francesco valle

francesco valle
Italian National Research Council | CNR · Institute of Nanostructured Materials ISMN

PhD

About

100
Publications
13,102
Reads
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2,241
Citations
Introduction
I have been in the selection commette of the International School for Advanced Studies in Trieste in 2006, charged of the selection of the PhD candidates
Additional affiliations
January 2005 - January 2008
University of Bologna
Position
  • PostDoc Position
Description
  • single molecule force spectroscopy of intrinsically disordered proteins
January 2004 - January 2005
École Polytechnique Fédérale de Lausanne
Position
  • PostDoc Position
Description
  • topological properties of DNA knots
Position
  • Research Assistant
Education
November 1999 - November 2003
University of Lausanne
Field of study
  • Physics
September 1992 - May 1999
Sapienza University of Rome
Field of study
  • Physics

Publications

Publications (100)
Article
Laser Assisted Bioprinting (LAB) is recognized to be an enabling and versatile microfabrication technology for regenerative medicine and artificial tissue engineering. Current bioprinting concentrates on a layer-by-layer approach to print cells in consecutive stacks or nets, to recreate specialized tissue functions with a top-down approach. This sy...
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Fullerenes are candidates for theranostic applications because of their high photodynamic activity and intrinsic multimodal imaging contrast. However, fullerenes suffer from low solubility in aqueous media, poor biocompatibility, cell toxicity, and a tendency to aggregate. C70@lysozyme is introduced herein as a novel bioconjugate that is harmless t...
Article
A general single‐step approach is introduced for the green fabrication of hybrid biosensors from water dispersion. The resulting device integrates the semiconducting properties of a carbon nanotube (CNT) and the functionality of a protein. In the initial aqueous phase, the protein (viz., lysozyme [LZ]) disperses the (6,5)CNT. Drop‐casting of the di...
Article
It is well known that amphiphilic cationic β-cyclodextrins (amβCDs) form nanovesicles able to release their cargo in aqueous solution upon applying different stimuli. In addition they can be selectively positioned onto substrates by unconventional soft lithography. This makes them a powerful tool for designing environments where different cues can...
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Bionic composites are an emerging class of materials produced exploiting living organisms as reactors to include synthetic functional materials in their native and highly performing structures. In this work, single wall carboxylated carbon nanotubes (SWCNT-COOH) were incorporated within the roots of living plants of Arabidopsis thaliana. This bioge...
Article
Keratin (Ker) protein regenerated from readily available and low-cost wastes (e.g. raw wool, feathers and textile by-products) is a promising natural polymer for tissue engineering and drug delivery. Nevertheless, while showing good bioactivity and great drug carrier properties, keratin lacks of suitable mechanical characteristics. To overcome this...
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Atomic force microscopy (AFM) besides X‐ray crystallography and electron microscopy is one of the most attractive methods to study bio/nonbio complexes. Information on how biomacromolecules interact with nanomaterials under different environmental conditions has important implications for the practice of nanomedicine and concerning the safety of na...
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The modification of amyloid fibrils cytotoxicity through exogenous nanomaterials is crucial to understand the processes controlling the role of protein aggregation in the related diseases. The influence of nanoparticles on amyloid stability yields great interest due to the small size and high surface area-to-volume ratio of nanoparticles. Various p...
Article
In article number 1900264, Annalisa Convertino, Valentina Benfenati, and co‐workers show that a forest of randomly oriented gold coated‐silicon nanowires (Au/SiNWs) induces the differentiation of astrocytes showing complex structural and functional properties in vivo. Tight junctions between the Au/SiNWs‐device and astrocytes processes enables extr...
Article
The preferred spatial orientation of single-wall carbon nanotubes (SWCNTs) in their interaction with enzymes determines their behavior either as nano-supports or as inhibitors α -chymotrypsin (α-CT) is considered a serine protease model for studying nanomaterial/proteases interactions. The interaction of α-CT with pristine single-wall carbon nanotu...
Article
The correct human brain function is dependent on the activity of non‐neuronal cells called astrocytes. The bioelectrical properties of astrocytes in vitro do not closely resemble those displayed in vivo and the former are incapable of generating action potential; thus, reliable approaches in vitro for noninvasive electrophysiological recording of a...
Article
Full-text available
Supramolecular hydrogels, obtained from small organic molecules, may be advantageous over polymeric ones for several applications, because these materials have some peculiar properties that differentiate them from the traditional polymeric hydrogels, such as elasticity, thixotropy, self-healing propensity, and biocompatibility. We report here the p...
