Fiorenzo Marinelli

• Phd
• Italian National Research Council

Exposure to artificial radio frequency electromagnetic fields (RF-EMFs) has greatly increased in recent years, thus promoting a growing scientific and social interest in deepening the biological impact of EMFs on living organisms. The current legislation governing the exposure to RF-EMFs is based exclusively on their thermal effects, without consid...

As part of the general assessment of the electromagnetic pollution present in a given area, campaigns of measurements of atmospheric electrical parameters have been carried out for a long time with an air ion meter, designed and built by us through a difficult path: "Ionmetro" ". Each of these campaigns was born from requests from Municipalities or...

Background and aim of the work: This study investigates the possible association between the prevalence of some chronic and lethal diseases in the population and the exposure to the EMF radiation of the military radar ARGOS 10, that had been located since 1970 until 1998 in the hamlet of Casette Antonelli, Potenza Picena (MC), Italy. Methods: Five...

Enzymatic activity and conformational features of bovine lactoperoxidase (LPO), ascorbate oxidase (AO) and laccase (LAC) are modified when acqueus solutions of these enzymes are exposed to the microwave fields emitted by a mobile phone. Electromagnetic fields (EMF) interfere with Free Radicals intermediate species of the electron transfer enzymatic...

PRESENTAZIONE Durante le vacanze natalizie del 2011 ho avuto la fortuna di conoscere il prof. Massimo Scalia, un fisico-matematico docente della Facoltà di Scienze Matematiche, Fisiche e Naturali dell’Università di Roma “La Sapienza”. Scalia è, insieme al prof. Gianni Mattioli, uno dei padri dell’ambientalismo scientifico italiano; leader delle ba...

This work assesses the environmental impact from chemical emissions due to military tests and routine activities in the area occupied by the Italian Inter-force Test Range (PISQ), located at Salto di Quirra, Sardinia, Italy. After reviewing the military activities carried out at PISQ, such as rocket launching, blasting and armament destruction, pro...

The original article to which this Erratum was published in J. Cell. Physiol. 198:324–332, 2004 It has been recently established that low-frequency electromagnetic field (EMFs) exposure induces biological changes and could be associated with increased incidence of cancer, while the issue remains unresolved as to whether high-frequency EMFs can have...

The structure of chromatin is strictly related to its function. During the apoptosis, induced by APO-1/FAS treatment, it is possible to evidentiate, by electron microscopy, different organisation steps of the chromatin ultrastructure. By using fractal and multifractal analysis it is possible to identify a progressive change of chromatin arrangement...

It has been recently reported that retinoblastoma family proteins suppress cell growth by regulating not only E2F-dependent mRNA transcription but also rRNA and tRNA transcription and, through HDAC1 recruitment, chromatin packaging. In the present study we report data showing that these various control strategies are correlated, at least in part, w...

Biological effects of extremely low frequency (ELF) electromagnetic fields (EMF) seem to be mediated by the plasma membrane. Many hypotheses have been suggested to explain the mechanisms by which ELFEMF affect cell functions. In particular it has been proposed that EMF may affect the cell receptor function, or ion channel formation or receptor capp...

In this study we analyse the chromatin contour in electron microscopic images of human peripheral lymphocytes by fractal dimension (FD), in order to demonstrate the different FD values in different nuclear domains. Fractal analysis shows that the heterochromatin associated with the nuclear envelope (NEHC) has an FD value of 1.248, lower than the ot...

To evaluate the bone-pin interface in hydroxyapatite coated versus uncoated pins. Eighty-four bicylindrical stainless steel external fixation pins were implanted in a test group of 14 sheep. One-half of the pins were coated with hydroxyapatite and the rest remained uncoated. Six coated pins were implanted in the left tibia of seven sheep, and six u...

Intramembrane proteins (IMP) represent a class of proteins located in the lipid bilayer of the cell membrane which function as ion channels, enzymes or receptors. Since it has been argued that biological effects of extremely low frequency (ELF) electromagnetic fields are mediated by plasma membrane. this work was designed to study the possible effe...

