Francisco Kuhar

Francisco Kuhar
INNOMY Biotech · Mycelium

PhD
CONICET associate researcher

About

70
Publications
80,771
Reads
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597
Citations
Citations since 2016
45 Research Items
545 Citations
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
2016201720182019202020212022020406080100120
Introduction
Mycologist specialized in fungal diversity (gasteroid and ectomycorrhizal fungi) and biotechnological applications of fungal enzymes with special interest in evolution of the sequestrate forms. Associate researcher at CONICET in the Instituto Multidisciplinario de Biologia Vegetal (IMBIV - UNC) and curator of Fungi at the CORD Herbarium.
Additional affiliations
April 2013 - May 2016
National Scientific and Technical Research Council
Position
  • PostDoc Position
Description
  • Morphotyping, matching and ecology of ectomycorrhiza in Nothofagus pumilio forest
April 2013 - September 2016
National Scientific and Technical Research Council
Position
  • Researcher
February 2010 - April 2013
Universidad de Buenos Aires
Position
  • Research Assistant

Publications

Publications (70)
Article
Full-text available
Patagonian collections of the corticioid genus Tomentellopsis have been treated in the past as T. echinospora, a common northern hemisphere species. New collections with DNA sequence data are distinct from the northern hemisphere taxon and must be considered a different species, endemic to the temperate subantarctic forests of Patagonia. We use mol...
Data
Mycelium materials based on Patagonian fungal strains have shown interesting mechanical properties that we share in this paper. We also show the relation between these properties and the hyphal structure of the mycelium.
Article
Full-text available
Mycelium composites consist of particulate lignocellulosic materials, e.g., sawdust from the timber industry structured as a solid matrix resulting from the mycelial growth. Many protocols have been proposed based on different strains and substrates. However, the influence of intrinsic elements, such as the structure of the hyphal system on the mai...
Article
Full-text available
Fungi show a high degree of morphological convergence. Regarded for a long time as an obstacle for phylogenetic studies, homoplasy has also been proposed as a source of information about underlying morphogenetic patterning mechanisms. The "local-activation and long-range inhibition principle" (LALIP), underlying the famous reaction-diffusion model...
Article
Full-text available
Mushrooms in the genus Ganoderma have been collected and used as medicine since ancient times. However, commercial basidiome production has only recently been achieved. The solid substrates for basidiome production usually consist of lignocellulosic materials as the major component and the supplements (e.g., different types of bran and flour) as th...
Article
Full-text available
Lysurus fossatii, a new Lysuraceae species with unique macroscopic features is described and illustrated. Specimens were collected during field expeditions to remnants of the Espinal forest preserved at Estancia Yucat, South-East Córdoba, in central Argentina. The species is diagnosed by a light yellow peridium when immature, with the surface marke...
Article
Full-text available
Las reconstrucciones de parentesco basadas en ADN demostraron que los Gasteromycetes descendían de formas himeniales. Pero eso no es lo extraño. Lo que más nos llama la atención, es que estos hongos con gleba no evolucionaron como un linaje, sino que aparecieron evolutivamente muchas veces.Algunos son parientes de champignones, otros, de parasoles,...
Article
In the Patagonian region, Cortinarius is the most diverse and abundant genus of ectomycorrhizal fungi with at least 250 species. Sequestrate forms were until recently documented within the genus Thaxterogaster, a genus now known to be polyphyletic, and many were consequently transferred to Cortinarius. Original descriptions were mostly available in...
Article
Full-text available
Fungi associated with leaf-cutter ants (LCAs) play a fundamental role in Neotropical ecosystems. The association with fungi allows LCAs to be the dominant herbivores and in this way influence ecological processes. In order to know the taxonomic diversity of fungal symbionts associated with LCAs, ten sampling sites were selected corresponding to the...
Research
Full-text available
Una narración con curiosas observaciones sobre los nombre de algunas especies de plantas, su origen, y la posible relación con sus propiedades.
Article
Collections of the genus Bovista in southern South America have been traditionally identified under northern-hemisphere names. Although many puffball species show a wide distribution , the identity and distribution of Patagonian species still need to be revisited. Recent materials collected in Patagonia and displaying a unique capillitium ornamente...
