Fernanda De Carvalho-Niebel

Fernanda De Carvalho-Niebel
L’Institut national de la recherche agronomique (Toulouse) · Laboratory of Plant-Microbe Interactions (LIPM)

PhD

About

53
Publications
9,180
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4,030
Citations
Citations since 2016
16 Research Items
2036 Citations
2016201720182019202020212022050100150200250300350
2016201720182019202020212022050100150200250300350
2016201720182019202020212022050100150200250300350
2016201720182019202020212022050100150200250300350
Additional affiliations
October 1998 - present
UMR 2594/441 CNRS-INRA
Position
  • CNRS Researcher

Publications

Publications (53)
Article
Full-text available
The cell wall is the primary interface between plant cells and their immediate environment and must balance multiple functionalities, including the regulation of growth, the entry of beneficial microbes, and protection against pathogens. Here, we demonstrate how API, a SCAR2 protein component of the SCAR/WAVE complex, controls the root cell wall ar...
Article
Full-text available
During root nodule symbiosis, intracellular accommodation of rhizobia by legumes is a prerequisite for nitrogen fixation. For many legumes, rhizobial colonization initiates in root hairs through transcellular infection threads. In Medicago truncatula, VAPYRIN (VPY) and a putative E3 ligase LUMPY INFECTIONS (LIN) are required for infection thread de...
Article
Full-text available
The formation of nitrogen-fixing nodules in legumes involves the initiation of synchronized programs in the root epidermis and cortex to allow rhizobial infection and nodule development. In this study, we provide evidence that symplastic communication, regulated by callose turnover at plasmodesmata (PD), is important for coordinating nodule develop...
Article
Full-text available
Spatiotemporal changes in cellular calcium (Ca2+) concentrations are essential for signal transduction in a wide range of plant cellular processes. In legumes, nuclear and perinuclear-localized Ca2+ oscillations have emerged as key signatures preceding downstream symbiotic signaling responses. Förster resonance energy transfer (FRET) yellow-based C...
Article
Full-text available
Medicago truncatula is widely used as a model legume for symbiotic and pathogenic microbial interaction studies. Although a number of Agrobacterium-mediated transformation methods have been developed for M. truncatula, a rapid root transformation system was not yet available for this model plant. Here, we describe an easy method for rapid transgene...
Article
Bacterial accommodation inside living plant cells is restricted to the nitrogen-fixing root nodule symbiosis. In many legumes, bacterial uptake is mediated via tubular structures called infection threads (ITs). To identify plant genes required for successful symbiotic infection, we screened an ethyl methanesulfonate mutagenized population of Lotus...
Article
Full-text available
Legumes improve their mineral nutrition through nitrogen-fixing root nodule symbioses with soil rhizobia. Rhizobial infection of legumes is regulated by a number of transcription factors, including ERF Required for Nodulation 1 (ERN1). Medicago truncatula plants defective in ERN1 are unable to nodulate, but still exhibit early symbiotic responses i...
Article
Full-text available
PUB1, an E3 ubiquitin ligase, which interacts with and is phosphorylated by the LYK3 symbiotic receptor kinase, negatively regulates rhizobial infection and nodulation during the nitrogen-fixing root nodule symbiosis in Medicago truncatula. In this study, we show that PUB1 also interacts with and is phosphorylated by DMI2, the key symbiotic recepto...
Article
Full-text available
Biological nitrogen fixation in legumes occurs in nodules that are initiated in the root cortex following Nod factor recognition at the root surface, and this requires coordination of diverse developmental programs in these different tissues. We show that while early Nod factor signaling associated with calcium oscillations is limited to the root s...
Article
Full-text available
The endosymbiotic association between legumes and soil bacteria called rhizobia leads to the formation of a new root-derived organ called the nodule in which differentiated bacteria convert atmospheric nitrogen into a form that can be assimilated by the host plant. Successful root infection by rhizobia and nodule organogenesis require the activatio...
Article
Full-text available
In many legumes, root entry of symbiotic nitrogen-fixing rhizobia occurs via host-constructed tubular tip-growing structures known as infection threads. Here we have used a confocal microscopy live-tissue imaging approach to investigate early stages of infection thread formation in Medicago truncatula root hairs expressing fluorescent protein fusio...
Article
