About
194
Publications
25,665
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
2,702
Citations
Citations since 2017
Introduction
Skills and Expertise
Publications
Publications (194)
The aim of this multicentre project (seven hospitals across the Spanish National Health Service) was to study the phenotypic and genotypic susceptibility of C. trachomatis to the main antimicrobials used (macrolides, doxycycline, and quinolones) in isolates from patients with clinical treatment failure in whom reinfection had been ruled out. During...
Mutational analysis of SARS-CoV-2 can quantify their relative importance over time, enable the dominant mutations to be identified and facilitate near real-time detection, comparison, and tracking of evolving variants. Collected samples in Asturias an autonomous community of Spain with a large aged population, and high levels of migration and touri...
Microbiological diagnosis by using commercial multiplex quantitative PCR systems provides great advantages over the conventional culture. In this work, the Biofire FilmArray Pneumonia Panel Plus (FAPP+) was used to test 144 low respiratory tract samples from 105 COVID-19 patients admitted to an Intensive Care Unit (ICU), detecting 78 pathogens in 5...
Silver nanoparticles (AgNPs) play an important role in the medical field due to their potent antimicrobial activity. This, together with the constant emergence of resistance to antimicrobial drugs, means AgNPs are often investigated as an alternative to solve this problem. In this article, we analyzed the antifungal and antiamoebic effects of a rec...
The most common causal agents of fungal keratitis are yeasts of the Candida genus. Adhesion constitutes the first stage of pathogenesis. Previous studies have shown that glycosaminoglycans from the corneal cell surface play an essential role in bacterial keratitis, although little is known about their role in fungal infections. The objective of thi...
Background
Cell surface glycosaminoglycans (GAGs) participate in many physiological and pathological processes, including infections and inflammatory response. Acne is a common chronic inflammatory skin disorder that affects the pilosebaceous unit and has a multifactorial etiology, including bacterial colonization of the hair follicle. This study a...
One of the most important books written, and most cited, is Susan Sontags book: Illness as Metaphors. AIDs and its Metaphors. Disease, and infections in particular, have been present for centuries in works of world literature. Infections appear in these works generally in the context of the time they were written, sometimes anecdotally and sometime...
One of the most important books written, and most cited, is Susan Sontags book: Illness as Metaphors. AIDs and its Metaphors. Disease, and infections in particular, have been present for centuries in works of world literature. Infections appear in these works generally in the context of the time they were written, sometimes anecdotally and sometime...
One of the most important books written, and most cited, is Susan Sontags book: Illness as Metaphors. AIDs and its Metaphors. Disease, and infections in particular, have been present for centuries in works of world literature. Infections appear in these works generally in the context of the time they were written, sometimes anecdotally and sometime...
Given the high prevalence of imported diseases in immigrant populations, it has postulated the need to establish screening programs that allow their early diagnosis and treatment. We present a mathematical model based on machine learning methodologies to contribute to the design of screening programs in this population. We conducted a retrospective...
Background:
Scarce information is available on the resources to deal with the Sexually Transmitted Infections (STIs), both in the clinic and in the laboratory. The objective is to describe and know the reality of the clinics and laboratories that treat these infections in Spain.
Methods:
Cross-sectional observational study with data collection t...
Uno de los más importantes libros escrito, y más citado, es el libro de Susan Sontag: «La enfermedad y sus metáforas. El sida y sus metáforas». La enfermedad, y las infecciones en particular, han estado presentes por siglos en las obras de la literatura mundial. Las infecciones aparecen en estas obras en el contexto generalmente del tiempo en que f...
Uno de los más importantes libros escrito, y más citado, es el libro de Susan Sontag: «La enfermedad y sus metáforas. El sida y sus metáforas». La enfermedad, y las infecciones en particular, han estado presentes por siglos en las obras de la literatura mundial. Las infecciones aparecen en estas obras en el contexto generalmente del tiempo en que f...
Objective To assess the loop-mediated isothermal amplification (LAMP) to detect cell-free DNA from Streptococcus pneumoniae in urine samples from children with pneumococcal pneumonia.
