Filippe Elias de Freitas Soares

Filippe Elias de Freitas Soares
Universidade Federal de Lavras (UFLA) | UFLA · Departamento de Química

Ph.D. Biochemistry
Professor at Federal University of Lavras, Minas Gerais, Brazil

About

97
Publications
13,195
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762
Citations
Additional affiliations
May 2018 - April 2019
Universidade Federal de Goiás
Position
  • Visiting Professor
May 2017 - December 2017
Universidade do Estado de Minas Gerais
Position
  • Professor
June 2015 - April 2017
Universidade Federal de Viçosa (UFV)
Position
  • PostDoc Position

Publications

Publications (97)
Article
Full-text available
Knowledge of nematophagous fungi has increased dramatically over recent years, particularly with the advancement of molecular biology and omics sciences. However, most of this knowledge is restricted to the three traditional groups of nematophagous fungi: predatorial, opportunistic or ovicidal and endoparasitic. The present study supported the prop...
Article
Full-text available
Synadenium grantii is a Euphorbiaceae plant commonly found in Brazil, known as Janaúba or Leitosinha, whose latex is traditionally used for several purposes. However, it is not known whether the nematicidal action of this plant latex occurs due to the action of proteases. The present work aims to evaluate the nematicidal activity of proteases from...
Article
Spent mushroom compost (SMC) is a residue generated in edible mushrooms production, such as Hypsizygus marmoreus. Its genome was recently sequenced, demonstrating cuticle-degrading protease genes. The present work aims to investigate the proteases from H. marmoreus spent mushroom compost (SMC) by verifying its action on nematode larvae. The extract...
Article
Full-text available
Nutritional factors exert significant influence on the growth of entomopathogenic fungi, one of the main agents employed commercially in the biological control of arthropods. Thus, the objective of this work is to optimize the culture medium and solid fermentation time for production of proteases and conidia of Metarhizium robertsii ARSEF 2575 and...
Article
Cattle parasitic otitis caused by the nematode Rhabditis spp. is a serious health problem in Brazil, a situation which is confounded by lack of effective control measures. In vitro studies associating biological and chemical control as an alternative method showed promising results. The objective was to evaluate the combined use of Duddingtonia fla...
Article
Full-text available
In this review, we supply a framework for the importance of nematophagous fungi (nematophagous fungi [NF]) and their role in agricultural ecosystems.We characterize the taxonomy, diversity, ecology, and type of NF, depending on their interaction with plant- parasitic nematodes (PPNs). We described potential mechanisms of NF in the control of PPNs,...
Article
The mushroom Lentinula edodes, known as shiitake, has the potential to control plant diseases. However, there is no work that mentions the nematicidal activity of the SMC from shiitake. The aim of the present study is to evaluate the nematicidal potential of L. edodes spent mushroom compost (SMC) on Panagrellus spp. SMC action on Panagrellus spp. l...
Article
Full-text available
Climate change is causing detrimental changes in living organisms, including pathogens. This review aimed to determine how climate change has impacted livestock system management, and consequently, what factors influenced the gastrointestinal nematodes epidemiology in small ruminants under tropical conditions. The latter is orientated to find out t...
Article
Recently, the production process of a nanoparticle with nematicidal activity from a nematophagous fungus was developed. Thus, a new strategy to control parasites has been discovered. The aim of the present study was to evaluate the in vitro activity of silver nanoparticles (AgNP’s) biosynthesized from Duddingtonia flagrans filtrate on the control o...
Article
Rhipicephalus microplus (Canestrini 1888) causes severe economic losses to the cattle industry in Brazil. Products alternative to chemical acaricides have been investigated for tick control, and the use of plantderived products may be a promising approach. In the current study, we investigated the activity of the latex of Carica papaya L. fruit on...
