Filipa Esteves

• Postdoc Researcher at Algarve Biomedical Center Research Institute

Norovirus (NoV) and rotavirus group A (RVA) are major agents of acute gastroenteritis worldwide. This study aimed to investigate their epidemiological profile in Portuguese elderly living in long‐term care facilities and to assess the host genetic factors mediating infection susceptibility. From November 2013 to June 2015, 636 faecal specimens from...

The unprecedented rise in life expectancy observed in the last decades is leading to a global increase in the ageing population, and age-associated diseases became an increasing societal, economic, and medical burden. This has boosted major efforts in the scientific and medical research communities to develop and improve therapies to delay ageing a...

Cardiovascular diseases (CVDs) are pointed out by the World Health Organization (WHO) as the leading cause of death, contributing to a significant and growing global health and economic burden. Despite advancements in clinical approaches, there is a critical need for innovative cardiovascular treatments to improve patient outcomes. Therapies based...

As life expectancy continues to increase worldwide, age-related dysfunction will largely impact our societies in the future. Aging is well established to promote the deterioration of cognitive function and is the primary risk factor for the development of prevalent neurological disorders. Even in the absence of dementia, age-related cognitive decli...

The World Health Organization predicts that by 2050, 2.1 billion people worldwide will be over 60 years old, a drastic increase from only 1 billion in 2019. Considering these numbers, strategies to ensure an extended “healthspan” or healthy longevity are urgently needed. The present study approaches the promotion of healthspan from an epigenetic pe...

Glioblastoma (GBM) is a relatively rare but highly aggressive form of brain cancer characterized by rapid growth, invasiveness, and resistance to standard therapies. Despite significant progress in understanding its molecular and cellular mechanisms, GBM remains one of the most challenging cancers to treat due to its high heterogeneity and complex...

Aging is characterized by a progressive decline in physiological function and underlies several disabilities, including the increased sensitivity of cells and tissues to undergo pathological oxidative stress. In recent years, efforts have been made to better understand the relationship between age and oxidative stress and further develop therapeuti...

Human ageing is a complex, multifactorial process characterised by physiological damage, increased risk of age-related diseases and inevitable functional deterioration. As the population of the world grows older, placing significant strain on social and healthcare resources, there is a growing need to identify reliable and easy-to-employ markers of...

PIK3CA mutations are the most common in breast cancer, particularly in the estrogen receptor-positive cohort, but the benefit of PI3K inhibitors has had limited success compared with approaches targeting other less common mutations. We found a frequent allelic expression imbalance between the missense mutant and wild-type PIK3CA alleles in breast t...

Introduction: Translation of genome-wide association study (GWAS) findings into preventive approaches is challenged by the identification of the causal risk variants and the understanding of the biological mechanisms by which they act. We present using allelic expression (AE) ratios to perform quantitative case-control analysis as a novel approach...

A bstract Translation of GWAS findings into preventive approaches is challenged by identifying the causal risk variants and understanding their biological mechanisms. We present a novel approach using AE ratios to perform quantitative case-control analysis to identify risk associations, causal regulatory variants, and target genes. Using the breast...

PIK3CA mutations are the most common in breast cancer, particularly in the estrogen receptor positive cohort, but the benefit of PI3K inhibitors has had limited success compared with approaches targeting other less common mutations. We found allelic imbalances in the expression of PIK3CA in normal breast tissue and mapped a germline candidate regul...

1st Oral Communication Award

Genome-wide association studies (GWAS) identified a large number of risk loci to be associated with common complex diseases. The difficulty remains however in pinpointing the causative genetic variants within those risk loci, and in uncovering the underlying molecular mechanisms. Increasing evidence supports a cis-regulatory role for most of the ri...

