Flavio Carrion

Flavio Carrion
Universidad del Desarrollo | UDD · Clínica Alemana – UDD School of Medicine

PhD

About

79
Publications
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1,259
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Publications

Publications (79)
Article
Ovarian Cancer (OC) is the most common gynaecological malignancy and the eighth most diagnosed cancer in females worldwide. Currently, it is the fifth leading cause of cancer-related mortality among patients globally, largely due to delayed diagnosis, chemotherapy resistance, high metastasis rates, and subtype heterogeneity. Recent OC research high...
Article
Full-text available
COVID-19 continues to affect an unprecedented number of people with the emergence of new variants posing a serious challenge to global health. There is an expansion of knowledge in understanding the pathogenesis of Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and the impact of the acut...
Article
Full-text available
COVID-19 continues to affect an unprecedented number of people with the emergence of new variants posing a serious challenge to global health. There is an expansion of knowledge in understanding the pathogenesis of Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and the impact of the acut...
Article
Full-text available
Extracellular vesicles (EVs) are critical mediators of cell communication, playing important roles in regulating molecular cross-talk between different metabolic tissues and influencing insulin sensitivity in both healthy and gestational diabetes mellitus (GDM) pregnancies. The ability of EVs to transfer molecular cargo between cells imbues them wi...
Article
Objectives: To implement a dentin slice model of mesenchymal stem cells derived from dental tissues in a fibrin-agarose construct for dental pulp regeneration. Material and Methods: MSCs derived from different oral cavity tissues were combined with a fibrin-agarose construct at standard culture conditions. Cell viability and proliferation tests wer...
Article
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In recent years, conditioned medium (CM) obtained from the culture of mesenchymal stromal/stem cells (MSCs) has been shown to effectively promote tissue repair and modulate the immune response in vitro and in different animal models, with potential for application in regenerative medicine. Using CM offers multiple advantages over the implantation o...
Article
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Mesenchymal stem cells (MSC) have emerged as a promising tool to treat inflammatory diseases, such as inflammatory bowel disease (IBD), due to their immunoregulatory properties. Frequently, IBD is modeled in mice by using dextran sulfate sodium (DSS)-induced colitis. Recently, the modulation of autophagy in MSC has been suggested as a novel strateg...
Article
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Oxidative stress produces macromolecules dysfunction and cellular damage. Renal ischemia-reperfusion injury (IRI) induces oxidative stress, inflammation, epithelium and endothelium damage, and cessation of renal function. The IRI is an inevitable process during kidney transplantation. Preliminary studies suggest that aminoguanidine (AG) is an antio...
Article
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Mesenchymal stem cells (MSC) are adult multi-potent cells that can be isolated from many types of tissues including adipose tissue, bone marrow, and umbilical cord. They show great potential for cell therapy-based treatments, which is why they are being used in numerous clinical trials for a wide range of diseases. However, the success of placebo-c...
Article
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Mesenchymal stem cells (MSCs) have been recognized for their regenerative and anti-inflammatory capacity which makes them very attractive to cell therapy, especially those ones to treat inflammatory and autoimmune disease. Two different immune-phenotypes have been described for MSCs depending on which Toll-like receptor (TLR) is activated. MSC1 is...
Article
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Background: In type I diabetes mellitus (T1DM) pancreatic β cells are destroyed. Treatment entails exogenous insulin administration and strict diet control, yet optimal glycemic control is hardly attainable. Islet transplant could be an alternative in patients with poor glycemic control, but inefficient islet purification and autoimmune response o...
Article
Full-text available
Introduction: Dental pulp stem cells (DPSC) are very attractive in regenerative medicine. In this study, we focused on the characterization of the functional properties of mesenchymal stem cells derived from DPSCs. Currently, it is unknown whether inflammatory conditions present in an inflamed dental pulp tissue could alter the immunomodulatory pr...
