Florence Poulletier de Gannes

Université Bordeaux 1 · UMR IMS - Laboratoire d'Integration du Materiau au Système (IMS)

Continuous monitoring of glucose levels has improved diabetes therapy. Current approaches rely on enzyme-linked electrochemical probes but do not allow a fully autonomous artificial pancreas. In contrast, monitoring the activity of a few electrogenic pancreatic islets in a biosensor may harness the computational power of the different endocrine cel...

Whether human cells are impacted by environmental electromagnetic fields (EMF) is still a matter of debate. With the deployment of the fifth generation (5G) of mobile communication technologies, the carrier frequency is increasing and the human skin becomes the main biological target. Here, we evaluated the impact of 5G‐modulated 3.5 GHz radiofrequ...

Introduction The current deployment of the fifth generation (5G) of wireless communications raises new questions about the potential health effects of exposure to radiofrequency (RF) fields. So far, most of the established biological effects of RF have been known to be caused by heating. We previously reported inhibition of the spontaneous electric...

The potential health risks of exposure to radiofrequency electromagnetic fields from mobile communications technologies have raised societal concerns. Guidelines have been set to protect the population (e.g. non-specific heating above 1 °C under exposure to radiofrequency fields), but questions remain regarding the potential biological effects of n...

Whether ion channels experience ligand-dependent dynamic ion selectivity remains of critical importance since this could support ion channel functional bias. Tracking selective ion permeability through ion channels, however, remains challenging even with patch-clamp electrophysiology. In this study, we have developed highly sensitive bioluminescenc...

Previous studies have shown that spontaneously active cultured networks of cortical neuron grown planar microelectrode arrays are sensitive to radiofrequency (RF) fields and exhibit an inhibitory response more pronounced as the exposure time and power increase. To better understand the mechanism behind the observed effects, we aimed at identifying...

Cardiac arrhythmias represent about 50% of the cardiovascular diseases which are the first cause of mortality in the world. Implantable medical devices play a major role for treating these arrhythmias. Nevertheless the leads induce an unwanted biological phenomenon called fibrosis. This phenomenon begins at a cellular level and is effective at a ma...

Previous studies have shown that spontaneously active cultured networks of cortical neuron grown planar microelectrode array are sensitive to radiofrequency (RF) fields and exhibit an inhibitory response more pronounced as the exposure time and power increase. To better understand the mechanism behind the observed effects, we aimed at identifying s...

Previous studies have shown that spontaneously active cultured networks of cortical neuron grown planar microelectrode arrays are sensitive to radiofrequency (RF) fields and exhibit an inhibitory response more pronounced as the exposure time and power increase. To better un-derstand the mechanism behind the observed effects, we aimed at identifying...

This study aims to analyze in real‐time the potential modifications induced by low‐level continuous‐wave and Global System for Mobile Communications radiofrequency (RF) exposure at 1.8 GHz on brain activation in anesthetized mice. A specific in vivo experimental setup consisting of a dipole antenna for the local exposure of the brain was fully char...

It remains controversial whether exposure to environmental radiofrequency signals (RF) impacts cell status or response to cellular stress such as apoptosis or autophagy. We used two label-free techniques, cellular impedancemetry and Digital Holographic Microscopy (DHM), to assess the overall cellular response during RF exposure alone, or during co-...

In this article, a mode-stirred reverberation chamber (RC) was designed and proposed for the first time as a cell culture incubator for in vitro electromagnetic waves exposure of adherent cells in tissue culture plates (TCPs). Typical cell incubators require specific conditions, such as temperature of 37 °C and humidity rate of 95%, which are chall...

In this study, we present a first-of-a-kind biological-hardware-software tool to evaluate the physiological condition of in vitro myotubes in response to electrical stimulation. We demonstrate that impedance spectroscopy on a microelectrode array can testify for physiological changes of muscle cells under electrical stimulation. The platform is des...

Ion channels are attractive drug targets for many therapeutic applications. However, high-throughput screening (HTS) of drug candidates is difficult and remains very expensive. We thus assessed the suitability of the Bioluminescence Resonance Energy Transfer (BRET) technique as a new HTS method for ion-channel studies by taking advantage of our rec...

As of today, only acute effects of RF fields have been confirmed to represent a potential health hazard and they are attributed to non-specific heating (≥ 1 °C) under high-level exposure. Yet, the possibility that environmental RF impact living matter in the absence of temperature elevation needs further investigation. Since HSF1 is both a thermose...