Book
Full-text available
Crónicas del reino de los hongos brinda respuestas a cientos de preguntas que nos hacemos y que recibimos a diario. Escritos de manera entretenida y lúdica, llenos de pasión por los hongos, cada capítulo deja entrever las virtudes únicas de los autores Kuhar, Romano, Grassi y Sequeira. Se unen el rigor de destacados científicos, el asombro propio d...
Technical Report
This species is only known from forest in the southern Yungas of Argentina, but research needs to be done to see whether it is present in Bolivia too. This habitat is deteriorating outside of reserves, and the population is thought to be in decline. Overall the population size is thought to be small (c.160 mature individuals), and so the species is...
Technical Report
This species has a restricted range in the Polylepis australis forest of the Cordoba High Mountains of Argentina. It is only known from two sites, and the overall population size is estimated to be very small (100-200 mature individuals) and in decline. Therefore, this species is listed as Critically Endangered.
Technical Report
There are currently only a limited number of known subpopulations of this species over a rather large geographic area in South America. The type locality is now possibly extinct, but further subpopulations potentially are yet to be discovered. The overall population size is estimated to be fewer than 1,000 mature individuals, and this species is li...
Technical Report
Currently fruiting bodies of this species have only been recorded from southern Patagonia. Recent root tip analysis (Tedersoo and Smith 2013) has shown that this species occurs in northern Patagonia too, but there have been no known collections of fruiting bodies in this area yet. Excluding lineages from Tedersoo and Smith (2013) that come from spe...
Article
Full-text available
Background: Pleurotus spp. are fungi characterized by their ability to biologically degrade lignocellulosic materials. In Argentina, 6 wild Pleurotus species are known, being P. pulmonarius and P. ostreatus the ones with the greatest bio­ technological interest. Objective: To determine the phylogenetic relationships of Pleurotus strains from Patag...
Article
Psathyloma is an agaricoid genus described recently to accommodate two species of brown-spored agarics from Australasia superficially similar to Psathyrella and Hebeloma. Phylogenetic analyses published along with the description of the genus determined that an unidentified species of Psathyloma was also present in Patagonia based on ITS sequences...
Article
Full-text available
El cultivo y consumo de hongos comestibles ha estado en aumento en los últimos años. Existe una demanda no satisfecha de este producto que hoy en día es necesario cubrir. En comparación con otros países, Argentina no tiene ligado a su historia el consumo de especies silvestres. A pesar de que los hongos cultivables más consumidos son los champiñone...
Chapter
This chapter compiles data on various aspects of ectomycorrhizal fungal taxa research, carried out in South America (SA) since the first studies in the early 1900. Early research was achieved by foreign mycologists and the development of the first biological research centers in the region. The diversity of fungal taxa and data on the associated pla...
Article
Because of systematic sampling campaigns in the northern Patagonian Nothofagaceae forests of Argentina, several specimens of sequestrate fungi were collected. Some of those collections showed phylogenetic affinities and morphological similarities to members of the formerly recognized sequestrate genus Thaxterogaster, currently a synonym of Cortinar...
Chapter
Full-text available
Cuando pensamos en los hongos lo primero que se nos viene a la mente es la imagen de las típicas setas con sombrero o las repisas que crecen sobre el tronco de los árboles. Sin embargo, estas imágenes sólo representan la estructura reproductiva de algunos hongos. El verdadero cuerpo de los hongos consiste en células individuales u organizadas a mod...
Article
Molecular phylogenetic analyses have addressed the systematic position of several major Northern Hemisphere lineages of Pezizales but the taxa of the Southern Hemisphere remain understudied. This study focuses on the molecular systematics and taxonomy of Southern Hemisphere species currently treated in the genera Underwoodia and Gymnohydnotrya . Sp...
Article
Full-text available
El advenimiento de la microscopía óptica produjo una revolución en las ciencias naturales, con su mayor impacto en el estudio de los seres vivos. La capacidad de distinguir las mínimas unidades de la vida reveló la presencia de grupos de organismos insospechados hasta entonces y comenzó a mostrar las increíbles formas de reproducirse y dispersarse...
Article
Full-text available
As public policies and conservation requirements for biodiversity evolve there is a need for a term for the kingdom Fungi equivalent to Fauna and Flora. This need is considered to be urgent in order to simplify projects oriented toward implemention of educational and conservation goals. In an informal meeting held during the IX Congreso Latinoameri...