Methods LAMP reactions using four primers (backward inner primer, forward inner primer, B3, and F3) targeting conserved regions of the S. pneumoniae ply gene and DNA...
Background
Intensive care unit workers are at high risk of acquiring COVID-19 infection, especially when performing invasive techniques and certain procedures that generate aerosols (< 5 μm). Therefore, one of the objectives of the health systems should implement safety practices to minimize the risk of contagion among these health professionals. M...
Molecular epidemiology of circulating clinical isolates is crucial to improve prevention strategies. The Spanish Working Group on multidrug resistant tuberculosis (MDR-TB) is a network that monitors the MDR-TB isolates in Spain since 1998. The aim of this study was to present the study of the MDR-TB and extensively drug-resistant tuberculosis (XDR-...
Background Intensive care unit workers are at high risk of acquiring COVID-19 infection, specially when performing invasive techniques and certain procedures that generate aerosols (<5 µm). Therefore, one of the objectives of the health systems should be to implement safety practices to minimize the risk of contagion among these health professional...
Background: Intensive care unit workers are at high risk of acquiring COVID-19 infection, especially when performing invasive techniques and certain procedures that generate aerosols (<5 µm). Therefore, one of the objectives of the health systems should implement safety practices to minimize the risk of contagion among these health professionals. M...
Resumen
Introducción
Hay escasa información sobre los recursos disponibles tanto en las consultas como en el laboratorio para hacer frente a las infecciones de transmisión sexual (ITS). El objetivo es describir y conocer la realidad de las consultas y laboratorios que atienden las ITS en España.
Métodos
Estudio observacional transversal con obten...
This review focuses on the fictional literature in which the Spanish flu is represented either as an anecdotal or as a historical aspect and the effect on the author or fictional character. We examine this sociocultural period in the press and mainly in Anglo-Saxon literary works and from other countries, including Spanish and Latin American litera...
Background:
The emergence and expansion of antibiotic resistance makes it necessary to have alternative anti-infective agents, among which silver nanoparticles (AgNPs) display especially interesting properties. AgNPs carry out their antibacterial action through various molecular mechanisms, and the magnitude of the observed effect is dependent on...
Preservation of strains is important to further studies and characterization; we have shown a technique of long-term preservation of Neisseria spp. and Haemophilus spp. by freezing cotton tip swabs impregnated with fastidious organisms at −80 °C without the addition of cryoprotectants.
Background: The emergence and expansion of antibiotic resistance makes it necessary to have alternative anti-infective agents, among which silver nanoparticles (AgNPs) display especially interesting properties. AgNPs carry out their antibacterial action through various molecular mechanisms, and the magnitude of the observed effect is dependent on m...
We describe the infections that appeared in the life and work of John Donne (1572-1631), the English metaphysical poet, mainly the exanthematic typhus that suffered and gave arise to his work Devotions upon emergent occasions, and several steps in my sickness. We discuss the vector of transmission of this disease, in comparison of other infections...
Nosocomial Central Nervous System (CNS) infections are often serious complications of neurosurgical procedures. Their diagnosis is complex and frequently based on microbiological culture. The aim of this work was to evaluate the effectiveness of the FilmArray® Blood Culture Identification (BCID) panel, a multiplex PCR designed to identify the most...
Resumen
La sífilis es una infección de transmisión sexual causada por Treponema pallidum subsp. pallidum, cuya incidencia está aumentando en España y en el resto del mundo. El diagnóstico se basa fundamentalmente en la serología, puesto que el diagnóstico directo mediante microscopía de campo oscuro presenta dificultades que limitan su generalizaci...
Background The emergence and expansion of antibiotic resistance makes it necessary to have alternative anti-infective agents, among which silver nanoparticles (AgNPs) display especially interesting properties. AgNPs carry out their antibacterial action through various molecular mechanisms, and the magnitude of the observed effect is dependent on mu...
Background: The emergence and expansion of antibiotic resistance makes it necessary to have alternative anti-infective agents, among which silver nanoparticles (AgNPs) display especially interesting properties. AgNPs carry out their antibacterial action through various molecular mechanisms, and the magnitude of the observed effect is dependent on m...