Article
The controlled release of cell differentiating agents is crucial in many aspects of regenerative medicine. Here we propose the use of hybrid calcite single crystals as micro-carriers for the controlled and localized release of retinoic acid which is entrapped within the crystalline lattice. The release of retinoic acid occurs only in the proximity...
Article
Current technologies to monitor formation and disruption in in vitro cell cultures are based either on optical techniques or on electrical impedance/resistance measurement, which often rely on cumbersome and time‐consuming measurements and data analyses. In this paper, poly(3,4‐ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS)‐based organic...
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Full-text available
The formation of amyloid fibrils from soluble proteins is a common form of self-assembly phenomenon that has fundamental connections with biological functions and human diseases. Lysozyme was converted from its soluble native state into highly organized amyloid fibrils. Ultrasonic treatment was used to break amyloid fibrils to fibrillar fragments–s...
Article
In this work, nano-hybrid electrospun non-wovens made of wool keratin combined with diclofenac loaded hydrotalcites (HTD) were prepared and characterized as potential drug delivery systems and scaffolds for fibroblast cell growth. Nano-hybrid electrospun non-wovens showed a good adaptability to the wet skin, effortlessly conforming to the three-dim...
Article
Carbon nanotubes, CNTs, and proteins could not be more chemically and physically distant. CNTs are hardly soluble and strongly hydrophobic. They are smooth wires, mechanically strong, with electronic properties that make them unique. Many proteins are water soluble and hydrophilic. They are highly corrugated and elastic. They can recognize a target...
Article
The lack of solubility in water and the formation of aggregates hamper many opportunities of technological ex-ploitation of C60. Here different peptides were designed and synthesized with the aim of dispersing monomo-lecularly C60 in water. Phenylalanines were used as recognizing moieties, able to interact with C60 through π−π stacking, while a var...
Article
Hybrid systems have great potential for a wide range of applications in chemistry, physics and materials science. Conjugation of a biosystem to a molecular material can tune the properties of the components or make new ones arise. As a workhorse, here we take C60@lysozyme hybrid. We show that lysozyme recognizes and disperses fullerene in water. AF...
Preprint
The high hydrophobicity of fullerenes and the resulting formation of aggregates in aqueous solutions hamper the possibility of their exploitation in many technological applications. Noncovalent bioconjugation of fullerenes with proteins is an emerging approach for their dispersion in aqueous media. Contrary to covalent functionalization, bioconjuga...
Article
Full-text available
The high hydrophobicity of fullerenes and the resulting formation of aggregates in aqueous solutions hamper the possibility of their exploitation in many technological applications. Noncovalent bioconjugation of fullerenes with proteins is an emerging approach for their dispersion in aqueous media. Contrary to covalent functionalization, bioconjuga...
Article
The paper introduces data‐matrix technology in life science as an efficient method for cell culture monitoring. The proposed method, named Data‐Matrix method (DM method) “learns from errors” taking advantage of the robust know‐how and the practical tools, developed for error handling in digital data storage and satellite transmission technologies....
Preprint
The high hydrophobicity of fullerenes and the resulting formation of aggregates in aqueous solutions hamper the possibility of their exploitation in many technological applications. Noncovalent bioconjugation of fullerenes with proteins is an emerging approach for their dispersion in aqueous media. Contrary to covalent functionalization, bioconjuga...
Article
A data-matrix (DM)-technology approach in cell biology is implemented as an efficient method for the multiparameter monitoring of cell cultures. The proposed method takes advantage of the know-how developed for fault tolerance in digital information technology by measuring the amount of errors induced by intervening cells upon checking a DM code pl...
Article
The mussel byssus is a biorenewable, protein-based material produced by marine mussels, which has attracted the interest of material scientists due to its remarkable mechanical and self-healing properties. Large quantities of byssus waste material from mussel mariculture are produced every year, which have great potential as a raw starting material...
Article
Peptide aggregation into oligomers and fibrillar architectures is a hallmark of severe neurodegenerative pathologies, diabetes mellitus or systemic amyloidoses. The polymorphism of amyloid forms and their distribution are both effectors that potentially modulate the disease, thus it is important to understand the molecular basis of protein amyloid...
