About
15
Publications
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Introduction
I am a Biochemist with a Master's in Biochemistry and a PhD in Microbiology from the University of Chile. I studied how the lipid A hydroxylation catalyzed by LpxO enzyme contributes to the interaction of Salmonella Enteritidis with murine macrophages in vitro and its virulence in mice. Later, I worked to determine if T3SSs effectors GtgE and SopD2 of Salmonella Typhimurium play a role in its virulence in the model amoeba Dictyostelium discoideum as previously characterized for mammalian hosts.
Current institution
Education
March 2018 - August 2024
Universidad de chile
Field of study
- Host-pathogen interaction of Salmonella with environmental amoebae
April 2015 - August 2018
Universidad de Chile
Field of study
- Impact of lipid A remodelling on Salmonella virulence
March 2010 - August 2018
Universidad de Chile
Field of study
Publications
Publications (15)
Type VI Secretion Systems (T6SS), widely distributed in Gram-negative bacteria, contribute to interbacterial competition and pathogenesis through the translocation of effector proteins to target cells. Salmonella harbor 5 pathogenicity islands encoding T6SS (SPI-6, SPI-19, SPI-20, SPI-21 and SPI-22), in which a limited number of effector proteins h...
The type VI secretion system (T6SS) is a contact-dependent contractile multiprotein apparatus widely distributed in Gram-negative bacteria. These systems can deliver different effector proteins into target bacterial and/or eukaryotic cells, contributing to the environmental fitness and virulence of many bacterial pathogens. Salmonella harbors five...
The type VI secretion system (T6SS) is a contact-dependent contractile multiprotein apparatus widely distributed in Gram-negative bacteria. These systems can deliver different effector proteins into target bacterial and/or eukaryotic cells, contributing to the environmental fitness and virulence of many bacterial pathogens. Salmonella harbors five...
Salmonella genus harbors five Type VI Secretion System (T6SS) gene clusters. The T6SS encoded in SPI-6 (T6SSSPI-6) contributes to Salmonella Typhimurium colonization of chickens and mice, while the T6SS encoded in SPI-19 (T6SSSPI-19) of Salmonella Gallinarum contributes to chicken colonization. Interestingly, the T6SSSPI-19 of Salmonella Gallinarum...
The Type VI Secretion System (T6SS) is a multiprotein device that has emerged as an important fitness and virulence factor for many Gram-negative bacteria through the injection of effector proteins into prokaryotic or eukaryotic cells via a contractile mechanism. While some effector proteins specifically target bacterial or eukaryotic cells, others...
The type III secretion systems (T3SS) encoded in pathogenicity islands SPI-1 and SPI-2 are key virulence factors of Salmonella. These systems translocate proteins known as effectors into eukaryotic cells during infection. To characterize the functionality of T3SS effectors, gene fusions to the CyaA’ reporter of Bordetella pertussis are often used....
Acidithiobacillus ferrooxidans resists extremely high concentrations of copper. Strain ATCC 53993 is much more resistant to the metal compared with strain ATCC 23270, possibly due to the presence of a genomic island in the former one. The global response of strain ATCC 53993 to copper was analyzed using iTRAQ (isobaric tag for relative and absolute...
Lipid A is the bioactive component of lipopolysaccharide, and presents a dynamic structure that undergoes modifications in response to environmental signals. Many of these structural modifications influence Salmonella virulence. This is the case of lipid A hydroxylation, a modification catalyzed by the dioxygenase LpxO. Although it has been establi...
Background: Salmonella pathogenicity island (SPI)‐13 is conserved in many serovars of S. enterica, including S. Enteritidis, S. Typhimurium and S. Gallinarum. However, it is absent in typhoid serovars such as S. Typhi and Paratyphi A, which carry SPI‐8 at the same genomic location. Because the interaction with macrophages is a critical step in Salm...
Background:
Vaginal infections are a frequent cause for consultation, but their prevalence and etiology vary in different populations.
Objectives:
To determine the prevalence and etiologies of vaginal infection in women attending a family health center in the Metropolitan Region of Chile.
Methods:
The microbiological diagnosis was made by wet...
Background: Vaginal infections are a frequent cause for consultation, but their prevalence and etiology vary in different populations. Objectives: To determine the prevalence and etiologies of vaginal infection in women attending a family health center in the Metropolitan Region of Chile. Methods: The microbiological diagnosis was made by wet mount...
Questions
Question (1)
The CyaA reporter assays is a classical method to determine if a bacterial effector is translocated to a host cell, commonly epithelial cell lines. In principle, this technique should work too in other eukaryotic cells, but I do not know if it needs modifications to be used in amoebas, such as Dictyostelium or the same commercial kits for cAMP detection are useful.
Thank you a lot for your help.
