Fleur E Mason

Fleur E Mason
Universitätsmedizin Göttingen · Department of Pharmacology

MA, PhD

About

18
Publications
2,653
Reads
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550
Citations
Additional affiliations
May 2017 - present
Universitätsmedizin Göttingen
Position
  • PhD Student
September 2013 - February 2016
Universitätsmedizin Göttingen
Position
  • PhD Student
September 2009 - present
Position
  • Cardiac Electrophysiology, epifluorescent calcium measurement, confocal microscopy
Education
October 2009 - September 2013
The University of Manchester
Field of study
  • Cardiac Physiology
October 2002 - June 2005
University of Oxford
Field of study
  • Physiological Sciences

Publications

Publications (18)
Article
Full-text available
Dilated cardiomyopathy (DCM) is a major risk factor for heart failure and is associated with the development of life-threatening cardiac arrhythmias. Using a patient-specific induced pluripotent stem cell-derived cardiomyocyte (iPSC-CM) model harbouring a mutation in cardiac troponin T (R173W), we aim to examine the cellular basis of arrhythmogenes...
Article
Full-text available
Background: Phosphodiesterases (PDE) critically regulate myocardial cAMP and cGMP levels. PDE2 is stimulated by cGMP to hydrolyze cAMP, mediating a negative crosstalk between both pathways. PDE2 upregulation in heart failure contributes to desensitization to β-adrenergic overstimulation. After isoprenaline (ISO) injections, PDE2 overexpressing mic...
Article
Full-text available
The molecular mechanisms underlying atrial fibrillation (AF), the most common form of arrhythmia, are poorly understood and therefore target-specific treatment options remain an unmet clinical need. Excitation–contraction coupling in cardiac myocytes requires high amounts of adenosine triphosphate (ATP), which is replenished by oxidative phosphoryl...
Article
Full-text available
Aims: Atrial fibrillation is a commonly occurring arrhythmia after cardiac surgery (postoperative AF, poAF) and is associated with poorer outcomes. Considering that reduced atrial contractile function is a predictor of poAF and that Ca2+ plays an important role in both excitation-contraction coupling and atrial arrhythmogenesis, this study aims to...
Article
Full-text available
Aims: Atrial fibrillation is a commonly occurring arrhythmia after cardiac surgery (postoperative AF, poAF) and is associated with poorer outcomes. Considering that reduced atrial contractile function is a predictor of poAF and that Ca 2+ plays an important role in both excitation-contraction coupling and atrial arrhythmogenesis, this study aims to...
Article
To support scientific exchange and activity in the field of cardiac cellular electrophysiology, the German Cardiac Society Working Group on Cellular Electrophysiology (AG 18) established a two-day symposium to be held every 2 years. The second Ion Channel Symposium entitled “Göttingen Channels 2017—Of Benches and Beds” took place in Göttingen from...
Article
Full-text available
Rationale: Phosphodiesterase 2 (PDE2) is a dual substrate esterase, which has the unique property to be stimulated by cGMP, but primarily hydrolyses cAMP. Myocardial PDE2 is upregulated in human heart failure (HF), but its role in the heart is unknown. Objective: To explore the role of PDE2 in cardiac function, propensity to arrhythmia and in my...
Article
The purpose of this article is to review the basis of arrhythmogenesis, the functional and clinical role of the late Na current, and its therapeutic inhibition. Under pathological conditions such as ischemia and heart failure this current is abnormally enhanced and influences cellular electrophysiology as a proarrhythmic substrate in myocardial pat...
Article
Introduction: Pharmacological rhythm control of atrial fibrillation (AF) in patients with structural heart disease is limited. Ranolazine in combination with low dose dronedarone remarkably reduced AF-burden in the phase II HARMONY trial. We thus aimed to investigate the possible mechanisms underlying these results. Methods and results: Patch cl...
Article
Full-text available
Phosphodiesterase 2 (PDE2) is a dual substrate enzyme, hydrolyzing both cAMP and cGMP. We showed that myocardial PDE2 is upregulated in human and experimental heart failure (HF) while PDE3 and PDE4 are reduced. To explore the pathophysiological consequences of enhanced PDE2 activity, transgenic mice with a heart-specific overexpression of the PDE2A...
Article
Full-text available
Enhanced cardiac late Na-current (late INa) and increased sarcoplasmic reticulum (SR) Ca(2+)-leak are both highly arrhythmogenic. This study seeks to identify signalling pathways interconnecting late INa and SR-Ca(2+)-leak in atrial cardiomyocytes (CMs). In murine atrial CMs SR-Ca(2+)-leak was increased by the late INa enhancer ATX-II. An inhibitio...
Article
Contractile dysfunction and arrhythmogenic activity can arise if there are perturbations to calcium (Ca) homeostasis in the heart, such as in response to cardiotoxic substances. This investigation focuses on changes in cellular Ca homeostasis in response to the acute application of the cardiotoxic drug Clozapine (an atypical anti-psychotic).Rat ven...
Article
Chemical tissue fixation, followed by embedding in either agarose or Fomblin, is common practice in time-intensive MRI studies of ex vivo biological samples, and is required to prevent tissue autolysis and sample motion. However, the combined effect of fixation and sample embedding may alter tissue structure and MRI properties. We investigated the...
Article
Full-text available
This paper presents methods to build histo-anatomically detailed individualized cardiac models. The models are based on high-resolution three-dimensional anatomical and/or diffusion tensor magnetic resonance images, combined with serial histological sectioning data, and are used to investigate individualized cardiac function. The current state of t...
Article
We investigate acute effects of axial stretch, applied by carbon fibers (CFs), on diastolic Ca2+ spark rate in rat isolated cardiomyocytes. CFs were attached either to both cell ends (to maximize the stretched region), or to the center and one end of the cell (to compare responses in stretched and nonstretched half-cells). Sarcomere length was incr...

Questions

Question (1)
Question
I only have experience with Axoclamp 2B from when I was a student but now I have to use Axopatch 200B for perforated patch and there isn't much in the manual about perforated patch. My question is about "setting the cell up" before a perforated patch experiment, e.g. capacitance compensation, optimising the voltage clamp. When I used the Axoclamp 2B I used discontinuous single electrode voltage clamp (dSEVC) mode and an oscilloscope to monitor the switch clamp. How does this process differ with the Axopatch 200B? 

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