALLE) has been used in combination with ultra-high performance liquid chromatography-tandem mass spectrometry and UV-diode arr...
Article
In the context of human and veterinary drugs identification, ion mobility spectrometry (IMS) in combination with mass spectrometry (MS) may provide a relevant complementary piece of information to mass-to-charge ratio (m/z), the so-called collision-cross-section (CCS). Up to now, however, the application of CCS as identification parameter has not b...
Chapter
Food safety is related to the monitoring of hazards in food to protect the consumer health. In order to guarantee safe and good-quality food and to meet international trade requirements, the development of selective, sensitive, and accurate analytical methods is mandatory for the control of a great number of toxicants, including chemical hazards. I...
Article
A green and simple multiresidue method using capillary liquid chromatography (CLC) with UV-diode array detection (DAD) has been developed for the determination of sixteen benzimidazoles (BZs) and its metabolites in milk samples. The separation was achieved in <32 min, using a Zorbax XDB-C18 column (150 mm × 0.5 mm I.D, 5 μm), with a mobile phase co...
Article
Ultra-high performance liquid chromatography (UHPLC) coupled with fluorescence detection (FL) has been proposed for the first time to determine thirteen benzimidazoles (BZs) in farmed fish samples. In order to optimize the chromatographic separation, parameters such as mobile phase composition and flow rate were carefully studied, establishing a gr...
Chapter
In this article capillary electrophoresis (CE) is presented as a well-established separation technique, applied in food analysis because of its advantages related with the high separation efficiency, short analysis time and low cost. Its applications in the field of food analysis cover the determination of major compounds such as proteins, carbohyd...
Article
Abstract A new method based on micellar electrokinetic chromatography-tandem mass spectrometry (MEKC-MS/MS) has been developed for the identification and simultaneous quantification of thirteen benzimidazoles in animal urine. In order to obtain an appropriate separation with the highest sensitivity, different electrophoretic parameters were evaluat...
Article
A novel method based on capillary zone electrophoresis-tandem mass spectrometry has been proposed and validated for the identification and simultaneous quantification of twelve benzimidazoles in meat samples. Electrophoretic separation was carried out using 500 mM formic acid (pH 2.2) as background electrolyte and applying a voltage of 25 kV at 25...
Article
Direct analysis in real time (DART) was evaluated for the determination of a number of highly polar pesticides using the Quick Polar Pesticides Extraction (QuPPe) method. DART was hyphenated to high resolution mass spectrometry (HRMS) in order to get the required selectivity that allows the determination of these compounds in complex samples such a...
Article
A novel method based on capillary zone electrophoresis-tandem mass spectrometry has been proposed and validated for the identification and simultaneous quantification of twelve benzimidazoles in meat samples. Electrophoretic separation was carried out using 500 mM formic acid (pH 2.2) as background electrolyte and applying a voltage of 25 kV at 25°...
Chapter
The global concern about food safety and environment quality has produced the development of a legal framework to control residues of organic compounds (e.g. pesticides and veterinary and human drugs). In this context, the establishment of analytical methods achieving satisfactory performances in terms of sensitivity, selectivity, resolution, ident...
Article
Recently, chemiluminescence (CL)-based detection has become a very useful detection system in high-performance liquid chromatography (HPLC) due to its simplicity, low cost, high sensitivity and high selectivity. Some methods such as the use of photochemically induced CL reactions, the application of nanoparticles and new sample treatments have been...
Article
In this work molecularly imprinted polymers have been evaluated as sorbent for the construction of an in-line solid phase extraction analyte concentrator in capillary electrophoresis coupled with mass spectrometry for the determination of the eight regulated veterinary quinolones in bovine milk samples. Different parameters affecting the analyte co...
Article
Full-text available
Seven solid phase sorbent materials with reversed-phase, mixed-mode interactions (ion-exchange and reversed-phase), and molecularly imprinted polymers (MIP), namely Oasis HLB, Oasis MAX, Oasis MCX, Bond Elute Plexa, Bond Elute Plexa PAX, Bond Elute Plexa PCX, and SupelMIP sorbents, were investigated. The present study was focused on the retention a...
