Francisco Javier Deive Herva

Francisco Javier Deive Herva
University of Vigo | UVIGO · Department of Chemical Engineering

PhD Chemical Engineering

About

133
Publications
16,635
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2,495
Citations
Additional affiliations
January 2011 - August 2011
University of Santiago de Compostela
Position
  • Extraction of natural antioxidants
January 2009 - December 2010
Universidade NOVA de Lisboa
Position
  • Study of neoteric solvents for applications in biotechnology
September 2004 - December 2004
Technische Universität Hamburg
Position
  • Isolation of extremophiles for the production of metabolites with industrial interest

Publications

Publications (133)
Article
Full-text available
In this work, we report the CO 2 hydrate phase equilibria in water (H 2 O), heavy water (D 2 O), and their binary mixtures following the isochoric pressure search method using a rocking cell apparatus. The phase behaviour was mapped within the temperature and pressure range of 276.32 – 284.80 K and 1.59 – 3.78 MPa, respectively. It was found that t...
Article
In this study, a physical characterization (density (ρ), refractive index (nD), speed of sound (u) and dynamic viscosity (η)) as a function of temperature (298.15 to 343.15 K) of choline glycinate (ChGly) and its binary mixtures with water or/and methanol over the entire composition range was performed from 298.15 K to 323.15 K. Several derived pro...
Article
In this work, biocompatible ionic liquids based on aminoacids were employed as extractants to separate extremolipases from aqueous streams. First, the influence of aminoacid and dipeptide-based ionic liquids (cholinium glycinate, ChGly, and cholinium glycylglycinate, ChGlygly) on the lipolytic activity of a commercial lipase from Candida antarctica...
Article
The use of aqueous solutions of non-ionic surfactants like Tergitol 15-S7 and Tergitol 15-S9 is proposed for the first time to ex-situ remediation of Phenanthrene, Pyrene and Benzo[a]anthracene polluted soils, thus generating aqueous Polycyclic Aromatic Hydrocarbons (PAH)-polluted effluents. The effectiveness of several potassium based-inorganic sa...
Article
The present work demonstrates the suitability of a natural deep eutectic solvent (DES) composed of glucose and cholinium chloride to act both as lipase extractant and biodiesel enhancer. After having demonstrated the viability of using this DES as segregation agent in aqueous solutions of non-ionic surfactants widely employed as lipase inducers in...
Article
In the present work, the ability of different sodium-based salts like Na2CO3, Na2HPO4, Na2SO4 and Na2SO3 to salt out aqueous solutions of two biodegradable non-ionic surfactants (Tergitol 15S7 and Tergitol 15S9) has been demonstrated. We have empirically characterized the immiscibility regions and models like Merchuk and Othmer-Tobias equations wer...
Article
The main objective of the present work was to synthesize and characterize three novel lipase-friendly deep eutectic solvents (DESs) using cholinium dihydrogen phosphate (N1112OHDHP) as hydrogen bond acceptor and ethylene glycol (EG) and/or glycerol (GL) as hydrogen bond donors in the following proportions: N1112OHDHP:EG (molar ratio 1:2), N1112OHDH...
Article
The search of competitive extraction tools for extraction and containment of the enzymes biotechnologically produced in aqueous media is one appealing option to be investigated in transesterification biocatalysis. In this work, the use of a natural deep eutectic solvent (DES) composed of cholinium chloride and glucose has been demonstrated to be a...
Article
In this work, we have demonstrated the suitability of a novel Deep Eutectic Solvent (DES) composed of cholinium dihydrogen phosphate and ethylene glycol at a molar ratio of 1:2 (ChDHP:EG) to extract Candida antarctica lipase B from aqueous solutions of non-ionic surfactants Triton X-100 and Tween 80. The binodal curves were firstly explored at temp...
Article
This work is focused on the demonstration of the ability of DES composed of choline chloride (ChCl) as hydrogen bond acceptor (HBA) and urea and/or glycerol as hydrogen bond donors (HBD) to be employed as biocatalytic milieu in Candida antarctica lipase B (CALB)-catalysed biodiesel production. Not only, the biocatalytic activity was maintained in t...
