Fernando Pineda

• PhD PharmD
• Head of Department at Inmunotek SL

Background: In Portugal, dust mite (DM) allergens, namely those of Dermatophagoides pteronyssinus (Dp), are the most prevalent ones. Aim: to analyse component-resolved diagnosis (CRD) to Dp (rDer p 1, rDer p 2, rDer p 10 and rDer p 23) in patients with respiratory allergy to DM, and possible relationship with clinical severity and geographical area...

La alergia a la lapa produce síntomas mayoritariamente graves en forma de anafilaxia también en niños. • La alergia a la lapa se puede considerar una causa de anafilaxia por alergia alimentaria en la edad infantil en nuestra área, que al igual que otras clases de mariscos y los frutos secos, suele persistir durante toda la vida.

This study examines how climate change is reshaping airborne allergens, driving increases in allergic diseases. By comparing allergen sensitivities in Tenerife, Spain, and Lima, Peru—regions with similar climates but different socio-economic conditions—it finds Spanish participants, especially those with asthma, are more broadly sensitized to aller...

Seafood is a vital source of nutrients, and its consumption has grown globally. Shellfish is a major cause of food allergy and anaphylaxis worldwide, affecting an estimated 0.5-2.5% of the general population. Most basic and clinical research has traditionally focused on crustaceans, leaving the characteristics of mollusk allergy poorly understood....

Food allergies have increased significantly in recent decades, with shellfish being a leading cause of food allergy and anaphylaxis worldwide, affecting both children and adults. The prevalence of shellfish allergies is estimated to be approximately 0.5-2.5% of the general population, varying significantly by geographical location, age, and consump...

In the recent years, several important advances have been made in the diagnosis of allergy using molecular techniques. The aetiological diagnosis of allergy using molecular components of allergens allows a more precise definition of the patient's IgE repertoire. Precision medicine is a structural model aimed at personalising healthcare and places t...

Allergen immunotherapy (AIT) with aeroallergens is the only disease-modifying treatment for patients with different allergic conditions. Despite the effectiveness of AIT having been proven in both randomized controlled trials and real-world studies, it remains underused in less than 10% of subjects with allergic rhinitis (AR) and/or asthma (A). We...

Allergen immunotherapy (AIT) with aeroallergens is the only disease modifying treatment for patients with different allergic conditions. Despite the effectiveness of AIT has been evidenced in both randomized controlled trials and real word studies, it remains underused in less than 10% of subjects with allergic rhinitis (AR) and/or asthma (A). We a...

Climate change and exposure to environmental pollutants play a key role in the onset and aggravation of allergic diseases. As different climate-dependent patterns of molecular immuno-globulin E (IgE) reactivity have been regionally described, we sought to investigate the evolving allergen exposome in distinctive allergic phenotypes and subtropical...

Fungal allergy is the third most frequent cause of respiratory pathologies and the most related to a poor prognosis of asthma. The genera Alternaria and Cladosporium are the most frequently associated with allergic respiratory diseases, with Alternaria being the one with the highest prevalence of sensitization. Alternaria alternata is an outdoor fu...

Climate change and exposure to environmental pollutants play a key role in the onset and aggravation of allergic diseases. As different climate-dependent patterns of molecular immunoglobulin E (IgE) reactivity have been regionally described, we sought to investigate the evolving allergen exposome in distinctive allergic phenotypes, and subtropical...

Background: The development of Precision Allergy Molecular Diagnosis (PAMD@) tools contributes to epidemiologic and diagnostic research concerning the determination of specific IgE (sIgE) against different allergenic sources in each geographical area. Excluding mites as the main local prevailing allergen, the objective of the present study was to d...

Introducción Nuestro objetivo es identificar el perfil molecular y la distribución de los alérgenos de los ácaros del polvo doméstico y de los ácaros del almacenamiento en una población seleccionada de niños con asma persistente de moderada a grave. Material y Métodos: Se seleccionaron 22 pacientes pediátricos no consecutivos sensibilizados a cual...

Background/objective: The objective of this study was to describe the molecular sensitization profile of mite allergy in an area with a high environmental exposure of house dust mites (HDM) and storage mites. Methods: Skin prick tests were performed with standardized extracts (DIATER, Madrid, Spain). A specific commercial molecular panel (MADx)...

Storage mites (SM) may induce allergic respiratory symptoms in sensitized individuals, in both rural and urban settings. The relationship among specific IgE reactions to determined groups of SM allergens in the coincident asthma pheno-endotypes has not yet been investigated. We aimed to study a Precision Allergy Molecular Diagnosis (PAMD@) model to...

Atopic dermatitis (AD) endotyping might be important for developing personalized diagnostic and therapeutic strategies to the different phenotypes. The current study investigated the IgE molecular profile to Dermatophagoides pteronyssinus (D. pteronyssinus) in a subset of patients afflicted with varying severity stages of atopic dermatitis in a subtr...

