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Publications (28)
Root-knot nematode (RKN) Meloidogyne spp. is one of the most damaging parasites due to its wide range of hosts. Here, we report a C. elegans receptor DAF-12 ortholog gene in Meloidogyne incognita (DAF-12 Minc ), a promising molecular target to modify the RKN life cycle. Using a combination of Hidden Markov Models (HMM) based sequence search and phy...
La provincia de El Oro es la zona minera de oro más importante del Ecuador, pero la actividad ha causado daños al ecosistema y a la salud humana, debido al vertimiento de residuos de extracción cargados de metales pesados al río Calera, que es afluente con el río Amarillo del río Puyango. Este trabajo presenta los resultados de la evaluación de la...
In view of the recurrent applications of pesticides in agricultural producing countries, the increased presence of these substances in the environment raise a demand for the evaluation of adverse effects on non-target organisms. This study assesses the impact of exposure to five pesticides suspected of being endocrine disruptors (atrazine, 2,4-dich...
El nematodo Caenorhabditis elegans es capaz de detectar señales químicas externas y realizar en consecuencia comportamientos de quimiotaxis. Este comportamiento refleja las funciones del sistema nervioso para recibir, procesar, integrar e interpretar señales sensoriales para generar las respuestas apropiadas. El isoamilalcohol (IAA) es un líquido i...
The development of environmentally friendly new procedures for the synthesis of metallic nanoparticles is one of the main goals of nanotechnology. Proteins and enzymes from plants, filamentous fungi, yeast, and bacteria to produce nanoparticles are both valuable and viable alternatives to conventional synthesis of nanomaterials due to their high ef...
The suitability of the yeast Schwanniomyces (f. Debaryomyces) occidentalis to produce phytase activity on a Smallanthus sonchifolius (yacon) extract-based medium (YSM) was studied.
Optimal conditions for phytase production were evaluated in YSM at 28 °C and pH 5.5, with an inoculum of 10⁷ cells/mL, in shaken flasks, using a Box-Benkhen optimization...
Since glyphosate became one of the most used herbicides worldwide, its constant release into the environment has raised concern among the general public and international regulatory agencies. Traces of this compound have been detected in fresh and processed food and in environmental matrices. Although there is still controversy about the impact of...
La inclusión de los análisis ecotoxicológicos en los estudios ambientales resulta indispensable ya que aporta información sobre los efectos que los contaminantes puedan causar en los organismos expuestos y en los ecosistemas receptores. El objetivo del trabajo fue evaluar la calidad del agua en la cuenca del arroyo Cañuelas (Buenos Aires, Argentina...
This work aimed to evaluate the effect of ozone washing (maximum concentration 3.5 mgL-1- 5 and 15 min) on Botrytis cinerea decay, physicochemical parameters, bioactive compounds, in vitro and in vivo antioxidant activity, and sensory properties of strawberries cv. Albion throughout refrigerated storage at 5 ± 1 °C. A 5 min long ozonation delayed t...
The stink bug Nezara viridula is one of the most threatening pests for agriculture in North and South America, and its oral secretion may be responsible for the damage it causes in soybean (Glycine max) crop. The high level of injury to seeds caused by pentatomids is related to their feeding behavior, morphology of mouth parts, and saliva, though i...
La inclusión de los análisis ecotoxicológicos en los estudios ambientales resulta indispensable ya que aporta información sobre los efectos que los contaminantes puedan causar en los organismos expuestos y en los ecosistemas receptores. El objetivo del trabajo fue evaluar la calidad del agua en la cuenca del arroyo Cañuelas (Buenos Aires, Argentina...
Synthesis of noble metal nanoparticles using natural products and living organisms has drawn a lot of interest owing to economic prospects and potential applicability in different fields. For this work we used the exudate of the soil fungus Macrophomina phaseolina for a low-cost method of green synthesis to obtain stable silver-silver chloride nano...
La cuenca del Arroyo Pergamino fue seleccionada por ser un área representativa de la producción intensiva de soja, así como del aumento de las actividades urbanas que afectan al recurso hídrico. Se realizaron muestreos en 20 puntos seleccionados, entre julio de 2014 y febrero de 2015, coincidentes con la campaña de cultivo de soja. Se analizaron di...
The presence of a single surface plasmon resonance peak at 415 nm in the scanned UV-vis region suggests the nanoparticles have spherical shape, which was further confirmed by electron microscopy. A drop of Ag/AgCl-NPs sample solution synthesized by Macrophomina phaseolina cell-free filtrate was deposited onto a silicon wafer to analyze the morpholo...
Due to intensive agricultural activities to meet the growing needs for food, large volumes of water are consumed and an increasing amount of agrochemicals are released into the environment threatening the aquatic ecosystem. In order to ensure a sustainable agricultural management, it is crucial to develop an integrated water assessment plan that in...
In the pathogen Candida albicans protein kinase A (PKA) catalytic subunit is encoded by two genes TPK1 and TPK2 and the regulatory subunit by one gene, BCY1. PKA mediates several cellular processes such as cell cycle regulation and the yeast to hyphae transition, a key factor for C. albicans virulence. The catalytic isoforms Tpk1p and Tpk2p share r...
The yeast Yarrowia lipolytica accumulates oils and is able to produce extracellular lipases when growing in different carbon sources including glycerol, the principal by-product of the biodiesel industry. In this study, biomass production of a novel mutant strain of Y. lipolytica was statistically optimized by Response Surface Methodology in media...
Candida albicans undergoes a reversible morphological transition from single yeast cells to pseudohyphal and hyphal filaments. In this organism, cAMP-dependent protein kinase (PKA), coded by two catalytic subunits (TPK1 and TPK2) and one regulatory subunit (BCY1), mediates basic cellular processes, such as the yeast-to-hypha transition and cell cyc...
Candida albicans cAMP-dependent protein kinase (PKA) is coded by two catalytic subunits (TPK1 and TPK2) and one regulatory subunit (BCY1). In this organism the cAMP/PKA signalling pathway mediates basic cellular processes, such as the yeast-to-hyphae transition and cell cycle regulation. In the present study, we investigated the role of C. albicans...
Previous studies on the dimorphic transition of Yarrowia lipolytica suggested opposite roles for MAPK and PKA pathways in this phenomenon. To obtain conclusive evidences for these opposite roles we isolated and disrupted the unique gene encoding the Pka catalytic subunit (TPK1). TPK1 was regulated only at the post-transcriptional level, with Pka ac...
To study the effect of prestress conditions on the freezing and thawing (FT) response of two baker's yeast strains and the use of statistical analysis to optimize resistance to freezing.
Tolerance to FT of industrial strains of Saccharomyces cerevisiae was associated to their osmosensitivity and growth phase. Pretreatments with sublethal stresses [...
Thermal transitions were analyzed after freezing and thawing of two commercial baker's yeast cells of Saccharomyces cerevisiae having high and low tolerance to sugar. Yeast in stationary phase of growth were exposed to two cycles of freezing (20 h and 4 h) at −20 °C after being exposed to various physical and chemical mild stresses and differential...