Article
The aim of this study was to test nematicidal activity of the Euphorbia trigona latex on Meloidogyne incognita juveniles and to partially purify and characterize three proteases present in this latex. Three distinct proteases were partially purificated from E. trigona latex. They were named here trigonin 1, 2 and 3. Their molecular weights were est...
Chapter
Biofilm is an interesting niche for fungi and essential for growth, pathogenicity, and resistance that form high challenges both in current trends and in the future in the management of fungal diseases. Fungi cause many problems for different organisms in several fields such as in agriculture, livestock, and public health. Fungicides are widely uti...
Article
Full-text available
The present paper reviewed publications on the nematocidal activity of edible mushrooms (EM) and their potential use as sustainable tools for the control of parasitic nematodes affecting agriculture and livestock industry. Nematodes are organisms living in the soil and animals’ guts where they may live as parasites severely affecting economically i...
Article
The aim of this study was to evaluate the combined use of different chemical (albendazole, ivermectin, glycerine and Vaseline) and biological ( Monacrosporium thaumasium ) compounds in the control of Ancylostoma caninum. Infective larvae of A. caninum were obtained from coprocultures of positive faeces from naturally infected dogs. We used 1% iverm...
Article
This study evaluated the germination capacity of Pochonia chlamydosporia (VC4) fungus after its passage through the gastrointestinal tract of domestic chickens and its interaction with Ascaridia galli and Heterakis gallinarum eggs. Twenty-two domestic chickens were divided in two groups: control group (G1) received shredded corn substrate without V...
Article
The in vitro effect of silver nanoparticles of the Duddingtonia flagrans filtrate enriched with chitin was evaluated on infective larvae of cyathostomins (L3). After biosynthesis, an assay was carried out with two experimental groups in microtubes, for a period of 24 hours: G1 (AgNP's-D. flagrans (43.4 μg/mL) + 120 L3) and G2 (distilled water + 120...
Chapter
Nematophagous fungi are usually investigated and used in biocontrol of phytonematodes; recently, these fungi were found in association to the rhizosphere of crop plants colonizing their roots. Associated with plants, nematophagous fungi may also synthesize phytohormones or benefit the absorption of phosphorus by the plants. On the other hand, entom...
Article
Rhabditis spp., is a nematode known to cause otitis externa, an infection difficult to control, in cattle reared within tropical regions. The objective of this study was to assess the combined use of ivermectin 1%, dimethyl sulfoxide 1% and mineral oil 100% containing nematophagous fungi of both Duddingtonia flagrans (AC001) and Monacrosporium thau...
Article
The aim of the present work was to evaluate the nematicidal potential of Flammulina velutipes and its spent mushroom compost. Additionally, the nematicidal activity of enzymes and metabolites was analyzed. Isolated F. velutipes and its SMC had significant nematicidal effect on Panagrellus sp. larvae. The percentages of reduction in relation to the...
Article
This study investigate to evaluate the acute anti-inflammatory activity of Mangifera indica L. leaf extract and mangiferin in the liver of rats fed a cafeteria diet. This study was a randomized longitudinal experimental study. The animals were divided in three groups – Control: cafeteria diet (CD); Extract: CD + leaf extract (250 mg/kg); and Mangif...
Article
Lynxacarus radovskyi are mites commonly found within domestic feline hair stems. The infested animal presents an opaque fur with a “salt and pepper” aspect. The contamination may occur by direct contact with other infected animals or by fomites, and the main diagnostic tool is the direct examination of the fur. The Feline Eosinophilic Granuloma Com...
Article
Full-text available
Background: Helminth parasites cause morbidity and mortality in both humans and animals. Most anthelmintic drugs used in the treatment of parasitic nematode infections act on target proteins or regulate the electrical activity of neurons and muscles. In this way, it can lead to paralysis, starvation, immune attack, and expulsion of the worm. Howeve...
Article
The objective of this study was to evaluate, in vitro, the colonization and destruction of ants of the genus Camponotus sp. by the ovicidal fungus Pochonia chlamydosporia (VC4 isolate), in the southeast region of Brazil. The insects used in the experiment were worker ants of the genus Camponotus sp., collected periodically in the environment and im...