During endosymbiotic interactions between legume plants and nitrogen-fixing rhizobia, successful root infection by bacteria and nodule organogenesis requires the perception and transduction of bacterial lipo-chitooligosaccharidic signal called Nod Factor (NF). NF perception in legume roots leads to the activation of an early signaling pathway and o...
Article
Full-text available
Rhizobium-induced root nodules are organs specialized for symbiotic nitrogen fixation. Indeterminate-type nodules are formed from an apical meristem and exhibit a spatial zonation which corresponds to successive developmental stages. To get a dynamic and integrated view of plant and bacterial gene expression associated with nodule development, we s...
Article
Full-text available
The availability of next-generation sequences of transcripts from prokaryotic organisms offers the opportunity to design a new generation of automated genome annotation tools not yet available for prokaryotes. In this work, we designed EuGene-P, the first integrative prokaryotic gene finder tool which combines a variety of high-throughput data, inc...
Article
Full-text available
ABSTRACT Rhizobial Nodulation Factors (NF) activate a specific signaling pathway in Medicago truncatula root hairs (RHs) that involves the complex interplay of NSP1/NSP2 GRAS and ERN1 ERF transcription factors (TFs) to achieve full ENOD11 transcription. ERN1 acts as a direct transcriptional regulator of ENOD11 through the activation of the NF-respo...
Article
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Many genes which are associated with root nodule development and activity in the model legume Medicago truncatula have been described. However information on precise stages of activation of these genes and their corresponding transcriptional regulators is often lacking. Whether these regulators are shared with other plant developmental programs als...
Article
Full-text available
LYK3 is a lysin motif receptor-like kinase of Medicago truncatula, which is essential for the establishment of the nitrogen-fixing, root nodule symbiosis with Sinorhizobium meliloti. LYK3 is a putative receptor of S. meliloti Nod factor signals, but little is known of how it is regulated and how it transduces these symbiotic signals. In a screen fo...
Article
Full-text available
Annexins belong to a family of multi-functional membrane- and Ca(2+)-binding proteins. The characteristic feature of these proteins is that they can bind membrane phospholipids in a reversible, Ca(2+)-dependent manner. While animal annexins have been known for a long time and are fairly well characterized, their plant counterparts were discovered o...
Article
Genetic approaches have proved to be extremely useful in dissecting the complex nitrogen-fixing Rhizobium-legume endosymbiotic association. Here we describe a novel Medicago truncatula mutant called api, whose primary phenotype is the blockage of rhizobial infection just prior to nodule primordium invasion, leading to the formation of large infecti...
Chapter
The ability to transform M. truncatula via Agrobacterium tumefaciens was one of the original criteria used to select this legume as a model species (Barker et al., 1990). The Agrobacterium T-DNA transfer machinery generates simple, well-characterized insertion profiles consisting of a small number of T-DNA copies (Tinland, 1996). Conventional A. tu...
Article
Full-text available
Rhizobium Nod factors (NFs) are specific lipochitooligosaccharides that activate host legume signaling pathways essential for initiating the nitrogen-fixing symbiotic association. This study describes the characterization of cis-regulatory elements and trans-interacting factors that regulate NF-dependent and epidermis-specific gene transcription in...
Article
Full-text available
Rhizobial Nod factors are key symbiotic signals responsible for starting the nodulation process in host legume plants. Of the six Medicago truncatula genes controlling a Nod factor signaling pathway, Nod Factor Perception (NFP) was reported as a candidate Nod factor receptor gene. Here, we provide further evidence for this by showing that NFP is a...
Article
Full-text available
The MtENOD11 gene from the model legume Medicago truncatula is transcriptionally activated both in response to Sinorhizobium meliloti Nod factors and throughout infection of root tissues by the nitrogen-fixing microsymbiont. To identify the regulatory sequences involved in symbiosis-related MtENOD11 expression, a series of promoter deletions drivin...
Article
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This article describes an efficient procedure to study Nod factor-induced gene expression in root hairs of the model legume Medicago truncatula. By developing an improved method of fracturing frozen root hairs, it has been possible to obtain a highly purified root hair fraction from M. truncatula seedlings yielding sufficient RNA for real-time quan...