21th Reunion of the Portuguese Society of Human Genetics (SPGH) - Best Oral Communication in Basic Research

Fundação AstraZeneca Innovate Competition - Translational Research Project Innovate Oral Award

Genetic variability of 19 isolates of Grapevine leafroll-associated virus 2 (GLRaV-2) from Portuguese grapevine cultivars was characterized by sequencing the entire capsid protein (CP) gene of the virus. Global phylogenetic analysis of the CP gene, which included nucleotide sequences obtained in this study and complete homologous sequences from Gen...

Grapevine leafroll disease (GLD) is the most important disease of grapevines, occurring in every grape-growing country. GLRaV-3 is the type member and the most widespread of the Ampeloviruses implicated in GLD. In turn, infectious degeneration, another most relevant and widespread grapevine virus diseases, is induced by distorting and chromogenic s...

Grapevine virus A (GVA) is one of the most widespread viruses in vineyards worldwide, known for more than 30 years. GVA is implicated in the rugose wood complex, with several natural insect vectors. Routine detection is done by DAS-ELISA with commercial antibodies. Due to the high genetic heterogeneity of GVA, reliable molecular detection and typif...

To date eight Grapevine leafroll-associated viruses (GLRaV-1,-2,-3,-4,-5,-6,-7,-9) have been implicated in grapevine leafroll disease (GLRD). Although GLRaV-2 is known to occur in Europe not much information is available on the incidence and variability of the virus in field situations, in contrast to other world regions from where robust evidence...

Grapevine leafroll disease (GLRD) is one of the most important virus diseases of grapevines worldwide, causing major economical impact. The disease has a complex aetiology and currently eleven phloem-limited viruses, termed in general Grapevine leafroll-associated virus (GLRaVs), have been identified. Two of the GLRaVs, GLRaV- 1 and GLRaV-3, ar...

view at http://web.pppmb.cals.cornell.edu/fuchs/icvg/

view at http://web.pppmb.cals.cornell.edu/fuchs/icvg/

Grapevine leafroll-associated virus 1 (GLRaV-1) is implicated in the grapevine leafroll disease (LRD), a syndrome of complex aetiology and great impact in grapevine yield 4. All serologically diverse viruses implicated in LRD have been assigned to the family Closteroviridae, the only virus family that encodes a 70-kDa heat-shock protein homolog (...

A comparison of 15 field isolates of grapevine leafroll-associated virus 5 (GLRaV-5) was conducted, based on the analysis of nucleotide sequences of two viral ORFs: the coat protein (CP) and the heat shock protein 90 homolog (HSP90h). After amplification and cloning, the population of viral sequences was analyzed for each isolate, revealing the wit...

Old grapevine varieties vineyards give us a pre-sanitary selection insight view over the situation of viruses’ infections in grapevine and are an enormous asset to study grapevine viruses variability with the added inputs of been also monitored for other viticulture characteristics and all plants been in equal climatic conditions. A Portuguese coll...

Nucleotide sequences, homologous to a partial fragment of the putative capsid protein gene or capsid protein duplicate of Grapevine leafroll-associated virus 8 (GLRaV-8), were obtained by RT-PCR of RNA independently extracted from distinct grapevine varieties. Alignment of the partial deduced amino acid sequences with the only sequence available at...

For three genomic regions of the GLRaV-3, respectively HSP70h (heat-shock protein 70), HSP90 (heat-shock protein 90) and CP (capsid protein), primers were designed and a collection of variants obtained through amplification, cloning and sequencing. The genetic variability of each region was analyzed, compared and discussed in terms of phylogenetic...

Forty eight new sequences of the GLRaV-1 capsid protein (CP) gene were obtained from seven Portuguese grapevine varieties and a total of eleven plants. Three of the varieties had been found negative for GLRAV-1 by DAS-ELISA testing, in consecutive years, with different commercially available antisera. Alignement of the deduced amino acids sequences...

Eight capsid protein (CP) gene complete sequences and twenty four HSP70h gene partial sequences of Grapevine leafroll-associated virus 2 were obtained from a Portuguese vineyard. In silico analysis of those and of all the corresponding nucleotide sequences available at GenBank revealed the existence of at least six variant groups, concurrent for th...