Method
Full-text available
El advenimiento de la microscopía óptica produjo una revolución en las ciencias naturales, con su mayor impacto en el estudio de los seres vivos. La capacidad de distinguir las mínimas unidades de la vida reveló la presencia de grupos de organismos insospechados hasta entonces y comenzó a mostrar las increíbles formas de reproducirse y dispersarse...
Article
Full-text available
Nomenclatural type definitions are one of the most important concepts in biological nomenclature. Being physical objects that can be re-studied by other researchers, types permanently link taxonomy (an artificial agreement to classify biological diversity) with nomenclature (an artificial agreement to name biological diversity). Two proposals to am...
Article
Thelephoroid fungi are abundant ectotrophic symbionts in temperate forests worldwide. The state of Sikkim, in India, constitutes a hot spot of fungal diversity due to the occurrence of many ectomycorrhizal tree species. Collections of a stipitate thelephoroid species were found under Castanopsis hystrix in the district South of this state. Molecula...
Article
Full-text available
Solid-state fermentation (SSF) with the medicinal higher Basidiomycete Ganoderma lucidum was studied as a strategy to use pine (Pinus radiata D. Don) and poplar (Populus nigra L.) wood chips and sawdust. Fruiting bodies were produced and the value of the biotransformed substrate was assessed. The highest mushroom yield (63 g dry weight per kilogram...
Article
Full-text available
Tomentella (Thelephorales, Basidiomycota) in Nothofagaceae forests of Patagonia, Argentina: mycorrhizae of new species. Tomentella is a mycorrhizal genus whose presence in temperate, tropical and circumpolar forests has been widely reported. It typically forms ectomycorrhizae (EM), but also orchidioid, ericoid, monotropoid and arbutoid symbiosis. T...
Conference Paper
Full-text available
Evolution of traits associated to sequestration in basidiomycetes Francisco Kuhar Centro de Investigación y Extensión Forestal Andino Patagónico (CONICET) fkuhar@gmail.com The set of features associated with the sequestration process within basidiomycetes has called the attention of many mycologists in the past and has been referred to as the " sec...
Article
Full-text available
Inocybe sindonia (Fr.) P. Karst. y Amanita rubescens Pers. son especies típicamente asociadas a bosques de Pseudotsuga menziesii (Mirb.) Franco y Pinus radiata D. Don respectivamente. Se reporta por primera vez su presencia en Argentina en bosques de Nothofagus de la Patagonia andina como resultado de la invasión de especies forestales introducidas...
Poster
Full-text available
Representatives of the family Thelephoraceae have not been studied in depth in Andean forests due to their inconspicuous fruiting bodies of relatively low abundance. Nothofagaceae-dominated ecosystems constitute one of the most extensive ectomycorrhizal forest types in the southern hemisphere. Growing evidence suggests that many Thelephoraceae spec...
Article
Full-text available
The descolea clade includes species of ectomycorrhizal basidiomycetes in the genera Descolea, Setchelliogaster, Descomyces, and Timgrovea that are known primarily from the Southern Hemisphere. Taxa in this group produce basidiomes that range in morphology from typical epigeous mushrooms (Descolea) and secotioid taxa (Setchelliogaster) to fully gast...
Article
Full-text available
Amanita is a diverse and cosmopolitan genus of ectomycorrhizal fungi. Although most species fruit aboveground, some sequestrate taxa have been documented from Australia and the Mediterranean region. We describe A. nouhrae sp. nov., a new hypogeous (‘truffle-like’) species associated with Nothofagus antarctica in northern Patagonia. This constitutes...
Article
Full-text available
The VIIIth Brazilian Mycological Congress, held in Florianópolis (Brazil) in October 2016, was organized by the Universidade Federal de Santa Catarina and brought together more than 1000 scientists of 20 nationalities, which illustrates the dynamism of South American mycology, in many fields. One of the major take-home messages, amongst many resear...
Article
Full-text available
Identificación del oídio causado por Erysiphe trifoliorum (Wallr.) U. Braun en Melilotus officinalis. Identification of the powdery mildew caused by Erysiphe trifoliorum (Wallr.) U. Braun on Melilotus officinalis.
Article
Full-text available
Knowledge regarding the enzymatic machinery of fungi is decisive to understand their ecological role. The species of the genus Geastrum are known to grow extremely slowly in pure culture, which makes it difficult to evaluate physiological parameters such as enzyme activity. Qualitative assays were performed on isolates of four species of this genus...