The rat pheochromocytoma PC12 cell line, which differentiates into sympathetic neurons under nerve growth factor (NGF) treatment, contains at least three phosphoinositidase C (PIC) isozymes, PIC beta, PIC gamma, PIC delta. These isozymes have been previously shown to display a different subcellular localization. To determine whether or not NGF indu...

In this study the effect of pulsed magnetic fields (PMF) on the distribution of the intramembrane proteins in Swiss 3T3 cell was investigated by freeze-fracture technique and image statistical analysis. The results shows a significant degree of clusterization due to the PMF exposure.

The application of image analysis methods to conventional thin sections for electron microscopy to analyze the chromatin arrangement are quite limited. We developed a method which utilizes freeze-fractured samples; the results indicate that the method is suitable for identifying the changes in the chromatin arrangement which occur in physiological,...

The complex organization of the interphase nucleus can be analyzed, by way of thin sectioning and also freeze-fracture. This approach has previously been utilized in association with image analysis to quantitatively describe the organization of isolated rat liver nuclei and nuclear matrices. The main nuclear domains which, in section, present marke...

Most methods which are used to prevent a specific colloidal gold (c.g.) binding in cytochemical reactions are based on the addition of macromolecules to the incubation medium. A new method based on the effects of glow discharge (GD) plasma is reported.

Computerized image analysis was performed on freeze-fractured nuclei isolated from normal rat liver and rat liver with diethylnitrosamine induced hepatocarcinomas. Chromatin particles belonging to heterochromatin and interchromatin nuclear regions were measured, distributed into histograms and statistically processed. The ratio between nucleosome f...

We characterized the mucus glycoconjugates produced by goblet and non-goblet epithelial cells in asymptomatic contact lens (CL) wearers. We employed four lectins (proteins of vegetal origin which specifically recognize glycosidic residues: WGA, PNA, SBA and ConA) conjugated with colloidal gold as ultrastructural marker, at Transmission Electron Mic...

The variation sources relevant to a diagnostic morphometric study were analysed. The influence of each source was estimated in two experiments, performed in systems applying computer assisted interactive morphometry. In the first experiment one observer measured the areas of a large number of nuclei in a section from a grade II transitional cell ca...

Ethidium bromide (EB) is widely used for investigating the DNA conformation in chromatin both with conventional and cytofluorimetric techniques. Since the interaction of the dye with DNA should result in structural deformations which can be different in isolated or in situ chromatin, a study has been performed on the effects caused by different amo...

Isolated nuclei represent a suitable model for studying the influence of exogenous phospholipids, normally found as minor chromatin components, on the nuclear structure, which, in turn, could be related to the observed modifications of DNA and RNA synthesis. The morphological modifications induced on chromatin RNP granules and nuclear matrix have b...

Interphase rat liver nuclei were studied by freeze fracturing followed by electron microscopic observations. This method permits information on the native organization of the nuclear components in the hydrated state to be obtained. Morphometric analyses, performed with a Leitz Texture Analysis System, gave precise information on the different nucle...

Benign and malignant mesothelial lesions of the pleura are quantitatively studied using a Leitz TAS image analyzing system. The Feulgen fast green staining method allows performing on the same cell measurements of the shape and size of the nucleus, the size of the nucleolus and the content of DNA. Numerical data are studied in a multivariate statis...

The validity of a test system in morphometric histopathology depends on the variation sources involved. Biological variation is one of the variation sources, but is also the object to be studied with morphometry. We studied the variation sources in interactive computerized morphometry in two test systems involving two different commercially availab...

The morphology of intact or membrane-deprived interphase nuclei has been analysed by freeze-fracture electron microscopy. This method appears particularly useful for providing information on the distribution and organisation of chromatin and ribonucleoproteins in the absence of dehydration and embedding artifacts of conventional electron microscope...

Nuclear matrices, purified from normal and chronic lymphocytic leukemia lymphocytes, exhibit a close association with the centriole. This finding suggests that the nuclear and cytoplasmic skeletal systems are linked by transmembrane connections represented by nuclear matrix constituents. This could account for the observed synchrony between transfo...