Francisco Javier Burgos FlórezUniversidad del Norte (Colombia) | Uninorte · Department of Mechanical Engineering
Francisco Javier Burgos Flórez
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A current challenge regarding microfluidic paper-based analytical devices (µPAD) for blood plasma separation (BPS) and electrochemical immunodetection of protein biomarkers is how to achieve a µPAD that yields enough plasma to retain the biomarker for affinity biosensing in a functionalized electrode system. This paper describes the development of...
Point-of-Care (POC) testing for biomarker detection demands techniques that are easy to use, readily available, low-cost, and with rapid response times. This paper describes the development of a fully open-source, modular, wireless, battery-powered, smartphone-controlled, low-cost potentiostat capable of conducting electrochemical impedance spectro...
Neuronal damage secondary to traumatic brain injury (TBI) is a rapidly evolving condition, which requires therapeutic decisions based on the timely identification of clinical deterioration. Changes in S100B biomarker levels are associated with TBI severity and patient outcome. The S100B quantification is often difficult since standard immunoassays...
Background and Objective: To simulate infant skull trauma after low height falls when variable degrees of ossification of the sutures are present. Methods: A finite elements model of a four-week-old infant skull was developed for simulating low height impact from 30 cm and 50 cm falls. Two impacts were simulated: An occipito-parietal impact on the...
This work develops a methodology based on real chemical plant data collected from a Nitrogen-Phosphorus-Potassium fertilizer (NPK) cooling rotary drum. By blending thermodynamic variables given by global energy and mass balances with computational fluid dynamics-discrete element method (CFD-DEM) modeling and simulation, the methodology provides an...
lepharitis is a common chronic eye condition that causes eyelid inflammation, leading to inflamed, irritated, sticky and itchy eyelids and flaking of the skin. For its treatment, patients often need indefinite use of an eyelid cleaning solution which usually cost more than 20 USD per 80 ml bottle and lasts, on average, one month. For those patients...
Introduction The occurrence of adverse drug reactions is an important issue due to the lack of drug safety data in children. Objective To describe the Adverse Drug Reactions in inpatient children under 6 years of age in two general pediatrics wards located in Barranquilla, Colombia. Methods A prospective cohort study based on intensive pharmacovi...
The processes of flat bones growth, sutures formation and interdigitation in the human calvaria are controlled by a complex interaction between genetic, biochemical and environmental factors that regulate bone formation and resorption during prenatal development and infancy. Despite previous experimental evidence accounting for the role of the main...
My research is focused on the development of an electrochemical paper based biosensor that is capable of blood plasma separation and the following quantification of proteins released into the bloodstream when traumatic brain injury occurs. I am currently developing the first part of the device following the protocol designed by Noiphung et al (2013, https://www.ncbi.nlm.nih.gov/pubmed/23845479) which uses the wax printing method for creating hydrophilic channels where plasma is obtained from whole blood by means of capillary action. However, having done a literature review, Kersaudy et al. (2013, https://www.ncbi.nlm.nih.gov/pubmed/23824514) mentioned this: “While paper can be used conveniently to separate several microliters of blood such as finger-prick volumes, it rapidly clogs and is unsuitable for larger volumes, limiting its use to the analysis of highly concentrated analytes, such as glucose or abundant proteins". The problem lies in the fact that the proteins I want to detect have an average concentration in serum of 0.3pg/mL and with my paper microfluidic device I could obtain a maximum of 100 microliters of plasma in the detection zone. Since the electrochemical essay comprises an antigen-antibody binding, I am not sure whether I will be able to achieve a detection limit low enough to detect the average protein concentration of 0.3pg/mL. Hence, I would like to know if someone is currently working on a problem such as the one I am currently facing.
I appreciate in advance your help. All suggestion are welcome.