Francieli Colussi

Francieli Colussi
University of Minho · Departamento de Engenharia Biológica (DEB)

PhD

About

18
Publications
4,175
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352
Citations
Introduction
Francieli Colussi currently works at the Departamento de Bioquímica e Biologia Molecular (DBBM), Universidade Federal de Goiás. Their current project is 'Genes alvos para o melhoramento genético de fungos e plantas'.
Additional affiliations
August 2014 - August 2016
Roskilde University
Position
  • Researcher
October 2012 - July 2013
Universidade Federal do ABC (UFABC)
Position
  • Researcher
March 2008 - October 2012
University of São Paulo
Position
  • PhD Student

Publications

Publications (18)
Article
Full-text available
The β-glucosidases are enzymes essential for several industrial applications, especially in the field of plant structural polysaccharides conversion into bioenergy and bioproducts. In a recent study, we have provided a biochemical characterization of two hyperthermostable β-glucosidases from Thermotoga petrophila belonging to the families GH1 (TpBG...
Article
Full-text available
Cellobiohydrolases (CBHs) break down cellulose sequentially by sliding along the crystal surface with a single cellulose strand threaded through the catalytic tunnel of the enzyme. This so-called processive mechanism relies on a complex pattern of enzyme-substrate interactions, which need to be addressed in molecular descriptions of processivity an...
Article
Adsorption of cellulases on the cellulose surface is an integral part of the catalytic mechanism, and a detailed description of the adsorption process is therefore required for a fundamental understanding of this industrially important class of enzymes. However, the mode of adsorption has proven intricate, and several key questions remain open. Per...
Article
Full-text available
Endo-β-1,4-mannanase from Thermotoga petrophila (TpMan) is a hyperthermostable enzyme that catalyzes the hydrolysis of β-1,4-mannoside linkages in various mannan-containing polysaccharides. A recent study reported that TpMan is composed of a GH5 catalytic domain joined by a linker to a carbohydrate-binding domain. However, at this moment, there is...
Article
Aiming to contribute towards the characterization of new, biotechnologically relevant cellulolytic enzymes, we report here the first crystal structure of the catalytic core domain of Cel7A (CBHI) from the filamentous fungus Trichoderma harzianum IOC 3844. Our structural studies and molecular dynamics simulations show that the flexibility of Tyr260,...
Chapter
In the last decade, there was a great effort to promote lignocellulosic biomass (LCB) as a raw material for obtaining new added value compounds. Agricultural and industrial processing activities generate a large volume of by-products, most of which are still little explored, representing a significant risk to the environment. LCB is an abundant and...
Article
One full-length β-xylosidase gene (hxylA) was identified from the Humicola grisea var. thermoidea genome and the cDNA was successfully expressed by Pichia pastoris SMD1168. An optimization of enzyme production was carried out, and methanol was found to be the most important parameter. The purified enzyme was characterized and showed the optimal con...
Article
Full-text available
One full-length β-xylosidase gene (hxylA) was identified from the Humicola grisea var. thermoidea genome and the cDNA was successfully expressed by Pichia pastoris SMD1168. An optimization of enzyme production was carried out, and methanol was found to be the most important parameter. The purified enzyme was characterized and showed the optimal con...
Article
Full-text available
Endo-β-1, 4-mannanase from Thermotoga petrophila (TpMan) is a modular hyperthermo-stable enzyme involved in the degradation of mannan-containing polysaccharides. The degradation of these polysaccharides represents a key step for several industrial applications. Here, as part of a continuing investigation of TpMan, the region corresponding to the GH...
Article
Full-text available
Due to its elevated cellulolytic activity, the filamentous fungus Trichoderma harzianum (T. harzianum) has considerable potential in biomass hydrolysis application. Cellulases from Trichoderma reesei have been widely used in studies of cellulose breakdown. However, cellulases from T. harzianum are less-studied enzymes that have not been characteriz...
Article
Full-text available
The FnIII3-FnIII4 tandem has a radius of gyration (Rg), calculated from the SAXS data, of 21 Å, which is significant smaller than the Rg of the FnIII1-FnIII2 (~29 Å) indicating that the second pair of FnIII domains adopts a more compact structure than the first pair of FnIII domains. We have modelled the low resolution structure of the FnIII3-FnIII...
Article
Full-text available
Because of its elevated cellulolytic activity, the filamentous fungus Trichoderma harzianum has a considerable potential in biomass hydrolysis applications. Trichoderma harzianum cellobiohydrolase I (ThCBHI), an exoglucanase, is an important enzyme in the process of cellulose degradation. Here, we report an easy single-step ion-exchange chromatogra...
Article
Full-text available
The filamentous fungus Trichoderma harzianum has a considerable cellulolytic activity that is mediated by a complex of enzymes which are essential for the hydrolysis of microcrystalline cellulose. These enzymes were produced by the induction of T. harzianum with microcrystalline cellulose (Avicel) under submerged fermentation in a bioreactor. The c...
Article
Full-text available
This work aimed to study the in vitro regeneration of the weed Euphorbia heterophylla from hypocotyls, cotyledon and root explants grown under different concentrations of the hormone 2iP, combined or not with auxin added to MS/2 medium. The parameters analyzed were the number of explants with shoot, number of shoot for explants, number of rooted an...
Article
This work aimed to study the in vitro regeneration of the weed Euphorbia heterophylla from hypocotyls, cotyledon and root explants grown under different concentrations of the hormone 2iP, combined or not with auxin added to MS/2 medium. The parameters analyzed were the number of explants with shoot, number of shoot for explants, number of rooted an...
Article
Full-text available
The passion fruit (Passiflora edulis) is originary from Tropical America and has been growing largely in Brazil, being a crop very rich in vitamin C, calcium and phosphorus. Peels and seeds of Passion fruit that come from the cut and extraction process of fruit in order to extract juice are still throw out in large amounts. As such amounts depict l...
Article
Full-text available
O maracujá (Passiflora edulis) é originário da América Tropical, muito cultivado no Brasil, rico em vitamina C, cálcio e fósforo. Cascas e sementes de maracujá, provenientes do processo de corte e extração da fruta para obtenção do suco, são ainda, atualmente, em grande parte descartadas. Como este descarte representa inúmeras toneladas, agregar va...
Article
Full-text available
This work aimed to study the in vitro regeneration of the weed Euphorbia heterophylla from hypocotyls, cotyledon and root explants grown under different concentrations of the hormone 2iP, combined or not with auxin added to MS/2 medium. The parameters analyzed were the number of explants with shoot, number of shoot for explants, number of rooted an...

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Projects

Projects (2)
Archived project
Project
Genes de remodelamento de parede celular vegetal