Article
Full-text available
Efficient analytical methods are required for optimizing dosage of veterinary antibiotics and hormones in order to reduce toxicity and antimicrobial resistance in the environment. Thus, the objective of this work was to develop a rapid and low-cost method for determination of hormone estradiol and antibiotic chlortetracycline in bovine and porcine...
Article
In this work, a new family of ILs is here firstly presented, built with the biocompatible and biodegradable choline cation and oligopeptide-based anion. The simplest tripeptide triglycine (Gly-Gly-Gly) was employed for conforming a new choline tripeptide (Ch3Pe) IL, which sets the foundations for novel families of ILs based on peptides. Aiming at e...
Article
The segregation capacity of cholinium bitartrate (N1112OHC4H5O6) in aqueous solutions of biodegradable non-ionic surfactants belonging to Triton family (Triton X-100 and Triton X-102) has been firstly addressed. The influence of temperature on the separation potential was analysed by carrying out the binodal curves and tie-lines from 293.15 K to 32...
Article
This work bets in Choline (Ch) Amino Acid (AA)-based Ionic Liquids (ILs) to design a biocompatible separation process. This family represents a greener alternative regarding more toxic and recalcitrant analogues, like conventional imidazolium ILs. In this vein, the library of Ch-based ILs has been rationally incremented by utilizing L-Phenylalanyl-...
Article
This work is the first approach to extract extremozymes from aqueous media containing non-ionic surfactants. A biocompatible ionic liquid like cholinium glycinate ([N1112OH][Gly]) has been selected as phase forming agent in aqueous solutions of Triton X surfactants at several temperatures. Then, two in-house synthesized extremophilic lipolytic enzy...
Article
The generalized use of antibiotics and hormones in swine feed has triggered a growing concern on their presence in wastewater effluents and manure. Therefore, the implementation of strategies for their removal is a matter of practical significance and the synthesis of biocompatible ionic liquids to be used as segregation agents in aqueous waste eff...
Article
The growing social concern regarding the use of more environmentally friendly processes is compelling the search of more biocompatible solvents able to overcome the drawbacks associated to the conventional ones. Thus, in this work, a Deep Eutectic Solvent (cholinium chloride:urea) was demonstrated to act as salting out agent in aqueous solutions of...
Article
This work presents the first in‐depth study of Aqueous Triphasic Systems (A3PS) with the main purpose of unveiling their behaviour, hence contributing to the development of this new field. Thus, a complete definition of a quaternary system was carried through by describing all the regions in detail to later on represent them in a regular‐tetrahedra...
Article
The aqueous two-phase systems composed of the non-ionic surfactant Tergitol NP10 and the ionic liquids 1-ethyl-3-methyl imidazolium methylsulfate or 1-ethyl-3-methyl imidazolium ethylsulfate (C2C1imC1SO4 or C2C1imC2SO4) were characterized by the cloud point method. First, the binodal curves were determined and compared with the data reported previo...
Article
Cholinium bitartrate (N 1112OH C 4 H 5 O 6 ) has been demonstrated to be a promising phase disengagement agent in aqueous solutions of biodegradable non-ionic surfactants belonging to Tween family. For the first time, these systems were demonstrated to be versatile tools to efficiently extract two of the most frequently used groups of dyes, like di...
Article
The segregation capacity of cholinium dihydrogen phosphate (N 1112OH DHP) in aqueous solutions of two non-ionic surfactants belonging to Triton X family (Triton X-100 and Triton X-102) was explored at different temperatures from T = (298.15 to 333.15) K. The experimental data for each binodal curve have been correlated using three empirical models...
Article
The research on biocompatible ionic liquids is triggering a great interest in cholinium-based solvents, due to their alleged biocompatibility and biodegradability. However, the screening for choline degraders among different microbial sources from extreme locations revealed the suitability of just one halophilic strain (Halomonas sp. LM1C) to effic...
Article
Brewery spent grain (BSG), a relevant waste from beer industry mainly composed of polysaccharides and lignin, is experiencing a surge in the production with its associated environmental impact. Thus, this manuscript bets in the application of aqueous solutions of a cholinium-based ionic liquid (IL) containing glycinate as anion ([N1112OH][Gly]) for...