Preprint
Full-text available
Background. In type I diabetes mellitus (T1DM) pancreatic β cells are destroyed. Treatment entails exogenous insulin administration and strict diet control, yet optimal glycemic control is hardly attainable. Islet transplant could be an alternative in patients with poor glycemic control, but inefficient islet purification and autoimmune response of...
Preprint
Full-text available
Background: In type I diabetes mellitus (T1DM) pancreatic β cells are destroyed. Treatment entails exogenous insulin administration and strict diet control, yet optimal glycemic control is hardly attainable. Islet transplant could be an alternative in patients with poor glycemic control, but inefficient islet purification and autoimmune response of...
Preprint
Full-text available
Background: In type I diabetes mellitus (T1DM) pancreatic β cells are destroyed. Treatment entails exogenous insulin administration and strict diet control, yet optimal glycemic control is hardly attainable. Islet transplant could be an alternative in patients with poor glycemic control, but inefficient islet purification and autoimmune response of...
Article
Full-text available
Group B Streptococcus (GBS) is the primary etiological agent of sepsis and meningitis in newborns and is associated with premature birth and stillbirth. The development of a licensed vaccine is one of the pending challenges for the World Health Organization. Previously, we showed that oral immunization with surface immune protein (SIP) decreases va...
Article
Full-text available
Vaccine-induced protection against pathogens, especially subunit-based vaccines, are related to antigen properties but mainly in their ability to stimulate the immune system by the use of an adjuvant. Modern vaccines are formulated with a high level of antigen purity, where an efficient adjuvant is necessary. In this context, the use of protein Tol...
Article
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Background: Epanorin (EP) is a secondary metabolite of the Acarospora lichenic species. EP has been found in lichenic extracts with antimicrobial activity, and UV-absorption properties have been described for closely related molecules; however, its antiproliferative activity in cancer cells has not yet been explored. It has been hypothesized that...
Article
Group B Streptococcus (GBS) represents one of the most common causes of bacterial infection in neonates; it is also associated with premature childbirth and stillbirth. A vaccine against GBS is needed, but no approved vaccines are yet available. The Surface Immunogenic Protein (SIP) of GBS is conserved in all serotypes and had been reported to be a...
Preprint
Full-text available
We previously described the protective role of NFAT5 during hypoxia, in an independent way of HIF-1α. Alternatively, inducible NO synthase (iNOS) is also induced by hypoxia. The aim of this study was to establish the NFAT5 target gene in mouse embryonic fibroblasts (MEF) cell stimulated by hypoxia. NFAT5, iNOS, NO level, aquaporin 1 (AQP1) and urea...
Article
Full-text available
The therapeutic effect of mesenchymal stem cells (MSCs) in multiple sclerosis (MS) and the experimental autoimmune encephalomyelitis (EAE) model has been well described. This effect is, in part, mediated through the inhibition of IL17-producing cells and the generation of regulatory T cells. While proinflammatory cytokines such as IFNγ, TNFα, and I...
Data
IL17 mesenchymal stem cells (MSCs) pretreatment increases MSCs therapeutic potential in experimental autoimmune encephalomyelitis (EAE). (A) Daily evaluation of clinical score was performed from the day of MSCs administration until euthanasia. (B) The area under the curve (AUC) of the clinical score for each treatment was calculated and compared. L...
Data
Phenotypic characterization and differentiation potential of wild-type (WT) and L17RA−/− mesenchymal stem cells (MSCs). (A) WT and IL17RA−/− MSCs were stained with specific antibodies against CD45, CD34, CD29, CD44, and Sca-1 and analyzed by flow cytometry. Histograms are representative of at least three independent experiments. (B) Differentiation...
Poster
Full-text available
GBS is the main infectious agent associated to neonatal sepsis, which becomes infected when it passes through birth canal. Treatments with antibiotics before they are produce the birth are indicated to avoid this infection. Vaccines are in development and different treatments based on probiotics have been reported. The Biotechnology Section of the...