Article
Full-text available
Molecular identification of powdery mildews is challenging due to the impossibility of processing sterile mycelium or cultures. For this reason, inexpensive and fast methods based on conidial germination are still a very reliable alternative for identifications at the species level. Melilotus officinalis is a very extended forage crop in the centra...
Poster
Full-text available
Ectomycorrhizae are mutualistic root symbionts of many trees, including Nothofagus, that facilitate the mobilization of essential nutrients to the plant. In return, plants provide the fungi with sugar produced via photosynthesis. The transoceanic distribution of Nothofagus forests in the Southern hemisphere represents an example of isolation by con...
Article
Full-text available
The genusTomentella forms abundant ectomycorrhizae in coniferous and deciduous forests worldwide. Molecular identification of root tips suggests undescribed species in the Nothofagus forests of Patagonia, Argentina. Tomentella tenuissima, T. pulvinulata and T. patagonica are described here as new to science based on morphological and molecular anal...
Poster
Full-text available
ECM morphotypes were separated from whole root systems of 600 juvenile plants extracted from Nothofagus pumilio forests from three locations of the province Chubut:Laguna Villarino (Villa), Lago Baggilt (Bagg) and Cañadón Huemules (Huem) during autumn and spring of 2013 and 2014. Morpho-anatomical and histochemical comparisons were performed and id...
Article
Full-text available
An expanded-polystyrene factory located in northern Buenos Aires reported unusual dark spots causing esthetic damage in their production. A fungal strain forming black-olive colonies on extract malt agar medium was isolated from the damaged material and identified as Aureobasidium pullullans var. melanogenum. This fungus is particularly known for i...
Article
Full-text available
Rugolo Maximiliano; Francisco Kuhar. 2014. " Brief notes on three gaste-roid fungi in the Andean Patagonia ". Lilloa 51 (1). In reviewing the entire collection of the ge-nus Geastrum of the BAFC herbarium, two samples were found containing unidentified specimens from Patagonia, one from Los Alerces National Park (Argentina, Chubut) and the other on...
Article
Full-text available
Ganoderma lucidum (Curtis) P. Karst is a white rot fungus that is able to degrade the lignin component in wood. The ability of two strains of this species to produce the ligninolytic enzyme laccase was assessed. After the evaluation of induction with heavy metals and phenolic compounds, it was found that among the tested substances, copper and feru...
Thesis
Full-text available
Ganoderma lucidum (Curtis) P. Karst es un hongo causante de pudrición blanca y por consiguiente es capaz de degradar la lignina de la madera y otros sustratos sobre los que crece. Se evaluó su capacidad de producir las enzimas ligninolíticas lacasa, manganeso peroxidasa y lignina peroxidasa, encontrándose que sólo la lacasa se produce con una activ...
Article
Full-text available
Ganoderma lucidum (Curtis) P. Karst es un hongo causante de pudrición blanca, capaz de degradar la lignina de la madera y otros sustratos en los que crece. En este trabajo se evaluó la capacidad de dos cepas de esta especie de producir la enzima ligninolítica lacasa. Asimismo, se ensayó la inducción de esta enzima con diferentes compuestos fenólico...
Poster
Full-text available
El nonil fenol (NP) es un resultante de la degradación de sus etoxilatos, utilizados por la industria como surfactantes. Su actividad estrogénica ha sido reportada numerosas veces. Dada su estructura aromática su degradación por ligninasas es una alternativa promisoria. Los objetivos de este trabajo son: 1) Proveer un ensayo sencillo, económico y r...
Poster
Full-text available
La degradación de colorantes trifenilmetánicos con cepas fúngicas se ha centrado tradicionalmente en hongos de la pudrición blanca, debido a su maquinaria enzimática oxidativa. Recientemente se han reportado actividades enzimáticas ligninolíticas en hongos de otros grupos ecológicos, que estarían asociadas a procesos como la interacción o la pigmen...
Article
Geastrum argentinum, a long forgotten species described by Spegazzini and previously regarded by Soto and Wright (2000) as a synonym of G. fimbriatum, has been found in several locations in Argentina. A careful analysis revealed relevant differences between those taxa, proving the validity of Spegazzini's species. Here we re-describe Spegazzini's s...