Article
Full-text available
The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysi...
Article
Full-text available
The objective of this study was to purify, characterize, and phylogenetically and structurally analyze the dextranase produced by the fungus Pochonia chlamydosporia. Dextranase produced by the fungus P. chlamydosporia was purified to homogeneity in two steps, with a yield of 152%, purification factor of 6.84 and specific activity of 358.63 U/mg. It...
Article
Full-text available
ABSTRACT The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice. The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composi...
Article
The objective of this work was to optimize the total cellulase activity of the crude extract cocktails from five white rot fungi produced by solid-state fermentation, by means of the central composite design. The white rot fungi Pleurotus ostreatus PLO 06, Pleurotus eryngii PLE 04, Trametes versicolor TRAM 01, Pycnosporus sanguineus PYC 02 and Phan...
Chapter
Neglected tropical diseases (NTDs) are a group of tropical infections especially endemic to low-income populations from developing regions of Africa, Asia, and the Americas. Together, this group of tropical infections is responsible for an estimated value ranging from 500,000 to 1 million deaths per year and is also responsible for a higher global...
Article
The production and consumption of mushrooms has increased, resulting in large amount of residue from cultivation, called spent mushroom compost (SMC). This study aimed the active enzymes recovery from SMC of Hypsizygus marmoreus, Flammulina velutipes, Pleurotus eryngii, Lentinula edodes, Pleurotus ostreatus and Pleurotus sp. Results indicated that...
Article
Pleurotus eryngii is one of the most cultivated and consumed mushroom species in North Africa, Europe and Asia. Fungi from Pleurotus genus have demonstrated nematophagous activities, however most of the literature reports are focused on Pleurotus ostreatus. The aim of this work was to evaluate P. eryngii action on Panagrellus sp. and to evaluate th...
Article
The present work aimed to characterize the proteases present in Euphorbia milii latex and to evaluate the nematicidal potential of these enzymes. Results suggest that proteases present in E. milii latex concentrate samples are milin and miliin. Difference (p < 0.01) between larvae number in the different groups was found, with 65.59% and 96.46% Pan...
Article
Nematophagous fungi, such as Monacrosporium thaumasium (NF34), have a high potential to produce enzymatic pool containing protease with nematicidal activity. Thus, the aim of this study was to optimize the conditions that affect this pool production by the fungus M. thaumasium on solid medium and its nematicidal effect on Meloidogyne javanica eggs....
Article
Full-text available
The present study aimed to evaluate the action of Paecilomyces marquandii proteases on Ancylostoma spp L3. White halos in the zymogram confirmed the proteolytic action. Difference (p <0.01) between the number of L3 in the differents groups was found, with 41.4% of reduction of Ancylostoma spp. L3 before 24 hours.
Article
Full-text available
Platynosomum fastosum is a parasite that affects cats, and among these, domestic cats, especially those originating from areas with tropical and subtropical climates. It is the most common liver parasite of domestic cats and is usually located in the bile ducts and gall bladder, but may also be found in the small intestine and pancreas. Infection o...
Article
Full-text available
The biocontrol is proven effective in reducing in vitro and in situ free-living stages of major gastrointestinal helminths, allowing progress in reducing losses by parasitism, maximizing production, and productivity. This study aimed at evaluating the predatory activity of fungal isolates of Duddingtonia flagrans and Clonostachys rosea species and...
Article
Full-text available
The objective of this study was to assess antagonism of nematophagous fungi and species producers metabolites and their effectiveness on Haemonchus contortus infective larvae (L 3 ). Assay A assesses the synergistic, additive, or antagonistic effect on the production of spores of fungal isolates of the species Duddingtonia flagrans , Clonostachys r...
