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Fouad Razzaq Al-Burki

Fouad Razzaq Al-Burki
Al-Muthanna university · Field crops science

Assist. Prof. Dr.- PhD by SupAgro-INRA France, Prof. In Al-Muthanna unv.

About

54
Publications
6,744
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Introduction
Assistant Professor at Al-Muthanna University, Iraq. Fellow of the Royal Society of Biology (London), member of the French Society of Biologists (Paris) and member of the Middle East Society of Molecular Biology (Dubai). My specialist in molecular genetics and plants breeding (Cereals), for example my work in investigates the role of transporters and ion channels in the plant and its adaptation to abiotic stresses: K+ deficiency, salinity and drought stresses. The plants studied are mainly cereals (rice , wheat, corn, barley ), for genetic improvement of cereal crops. Targeted transport systems mainly belong to the family of K+ and / or Na+ HKT transporters, and families of K+ transporters and another lines in plant breeding by genetic engineering.
Education
September 2013 - November 2017
French National Institute for Agriculture, Food, and Environment (INRAE)
Field of study
  • Molecular biology and plant genetics improvement

Publications

Publications (54)
Article
Full-text available
A field experiment was conducted at the rice research station in Al-Mishkhab, Najaf Governorate, Iraq during the 2019, to study the vegetative growth and yield traits of 12 genetically modified of rice genotypes (FR1, FR2, FR3, FR4, FR14, FR15, FR16, FR17, FR18, FR21, FR26, and FR27). The study included the traits of plant height, spike length, the...
Article
Full-text available
This study was conducted with the target of determine the role of OsHKT4 and OsHKT6 genes in rice plants under salt stress and observe its gene expression by GUS technology, as well as studying the Na ⁺ and K ⁺ accumulation in different tissues. The results obtained show that OsHKT4::GUS appeared strong GUS activity, expressed mainly in vascular ti...
Article
Full-text available
The field-based experiment was conducted during the 2020 – 2021 cropping season to evaluate the response of two types of bean (Vicia faba L.): Luz De Otono (C1) and Aquadulce (C2 for different spray treatments namely, control (T1), 40mg IAA.L-1 (T2) and 10gm of licorice with yeast.L-1 (T3),. The treatments were assigned to experimental plots follow...
Article
Full-text available
A field experiment was conducted at the first station of the College of Agriculture/University of Al-Muthanna for the agricultural season 2020-2021, with the aim of studying the effect of treatment with salinity stress and kinetin on the growth and yield of the wheat ( Triticum aestivum L.) cultivar of Bohooth 22. The experiment was applied by spli...
Article
Full-text available
A field experiment was carried out at the Agricultural Research and Experiments Station of the College of Agriculture, Al-Muthanna University during the summer season 2020. Pots of 15 cm diameter were used for planting the genotypes, with (SS, F-RCBD) and three replications. Ten different genotypes rice were used (FRI, FR2, FR15, FR16, FR17, FR18,...
Article
Full-text available
A field experiment was carried out in Samawa desert (70 km west of Samawa city, Al-Muthanna governorate), during the 2018-2019 and 2019-2020 agricultural seasons, to study the response of three Iraqi cultivars of wheat (Tamooz2, Ibaa99, Abu Ghraib3) to three planting dates (November 15, 1 st December and December 15) under Samawa desert conditions....
Book
Full-text available
Although there are thousands of plant species growing on the surface of the globe, whether wild or cultivated, the number of cultivated plant species is very little, of which have been distinguished and identified with 287,655 plant species, of which 258,650 flowering plants and more than 15,000 of the bryophyte until now, and that 50% of the world...
Article
Full-text available
Four genotypes of bread wheat were used to study the quantitative traits (grain yield per plant, No. of spikes per plant, No. of grains per spike, weight of 1000 grains, harvest index and biology yield) and growth traits (plant height, flag leaf area, and No. of tillers per plant).The study included estimation of genetic and phenotypic correlation...
Article
Full-text available
Four genotypes of bread wheat were used to study the quantitative traits (grain yield per plant, No. of spikes per plant, No. of grains per spike, weight of 1000 grains, harvest index and biology yield) and growth traits (plant height, flag leaf area, and No. of tillers per plant). The study included estimation of genetic and phenotypic correlation...