Article
Full-text available
La Micología es una disciplina científica cuyo objeto de estudio son los hongos; las investigaciones pueden ser encaradas desde la fisiología, taxonomía, biogeografía, etc. Para llevar adelante cualquiera de estos estudios los micólogos necesitan trabajar con cultivos puros de hongos y además con materiales de herbario. Por lo tanto el primer paso...
Article
Full-text available
Con el fin de obtener cepas fúngicas aptas para la biodegradación de colorantes trifenilmetánicos usando residuos de la producción maderera, se evaluó la capacidad de degradación de los colorantes verde de malaquita y violeta de genciana, por parte de sistemas consistentes en viruta de pino de producción local como medio de cultivo, y aislamientos...
Data
Full-text available
La Micología es una disciplina científica cuyo objeto de estudio son los hongos; las investigaciones pueden ser encaradas desde la fisiología, taxonomía, biogeografía, etc. Para llevar adelante cualquiera de estos estudios los micólogos necesitan trabajar con cultivos puros de hongos y además con materiales de herbario. Por lo tanto el primer paso...
Thesis
Full-text available
Ganoderma lucidum (Curtis) P. Karst is a white rot fungus and therefore is able to degrade the lignin of wood and other substrates on which it grows. The ability to produce the ligninolytic enzymes laccase, lignin peroxidase and manganese peroxidase were assessed. It was found that only laccase occurs with appreciable activity. Induction with heavy...
Article
Full-text available
En este artículo solo trataremos dos grandes grupos de hongos: Ascomycota y Basidiomycota, y hablaremos un poco de los distintos “tipos” que podemos encontrar dentro de estos dos grupos basándonos en la morfología de sus fructificaciones. El objetivo es orientar un poco a aquellas personas que se encuentran con un hongo en la naturaleza y quieren d...
Article
Full-text available
A survey of gasteroid fungi from arid regions in the La Rioja province (northwestern Argentina) resulted in 20 species of 13 genera. Each species is briefly commented on and descriptions are provided for taxa poorly known or with few records in the world. In addition, photographs of basidiomata and SEM micrographs illustrating basidiospores are pro...
Article
Full-text available
A survey of Geastrum species from arid lands in the La Rioja province (northwestern Argentina) recovered fourteen species previously unreported from the province: G. arenarium, G. campestre var. famatinum, G. corollinum, G. fimbriatum, G. floriforme, G. glaucescens, G. hieronymi, G. aff. lloydianum, G. pampeanum, G. pampeanum var. pallidum, G. parv...
Poster
Full-text available
La remoción de los restos leñosos de la tala comercial de árboles constituye un problema considerable para la silvicultura debido a los costos que implica esta tarea. El objetivo del trabajo es emplear hongos lignocelulolíticos, responsables naturales de la degradación de la madera, para acelerar la descomposición de los tocones a un período estima...
Poster
Full-text available
Ganoderma lucidum (russulales) es un hongo empleado por la medicina tradicional oriental desde antes de nuestra era. En la actualidad se le han reconocido nuevas aplicaciones en areas tales como medicina, biotecnología, biorremediación entre otras. Muchas sustancias aromáticas son reconocidos inductores de enzimas ligninolíticas. Al ensayar una bat...
Article
Full-text available
The aim of a joint effort by different research teams was to provide an improved procedure for enzyme activity profiling of field-sampled ectomycorrhizae, including recommendations on the best conditions and maximum duration for storage of ectomycorrhizal samples. A more simplified and efficient protocol compared to formerly published procedures wa...
Poster
Full-text available
Ganoderma lucidum y Trametes versicolor son dos hongos causantes de pudrición blanca en maderas debido a que su maquinaria enzimática oxidativa les permite degradar eficientemente la lignina. Dado que se trata de reconocidos productores de la enzima lacasa, se ensayó el cocultivo en distintos medios con el fin de incrementar esta actividad. Los ens...
Data
Logotype of HONGOS·AR - Hongos de Argentina. A multimedia website to learn all about Fungi! Visit us at www.hongosdeargentina.org or at www.hongosdeargentina.com.ar *The three authors equally contribute to HONGOS·AR project
Poster
Full-text available
El género Geastrum perteneciente al orden Geastrales se caracteriza por un exoperidio que se rompe en lacinias (radios) a modo de brazos de estrella dejando al descubierto el endoperidio globoso que libera las esporas por un poro ap ica l. Este género ha s ido poco estud iado en la provincia de La Rio ja (Argentina) caracterizada por un clima semiá...