Background: The emergence and expansion of antibiotic resistance makes it necessary to have alternative anti-infective agents, among which silver nanoparticles (AgNPs) display especially interesting properties. AgNPs carry out their antibacterial action through various molecular mechanisms, and the magnitude of the observed effect is dependent on m...
Background: The emergence and expansion of antibiotic resistance makes it necessary to have alternative anti-infective agents, among which silver nanoparticles (AgNPs) display especially interesting properties. AgNPs carry out their antibacterial action through various molecular mechanisms, and the magnitude of the observed effect is dependent on m...
Streptococcus pneumoniae shows more than 90 capsular serotypes that can be distinguished by their reactivity against antisera. The main objective of this work was the development of a molecular method for serotyping without the use of antisera. A computer program containing an algorithm was used to search in a database for potentially useful enzyme...
Objectives:
The aim of this cross-sectional study was to describe the results of a systematic serological screening programme for strongyloidiasis.
Methods:
A prospective serological screening programme for strongyloidiasis was performed between 2009 and 2014 for all immigrant patients attending the Tropical Medicine Unit. Three formalin-ether c...
The latest advances in the vaginal microbiome and molecular diagnosis of bacterial vaginosis have allowed for a better knowledge of this entity, characterising aspects of its pathogenesis and the establishment of the vaginal biolayer, the models and new theories of its aetiology, how it is transmitted, with it being considered nowadays as a probabl...
Objective:
To evaluate the clinical impact of Meningitis/Encephalitis FilmArray® panel for the diagnosis of cerebral nervous system infection and to compare the results (including time for diagnosis) with those obtained by conventional microbiological techniques.
Methods:
A prospective observational study in an Intensive Care Unit of adults from...
Introduction
The characteristics of D. fragilis infection are described, with special focus on the clinical and epidemiological aspects.
Materials and methods
A retrospective and descriptive study was performed, including all the patients with Dientamoeba fragilis infection who attended a specialized unit between January 2012 and December 2017. PC...
Western blot analysis is widely used for detecting protein expression, analysis of protein–protein interactions, and searching for new biomarkers. Also, it is a diagnostic tool used for detection of human diseases and microorganism infections. Some Streptococcus pneumoniae proteins are important virulence factors and a few of them are diagnostic ma...
The latest advances in the vaginal microbiome and molecular diagnosis of bacterial vaginosis have allowed for a better knowledge of this entity, characterising aspects of its pathogenesis and the establishment of the vaginal biolayer, the models and new theories of its aetiology, how it is transmitted, with it being considered nowadays as a probabl...
Background:
Glycosaminoglycans (GAGs) are essential in many infections, including recurrent bacterial respiratory infections, the main cause of mortality in cystic fibrosis (CF) patients.
Methods:
Using a cellular model of healthy and CF lung epithelium, a comparative transcriptomic study of GAG encoding genes was performed using qRT-PCR, and th...
Introduction:
The characteristics of D. fragilis infection are described, with special focus on the clinical and epidemiological aspects.
Materials and methods:
A retrospective and descriptive study was performed, including all the patients with Dientamoeba fragilis infection who attended a specialized unit between January 2012 and December 2017...
Introduction
The results of a study on the household contacts of patients with D. fragilis infection are presented.
Methods
A prospective, descriptive study was carried out on all Dientamoeba fragilis-infected patients treated at the Tropical Medicine Unit of Hospital Universitario Central de Asturias between 2012- 2017 and their household contact...
El balance de nutrientes de las actividades agropecuarias en la provincia de Buenos Aires, Argentina: una herramienta para un manejo sustentable de los agroecosistemas a escala regional The nutrient balance of agricultural activities in the province of Buenos Aires,
El balance de nutrientes de las actividades agropecuarias en la provincia de Buenos Aires, Argentina: una herramienta para un manejo sustentable de los agroecosistemas a escala regional The nutrient balance of agricultural activities in the province of Buenos Aires,
El balance de nutrientes de las actividades agropecuarias en la provincia de Buenos Aires, Argentina: una herramienta para un manejo sustentable de los agroecosistemas a escala regional The nutrient balance of agricultural activities in the province of Buenos Aires,
RESUMEN
En el periodo de 1915 a 1924 se describieron brotes de carbunco por Bacillus anthracis debido a la contaminación de las brochas de afeitar que llegaban a Europa y Estados Unidos desde zonas como Japón, China o Rusia. Las brochas se hacían con pelo de tejón y después para abaratarlas con pelo de caballo y de otros animales. La I Guerra Mundi...