Purpose: The present study was conducted to re-evaluate the effect of low-level 1800 MHz RF signals (up to public exposure level for local exposure) on RAS/MAPK activation in live cells. Material and methods: Using molecular probes based on the Bioluminescence Resonance Energy Transfer technique (BRET), we assessed the effect of Continuous wave (CW...

Aim: The Pasche research group has reported that tumor-specific electromagnetic field frequencies have physiological and potential anti-tumor effects in cells, animals, and humans. Our aim was to investigate whether these fields have similar effects on physiological parameters in murine tumor models. Methods: Human HuH7 or HEPG2 cells were implante...

So far, the only identified biological effects of radiofrequency fields (RF) are known to be caused by heating but the issue of potential nonthermal biological effects, especially on the central nervous system (CNS), remains open. We previously reported a decrease in the firing and bursting rates of neuronal cultures exposed to a Global System for...

Blood-brain barrier (BBB) permeation and neuron degeneration were assessed in the rat brain following exposure to mobile communication radiofrequency (RF) signals (GSM-1800 and UMTS-1950). Two protocols were used: (i) single 2 h exposure, with rats sacrificed immediately, and 1 h, 1, 7, or 50 days later, and (ii) repeated exposures (2 h/day, 5 days...

The existence of effects of radiofrequency field exposure at environmental levels on living tissues and organisms remains controversial, in particular regarding potential "nonthermal" effects produced in the absence of temperature elevation. Therefore, we investigated whether TRPV1, one of the most studied thermosensitive channels, can be activated...

Multiplexed bioluminescence resonance energy transfer (BRET) assays were developed to monitor the activation of several functional transient receptor potential (TRP) channels in live cells and in real time. We probed both TRPV1 intramolecular rearrangements and its interaction with Calmodulin (CaM) under activation by chemical agonists and temperat...

The present study focused on gap junctional intercellular communication (GJIC) as a target for biological effects of extremely low-frequency (ELF) magnetic field (MF) exposure. Fluorescence recovery after photobleaching microscopy (FRAP) was used to visualize diffusion of a fluorescent dye between NIH3T3 fibroblasts through gap junctions. The direc...

The central nervous system is the most likely target of mobile telephony radiofrequency (RF) field exposure in terms of biological effects. Several electroencephalography (EEG) studies have reported variations in the alpha-band power spectrum during and/or after RF exposure, in resting EEG and during sleep. In this context, the observation of the s...

The bioeffects of exposure to Wireless High-Fidelity (WiFi) signals on the developing nervous systems of young rodents was investigated by assessing the in vivo and in situ expression levels of three stress markers: 3-Nitrotyrosine (3-NT), an oxidative stress marker and two heat-shock proteins (Hsp25 and Hsp70). These biomarkers were measured in th...

An experimental approach was used to assess immunological biomarkers in the sera of young rats exposed in utero and postnatal to non-ionizing radiofrequency fields. Pregnant rats were exposed free-running, 2 h/day and 5 days/week to a 2.45 GHz Wi-Fi signal in a reverberation chamber at whole-body specific absorption rates (SAR) of 0, 0.08, 0.4, and...

The increase in exposure to the Wireless Fidelity (Wi-Fi) wireless communication signal has raised public health concerns especially for young people. Animal studies looking at the effects of early life and prenatal exposure to this source of electromagnetic fields, in the radiofrequency (RF) range, on development and behavior have been considered...

Animal studies can contribute to addressing the issue of possible greater health risk for children exposed to 50-60 Hz extremely low frequency (ELF) magnetic fields (MFs), mostly in terms of teratological effects and cancer. Teratology has been extensively studied in animals exposed to ELF MFs but experiments have not established adverse developmen...

The impact of Wi-Fi exposure was investigated on the developing immune system of C57BL/6 mice, whole-body exposed 2-hour/day for 7 weeks at 2.45 GHz. The pups were exposed for 2 weeks in utero and then for 5 weeks post-natal. The free-running animal exposure system was a reverberation chamber with SAR levels of 0, 0.8, 0.4, and 4 W/kg. There was no...

The present study aimed, for the first time, at evaluating the consequences of in utero exposure to a Wi-Fi signal on pregnant rats and their pups. SAR levels of 0.08, 0.4, and 4 W/kg were used. Prenatal study of foetuses delivered by caesarean was carried out on 5 females/group. Maternal observations after delivery and offspring follow-up were don...

The effects of RF radiation were investigated on the rat reproductive system in compliance with the OECD protocole-421. Free moving male and female rats (before and during pregnancy) were whole-body sham exposed or exposed to a Wi-Fi signal (2450 MHz) using a reverberation chamber at WB SAR of 0.1 or 4 W/kg. Preliminary results show that body-weigh...

