Francisco Borrego

Francisco Borrego
Biocruces Health Research Institute · Immunopathology

MD, PhD

About

128
Publications
15,639
Reads
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5,150
Citations
Introduction
Additional affiliations
March 2013 - present
Biocruces Health Research Institute, Spain, Barakaldo
Position
  • Ikerbasque Research Professor
October 2008 - February 2013
Center for Drug Evaluation and Research
Position
  • Senior Staff Fellow
July 2000 - October 2008
National Institutes of Health
Position
  • Researcher

Publications

Publications (128)
Article
Full-text available
Natural killer (NK) cells are lymphocytes with a key role in the defense against viral infections and tumor cells. Although NK cells are classified as innate lymphoid cells (ILCs), under certain circumstances they exhibit adaptive and memory-like features. The latter may be achieved, among others, by a brief stimulation with interleukin (IL)-12, IL...
Chapter
Natural killer (NK) cells are part of the innate immune system, the classic cytotoxic population of innate lymphoid cells (ILCs). They can directly kill virus-infected or tumor cells through different mechanisms without prior sensitization using their lytic functions in response to different signals (target cell ligands and/or inflammatory cytokine...
Chapter
Natural Killer (NK) cells are effector lymphocytes with the ability to generate an antitumor response. NK cells encompass a diverse group of subsets with different properties and have the capacity to kill cancer cells by different means. However, tumor cells have developed several mechanisms to evade NK cell-mediated killing. In this chapter, we su...
Article
Full-text available
Background: Infants with severe atopic dermatitis (AD) may be sensitized to foods that have not been introduced into their diet, posing a risk for developing an immediate hypersensitivity reaction on the first exposure to the food to which they are sensitized. The aim of this work was to perform an analysis of the sensitization profile in infants w...
Article
Full-text available
Autologous hematopoietic stem cell transplantation (autoHSCT) is a standard of care for transplant-eligible patients with multiple myeloma (MM). Among factors that influence outcome after autoHSCT, it has been suggested that the number of natural killer (NK) cells plays an important role. However, the impact that different NK cell subsets and their...
Article
Natural killer (NK) cells are lymphocytes able to exert potent antitumor and antiviral functions by different means. Besides their classification as innate lymphoid cells (ILCs), NK cells exhibit memory-like and memory responses after cytokine preactivation, viral infections and hapten exposure. Multiple NK cell-based immunotherapies have been deve...
Article
Emerging evidences show that innate immune cells can display changes in their functional programs after infection or vaccination, which lead to immunomodulation (increased or reduced responsiveness) upon secondary activation to the same stimuli or even to a different one. Innate cells acquire features of immunological memory, nowadays using the new...
Article
Full-text available
Natural killer (NK) cells are phenotypically and functionally diverse lymphocytes with the ability to recognize and kill malignant cells without prior sensitization, and therefore, they have a relevant role in tumor immunosurveillance. NK cells constitute the main lymphocyte subset in peripheral blood in the first week after hematopoietic stem cell...
Article
Full-text available
Natural Killer (NK) cells acquire memory-like properties following a brief stimulation with IL-12, IL-15 and IL-18. These IL-12/15/18-preactivated NK cells, also known as cytokine-induced memory-like (CIML) NK cells, have been revealed as a powerful tool in cancer immunotherapy due to their persistence in the host and their increased effector funct...
Article
Full-text available
COVID-19 manifests with a wide diversity of clinical phenotypes characterized by dysfunctional and exaggerated host immune responses. Many results have been described on the status of the immune system of patients infected with SARS-CoV-2, but there are still aspects that have not been fully characterized or understood. In this study, we have analy...
Preprint
Full-text available
COVID-19 manifests with a wide diversity of clinical phenotypes characterized by dysfunctional and exaggerated host immune responses. Many results have been described on the status of the immune system of patients infected with SARS-CoV-2, but there are still aspects that have not been fully characterized. In this study, we have analyzed a cohort o...
Article
Full-text available
Although scarce in the peripheral blood of healthy people, CD56neg NK cells are known to be expanded in some pathological conditions. However, studies on CD56neg NK cells had revealed contradictions, probably due to the lack of a specific NK cell surface marker that helps to identify this subset. This protocol details the step-by-step procedure for...
Article
Full-text available
Natural killer (NK) cells are lymphocytes with potent antitumor functions and, therefore, multiple NK cell-based cancer immunotherapies have been developed and are currently being tested. However, there is a necessity to find new means to improve these therapies, and immunometabolism represents an attractive target. NK cell effector functions are i...
Preprint
Full-text available
Natural Killer (NK) cells acquire memory-like properties following a brief stimulation with IL-12, IL-15 and IL-18. These IL-12/15/18-stimulated NK cells, also known as cytokine-induced memory-like (CIML) NK cells, have been revealed as a powerful tool in cancer immunotherapy due to their persistence in the host and their increased effector functio...
Article
Full-text available