Article
Several molecular dynamics (MD) simulations have been performed in order to obtain structural information on ionic liquids (ILs) based on amino-acid anions. Six hydrophilic ILs containing cholinium or imidazolium cations combined with alaninate, glycinate or lysinate anions were modelled using the all-atom CL&P and OPLS-AA force fields. Both pure I...
Article
The definition of halophiles as "the coming stars of industrial biotechnology" in a recent review demands new research efforts for their efficient production at bioreactor scale. In this sense, the scarcity of information about halolipases production has furthered the research on the viability of Halomonas sp. LM1C culture in a mechanically stirred...
Article
Aqueous solutions of the non-ionic surfactants Triton X-100 and Triton X-102 have been salted out with the biocompatible ionic liquid cholinium dihydrogencitrate (N1112OHDHC). The phase boundary for {Triton X-100 or Triton X-102 + N1112OHDHC + H2O} was ascertained at T = (293.15, 303.15, 313.15 and 323.15) K and 0.1 MPa. The binodal data were model...
Article
This work is a first approach to demonstrate the viability of a quantitative biotechnological removal of choline chloride from aqueous effluents. Despite the much vaunted biocompatibility of this ammonium-based ionic liquid it was demonstrated (both by an ad-hoc OECD F test and flask assays) that different microorganisms able to degrade persistent...
Article
In this study, the viability of using biological induction as an alternative to the conventional chemical induction in lipase production by a novel halophilic microorganism, Halomonas sp. LM1C, has been demonstrated. Thus, a 9-times increase of lipase activity (3000 U/L) was recorded when Staphylococcus equorum sp. AMC7 was present in the medium, w...
Article
The extraction of phenolic acids from wheat straw (WS) by means of more environmentally friendly and competitive means is targeted in the present work. The ability of Tween 20 (Polyoxyethylene (20) sorbitan monolaurate) as phenolics extractant in aqueous solutions of a biocompatible ionic liquid (choline dihydrogencitrate) obtained after biomass hy...
Article
The potential of a commercially available imidazolium-based ionic liquid (C2C1imC1SO4) to selectively separate two model emerging contaminants, ethynylestradiol and clenbuterol, from aqueous solutions containing non-ionic surfactants (Triton X-100 and Triton X-102) was proved. After a first stage where the binodal curves and tie-lines of the system...
Chapter
Extremophiles are fascinating microorganisms that possess a recent research history. Since the late 1960s, when a strain of a thermophile was isolated from a hot spring, extremophiles have left academic laboratories and found their way into a variety of technological applications. These bizarre microorganisms are capable of surviving in extreme hab...
Chapter
This chapter sketches the main research trends concerning the functionalities of enzymes as food additives. In the past years full swing has been given to the role of enzymes to control aspects like texture, freshness, tenderness, flavor, and odor in a variety of food sectors like dairy, bread, meat, oleochemistry, or brewing. Additionally, the inc...
Article
The viability of the scaling-up of pectinases production by Aspergillus flavipes at 5 L-bioreactor scale has been demonstrated by keeping constant the power input, and a drastic increase in the endo- and exopectinolytic enzyme production was recorded (7- and 40-fold, respectively). The main process variables were modelled by means of logistic and G...
Article
Two imidazolium-based ionic liquids, 1-ethyl-3-methyl imidazolium methylsufate (C2C1imC1SO4) and 1-ethyl-3-methyl imidazolium ethylsulfate (C2C1imC2SO4), were tested as contenders for phase segregation in aqueous solutions of a non-ionic surfactant belonging to Tween family (Tween 20). Several empirical equations were used to correlate the binodal...
Article
The presence of emerging contaminants such as antibiotics is currently urging the search of new techniques to efficiently remove them from the environment. In this work, the exceptional ability of a cholinium-based ionic liquid to salt out aqueous solutions of non-ionic surfactants containing tetracycline and oxytetracycline has been demonstrated....
Article
Pseudomonas stutzeri strains are able to remediate different persistent contaminants, although they fail to efficiently treat industrial dyes. In this work, we have demonstrated that the acclimation to the presence of ionic liquids of a Pseudomonas stutzeri strain can be a solution to remove two synthetic dyes widely found in waste water effluents...