Article
Full-text available
Among the parasites of domestic and wild swine, Ascaris suum stands out; a nematode that can lead to growth retardation and reduction in weight gain due to its action, especially in young animals. The objective of this study was to test the ovicidal action of proteases from Pochonia chlamydosporia (VC4) on A. suum eggs in an assay with Petri dishes...
Article
Full-text available
Background: Gastrointestinal parasitoses have high rates of morbidity and mortality. Each year about 3.5 billion people are affected by these diseases and 65,000 of them die, mostly in developing countries due to lack of basic sanitation, malnutrition, and poor access to medication. Thus, they constitute an important public health problem due to ca...
Article
Full-text available
The objective of this study was to evaluate the infectivity of Toxocara canis eggs after interacting with isolated nematophagous fungi of the species Duddingtonia flagrans (AC001) and Pochonia chlamydosporia (VC4), and test the predatory activity of the isolated AC001 on T. canis second stage larvae after 7 days of interaction. In assay A, 5000 emb...
Article
Full-text available
Nematophagous fungi produce chitinases that may be important in the process of infection of eggs and larvae of nematodes. This study aimed to produce, purify, characterise and test the nematicidal action of extracellular chitinases produced by Monacrosporium thaumasium on Panagrellus redivivus. Mycelia from M. thaumasium were used to inoculate a so...
Article
Full-text available
Several chitinases with nematicidal action of these fungi have been identified in recent years, however, little is known about which nutritional factors from the culture medium are significant for its mass production. The aim of this study was to perform the statistical screening of the components of the culture medium for chitinase production by n...
Chapter
Productivity within sheep breeding is highly affected by great parasite volumes, especially during lactation and gestation processes. This paper is focused on the evaluation of all main hematological, biochemical, and coproparazitologic changes, besides larval designation found within pastures of Dorper breed sheeps and females through peripartum a...
Article
Full-text available
The predatory nematophagous fungus Arthrobotrys sinensis (SF53) produces three proteases with nematicidal activity when grown on solid media culture. However, the proteolytic profile produced by this fungus, when grown in liquid culture medium remains unknown. Thus, the objective of this work was to evaluate the production of proteases from nematop...
Article
The present work used Plackett–Burman experimental design design to assess the influence of enzymes of nematophagous fungi versus Strongyloides westeri and trichostrongylides larvae and Platynosomum fastosum eggs. The variables studied in the Plackett–Burman design were the proteases and chitinases of AC001 or VC4 as destructive agents of S. wester...
Article
Full-text available
The ovicidal action of the nematophagous fungus Pochonia chlamydosporia (VC4) was evaluated on Dioctophyma renale eggs under laboratory conditions (Assay A). Next, the enzymatic action of proteases and chitinases produced by P. chlamydosporia (VC4) was evaluated on D. renale eggs, under laboratory conditions (Assay B). At the end of the experiment,...
Article
Full-text available
Biological control of gastrointestinal nematodiasis in ruminants is an alternative to reduce the number of infective larvae. The fungal isolates predatory activity preservation is a basic requirement for the success of this control type. The aim of this work is to evaluate the predatory capacity of the fungus Arthrobotrys robusta (isolate I-31), pr...
Article
Full-text available
The sugarcane industry has huge potential for biorefinery concept application, given its development in recent years. In this context, cane sugar straw has become an attractive raw material for biofuel production. This study aims to investigate the chemical composition of cane sugar straw from different regions of Brazil, and to optimize a hydrothe...
Article
Full-text available
The sugarcane industry has huge potential for biorefinery concept application, given its development in recent years. In this context, cane sugar straw has become an attractive raw material for biofuel production. This study aims to investigate the chemical composition of cane sugar straw from different regions of Brazil, and to optimize a hydrothe...
Article
Full-text available
The influence of casein and pH on the activity of the nematophagous fungus Duddingtonia flagrans (AC001) on trichostrongylide larvae was evaluated. A ‘positive influence’ was observed contributing to the reduction of 63% in the average number of recovered L3 in the media supplemented with casein and pH 7.0.