Blood stream infections are serious conditions associated with high morbi-mortality. In this study, the new Sepsis Flow Chip (SFC) assay for identification of Gram-negative ) bacteria and their antimicrobial resistance genes was evaluated in positive blood cultures (BCs). SFC is a microarray with a broad panel comprising the most frequent causative...
Background
Keratoconus has classically been defined as a noninflammatory disorder, although recent studies show elevated levels of inflammatory markers suggesting that keratoconus could be, at least in part, an inflammatory condition. Heparanase upregulation has been described in multiple inflammatory disorders. In this article, we study the differ...
Introduction:
The results of a study on the household contacts of patients with D. fragilis infection are presented.
Methods:
A prospective, descriptive study was carried out on all Dientamoeba fragilis-infected patients treated at the Tropical Medicine Unit of Hospital Universitario Central de Asturias between 2012- 2017 and their household con...
Objective:
Infectious complications related to external ventricular shunt (ICREVS) are a main problem in neurocritical intensive care units (ICU). The aim of the review is to assess the incidence of ICREVS and to analyse factors involved.
Methods:
Retrospective analysis, adult polyvalent ICU in a third level reference hospital. Patients carrying...
Sexually transmitted infections (STIs) are responsible for an enormous burden of morbidity and mortality. Worldwide, millions of cases of STIs, such as syphilis, chlamydia, or gonorrhoea occur every year, and there is now an increase in antimicrobial resistance in pathogens, such as gonococcus. Delay in diagnosis is one of the factors that justifie...
Background
Lower respiratory infections are among the top ten causes of death worldwide. Since pathogen to cell adhesion is a crucial step in the infection progress, blocking the interaction between eukaryotic receptors and bacterial ligands may enable the pathogenesis process to be stopped. Cell surface glycosaminoglycans (GAGs) are known to be me...
Objective:
Multidrug resistant (MDR) microorganisms represent a threat for patients admitted in Intensive Care Units (ICUs). The objective of the present study is to analyse the results of epidemiological surveillance cultures for these microorganisms in one of these units.
Methods:
General ICU. Retrospective analysis, descriptive statistics. An...
Carbapenemase-producing Enterobacteriaceae is increasing worldwide. Rectal screening for these bacteria can inform the management of infected and colonized patients, especially those admitted to intensive care units (ICUs). A laboratory developed, qualitative duplex real-time polymerase chain reaction assay for rapid detection of OXA-48-like and VI...
Purpose:
Keratoconus is a heterogeneous disease associated with a range of pathologies, including disorders that involve proteoglycans (PGs). Some PG alterations, mainly in keratan sulfate (KS), occur in keratoconus. In this article, we studied the differential expression of the genes encoding PGs in cells isolated from keratoconus patients and he...
Marine Actinobacteria are emerging as an unexplored source for natural product discovery. Eighty-seven deep-sea coral reef invertebrates were collected during an oceanographic expedition at the submarine Avilés Canyon (Asturias, Spain) in a range of 1500 to 4700 m depth. From these, 18 cultivable bioactive Actinobacteria were isolated, mainly from...
Sexually transmitted infections (STIs) are responsible for an enormous burden of morbidity and mortality. Worldwide, millions of cases of STIs, such as syphilis, chlamydia, or gonorrhoea occur every year, and there is now an increase in antimicrobial resistance in pathogens, such as gonococcus. Delay in diagnosis is one of the factors that justifie...
The epithelium of the cornea is continuously exposed to pathogens, and adhesion to epithelial cells is regarded as an essential first step in bacterial pathogenesis. In this article, the involvement of glycosaminoglycans in the adhesion of various pathogenic bacteria to corneal epithelial cells is analyzed. All microorganisms use glycosaminoglycans...