Article
A new simple analytical method for monitoring propyl propane thiosulfonate (PTSO) in animal feed is presented. PTSO is an active ingredient from Allium spp. (like onion and shallot), proposed as a natural additive for feed being an efficient alternative to antibiotics used as growth promoter due to its efficiency improving animal health. Reversed-p...
Article
Full-text available
In this study a simple, rapid, and efficient method has been developed for the determination of six sulfonylurea herbicides (SUHs): triasulfuron, metsulfuron-methyl, chlorsulfuron, flazasulfuron, chlorimuron-ethyl, and primisulfuron-methyl in commercial grape and apple juice samples, using dispersive liquid–liquid microextraction coupled with capil...
Article
A three-phase hollow-fiber liquid-phase microextraction combined with a capillary liquid chromatography method using diode array detection was proposed for the determination of six sulfonylurea herbicides, triasulfuron, metsulfuron-methyl, chlorsulfuron, flazasulfuron, chlorimuron-ethyl and primisulfuron-methyl, in environmental water samples. Diff...
Article
A three-phase hollow-fiber liquid-phase microextraction combined with a capillary LC method using diode array detection was proposed for the determination of six sulfonylurea herbicides, triasulfuron, metsulfuron-methyl, chlorsulfuron, flazasulfuron, chlorimuron-ethyl, and primisulfuron-methyl, in environmental water samples. Different factors that...
Article
Patulin (PAT) is a mycotoxin naturally found in fruits, including apples. Its occurrence as a natural contaminant of fruit juices is indicative of fruit quality in production. The European Union has set the maximum content of patulin in 50 μg kg−1 for fruit juices and 10 μg kg−1 for infant fruit juices. In this paper, dispersive liquid–liquid micro...
Article
β-lactam antibiotics have been the most widely used antimicrobial drugs for more than 80 years and still constitute the most important group of antibiotics. Their extensive use in veterinary medicine practices as growth promoters, and chemotherapeutic and/or prophylactic agents causes numerous residues in foodstuffs which present a serious health h...
Article
Gallium phosphide (GaP) PIN photodetectors have been flip-chip bonded onto glass substrates and then studied for their ability to detect capillary fluorescence. The system presents a compact, robust alternative to liquid chromatography and capillary electrophoresis. Linear fluorescence detection of Ciprofloxacin to concentrations of 0.01 ppm was ac...
Article
Full-text available
Ciprofloxacin is a commonly used antibiotic and the active ingredient in a veterinary antibiotic. Detecting its presence allows us to understand its absorption process in blood as well as tissue. A portable microfluidic system has been fabricated. It operates at low bias voltage and shows a linear relationship between concentration levels and syste...
Article
In this review, the recent evolution and the state of the art of photochemical reactions coupled with chemiluminescence processes are presented. Different chemiluminescence systems have been considered together with suitable photochemical derivatization processes that can affect either the analyte of interest or even the chemiluminogenic reagent, p...
Article
The present review presents the state of the art of the developments, key strategies and analytical applications of chemiluminescence detection coupled to CE (CE-CL). Different parts considering the most common CL systems have been included, such as the tris(2,2'-bipyridine)ruthenium(II) system, the luminol and derivatives reaction, the peroxyoxala...
Article
In recent years, chemiluminescence (CL)-based detection coupled to capillary electrophoresis (CE) as separation technique has attracted much interest due to new advances in home-made configurations, sample-treatment techniques for application to real matrixes, development of a commercial instrument and use of miniaturization techniques to obtain mi...
Article
Full-text available
In this paper we review applications of capillary electrophoresis (CE) to the determination of antibiotic residues in food derived from animals and in environmental samples. Although many CE methods have been used to determine antibiotics in the pharmaceutical field (drug quality control or therapeutic monitoring in biological samples), food and en...