Natural killer (NK) cells are usually identified by the absence of other lineage markers, due to the lack of cell surface specific receptors. CD56neg NK cells, classically identified as CD56negCD16+, are very scarce in the peripheral blood of healthy people but they expand in some pathological conditions. However, studies on CD56neg NK cells had re...
Article
Full-text available
Allergy is the host immune response against non-infectious substances called allergens. The prevalence of allergic diseases is increasing worldwide. However, while some drugs counteract the symptomatology caused by allergic reactions, no completely effective treatments for allergic diseases have been developed yet. In this sense, the ability of sur...
Article
Full-text available
CD300a receptor is found on different CD8+ T cell subsets and its expression has been associated to a more cytotoxic molecular signature. CD300a has an important role in some viral infections and its expression levels are known to be modulated by human immunodeficiency virus (HIV)−1 infection on several cell types. The main objective of this work w...
Article
Full-text available
Human CD300a is known to promote the infection by Dengue and other enveloped viruses and is overexpressed on CD4+ T cells from HIV-1 infected patients. We found that infected CD4+RA- T cells from untreated HIV-1 infected patients were mostly CD300a+. Furthermore, CD300a expressing CD4+RA- T cells from healthy donors were significantly more infected...
Preprint
Full-text available
Natural killer (NK) cells are usually identified by the absence of other lineage markers, due to the lack of a cell surface specific marker. CD56neg NK cells, classically identified as CD56negCD16+ are known to be expanded in some pathological conditions. However, studies on CD56neg NK cells had revealed different results regarding the phenotype an...
Article
Full-text available
Natural killer (NK) cells are cytotoxic lymphocytes that are able to kill tumor cells without prior sensitization. It has been shown that NK cells play a pivotal role in a variety of cancers, highlighting their relevance in tumor immunosurveillance. NK cell infiltration has been reported in renal cell carcinoma (RCC), the most frequent kidney cance...
Article
Full-text available
Natural Killer (NK) cells are characterized by their potential to kill tumor cells by different means without previous sensitization and have, therefore, become a valuable tool in cancer immunotherapy. However, their efficacy against solid tumors is still poor and further studies are required to improve it. One of the major restrictions for NK cell...
Poster
Full-text available
It is well known that activation with interleukin (IL)-15 enhance the survival of NK cells (NKs), cytotoxic activity against target cells and the production of cytokines (i.e. interferon-gamma). Nano-particles (NPs) coated with activating molecules, such as IL-15, could be an effective new approach to generate potent immune responses against cancer...
Article
Full-text available
The CD300 molecules constitute an evolutionarily significant family of receptors that are expressed on myeloid and lymphoid cells, but also on other cell types, such as tuft cells. Many of the CD300 receptors have been shown to recognize lipids, e.g. phosphatidylserine and phosphatidylethanolamine. Over the past couple of years, accumulating eviden...
Chapter
Lymphocytes proliferate in response to several stimuli. In many situations, a rapid lymphocyte expansion, or the identification of a slow dividing cell subpopulation may be of great interest. Thus, it is necessary to perform reliable assays to study and compare lymphocyte subsets proliferation. For this purpose, carboxifluorescein diacetate succini...
Data
Analysis of IL22RA2 and cytokine gene expression by qPCR and surface expression markers by flow cytometry in CD16−/CD14+ or CD16+ monocytes and their corresponding derived immature and mature dendritic cells.
Data
Supplementary Figure 1 (Strategy for monocyte subpopulation sorting); Supplementary Figure 2 (Flow cytometric analysis of moDCs); Supplementary Figure 3 (IL-22BPi2 detection with different antibodies); Supplementary Figure 4 (Cell fractionation); Supplementary Figure 5 (Protein partners of IL-22BPi1 and IL-22BPi2, WB-validation); Supplementary Figu...
Article
Full-text available
The human IL22RA2 gene co-produces three protein isoforms in dendritic cells [IL-22 binding protein isoform-1 (IL-22BPi1), IL-22BPi2, and IL-22BPi3]. Two of these, IL-22BPi2 and IL-22BPi3, are capable of neutralizing the biological activity of IL-22. The function of IL-22BPi1, which differs from IL-22BPi2 through an in-frame 32-amino acid insertion...
Poster
Full-text available
It is well known that activation of NK cells with interleukin (IL)-15 enhance their survival, cytotoxic activity against target cells and the production of cytokines (i.e. interferon-gamma). NPs coated with activating molecules, such as IL-15, could be an effective new approach to generate potent immune responses against cancer, for example by incr...
Article
Full-text available
The ability of the CD300a inhibitory receptor to modulate immune cell functions and its involvement in the pathogenesis of many diseases has aroused a great interest in this molecule. Within human CD4+ T lymphocytes from healthy donors, the inhibitory receptor CD300a is differentially expressed among different T helper subsets. However, there are n...
Article
Full-text available
Background: Basophils express high-affinity IgE receptors (FcεRI), which play an essential role in allergic diseases. To design promising immunomodulatory therapies, it is important to characterize new cell surface receptors that modulate immunoglobulin (Ig)E-mediated basophil activation threshold. Objectives: To analyze the expression of CD300...