In this study we investigated the effect of the Enhanced Data rate for GSM Evolution (EDGE) signal on cells of three human brain cell lines, SH-SY5Y, U87 and CHME5, used as models of neurons, astrocytes and microglia, respectively, as well as on primary cortical neuron cultures. SXC-1800 waveguides (IT'IS-Foundation, Zürich, Switzerland) were modif...

Pregnant rats were daily whole-body exposed or sham-exposed to a Wi-Fi signal in a free-running reverberation chamber at 0, 0.08, 0.4, and 4 W/kg for 2 h during the last 2 weeks of gestation (5 days/week). Following this in utero exposure, the pups were divided into two groups and 1 group continued exposure for 5 weeks after birth. Several brain ar...

Few studies have shown that local exposure to radiofrequency electromagnetic fields (RF) induces intensity-dependent physiological changes, especially in the brain. The aim of the present study was to detect reproducible responses to local RF exposure in the parietal cortex of anesthetized rats and to determine their dependence on RF intensity. The...

There is some concern that exposure to extremely low-frequency magnetic fields (MF) causes adverse health effects via signal transduction pathways. Two previous studies reported that exposure to 50-Hz MF decreased the binding affinity of the 1B receptor subtype of serotonin (5-HT) in rat brain membranes. The aim of this study was to investigate whe...

INTRODUCTION In recent decades, concern has been growing about decreasing fecundity and fertility in the human population [1]. Several studies indicate that semen quality may have decreased, associated with reduced fertility [2], but few potential factors have been identified. Exposures to non-ionizing electromagnetic fields, especially RF fields u...

Pregnant Wistar rats were exposed in a reverberation chamber to Wi-Fi signals (whole-body SAR 0.08, 0.4, 4 W/kg) from gestational day 3 to 20. The presence of stress markers was screened in the blood and brain of pups at day 2, and months 1, 2, and 3 after birth. At day 2, no genotoxic effect was detected in the blood. Whatever SAR and age, there w...

There is some evidence from epidemiological studies of an association between occupational exposure to electromagnetic fields and Amyotrophic Lateral Sclerosis (ALS). Our aim was to perform, for the first time, an animal study in a controlled magnetic environment. We used the SOD-1 mouse model to assess the possible effect of ELF magnetic fields on...

In a series of Russian and Ukrainian papers published from 1974-1986, it was reported that 30-day whole-body exposures to continuous-wave (CW) radiofrequency (RF) radiation at 2375 MHz and 5 W/m(2) disrupted the antigenic structure of rat brain tissue. The authors suggested that this action caused an autoimmune response in exposed animals. Moreover...

Salford et al. reported in 2003 that a single 2-h exposure to GSM-900 mobile telephony signals induced brain damage (increased permeability of the blood-brain barrier and presence of dark neurons) 50 days after exposure. In our study, 16 Fischer 344 rats (14 weeks old) were exposed head-only to the GSM-900 signal for 2 h at various brain-averaged S...

An increase in Ornithine Decarboxylase (ODC) activity was reported in L929 murine fibroblast cells after exposure to a digital cellular telephone signal. This result was not confirmed by several other studies, including the one reported in a companion paper. As a partner in the Perform-B programme, we extended this study to human neuroblastoma cell...

We conducted a systematic review of scientific studies to evaluate whether the use of wireless phones is linked to an increased incidence of the brain cancer glioma or other tumors of the head (meningioma, acoustic neuroma, and parotid gland), originating in the areas of the head that most absorb radiofrequency (RF) energy from wireless phones. Epi...

L’activation de la voie de l’indolamine 2,3-dioxygénase est connue pour être présente dans un grand nombre de maladies neurodégénératives, telles que la sclérose latérale amyotrophique, les maladies d’Alzheimer et de Parkinson, la sclérose en plaques. Cette enzyme inductible est à l’origine de la production de métabolites neurotoxiques. Dans notre...

We previously reported the inability of Global System for Mobile communication (GSM) signals at 900 (GSM-900) and 1800 (GSM-1800) MegaHertz (MHz) to induce morphological and physiological changes in epidermis of Hairless rats. The present work aimed at investigating heat shock proteins (HSP) expression--as a cellular stress marker--in the skin of H...

NO and ONOO- are involved in neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer disease, and multiple sclerosis. The direct quantification of these radical species in vivo is not relevant because not technically reliable. To overcome these difficulties, the identification of circulating antibodies directed against NO- and N...