Article
Full-text available
Background Recently, it has been observed that mesenchymal stem cells (MSCs) can modulate their immunoregulatory properties depending on the specific in-vitro activation of different Toll-like receptors (TLR), such as TLR3 and TLR4. In the present study, we evaluated the effect of polyinosinic:polycytidylic acid (poly(I:C)) and lipopolysaccharide (...
Article
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The neurotransmitter GABA has been recently identified as a potent immunosuppressive agent that targets both innate and adaptive immune systems and prevents disease progression of several autoimmunity models. Mesenchymal stem cells (MSCs) are self-renewing progenitor cells that differentiate into various cell types under specific conditions, includ...
Article
Background aims: Immunomodulatory properties of human umbilical cord-derived mesenchymal stromal cells (UCMSCs) can be differentially modulated by toll-like receptors (TLR) agonists. Here, the therapeutic efficacy of short TLR3 and TLR4 pre-conditioning of UCMSCs was evaluated in a dextran sulfate sodium (DSS)-induced colitis in mice. The novelty...
Article
Full-text available
Background: Intracoronary delivery of autologous bone marrow mononuclear cells is an interesting therapeutic promise for patients with heart failure of different etiologies. Aim: To evaluate the long-term safety and efficacy of this therapy in patients with dilated cardiomyopathy of different etiologies under optimal medical treatment. Patients...
Data
Full-text available
Supplementary figure 1: Correlation of Th1, Th17 and Treg analysis by flow cytometry and RT-qPCR.
Article
Mesenchymal stem cells (MSCs) can be isolated from dental tissues, such as pulp and periodontal ligament; the dental apical papilla (DAP) is a less-studied MSC source. These dental-derived MSCs are of great interest because of their potential as an accessible source for cell-based therapies and tissue-engineering (TE) approaches. Much of the intere...
Article
Periodontal disease is one of the most common conditions affecting humans, and current treatment strategies, which focus on the removal and long-term control of dental plaque, are generally successful in eliminating active disease and promoting tissue repair. However, regeneration of the supporting structures of the tooth remains an elusive goal an...
Article
Full-text available
The Silences of the Archives, the Reknown of the Story. The Martin Guerre affair has been told many times since Jean de Coras and Guillaume Lesueur published their stories in 1561. It is in many ways a perfect intrigue with uncanny resemblance, persuasive deception and a surprizing end when the two Martin stood face to face, memory to memory, befor...
Article
Full-text available
Background: Intracoronary delivery of autologous bone marrow mononuclear cells is an interesting therapeutic promise for patients with heart failure of different etiologies. Aim: To evaluate the long-term safety and efficacy of this therapy in patients with dilated cardiomyopathy of different etiologies under optimal medical treatment. Patients and...
Article
Full-text available
Available medical therapy is unable to completely prevent or revert the pathological cardiac remodeling secondary to ischemia or other injuries, which is responsible for the development of heart failure. Regenerative medicine through stem cells had an explosive development in the cardiovascular area during the past decade. Stem cells possess the ca...
Article
Background and Aim: Mesenchymal stem cells (MSCs) have attracted much attention since exert anti-inflammatory and immunomodulatory effects. Recently, diverse studies have been showed that the stimulation of specific toll-like receptors affects the immunomodulatory properties of MSCs. The aim of this work was to evaluate the effects of TLR precondit...
Article
Full-text available
It was recently discovered that dental tissues are important sources of mesenchymal stem cells (MSCs). In the oral cavity MSCs can be found in the pulp, dental follicle, apical papilla and gingival tissue, among others. Many studies support the therapeutic potential of MSCs in regenerative therapies. The objective of this study was to isolate and c...
Article
Full-text available
The aim of this study was to assess and describe the morphological effects of an intra-articular iniection of Mesenchymal Stem Cells (MSCs) and/or Low Intensity Pulsed Ultrasound (LIPUS) stimulation on the mandibular condyles of growing rats, using cone beam computed tomography (CBCT) and histology. Twenty-six young (23-day-old) rats were divided i...