Article
Globally, tougher legislation in greenhouse gases emissions is prompting the hunt for more sustainable and competitive strategies to synthesize biodiesel. Although enzyme-catalyzed processes are considered as a more environmentally-friendly option, there are still challenges to be approached like the replacement of traditional solvents due to their...
Article
High-salinity biotopes in Turkey and Spain have been demonstrated to be a suitable source of lipolytic enzyme-producing halophilic microorganisms. Three strains turned out to grow at high NaCl concentration (greater than 15%) and their ability to synthesize lipolytic enzymes was demonstrated on tributyrin and olive oil-containing plates by the form...
Article
This study deals with the influence of different inorganic and organic salts made up with sodium, potassium and ammonium cations to induce phase segregation in aqueous solutions of C8C1imCl and C10C1imCl at T = 298.15 K. The experimental solubility values are described by means of four empirical equations and the suitability of the models was analy...
Article
A Pseudomonas stutzeri strain acclimated to the presence of neoteric contaminants has been proposed for simultaneously remediating an effluent polluted with Polycyclic Aromatic Hydrocarbons and a diazo dye. The pollutants chemical nature imposed a strict control of both the medium composition and the operating conditions. pH, temperature and agitat...
Article
The immiscibility windows of aqueous solutions containing the ionic liquid cholinium chloride (N1112OHCl) and the non-ionic surfactants Triton X-100 and Triton X-102 have been determined by the cloud point method at temperatures ranging from T = (298.15 to 333.15) K. The experimental values have been correlated by using two well-known equations. Th...
Article
Ionic liquids have been successfully proposed to modify membrane permeability in cultures of a model extremophilic bacterium Thermus thermophilus HB27, which makes up the first time that aqueous solutions of these molten salts are applied in downstream stages of this kind of microorganisms. The presence of 1g/L of C10MIMCl entails a great solubilis...
Article
Full-text available
A strategy based on the use of an inert ionic liquid (IL) has allowed the study of the electrolyte diffusion process in a polysiloxane-based coating applied on steel. The selection of 1-ethyl-3-methylimidazolium ethylsulfate, acting as nonaqueous medium, made it possible to evaluate the protective performance of polysiloxane coating under dry condi...
Article
One out of 10 microorganisms from extreme locations was adapted to the presence of common families of ionic liquids, which have lately emerged as “contaminants on the horizon”. A 10-fold higher tolerance was concluded for the ionic liquid-resistant strain. A biopolymer was secreted as an adaptation response.
Article
Full-text available
One of the challenges in the field of biocatalysis is the search for efficient reaction media avoiding enzyme deactivation. This work shows for the first time that water + hydrophilic ionic liquids mixtures enhance (up to 50%) the enzymatic activity of lipases, opening new opportunities for enzyme extraction and biocatalysis.
Article
Full-text available
In this work, the suitability of the ionic liquid 1-ethyl-3-methyl imidazolium ethylsulfate (C2C1imC2SO4) for improving the anticorrosive behaviour of zinc rich paints (ZRP) has been demonstrated. The electrochemical performance of the coating has been evaluated by means of electrochemical impedance spectroscopy. These data allowed validating the u...
Article
BACKGROUND Chitinases have been widely studied and can be produced by various groups of microorganisms, in particular, marine bacteria. The exploration of extremophilic marine microorganisms bears advantageous and unique biotechnological implications and the scaling up of the production process is a decisive stage to be researched. RESULTSA chitina...
Article
Full-text available
Using ionic liquids as co-solvents may improve reaction media in enzyme-based biotechnological processes. To establish new conditions, large libraries need to be screened for bio-compatibility and protein stabilisation. Using a lipase model, we herein describe a combination of methods leading to an expedited evaluation of 61 different solvent compo...
Article
Full-text available
The aqueous nature of aqueous biphasic systems has boosted their use in downstream stages in biotechnological processes. Since aqueous solutions of non-ionic surfactants are widely used for different metabolites' purification, we have demonstrated for the first time their segregation capacity in the presence of ionic liquids.
Article
An environmentally friendly heavy metals remediation process from polluted marine sediments is proposed. The efficiency of three organic and inorganic salts (ammonium acetate, ammonium nitrate, and sodium potassium tartrate) to salt out these pollutants was ascertained in sediment washing waters containing nonionic surfactants. The immiscibility re...