Article
Thienopyrrole-dione (TI) end capped materials have recently emerged as polymorphic molecular semiconductors suitable as active layers of ambipolar light emitting transistors, photovoltaic cells and time temperature integrator devices. Here, we report the synthesis of a new derivative, namely 2,2'-([2,2'-Bithiophene]-5,5'-diyl)bis(5-octyl-4-phenyl-4...
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Full-text available
While the widespread emergence of nanoscience and nanotechnology can be dated back to the early eighties, the last decade has witnessed a true coming of age of this research field, with novel nanomaterials constantly finding their way into marketed products. The performance of nanomaterials being dominated by their nanoscale morphology, their quant...
Article
The genome of Arabidopsis thaliana encodes three glucan, water dikinases. Glucan, water dikinase 1 (GWD1; EC 2.7.9.4) and phosphoglucan, water dikinase (PWD; EC 2.7.9.5) are chloroplastic enzymes, while glucan, water dikinase 2 (GWD2) is cytosolic. Both GWDs and PWD catalyze the addition of phosphate groups to amylopectin chains at the surface of s...
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In living cells, DNA is highly confined in space with the help of condensing agents, DNA binding proteins and high levels of supercoiling. Due to challenges associated with experimentally studying DNA under confinement, little is known about the impact of spatial confinement on the local structure of the DNA. Here, we have used well characterized s...
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The exploitation of nanomaterials with antimicrobial properties has attracted an ever-growing interest in the recent years. Carbon-based materials, such as graphene and graphene family materials (GFMs), have gained most of the attention for application in many biomedical fields. Here, we describe the antimicrobial activity of graphene-like (GL) lay...
Article
The toxic oxyanion tellurite (TeO3²⁻) is acquired by cells of Rhodobacter capsulatus grown anaerobically in the light, via acetate permease ActP2 and then reduced to Te⁰ in the cytoplasm as needle-like black precipitates. Interestingly, photosynthetic cultures of R. capsulatus can also generate Te⁰ nanoprecipitates (TeNPs) outside the cells upon ad...
Article
Mapping the morphological and nano-mechanical properties of cellulose fibers within paper sheets or textile products at the nano-scale level by using atomic force microscopy is a challenging task due to the huge surface level variation of these materials. However this task is fundamental for applications in forensic or cultural heritage sciences an...
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Potassium channels and aquaporins expressed by astrocytes are key players in the maintenance of cerebral homeostasis and in brain pathophysiologies. One major challenge in the study of astrocyte membrane channels in vitro, is that their expression pattern does not resemble the one observed in vivo. Nanostructured interfaces represent a significant...
Article
The toxic oxyanion tellurite (TeO32−) is acquired by cells of Rhodobacter capsulatus grown anaerobically in the light, via acetate permease ActP2 and then reduced to Te° in the cytoplasm as needle-like black precipitates. Interestingly, photosynthetic cultures of R. capsulatus can also generate Te° nanoprecipitates (TeNPs) outside the cells upon ad...
Article
We report on the fabrication of self-protective self-assembled monolayers constituted by a highly fluorescent component and a linear alkyl chain. We demonstrate the formation of interpenetrated molecular network in which the alkyl component acts as an ultra-thin protective layer able to effectively shielding the functional component from several ty...
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Full-text available
Calcium phosphate (CaP) coatings are commonly employed to improve the bioactivity of bone and dental metal implants, due to the chemical–physical similarity with the mineral phase of bone, envisaged to enhance the integration with the surrounding bone tissue. However, growing concerns about the use of commercial thick CaP coatings, mainly related t...
Article
The interactions of a biodegradable scaffold with cells or living tissues depend on the time-evolution of the nanoscale properties of the scaffold. We present an in situ quantitative study on the early-stage swelling and degradation of poly(lactic-co-glycolic acid) (PLGA). A novel metrology scheme based on force microscopy measurements of the patte...
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Full-text available
The squid pen (gladius) from the Loligo vulgaris was used for preparation of β-chitin materials characterized by different chemical, micro-and nano-structural properties that preserved, almost completely the macrostructural and the mechanical ones. The β-chitin materials obtained by alkaline treatment showed porosity, wettability and swelling that...
Article
Chinotto (Citrus×myrtifolia) is a uncommon fruit belonging to the Citrus genus, mainly cultivated in small areas of the Italian territory, where the main use concerns the eponymous drink, marketed with the name of "Chinotto". The lack of information about this fruit highlights the usefulness of nutraceutical compound characterization, as well as th...