Article
Full-text available
The experiment was carried out at the second research station, College of Agriculture, Al-Muthanna University during the winter season 2018-2019, to investigate the performance of four wheat cultivars namely Rasheed, Iba99, Bhooth and Abu Graib subjected K+ supplants to antagonize Na+ effect on growth, yield and genome of four wheat cultivars. Subs...
Article
Full-text available
In rice (Oryza sativa 'Nipponbare'), the genes family HKT is composed of eight members. It encodes for Na + transporters that play an important role in salt stress tolerance. Functional analysis, it has been determined that there is a wide diversity of these transporters in terms of their Na + /K + selectivity and K + affinity, in addition, plays a...
Article
Abstract In rice (Oryza sativa ‘Nipponbare’), the genes family HKT is composed of eight members. It encodes for Na+ transporters that play an important role in salt stress tolerance. Functional analysis, it has been determined that there is a wide diversity of these transporters in terms of their Na+/K+ selectivity and K+ affinity, in addition, pla...
Article
Full-text available
Abstract This experiment was conducted to investigate some physiological and molecular mechanisms involved in the genotypic difference of K+ uptake and salinity tolerance between Shaker OsK4.1 and OsK4.3 genes under salt stress (50 mM NaCl) conditions. Using genotypic analysis by double PCR, we identified 3 plants WT, 6 homozygous and 9 heterozygou...
Thesis
Plant adaptation to salinity stress: characterisation of ecotypic variants and knock-out lines for Na+ transport systems in rice The salinity of irrigation water and soils is one of the main abiotic constraints in agriculture. Among the 130 mha of rice grown around the world, about 30% are in areas where salinity is too high to allow for good yiel...
Article
Full-text available
Salinity is a major factor limiting crop productivity. Rice (Oryza sativa), a staple crop for the majority of the world, is highly sensitive to salinity stress. To discover novel sources of genetic variation for salt tolerance-related traits in rice, we screened 390 diverse accessions under 14 days of moderate (9 dS·m-1) salinity. In this study, sh...
Data
GWA of root K+ content in salt conditions. Genome-wide association (GWA) was performed using a mixed model that accounted for population structure and relatedness between accessions of RDP1 using 365 accessions of RDP1 and 397,812 SNPs. For each trait the least squares mean was used as the dependent variable. The red horizontal line indicates a sta...
Data
GWA of root biomass traits. (A) Root biomass in control conditions; (B) root biomass in saline conditions; (C) root biomass response. Genome-wide association (GWA) was performed using a mixed model that accounted for population structure and relatedness between accessions of RDP1 using 365 accessions of RDP1 and 397,812 SNPs. The red horizontal lin...
Data
Haplotype blocks for the region spanning 30.6–30.7 Mb on chromosome 4. The heavy black line indicates the boundaries of individual blocks, which were determined using the 4Gamete rule in Haploview with a recombination threshold of > 2%. The track above the heatmap indicates the position of the SNP within the defined region. Each cell represents the...
Data
Haplotype blocks for the region spanning 30.8–30.9 Mb on chromosome 4. The heavy black line indicates the boundaries of individual blocks, which were determined using the 4Gamete rule in Haploview with a recombination threshold of > 2%. The track above the heatmap indicates the position of the SNP within the defined region. Each cell represents the...
Data
Expression of HKT1;1 and HKT1;4 in root and shoot tissue. RNA sequencing of root and shoot tissue was performed with two accessions of RDP1 at 10 days after transplanting (12 day old plants) when the first tiller was visibly emerging. The expression levels of HKT1;1 and HKT1;4 are expressed as reads per kilobase per million mapped reads (RPKM). RPK...
Data
HKT1;1-Ni and HKT1;1-Zh display high selectivity for Na+ against other monovalent cations. (A-D) Oocytes expressing HKT1;1-Ni (A, C) or HKT1;1-Zh (B, D) were successively bathed with solutions containing 50 mmoles•l-1 of different monovalent cations (Na+, K+, Li+, Rb+, or Cs+, as chloride salts)(A, B) or different combinations of Na+ and K+ concent...
Data
Genetic correlation analysis of root and shoot ion traits. Restricted maximum likelihood implemented in ASReml-R v.3.0 was used to estimate genetic variances and covariances among traits, which were used to calculate genetic correlations among traits [66]. Here, population structure and polygenic effects were not included in the mixed linear model...
