Filomena Silva

Filomena Silva
ARAID · Faculty of Veterinary Science University of Zaragoza

PhD

About

44
Publications
23,416
Reads
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2,014
Citations
Citations since 2017
13 Research Items
1524 Citations
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20172018201920202021202220230100200300
20172018201920202021202220230100200300
20172018201920202021202220230100200300
Introduction
My PhD research focused on optimizing plasmid DNA production fermentation processes using Escherichia coli. This research was a part of an R&D project whose purpose was the creation of a pilot plant for the production of DNA vaccines. In my post-doc, I'm expanding my research to the field of Food Science, pursuing an investigation more related to antimicrobial food packaging and functional foods with antimicrobial properties.
Additional affiliations
January 2008 - December 2010
Universidade da Beira Interior
Position
  • Plasmid DNA production, recovery and purification
Position
  • Laboratory classes of Cellular and Molecular Biology and Microbiology
Education
January 2008 - December 2011
October 2002 - August 2007

Publications

Publications (44)
Article
Full-text available
The increasing resistance of pathogenic microorganisms against common treatments requires innovative concepts to prevent infection and avoid long-term microbe viability on commonly used surfaces. Here, we report the preparation of a hybrid antimicrobial material based on the combination of microbiocidal polyoxometalate-ionic liquids (POM-ILs) and a...
Article
Full-text available
PurposeAiming to exploit the remediation of olive cake and leaves, a comparative study between these extracts from the same trees has been carried out to explore them as a source of bioactive compounds with added value.Methods Olive cake (OCE) and leaf (OLE) methanolic extracts were chemically characterized by UPLC-QTOF-MS, and tested for their in...
Article
Background Nowadays, consumers are more aware about what they eat and how it is packaged. Food quality is of paramount importance and consumers are eager to be constantly updated about the perishability and shelf-life of the food products they buy. These needs gave rise to intelligent packaging (IP) materials and devices that can communicate with a...
Article
Two essential oils and seven vegetable oils were tested in an in situ generator of hydroxyl radicals for determining their antioxidant capacity. Then twenty-two different active LDPE films were prepared by incorporating both the essential oils and vegetable oils. The antioxidant capacity, heat-sealing properties and aroma of developed films were de...
Article
Full-text available
Antimicrobially active packaging has emerged as an effective technology to reduce microbial growth in food products increasing both their shelf-life and microbial safety for the consumer while maintaining their quality and sensorial properties. In the last years, a great effort has been made to develop more efficient, long-lasting and eco-friendly...
Chapter
The seeds and leaves of coriander plant are commonly used spices around the world. Since antiquity, coriander has been used in folk medicine, and more recently, parts of the plant, extracts/oils, and other pure compounds extracted from the plant have started to be used in the cosmetic and food industries as aromas and flavoring substances. Coriande...
Article
Full-text available
Food packaging is tailored to keep food fresh by increasing shelf life and preventing microbial deterioration. However, traditional food packaging is commonly made from non-degradable polymers without antimicrobial properties and that pose an environmental threat if not disposed properly. To address this issue, here we describe the preparation of c...
Article
Microorganisms such as bacteria, fungi, algae and moulds are highly proficient at colonizing artistic and architectural heritage. The irreparable damage they cause to unique artefacts results in immeasurable cultural and societal losses to our shared cultural heritage, which represent an important social and economic resource for Europe. With the o...
Article
In this work, we exploit the antimicrobial properties of coriander essential oil (CEO) incorporated in dextrin-derived nanosponges to create a stable controlled release system. Nanosponges (CD-NS) were loaded with CEO, characterized by means of scanning and transmission electron microscopy (SEM and TEM) and X-ray diffraction (XRD), and tested for t...
Article
The main goal of this work is the encapsulation of cinnamon essential oil in cyclodextrin nanosponges and the assessment of their antimicrobial activity against foodborne pathogens. After nanosponge synthesis, a headspace-solid phase microextraction coupled to gas chromatography-mass spectrometry (HS-SPME-GC-MS) method was validated to quantify ess...
Article
Fresh chicken is a potential source of human infection due to its high microbial load, making the issues regarding its microbiological safety, especially the control of Campylobacter spp., of paramount relevance. Pinosylvin has recently proved effective against this major food pathogen. In this work, we evaluated the efficacy of absorbent pads cont...
Chapter
The use of ethyl lauroyl arginate (LAE) as an antimicrobial and food preservative is currently approved by the Food and Drug Administration (FDA) and the European Food Safety Agency (EFSA), respectively. Due to this fact, in recent years, there has been a great focus on the possible application of this compound to food systems. In this chapter, alt...
Chapter
Active packaging has emerged as a reliable alternative for food preservation by introducing protective agents in the packaging materials, replacing the direct addition of new chemicals into the food product. This chapter will describe not only the migration process concerning the transfer of the active agent from the material toward the product, bu...
Article
Fresh poultry meat and poultry products are highly perishable foods and high potential sources of human infection due to the presence of several foodborne pathogens. Focusing on the microbial control of poultry products, the food industry generally implements numerous preventive measures based on the HACCP food safety management system certificatio...
Article
In this work, the effects of cinnamon and mustard essential oils against nine foodborne bacteria were evaluated. Their antimicrobial susceptibility and action mechanism was also assessed. Additionally, the effects of the combination between both were evaluated. To provide data on the in vitro action of essential oils, the transfer mechanism was ass...
Article