Atopic dermatitis (AD) is characterized by a disrupted skin barrier, and a polarized type 2 helper T-cell (Th2)-mediated immune responses to numerous environmental antigens. As the sensitization profile to both house dust mites (HDM) and storage mites (SM) may differ depending on the specific geographical locations and the underlying allergic disea...

Patrones moleculares de sensibilización a los ácaros de almacenamiento en el asma persistente en condiciones climáticas subtropicales. Objetivo / Introducción La relevancia de los ácaros de almacenamiento (AA) en la patogénesis de la alergia respiratoria sigue siendo incierta. Investigamos el perfil molecular serológico de una población asmática se...

Background: Mites are the most prevalent source of indoor allergens. The aim of the present study is based on a component resolved diagnosis (CRD) model approach to investigate the mite specific IgE sensitization profile to Dermatophagoides pteronyssinus and Blomia tropicalis and the performance outcomes of commercially available CRDin subjects wi...

Objective: Airway diseases, including allergic rhinitis, are prompted by specific IgE antibodies against airborne allergens and notably those derived from mites. The presented study focused on the specific IgE immediate response to Dermatophagoides pteronyssinus (D. pteronyssinus) mite major allergens and the corresponding pertinence of molecular...

Prevalencia y serodominancia del ácaro Dermatophagoides pteronyssinus en poblacion pediatrica con fenotipo de asma persistente moderado-grave Fernando Pineda, PhD1 ; Ruperto Gonzalez-Perez, MD PhD2; Miriam Castillo, PhD1; Paloma Poza-Guedes, MD, PhD2; Martina Aumayr3, PhD; Elena Mederos-Luis, MD2, Cristina Álava-Cruz2, MD, Peter Forstenlechner3, M...

Background: The association among the IgE responses to prevailing groups of house dust mite (HDM) allergens in the concurrent asthma phenotypes has not been determined. Objective: The aim of the present study lays on a component-resolved diagnosis (CRD) model to investigate the mite molecular signature in subjects with type-2 inflammation asthma...

Objective: To analyze component resolved diagnosis to Dermatophagoides pteronyssinus (Der p) in patients with respiratory allergy and its relationship with clinical severity in different geographical areas. Methods: 217 patients (mean age 25.85±12.7 years; 51.16% females) were included, selected from 13 centers in Portugal (5 from North, n=65)....

Background Occupational contact urticaria due to inhalation of legume pest allergens has rarely been described. Objective To determine the cause of an outbreak of airborne contact urticaria, asthma and anaphylaxis in farmers and agronomists studying the disinsectization of dried peas. Peas were treated with aluminum phosphide (EPA). Methods Extra...

Occupational asthma/rhinitis/urticaria due an until know unknown high-mollecular allergen source: pig semen

RATIONALE: Dog allergens are a common cause of allergic sensitization and triggering respiratory symptoms worldwide. The knowledge of the composition of the dog epithelia and the structure of the allergens that compose it is a prerequisite to carry out more accurate diagnosis and treatment. METHODS: A group of 30 patients with a history of modera...

RATIONALE: Sensitization profile to house dust mites (HDM) in respiratory allergy may differ depending on specific geographical areas. The role of mites is controversial concerning type 2 inflammation in asthma. The present study aims to characterize the immunological pattern in a selected asthma population focusing on their HDM sensitization. METH...

RATIONALE: The relevance of storage mites (SM) in the pathogenesis of respiratory allergy remains uncertain. We sought to investigate the serological molecular profile of a selected asthma population with a high environmental exposure focusing on SM sensitization. METHODS: We selected non-consecutive patients sensitized to SM (i.e. Blomia tropic...

Background: China and Spain are world leaders in the consumption of edible bulbs (garlic and onion), but there are few references to their capacity to cause allergic symptoms. The target was to study allergic sensitization and clinical symptoms associated with garlic and onion consumption in a large sample of allergic patients. Methods: An obser...

Background: Mastocytosis involves the abnormal proliferation of mast cells and clinical variability. In the case of anaphylaxis, the triggering antigen, often associated with Hymenoptera allergens, must be identified. The common fig (Ficus carica) requires the fig wasp (Blastophaga psenes) for pollination. Objective: We evaluated the ingestion o...

IntroductionA major clinical challenge in treating allergic contact dermatitis (ACD) is that the first line of treatment is based on the use of corticosteroids. In this study, we aimed to develop a formulation that is devoid of steroids. Methods We used mouse ears treated with dinitrofluorobenzene (DNFB) to induce ACD. The efficacy of the test form...

We report the case of a 36-year-old male pharmaceutical laboratory worker. On handling Spagulax® sachets whose content is a laxative called Plantago ovata, he immediately presented rhinoconjunctivitis. Methods. Specific allergy study included SDS-PAGE with Western Blot and specific nasal challenge to Plantago ovata extract. Results. Prick by prick...

Background: There are no studies on the cross-reactivity between Salsola kali and Salsola imbricata pollens. The main goal is to compare the degree of the cross-reactivity between Salsola kali and Salsola imbricata, and secondly to compare the various common allergen components between Salsola kali and Salsola imbricata. Methods: Serum from rhin...