All parts of our body having communication with the external environment such as the skin, vagina, the respiratory tract or the gastrointestinal tract are colonized by a specific microbial community. The colon is by far the most densely populated organ in the human body. The pool of microbes inhabiting our body is known as “microbiota” and their co...
Clostridium difficile is recognised worldwide as the main cause of infectious bacterial antibiotic-associated diarrhoea in hospitals and other healthcare settings. The aim of this study was to first survey C. difficile prevalence during the summer of 2014 at the Central University Hospital of Asturias (Spain). By typing the isolates obtained, it wa...
A new protocol for Enterobacteriaceae identification and detection of carbapenemase-producing isolates from blood cultures by combining MALDI-TOF MS and the Carba NP test has been evaluated. Bacterial identification was correct in 129 of the 130 isolates tested while the Carba NP detected 28 out of the 29 carbapenemase producers.
Objectives: Dientamoeba fragilis is a pathogenic protozoan of the human gastrointestinal tract with a worldwide distribution, which has emerged as an important and misdiagnosed cause of chronic gastrointestinal illnesses such as diarrhea and ‘irritable-bowel-like’ gastrointestinal disease. Very little research has been conducted on the use of suita...
Resumen
Objetivos
Mejorar la seguridad del paciente es necesario en las unidades de cuidados paliativos donde no hay datos de incidentes y eventos adversos. Se analizaron los tipos de incidentes/eventos adversos, su frecuencia y gravedad en la Unidad de Cuidados Paliativos de un hospital de agudos geriátrico con el objetivo de introducir medidas q...
Infectious diseases remain an important global health problem. The interaction of a wide range of pathogen bacteria with host cells from many different tissues is frequently mediated by proteoglycans. These compounds are ubiquitous complex molecules which are not only involved in adherence and colonization, but can also participate in other steps o...
The OXA-48 K-Se T, a new immunochromatographic assay for rapid detection of OXA-48-producing Enterobacteriaceae, has been evaluated in a Spanish Hospital during a three-month period. A collection of 100 Enterobacteriaceae including 79 isolates producing OXA-48 has been tested. 100% sensitivity and specificity were obtained.
This chapter reviews the epidemiology, pathogenesis, clinical features, diagnosis, treatment, and prevention of Chlamydia trachomatis infection. The Chlamydiae share a unique biphasic developmental cycle. The elemental body (EB) is the infectious form, infects eukaryotic host cells, and can survive for only a limited period of time outside the host...
Objectives
To assess the frequency, severity, and preventability of adverse events (AEs) detected using the Global Trigger Tool (GTT) in an acute geriatric hospital.DesignA 6-year retrospective study.SettingAn urban Spanish acute geriatric teaching hospital of 200 beds.ParticipantsTen randomly selected clinical records were chosen every fortnight f...
C. trachomatis infection is the leading cause of STIs worldwide. In a high percentage of people, most of the infections run asymptomatic course. Due to this asymptomatic state, those subjects will probably not be treated, may serve as an important source of transmission and are at risk for late complications. In this chapter we determine the preval...
There are no accurate data regarding the real prevalence of Chlamydia trachomatis infection in Spain. Our aim was to determine the prevalence of C. trachomatis infections and the risk factors for acquiring them among 1,048 young (15--24 years old) inhabitants of Laviana.
The study was completed in the period between 1st November 2010 and 31st Decem...
Objectives:
Whereas several studies about patient falls have provided data for long-term healthcare institutions, less information is available for acute care centres. The objective was to analyze the characteristics of the patient falls and associated factors, and the effectiveness of the lower beds' height to reduce the frequency and the harms o...
We review here different state-of-the-art molecular methods currently used in the diagnosis of sexually transmitted infections.
Background:
Information is scarce on the impact of the clinical electronic record on the frequency and severity of medication errors in acute geriatric patients.
Material and methods:
An analytical and descriptive pre-post study was conducted on the implementation of computerized provider order entry systems (CPOE), over a 6 year period. A volun...