Worldwide, the consumption or handling of contaminated food has been described as one of the major causes of foodborne illness with campylobacteriosis being the most commonly reported zoonosis. Campylobacter jejuni and Campylobacter coli are considered the major cause of bacterial gastroenteritis, while Arcobacter spp. are also known to be human an...
Article
Full-text available
Foodborne illness represents a major economic burden worldwide and a serious public health threat, with around 48 million people affected and 3000 death each year only in the USA. One of possible strategies to reduce foodborne infections is the development of effective preservation strategies capable of eradicating microbial contamination of foods....
Article
Due to the highly epidemic character of Campylobacter infections and to the increased antibiotic resistance of these microorganisms, new approaches to control them are highly demanded. In this work, we investigated the anti-Campylobacter activity and mode of action of stilbenes encapsulated in cyclodextrins. Results showed that pterostilbene and pi...
Article
Full-text available
Resveratrol is a plant secondary metabolite commonly found in peanuts and grapevines with significant health benefits. Recombinant organisms can produce large amounts of resveratrol and, in this work, Escherichia coli BW27784 was used to produce resveratrol in bioreactors while monitoring cell physiology and plasmid stability through flow cytometry...
Article
Full-text available
Catechol-O-methyltransferase (COMT, EC 2.1.1.6) plays a crucial role in dopamine metabolism which has intimately linked this enzyme to some neurodegenerative diseases, such as Parkinson's disease. In recent years, in the attempt of developing new therapeutic strategies for Parkinson's disease, there has been a growing interest in the search for eff...
Article
Full-text available
The frequent isolation of Arcobacter butzleri and Arcobacter cryaerophilus from food samples makes it imperative to search for potential compounds able to inhibit the development of these bacteria. Taking this into consideration, this study focuses on the antimicrobial activity of resveratrol and its mechanism of action against A. butzleri and A. c...
Article
Staphylococcus aureus is a Gram-positive pathogen which is able to form biofilms, exhibiting a more pronounced resistance to antibiotics and disinfectants. The hurdles posed in eradicating biofilms have driven the search for new compounds able to fight these structures. Phenolic compounds constitute one of the most numerous and ubiquitous group of...
Conference Paper
Export Date: 28 November 2013, Source: Scopus, Article in Press, CODEN: JBITD, doi: 10.1016/j.jbiotec.2013.10.021, Language of Original Document: English, Correspondence Address: Gouveia, I.C.; R and D Unit of Textile and Paper Materials, Faculty of Engineering, University of Beira Interior, 6email: igouveia@ubi.pt
Article
In the context of recombinant DNA technology, the development of feasible and high-yielding plasmid DNA production processes has regained attention as more evidence for its efficacy as vectors for gene therapy and DNA vaccination arise. When producing plasmid DNA in Escherichia coli, a number of biological restraints, triggered by plasmid maintenan...
Article
In this study we investigated the existence of synergistic antibacterial effect between coriander (Coriandrum sativum L.) essential oil and six different antibacterial drugs (cefoperazone, chloramphenicol, ciprofloxacin, gentamicin, tetracycline and piperacillin). The antibacterial activity of coriander oil was assessed using microdilution suscepti...
Article
In this study we investigated the existence of synergistic antibacterial effect between coriander (Coriandrum sativum L.) essential oil and six different antibacterial drugs (cefoperazone, chloramphenicol, ciprofloxacin, gentamicin, tetracycline and piperacillin). The antibacterial activity of coriander oil was assessed using microdilution suscepti...
Article
In order to provide sufficient pharmaceutical-grade plasmid DNA material, it is essential to gain a comprehensive knowledge of the bioprocesses involved; so, the development of protocols and techniques that allow a fast monitoring of process performance is a valuable tool for bioprocess design. Regarding plasmid DNA production, the metabolic stress...
Article
Full-text available
The aim of this work was to study the antibacterial effect of coriander (Coriandrum sativum) essential oil against Gram-positive and Gram-negative bacteria. Antibacterial susceptibility was evaluated using classical microbiological techniques concomitantly with the use of flow cytometry for the evaluation of cellular physiology. Our results showed...
Article
The translation of non-viral gene replacement therapies for cancer into clinical application is currently hindered due to known issues associated with the effectiveness of plasmid DNA (pDNA) expression vectors and the production of gene delivery vehicles. Herein we report an integrative approach established on the synthesis of nanoparticulated carr...
Article
The increasing incidence of drug-resistant pathogens and toxicity of existing antifungal compounds has drawn attention towards the antimicrobial activity of natural products. The aim of the present study was to evaluate the antifungal activity of coriander essential oil according to classical bacteriological techniques, as well as with flow cytomet...
Article
Full-text available
As common microbiological methods for the assessment of bacteriostatic or bactericidal activities are very time-consuming, in this work we describe that the use of a novel far-red fluorescent stain, Vybrant DyeCycle Ruby (DCR) for the flow cytometric analysis of fluoroquinolone (ciprofloxacin) bacteriostatic and bactericidal activities in Escherich...
Article
DNA-based vaccines and gene therapies have rapidly evolved since the first demonstration of in vivo gene expression by naked DNA injection. When optimizing their production, the major goals in the fermentation process are to maximize the specific plasmid yield, as well as plasmid quality, while minimizing plasmid-mediated metabolic burden. Due to t...
Article
Full-text available
Flow cytometry provides a rapid and high-content multiparameter analysis of individual microorganisms within a population. In the past years, several fluorescent stains were developed in order to monitor DNA content distribution and cell-cycle phases, mainly in eukaryotic cells. Recently, due to its low detection limits, several of these fluorescen...
Article
Full-text available
The obtention of high yields of purified plasmid DNA is viewed as an essential issue to be considered towards efficient production of DNA vaccines and therapeutic plasmids. In this work, Escherichia coli DH5alpha bearing the pVAX1-LacZ plasmid was grown in a developed semi-defined medium at different temperatures and tryptone concentrations. Analys...

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