Article
Full-text available
A brief in vitro stimulation of natural killer (NK) cells with interleukin (IL)-12, IL-15, and IL-18 endow them a memory-like behavior, characterized by higher effector responses when they are restimulated after a resting period of time. These preactivated NK cells, also known as cytokine-induced memory-like (CIML) NK cells, have several properties...
Poster
Full-text available
Some of the most promising new therapies to treat cancer are based on harnessing the immune system to target tumours. Among these novel therapies is the application of dendritic cells (DCs) and natural killer (NK) cells that are ex vivo selected and educated to enhance the innate and adaptive immune system response against the tumour cells [1]. Thi...
Article
Full-text available
A modified vaccinia Ankara-based HIV-1 vaccine clade B (MVA-B) has been tested for safety and immunogenicity in low-risk human immunodeficiency virus (HIV)-uninfected individuals and as a therapeutic vaccine in HIV-1-infected individuals on combined antiretroviral therapy (cART). As a therapeutic vaccine, MVA-B was safe and broadly immunogenic; how...
Data
Total number of CD56+ cells at different time points during the in vitro differentiation protocol with three different culture conditions: OP9 and M2-10B4 cells coculture (N:4) and feeder-free (N:2).
Data
Dot-plots of the expression of CD3 at different stages of natural killer cells during the differentiation protocol.
Data
Number of fresh umbilical cord blood (UCB) units, volume in milliliters, number of mononuclear cells per UCB, and CD34+ cells remaining after purification for each experiment. UCB were fresh, being not longer than 30 h after the extraction.
Data
(A) Purity of CD34+ cell population from umbilical cord blood (UCB) obtained by immunomagnetic isolation. (B) Remaining percentage of CD56+ cells after purification of CD34+ cells from UCB.
Data
Key markers expressed at different stages through natural killer cell differentiation/maturation in vivo and in vitro.
Article
Full-text available
Background: Sepsis is a life-threatening illness with a challenging diagnosis. Current serum biomarkers are not sensitive enough for diagnosis. With the aim of finding proteins associated with sepsis, serum protein profile was compared between patients and healthy donors and serum classical inflammatory proteins were analyzed in both groups. Meth...
Article
Full-text available
Adoptive natural killer (NK) cell therapy relies on the acquisition of large numbers of mature and functional NK cells. An option for future immunotherapy treatments is to use large amounts of NK cells derived and differentiated from umbilical cord blood (UCB) CD34+ hematopoietic stem cells (HSCs), mainly because UCB is one of the most accessible H...
Article
Full-text available
Transforming growth factor-beta (TGFβ) in the tumor microenvironment diminishes natural killer (NK) cell-mediated anti-disialoganglioside (anti-GD2) monoclonal antibody (mAb) elimination of neuroblastoma cells. Consequently, blockade of the TGFβ signaling with galunisertib, in combination with anti-GD2 mAb dinutuximab plus adoptively transferred NK...
Article
Full-text available
Neonates are more susceptible to infections than adults. This susceptibility is thought to reflect neonates’ qualitative and quantitative defects in the adaptive and innate immune responses. Differential expression of cell surface receptors may result in altered thresholds of neonatal immune cell activation. We determined whether the expression and...
Article
Full-text available
Fc receptor-like (FCRL) 5 is a novel IgG binding protein expressed on B cells, with the capacity to regulate Ag receptor signaling. We assessed FCRL5 expression on circulating B cells from healthy donors and found that FCRL5(+)cells are most enriched among atypical CD21(-/lo)/CD27(-)tissue-like memory (TLM) B cells, which are abnormally expanded in...
Article
Full-text available
Paired receptors on NK cells recognize similar ligands with varied strength of binding ability and perform different functions. The CD300 molecules are emerging as novel immune regulators in health and disease due to their interaction with their lipid-nature ligands. Particularly, the paired receptors CD300c and CD300a have been shown to elicit act...
Article
Full-text available
The CD300a inhibitory receptor belongs to the CD300 family of cell surface molecules that regulate a diverse array of immune cell processes. The inhibitory signal of CD300a depends on the phosphorylation of tyrosine residues embedded in ITIMs of the cytoplasmic tail. CD300a is broadly expressed on myeloid and lymphoid cells, and its expression is d...
Article
Full-text available
The highly conserved matrix protein 2 (M2) is a good candidate for the development of a broadly protective influenza vaccine that induces long-lasting immunity. In animal models, natural killer (NK) cells have been proposed to play an important role in the protection provided by M2-based vaccines through a mechanism of antibody-dependent cell-media...
Article
Full-text available
Natural killer (NK) cells play an essential role in the fight against tumor development. Over the last years, the progress made in the NK-cell biology field and in deciphering how NK-cell function is regulated, is driving efforts to utilize NK-cell-based immunotherapy as a promising approach for the treatment of malignant diseases. Therapies involv...
Article
Full-text available
Besides their essential role in hemostasis and thrombosis, platelets are involved in the onset of cancer metastasis by interacting with tumor cells. Platelets release secretory factors that promote tumor growth, angiogenesis, and metastasis. Furthermore, the formation of platelet–tumor cell aggregates in the bloodstream provides cancer cells with a...