Article
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Background: Recent studies indicate that intra-coronary delivery of autologous bone marrow mono-nuclear cells (BMCs) improves the ejection fraction (LVEF) and other clinical markers in patients with heart failure (HF). Aim: To evaluate the safety and efficacy of intraco-ronary delivery of autologous BMCs in patients with HF in dilated phase under o...
Conference Paper
Objective: To assess biocompatibility of an hydrogel scaffold (BD-Matrigel) with MSCg in a tooth model in vitro. Method: Mesenchymal stem cells were isolated from gingival connective tissue, immunophenotype was characterized by flow citometry, using specific antibodies markers. MSCg were differentiated into adipocytes, osteoblasts and chondroblas...
Article
The Chilean population is ethnically diverse, and more than 50% of children referred for hematopoietic stem cell transplantation (HSCT) lack a suitable donor. To expand the donor pool, we assessed the feasibility, tolerance, and efficacy of using a haploidentical (HI) donor and a reduced-intensity conditioning regimen for high-risk pediatric leukem...
Article
Full-text available
Th17 cells, a recently described subtype of CD4+ effector lymphocytes, have been linked to cell-mediated autoimmune and inflammatory diseases as well as to cardiovascular diseases. However, the participation of IL-17A in myocardial ischemic injury has not been clearly defined. We therefore conducted the present study to evaluate IL-17A and Th17-rel...
Article
Aim(s): To explore the relationship between biomarkers of systemic inflammation in plasma and gingival crevicular fluid in early pregnancy and the subsequent development of pre-eclampsia in patients with periodontitis. Materials and methods: A case-control study was performed. From a cohort composed of 126 pregnant women, 43 normotensive healthy...
Article
Full-text available
MSC display potent suppressive properties initially described a decade ago. More recently, MSC suppressive activities on T-cell effector pathways have been investigated. MSC modulate CD4 differentiation through different mechanisms depending on culture conditions and display disparate activities on T cells according to their differentiation status....
Conference Paper
Objective: Lineage Th17 has been involved in inflammatory and autoimmune disease. Periodontitis is an infectious and inflammatory disease that involves a host immune response and is characterized by alveolar bone destruction. Aim is the study of the involvement of TH17 axis in the severity of periodontal disease in Gingival Crevicular Fluid (GCF) a...
Article
Full-text available
Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawle...
Article
Mesenchymal Stem Cells (MSCs) can be affected in their capabilities to proliferate in vitro under physical and/or biochemical stimulation. The aim of this study was to select an optimal intensity level for low intensity pulsed ultrasound (LIPUS) stimulation of Sprague-Dawley bone marrow MSCs proliferation in vitro. Bone marrow MSCs of Sprague-Dawle...
Article
Full-text available
Mesenchymal stem cells (MSCs) are now known to display not only stem cell multipotency, but also robust antiinflammatory and regenerative properties. After widespread in-vitro and in-vivo preclinical testing, autologous and allogeneic MSCs have been applied in a range of immune mediated conditions, including graft versus host disease, Crohn's disea...
Article
Full-text available
Mesenchymal stem cells (MSCs) are now known to display not only stem cell multipotency, but also robust antiinflammatory and regenerative properties. After widespread in-vitro and in-vivo preclinical testing, autologous and allogeneic MSCs have been applied in a range of immune mediated conditions, including graft versus host disease, Crohn's disea...
Article
Full-text available
Aim: The purpose of this study was to assess the proliferation of mesenchymal stem cells (MSCs) from human gingival tissue on chitosan matrix. Methods: Experimental study in vitro. Gingival connective tissue samples were obtained from healthy volunteers from the maxillary tuberosity. The explants were minced and cultured on tissue culture dishes. M...
Article
4581 The Chilean population is ethnically diverse, and more than 50% of the children referred for hematopoietic stem cell transplantation lack a suitable donor. To expand the donor pool we assessed the feasibility, tolerance, and efficacy of using a parental haploidentical (HI) donor and a reduced-intensity conditioning regimen for patients with hi...