Data
Pearson correlation of eight phenotypic traits performed across all subpopulations in RDP1 (n = 383). SB and RB indicate the ratio of biomass in salt to control for shoots and roots, respectively. Highlighted cells indicate a statistically significant relationship (p < 0.05). The number within each cell indicates Pearson’s correlation coefficient....
Data
Pearson correlation of eight phenotypic traits performed within the admix subpopulation (n = 53). SB and RB indicate the ratio of biomass in salt to control for shoots and roots, respectively. Highlighted cells indicate a statistically significant relationship (p < 0.05). The number within each cell indicates Pearson’s correlation coefficient. (XLS...
Data
Haplotype blocks for the region spanning 30.48–30.6 Mb on chromosome 4. The heavy black line indicates the boundaries of individual blocks, which were determined using the 4Gamete rule in Haploview with a recombination threshold of > 2%. The track above the heatmap indicates the position of the SNP within the defined region. Each cell represents th...
Data
Expression of HKT1;1 (A) and HKT1;4 (B) in RNAi plants. For HKT1;1, gene expression was quantified in whole shoot tissue in four-day-old T1 plants using real-time PCR, while for HKT1;4 expression was quantified from flag leaf tissue at anthesis. All expression is expressed relative to developmentally identical ‘Kitaake’ plants using the using the d...
Data
Transcripts within RNC4 displaying differences in expression between allelic groups at SNP-4-30535352 (XLSX)
Data
GWA of shoot biomass traits. (A) Shoot biomass in control conditions; (B) shoot biomass in saline conditions; (C) shoot biomass response. Genome-wide association (GWA) was performed using a mixed model that accounted for population structure and relatedness between accessions of RDP1 using 365 accessions of RDP1 and 397,812 SNPs. The red horizontal...
Data
Shoot and root growth in RNAi lines. T2 RNAi lines of HKT1;1 (A,B) and HKT1;4 (C,D) were exposed to 14d of 9 dS m-1. Biomass was significantly reduced by salt treatment in all lines (p < 0.05). Error bars represent standard error of the mean where n = 12–20 plants. Kitaake was used as a WT control in all experiments. (TIF)
Data
Position and conservation of non-synonymous variants in HKT1;1.2. (A) Secondary structure of OsHKT1;1 polypeptide showing the position of AA changes, as exemplified between ‘Nipponbare’ and ‘Zhenshan 2’ variants. (B) Protein alignment of OsHKT1;1.2 and HKT1;1 homologs from various species. Black triangles indicate position of three non-synonymous m...
Data
HKT1;1-Ni and HKT1;1-Zh display similar affinity for Na+. (A, B) Current voltage (I-V) relationships determined in solutions containing 3 to 100 mM Na+-glutamate in HKT1;1-Ni (A) or HKT1;1-Zh (B) expressing oocytes from the same batch. (C) HKT1;1-Ni or -Zh inward conductance plotted versus external Na+ concentration. Whole oocyte HKT1;1 inward cond...
Data
List of primer sequences used for gene expression analysis, cloning and SNP confirmation. (XLSX)
Data
GWA of shoot ion traits in salt conditions. Genome-wide association (GWA) was performed for (A) Na+ content, (B) K+ content, and (C) Na+:K+ using a mixed model that accounted for population structure and relatedness between accessions of RDP1 using 365 accessions of RDP1 and 397,812 SNPs. For each trait the least squares mean was used as the depend...
Data
Haplotype blocks for the region spanning 30.9–31.06 Mb on chromosome 4. The heavy black line indicates the boundaries of individual blocks, which were determined using the 4Gamete rule in Haploview with a recombination threshold of > 2%. The track above the heatmap indicates the position of the SNP within the defined region. Each cell represents th...
Data
Comparisons of nucleotide diversity between cultivated and wild rice in the region surrounding HKT1;1. The three non-synonymous SNPs are highlighted in red. The y-axis represents the ratio of the nucleotide diversity (pi) in Oryza rufipogon to Oryza sativa japonica or Oryza sativa indica. (TIF)
Data
Broad-sense heritability (H2) estimates for the ten traits recorded. Broad sense-heritability was determined on an entry-mean basis using all 390 accessions (383 from RDP1 and seven check varieties). Period indicates the time of year the experiment was conducted (i.e. June-July or Aug-Sept), and genotype indicates the accession (XLSX)
Data
Pearson correlation of eight phenotypic traits performed within the tropical japonica subpopulation (n = 84). SB and RB indicate the ratio of biomass in salt to control for shoots and roots, respectively. Highlighted cells indicate a statistically significant relationship (p < 0.05). The number within each cell indicates Pearson’s correlation coeff...