Article
Contamination of the environment with polycyclic aromatic hydrocarbons is one of the major problems facing the industrialized nations today. In this work, Pseudomonas stutzeri CET 930 was studied for the first time as bioremediation agent for the degradation of effluents containing phenanthrene, pyrene and benzanthracene, both individually and mixe...
Article
Full-text available
Phase segregation in aqueous biphasic systems (ABS) composed of four hydrophilic ionic liquids (ILs): 1-butyl-3-methylimidazolium methylsulfate and 1-ethyl-3-methylimidazolium methylsulfate (CnC1im C1SO4, n = 2 and 4), tributylmethyl phosphonium methylsulfate (P4441 C1SO4) and methylpyridinium methylsulfate (C1Py C1SO4) and two high charge density...
Article
Chlorpyrifos (CP) is one of the most commonly applied insecticides for control of pests and insects. The inappropriate use of this kind of chemicals has caused heavy contamination of many terrestrial and aquatic ecosystems thus representing a great environmental and health risk. The main purpose of this work is to investigate novel microbial agents...
Article
In this work, a novel remediation strategy consisting of a sequential biological and physical process is proposed to remove dyes from a textile polluted effluent. The decolorization ability of Anoxybacillus flavithermus in an aqueous effluent containing two representative textile finishing dyes (Reactive Black 5 and Acid Black 48, as di-azo and ant...
Article
Two endochitinases from Lactococcus lactis and Bacillus halodurans were expressed in E. coli, and the protein production process was performed at a bioreactor scale. The effect of engineering variables such as aeration and agitation on the performance of the biological process was determined; maximum levels of activity were achieved at an aeration...
Article
In this work, the experimental values of density, speed of sound, refractive index and dynamic viscosity have been obtained from T = (293.15 to 343.15) K for the three phosphonium-based ionic liquids: tributyl methyl phoshponium methylsulfate (P-4441 C1SO4), tributyl ethyl phosphonium diethylphosphate (P-4442 (C-2)(2)PO4) and tributyl octyl phospho...
Article
A new microbial consortium made up with two bacterial strains phylogenetically identified as Bacillus pumilus and Staphylococcus warneri was obtained from lab soils polluted with polycyclic aromatic hydrocarbons (PAHs) and heavy metals. Its PAH degradation potential has been demonstrated recently by culturing the consortium in the presence of phena...
Article
Recent alternatives to technological applications most commonly employed when the production of extremocompounds is approached at bioreactor scale, in batch, fed-batch, and continuous mode, are summarized. Each operational mode is ana-lyzed in the light of the more decisive variables affecting the performance of this kind of biological processes, n...
Article
a b s t r a c t The salting out potential of potassium-based inorganic salts was assessed in aqueous solutions of two non-ionic surfactants from the Tween family. New solubility data of the systems {surfactant (Tween 20/Tween 80) + inorganic salt (K 3 PO 4 /K 2 CO 3 /K 2 HPO 4 /K 2 S 2 O 3 /K 2 SO 3) + H 2 O} were experimentally ascer-tained at T =...
Article
a b s t r a c t The salting out potential of potassium-based inorganic salts was assessed in aqueous solutions of two non-ionic surfactants from the Tween family. New solubility data of the systems {surfactant (Tween 20/Tween 80) + inorganic salt (K 3 PO 4 /K 2 CO 3 /K 2 HPO 4 /K 2 S 2 O 3 /K 2 SO 3) + H 2 O} were experimentally ascer-tained at T =...
Article
Several hot springs in Galicia (North Western Spain) have been investigated as potential sources of lipolytic enzyme-producing thermophilic microorganisms. After isolating 12 esterase producing strains, 9 of them were assured to be true lipase producers, and consequently grown in submerged cultures, obtaining high extracellular activities by two of...
Article
In the present work, biodegradation of phenanthrene by a bacterial consortium (LB2), isolated from lab-polluted soils has been investigated. The 16S rRNA gene-based molecular analysis revealed that the bacterial consortium LB2 consisted of two strains showing a very high homology with Staphylococcus warneri and Bacillus pumilus. The optimization of...