Florence Constantinesco

Florence Constantinesco
Université Paris-Sud 11 | Paris 11 · Institut de Génétique et Microbiologie (IGMORS)

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9
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Publications

Publications (9)
Article
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The ubiquitous Rad50 and Mre11 proteins play a key role in many processes involved in the maintenance of genome integrity in Bacteria and Eucarya, but their function in the Archaea is presently unknown. We showed previously that in most hyperthermophilic archaea, rad50-mre11 genes are linked to nurA encoding both a single-strand endonuclease and a...
Article
Full-text available
We showed previously that rad50 and mre11 genes of thermophilic archaea are organized in an operon‐like structure with a third gene (nurA) encoding a 5′ to 3′ exonuclease. Here, we show that the rad50, mre11 and nurA genes from the hyperthermo philic archaeon Sulfolobus acidocaldarius are co‐transcribed with a fourth gene encoding a DNA helicase. T...
Article
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We isolated and characterized a new nuclease (NurA) exhibiting both single-stranded endonuclease activity and 5'-3' exonuclease activity on single-stranded and double-stranded DNA from the hyperthermophilic archaeon Sulfolobus acidocaldarius. Nuclease homologs are detected in all thermophilic archaea and, in most species, the nurA gene is organized...
Article
The structural gene TRM1 encoding tRNA(guanine 26, N (2), N (2))-dimethyltransferase (Trm1p) of the hyperthermophilic archaeon Pyrococcus furiosus was cloned and expressed in Escherichia coli. The corresponding recombinant enzyme (pfTrm1p) with a His6-tag at the N terminus was purified to homogeneity in three steps. The enzyme has a native molecula...
Article
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The modification patterns of in vitro transcripts of two yeast Saccharomyces cerevisiae tRNAs (tRNAPhe and tRNAAsp) and one archaeal Haloferax volcanii tRNA (tRNAIle) were investigated in the cell-free extract of Pyrococcus furiosus supplemented with S-adenosyl-l-methionine (AdoMet). The results indicate that the enzymatic formation of 11 distinct...
Article
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The structural gene pfTRM1 ( GenBank accession no. AF051912) , encoding tRNA(guanine-26,N2-N2) methyltransferase (EC 2.1.1.32) of the strictly anaerobic hyperthermophilic archaeon Pyrococcus furiosus, has been identified by sequence similarity to the TRM1 gene of Saccharomyces cerevisiae (YDR120c). The pfTRM1 gene in a 3.0 kb restriction DNA fragme...
Article
Inosine (6-deaminated adenosine) is a characteristic modified nucleoside that is found at the first anticodon position (position 34) of several tRNAs of eukaryotic and eubacterial origins, while N1-methylinosine is found exclusively at position 37 (3' adjacent to the anticodon) of eukaryotic tRNA(Ala) and at position 57 (in the middle of the psi lo...
Article
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Transfer RNAs of the extreme halophile Haloferax volcanii contain several modified nucleosldes, among them 1-methylpseudouridine (m1ψ), pseudouridlne (ψ), 2′-0-methylcytosine (Cm) and 1-methylinosine (m1l), present in positions 54, 55, 56 and 57 of the ψ-loop, respectively. At the same positions in tRNAs from eubacteria and eukaryotes, ribothymidln...

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