Background: Adverse reactions to local anesthetics (LAs), especially esters, are not uncommon, but true allergy is rarely diagnosed. To our knowledge, currently there is no reliable method of determining IgE-mediated hypersensitivity to LAs and cocaine. Objective: To assess the clinical value of allergy tests (prick, IgE, challenges, and arrays)...

Background: Amoxicillin (AX) is the betalactam most often involved in IgE-mediated reactions and the diagnosis is mainly based on skin testing (ST) although without optimal sensitivity. We have produced a newly AX derivative, amoxicilloyl-poly-L-lysine (APL), and have analysed its IgE recognition by passive histamine release test (pHRT). Methods:...

Fungi are considered one of the most common allergens responsible for type I allergies. This allergic response can be triggered by inhalation of mold spores, skin contact, or ingestion of mushrooms. There are some case reports in the literature advocating an association between sensitization to mold spores and systemic reactions caused by ingestion...

Background: There has been an increase in the prevalence of hypersensitivity to Anisakis simplex. There are fish parasites other than Anisakis simplex whose allergenicity has not yet been studied. Objective: To assess IgE hypersensitivity caused by fish parasite allergens in patients with gastro-allergic symptoms after consumption of fish, shell...

Table of contents WORKSHOP 4: Challenging clinical scenarios (CS01–CS06) CS01 Bullous lesions in two children: solitary mastocytoma S. Tolga Yavuz, Ozan Koc, Ali Gungor, Faysal Gok CS02 Multi-System Allergy (MSA) of cystic fibrosis: our institutional experience Jessica Hawley, Christopher O’Brien, Matthew Thomas, Malcolm Brodlie, Louise Michaelis C...

Introdução O cultivo e consumo de citrinos encontra-se generalizado a nível mundial mas estão relatados poucos casos de alergia. Descrevemos o caso de um produtor de limões com alergia ao limão e à flor de limoeiro. O soro do doente reconheceu uma série de proteínas compreendidas entre os 25-70 kDa da casca do limão e das diferentes partes da flor....

Tras la ingesta de pan de multicereales, sensación de " pinchazos " faríngeos y posterior disnea. En la cena volvió a ingerirlo con aumento de la disnea y sensación de cierre faríngeo, vértigos y sibilancias. Acudió a Urgencias donde precisó tratamiento con adrenalina, actocortina y prednisona 30 mg. Se analizó el pan multicereal ingerido por el pa...

Introdução: a alergia alimentar múltipla constitui uma ameaça para o doente e um desafio diagnóstico para o médico. Quando confirmada permanecem dúvidas sobre o aconselhamento e terapêutica para além da evicção dos alimentos implicados. Métodos: revisão de um caso clínico de alergia alimentar a diferentes fontes alergénicas.

Purpose: Hypersensitivity to fungi is associated with rhinoconjunctivitis and asthma. For some fungi, such as Alternaria alternata (A. alternata), the symptoms of asthma are persistent, increasing disease severity and the risk of fatal outcomes. There are a large number of species of fungi but knowledge of them remains limited. This, together with...

Table of contents Poster walk 11: miscellaneous drug hypersensitivity 2 (P92–P94, P96–P101) P92 16 years of experience with proton pump inhibitors (PPIs) Javier Dionicio Elera, Cosmin Boteanu, Maria Aranzazu Jimenez Blanco, Rosario Gonzalez-Mendiola, Irene Carrasco García, Antonio Alvarez, Jose Julio Laguna Martinez P93 Allergy evaluation of quinol...

Backgorund: Food allergy is a rare disorder among breastfeeding babies. Objective: Our aim was to identify responsible allergens in human milk. Methods: We studied babies developing allergic symptoms at the time they were breastfeeding. Skin prick tests (SPT) were performed with breast milk and food allergens. Specific IgE was assessed and IgE...

Background: Heroin and its contaminants may be an important source of allergens in young people. We present a case of severe endophthalmitis in a patient that also suffered from anaphylactoid symptoms (hypotension, urticaria, glottic oedema) whenever he ingested lemon. Methods: Prick tests with a battery of 42 aeroallergens including fruits and...

Background: Immediate hypersensitivity reactions to clavulanic acid (CLV) seem to be on the increase. Diagnosis is mainly based on skin testing and the drug provocation test (DPT), procedures that are not risk free. The aim of this study was to evaluate whether the histamine release test (HRT) could help evaluate patients with selective hypersensi...

Background: Heroin and its contaminants may be an important source of allergens in young people. We present a case of severe endophthalmitis in a patient that also suffered fromanaphylactoid symptoms (hypotension, urticaria, glottic oedema) whenever he ingested lemon. Methods: Prick tests with a battery of 42 aeroallergens including fruits and citr...

Soy (soybean) (Glycine max) is an edible legume belonging to the Fabaceae family. The seed contains around 20% oil and 38 to 40% protein. Consumption of soy, widespread in Asia and the US, has increased in Europe during the past years particularly [1]. Soybean oil is used in cooking and food formulations. The presence of protein in soy oil depends...