Conference Paper
Objective: To establish the effects of Mesenchymal Stem Cell (MSCs) transplantation in the TMJ region of Sprague-Dawley rats treated with Low Intensity Pulsed Ultrasound (LIPUS) using Cone Beam Computed Tomography (CBCT) 3D reconstructions. Methods: 26 male rats (20 days) were divided into 5 groups: A, LIPUS-stimulated; B, MSCs injection, C; LIPUS+...
Conference Paper
Objectives: Scientific evidence suggests an association between periodontal diseases and adverse pregnancy outcome, the aim of this prospective study was to investigate the relationship between periodontal clinical changes, progression and severity of gingivitis and Periodontitis. Methods: This is a prospective study of one population cohort that w...
Article
DiGeorge syndrome presents dysmorphic facial structures, conotruncal cardiac defects, parathyroid, thymus, airway malformations and a broad range of T-cell deficiency. Objetives. To describe the clinical findings, frequency and type of airway malformations and CD3, CD4 and CD8 T-cells counts. Patients and methods. A multicentric, retrospective char...
Article
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Background: Hyper-IgM syndrome (HIGM) is a rare primary immunodeficiency used to describe a heterogeneous group of disorders characterized by recurrent bacterial infections, normal or elevated serum IgM levels and low or absent serum IgG, IgA and IgE. Aim: To make definitive diagnosis, detect mutations in carriers and perform genetic counseling in...
Article
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DiGeorge syndrome is characterized by developmental defects of the heart, parathyroid glands and thymus. Aim: To describe the clinical variability of DiGeorge syndrome and its relation with immunodeficiency. Patients and methods: A three years retrospective chart review from three hospitals of Santiago, Chile was conducted. We included patients wit...
Article
Acute rheumatic fever (ARF) and its chronic valvular sequelae are the delayed consequence of a pharyngeal infection with group A Streptococcus (GAS). Several GAS proteins have been shown to be superantigens, raising the possibility that the expansion or deletion of T cells expressing specific Vbeta regions might play a role in the pathogenesis of A...
Article
Rheumatic Heart Disease (RHD) is a delayed consequence of a pharyngeal infection with group A streptococcus (GAS), usually ascribed to a cross-reactive immune response to the host's cardiac tissues. Several GAS proteins have been reported to be superantigens, also raising the possibility that T cells in RHD could be driven by superantigens. We ther...
Article
Background: Polymorphisms of Fc receptors for IgG (FcgR) have been proposed as a genetic factor that influences susceptibility for systemic lupus erythematosus (SLE). Human FcgRIIa has 2 codominantly expressed alleles, H131 and R131, which differ at amino acid position 131 in the second extracelular domain (histidine or arginine respectively) and d...
Article
Full-text available
To determine the frequency of occurrence and long term evolution of subclinical carditis in patients with acute rheumatic fever. Valvar incompetence was detected by clinical examination and Doppler echocardiographic imaging during the acute and quiescent phases of rheumatic fever. Patients were followed prospectively and submitted to repeat examina...
Article
During the last few decades, basic scientists and clinicians have gained a deeper insight of the cellular and molecular physiology of the immune system. The widespread application of molecular biology and genetic techniques has advanced our understanding of states of health and disease, bringing forth renewed hopes concerning the advent of a more "...
Article
To determine if activation-induced cytokine production is altered in CD2+ lymphocytes from B-CLL patients, cytokine levels were determined by ELISA in supernatants of PHA-stimulated cultures of CD2+ cells from 33 B-CLL patients and 22 healthy controls. The production of Interferon gamma (IFN-gamma) and Tumor Necrosis Factor (TNF-alpha) by mitogen-a...
Article
Neuroendocrine factors play an important role in the expression of autoimmune diseases. Prolactin (PRL) can induce T-cell proliferation and macrophage activation. Elevated PRL levels have been described in patients with rheumatoid arthritis (RA). We studied immunological and clinical effects of PRL suppression in 9 RA patients with active disease,...