Article
Chemiluminescence (CL)-based detection has become in the last years quite a useful detecting tool in liquid chromatography (HPLC) due to its simplicity, low cost and high sensitivity and selectivity, and the development in instrumentation. Minimal instrumentation is required and no external light source is needed; thus, the optical system is quite...
Article
An in-line solid-phase extraction-capillary electrophoresis method with UV-vis detection was developed for the monitoring of residues of five sulfonamides (sulfadoxin, sulfadimethoxine, sulfamerazine, sulfachloropyridazine and sulfamethoxazole) in tap, bottled mineral and river waters. For this purpose an analyte concentrator was constructed, based...
Article
Molecularly imprinted polymers (MIPs) have been evaluated as sorbent for the construction of an in-line solid phase extraction concentrator in capillary electrophoresis to be applied in the monitoring of triazine herbicides: atrazine and its three metabolites, desethylatrazine, desisopropylatrazine and desethyldesisopropylatrazine. Initially, the e...
Article
We have developed and validated a CE-MS/MS method using an in-line SPE device (analyte concentrator, AC) to determine eight quinolones of veterinary use whose maximum residue levels in animal edible tissues are established by the EU Council Regulation 2377/90, i.e., danofloxacin, sarafloxacin, ciprofloxacin, marbofloxacin, enrofloxacin, difloxacin,...
Article
The different chemiluminescence (CL) have been used for different analytical purposes for determination of catalysts, oxidants, inhibitors or substances. Important applications have been performed using CL detection coupled with flow injection analysis (FIA), mainly for quality control in pharmaceutical analysis. Many applications of FIA-CL involve...
Article
A new analytical method based on capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) is proposed and validated for the identification and simultaneous quantification of eight quinolones for veterinary use in bovine raw milk. The studied quinolones include danofloxacin, sarafloxacin, ciprofloxacin, marbofloxacin, enrofloxacin, diflox...
Article
A CE instrument coupled with chemiluminescence (CL) detection was designed for the determination of promethazine hydrochloride (PTH) and promazine hydrochloride (PMH) in real samples. An important enhancement of the CL emission of luminol with potassium ferricyanide was observed in the presence of these phenothiazines; so this system was selected f...
Article
A capillary zone electrophoresis (CZE) method with ultraviolet-visible detection has been established and validated for the determination of five phenothiazines: thiazinamium methylsulfate, promazine hydrochloride, chlorpromazine hydrochloride, thioridazine hydrochloride, and promethazine hydrochloride in human urine. Optimum separation was obtaine...
Article
We present the validation of a developed method using capillary zone electrophoresis (CZE) for quantitative analysis of three phenothiazines: thiazinamium methylsulphate (TMS), promazine hydrochloride (PMH) and promethazine hydrochloride (PTH) in pharmaceutical formulations. The influence of various parameters affecting the CZE separation (buffer p...

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Goal: This Special Issue presents the latest advances in the analysis of drug metabolites in various fields, addressing the development of drugs for medical purposes, doping control, new drugs of abuse, etc. Within this framework, this Special Issue covers recent analytical methods focused on the determination of metabolites of pharmaceutical, abuse and/or performance-enhancing drugs in biological samples from in vitro or in vivo studies. The scope is broad, and the submission of research papers and reviews covering those analytical methods based, among others, on chromatographic and related separation techniques (e.g., capillary electrophoresis), mass spectrometry and hyphenated techniques (e.g., ion mobility spectrometry), spectroscopic techniques, or biosensors is encouraged. New sample preparation protocols are also of special interest, since the extraction of metabolites in complex biological samples is one of the main challenges of the metabolite analysis. Finally, this Special Issue also includes metabolomics approaches as a novel strategy to study the mode of action of drugs, which has an impact on the development of pharmacologically analogous drugs, or to discover the consumption of novel abuse drugs not previously reported, but showing an identical or similar mode of action of known abuse drugs. Deadline for manuscript submissions: 20 June 2022. More information on this Special Issue can be found at: https://www.mdpi.com/journal/separations/special_issues/trends_identifying_drug_metabolites