Data
Pearson correlation of eight phenotypic traits performed within the indica subpopulation (n = 77). SB and RB indicate the ratio of biomass in salt to control for shoots and roots, respectively. Highlighted cells indicate a statistically significant relationship (p < 0.05). The number within each cell indicates Pearson’s correlation coefficient. (XL...
Data
Least-squares means for the ten phenotypic traits recorded for 390 accessions. (XLSX)
Data
QTL locations for the eight phenotypic traits and the percent variation explained by the most significant SNP for each QTL. (XLSX)
Data
High-confidence genetic variants (at least 10x coverage) found in the ORFs of genes within RNC4. (XLSX)
Data
Pearson correlation of eight phenotypic traits performed within the aus subpopulation (n = 52). SB and RB indicate the ratio of biomass in salt to control for shoots and roots, respectively. Highlighted cells indicate a statistically significant relationship (p < 0.05). The number within each cell indicates Pearson’s correlation coefficient. (XLSX)
Data
Pearson correlation of eight phenotypic traits in the temperate japonica subpopulation (n = 91). SB and RB indicate the ratio of biomass in salt to control for shoots and roots, respectively. Highlighted cells indicate a statistically significant relationship (p < 0.05). The number within each cell indicates Pearson’s correlation coefficient. (XLSX...
Book
Full-text available
MILS-16 provides an up-to-date review of the impact of alkali metal ions on life. Their bioinorganic chemistry and analytical determination, the solid state structures of bio-ligand complexes and the properties of alkali metal ions in solution in the context of all kinds of biologically relevant ligands are covered, this includes proteins (enzymes)...
Book
The two alkali cations Na(+) and K(+) have similar relative abundances in the earth crust but display very different distributions in the biosphere. In all living organisms, K(+) is the major inorganic cation in the cytoplasm, where its concentration (ca. 0.1 M) is usually several times higher than that of Na(+). Accumulation of Na(+) at high conce...
Chapter
The two alkali cations Na+ and K+ have similar relative abundances in the earth crust but display very different distributions in the biosphere. In all living organisms, K+ is the major inorganic cation in the cytoplasm, where its concentration (ca. 0.1 M) is usually several times higher than that of Na+. Accumulation of Na+ at high concentrations...
Chapter
Full-text available
The two alkali cations Na(+) and K(+) have similar relative abundances in the earth crust but display very different distributions in the biosphere. In all living organisms, K(+) is the major inorganic cation in the cytoplasm, where its concentration (ca. 0.1 M) is usually several times higher than that of Na(+). Accumulation of Na(+) at high conce...
Article
Path coefficient analysis for some field properties and grain yield of several rice genotypes(Oryza sativa L.) Mahmood Abdul Razzaq Hanoosh Fouad Razzaq Abdul Hussein Technical Institute _ Najaf College of Agri._Univ. of Al muthna Abstract Afield Experiments were carried out at field of Al-Mushkab during 2007 to study path coefficient for many r...
Article
Full-text available
Abstract Field experiment was contacted in Al-Muthanna province/ region of Najmi district during the agricultural seasons (2009-2010) to know Response of two aromatic hybrid of rice for foliar feeding with foamed sulfur (0, 25,50,75 S/L) on growth and yield of tow hybrid from rice (Anber-33×Jassmin) and (Anber-33×Sentral) which was obtained from hy...

Questions

Question (1)
Question
Hi dears,
If I have a Nano- Potassium in liquid (CC), i mean volume
And I want treated my plants with Nano- Potassium foliar treatments with concentrations of (2/1000 and 4/1000)
What amount of Nano- Potassium can I put in water ?
In order to get (2/1000 and 4/1000)
For example :
20 cc of Nano- Potassium in liquid is placed in one liter of water ... etc.
Thank u so much for all response

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Projects

Projects (6)
Project
This study aims to use CRISPR/Cas9 technology in japonica rice, cv Pokali (tolerant to salinity) and isolating the HKT genes from it and transferring them to the Iraqi rice, cv Anber-33 (Which is non-tolerant to salinity relatively) in order to obtain the genotypes of Anber-33 tolerant to highly saline stress and adapted it to the conditions of the Iraqi soil.
Project
Determine the role of TaHKT2;2 gene responsible to salt stress tolerance in bread wheat