Article
The design of β-cyclodextrin/multiwalled carbon nanotubes hybrid (β-CD-MWCNT) as nanoplatform for the entrapment and delivery of guanine based drugs is described here. The functionalized carbon nanomaterials have been characterized by XPS spectroscopy, electron microscopy (FEG-SEM and TEM), AFM, TGA, and FT-IR to achieve insights on structure, morp...
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Conspectus This Account aims to describe our experience in the use of patterning techniques for addressing the self-organization processes of materials into spatially confined regions on technologically relevant surfaces. Functional properties of materials depend on their chemical structure, their assembly, and spatial distribution at the solid sta...
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Anisotropic orientation and accurate positioning of neural cells is achieved by patterning stripes of the extracellular matrix protein laminin on the surface of polystyrene tissue culture dishes by micromoulding in capillaries (MIMICs). Laminin concentration decreases from the entrance of the channels in contact with the reservoir towards the end....
Article
This study is an investigation of the effect of biocompatible glutathione-covered gold nanoparticles (AuSG_7) with an average size of 3 nm on the amyloid fibrils of hen egg-white lysozyme. The anti-amyloid activity of AuSG_7 nanoparticles on this protein was monitored with thioflavin T assay, atomic force microscopy and transmission electron micros...
Article
This protocol describes how to perform lithographically controlled wetting (LCW). LCW enables large-area patterning of microstructures and nanostructures of soluble materials, either organic or inorganic, including biological compounds in buffer solutions or compounds for cell guidance. LCW exploits the capillary forces of menisci established under...
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This protocol provides the instructions for designing and fabricating stamping tools with features ranging from nanometer to micrometer scale, including the fabrication using commercial tools such as compact disks or digital video disks. In particular the reported procedures are oriented towards the tools fabrication for lithographically controlled...
Article
A novel application of replica molding to a forensic problem, viz. the accurate reproduction of the case head of gun and rifle cartridges, prior and after been shot, is presented. The fabrication of an arbitrary number of identical copies of the region hit by the firing pin and by the breech face is described. The replicas can be (i) handled withou...
Article
Titanium dioxide (TiO2) is a biocompatible material with important applications in the field of regenerative medicine. Here we show that a multi-scale hierarchical architecture of TiO2, realized with sub-micrometer polystyrene beads as templating agent patterned by micromolding in capillaries (MIMICs), is a viable functional tool for the systematic...
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Dewetting is a spontaneous phenomenon where a thin film on a surface ruptures into an ensemble of separated objects, like droplets, stripes, and pillars. Spatial correlations with characteristic distance and object size emerge spontaneously across the whole dewetted area, leading to regular motifs with long-range order. Characteristic length scales...
Article
Patterning cell-adhesive molecules on material surfaces provides a powerful tool for controlling and guiding cell locomotion and migration. Here we report fast, reliable, easy to implement methods to fabricate large patterns of proteins on synthetic substrates to control the direction and speed of cells. Two common materials exhibiting very differe...
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We propose an integrated top-down and bottom-up approach to single-step nanofabrication of complex nanostructures made of different materials. The process, termed lithographically controlled etching (LCE), starts with a drop of an etching solution cast on the surface to be patterned. By placing a polymeric mold on the substrate, the stamp protrusio...
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Chromosomal dissection provides a direct advance for isolating DNA from cytogenetically recognizable region to generate genetic probes for fluorescence in situ hybridization, a technique that became very common in cyto and molecular genetics research and diagnostics. Several reports describing microdissection methods (glass needle or a laser beam)...
Article
Amyloid-related diseases (such as Alzheimer's disease or diabetes type II) are associated with self-assembly of protein into amyloid aggregates. Spectroscopic and atomic force microscopy were used to determine the ability of acridines to affect amyloid aggregation of lysozyme. We have studied the effect of acridine derivatives on the amyloid aggreg...
Article
Extensive evidence suggests that the self-assembly of amyloid-beta peptide (Aβ) is a nucleation-dependent process that involves the formation of several oligomeric intermediates. Despite neuronal toxicity being recently related to Aβ soluble oligomers, results from aggregation studies are often controversial, mainly because of the low reproducibili...
Article
Micro- and nano-patterning of cell adhesion proteins is demonstrated to direct the growth of neural cells, viz. human neuroblastoma SHSY5Y, at precise positions on a strongly antifouling substrate of technolological interest. We adopt a soft-lithographic approach with oxygen plasma modified PDMS stamps to pattern human laminin on Teflon-AF films. T...