Article
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Four Medicago truncatula sunn mutants displayed shortened roots and hypernodulation under all conditions examined. The mutants, recovered in three independent genetic screens, all contained lesions in a leucine-rich repeat (LRR) receptor kinase. Although the molecular defects among alleles varied, root length and the extent of nodulation were not s...
Article
The efficiency of Agrobacterium tumefaciens transformation of the model legume Medicago truncatula cv. Jemalong (genotype 2HA) was evaluated for strains LBA 4404, C58pMP90, C58pGV2260 and AGL1. Binary vectors carrying promoter- gus/ gfp reporter gene fusions and the nptII gene as selectable marker were used for plant in vitro transformation/regener...
Article
Full-text available
The Medicago truncatula MtAnn1 gene, encoding a putative annexin, is transcriptionally activated in root tissues in response to rhizobial Nod factors. To gain further insight into MtAnn1 function during the early stages of nodulation, we have examined in detail both spatio-temporal gene expression patterns and MtAnn1 activity and localisation in ro...
Article
Here we report the characterization of a new Nod factor-induced gene from Medicago truncatula identified by mRNA differential display. This gene, designated MtAnn1, encodes a protein homologous to the annexin family of calcium- and phospholipid-binding proteins. We further show that the MtAnn1 gene is also induced during symbiotic associations with...
Chapter
Nitrogen fixation in the Rhizobium-legume symbiosis requires the formation of specialized organs, the root nodules. The nodulation process is accompanied by the expression of specific plant genes, designated as nodulin genes. We have recently described the identification, by differential screening of a cDNA nodule library with a subtractive probe,...
Chapter
During nodulation many plant genes are induced or enhanced in expression (in comparison to roots) presumably in order to fulfil roles in the production and functioning of the new organ. These genes have been termed nodulins and have been further classified into late and early types depending on their timing of expression. Initially nodulin clones w...
Article
Full-text available
We report the identification of new molecular markers associated with different stages of Rhizobium-induced nodule development in the legume Medicago truncatula. A cDNA library was constructed from pre-nitrogen-fixing M. Truncatula nodules, and differentially screened with a polymerase chain reaction-amplified subtracted probe. Twenty-nine new fami...
Article
Full-text available
Silencing of a Nicotiana plumbaginifolia beta-1,3-glucanase (gn1) transgene in tobacco line T17 occurs in homozygous and in haploid plants with one transgene locus dosage per chromosome set. We have previously shown that the silent state is manifested by a reduced gn1 steady state mRNA level and results from a post-transcriptional process that is u...
Article
In a hypersensitive reaction to pathogen infection, expression of the beta-1,3-glucanase gn1 gene is induced in cells surrounding the necrotic lesions. The 5'-flanking sequence of gn1 was examined to investigate the molecular basis controlling activation of gene expression during this plant defense response. Studies on transgenic tobacco plants con...
Chapter
Recent studies have demonstrated that introduction of sense gene constructs in plants can result in transgenic plants which express the transgene but also in plants with suppressed levels of transgene expression (reviewed by Jorgensen 1991; Kooter and Mol 1993; Matzke and Matzke 1993). In many silencing cases, the transgene and homologous host gene...
Chapter
The Nicotianaplumbaginifolia gnl gene coding for a basic ß-1,3-glucanase has been constitutively expressed in SR1 Nicotiana tabacum plants. Analysis of the progeny of the transgenic plant with the highest level of gnl expression revealed an interesting phenomenon of gene suppression. Plants hemizygous for the T-DNA locus contained high levels of gn...
Article
Full-text available
A chimeric construct containing the Nicotiana plumbaginifolia beta-1,3-glucanase gn1 gene was introduced into Nicotiana tabacum SR1 to produce high levels of the enzyme constitutively. We determined that the GN1 protein represents a basic beta-1,3-glucanase isoform which accumulates into the vacuoles of the transgenic plants. Analysis of the progen...
Article
A chimeric construct containing the Nicotiana plumbaginifolia fl-1,3-glucanase gnl gene was introduced into Nicotiana tabacum SR1 to produce high levels of the enzyme constitutively. We determined that the GN1 protein represents a basic f-1,3-glucanase isoform which accumulates into the vacuoles of the transgenic plants. Analysis of the progeny of...
Article
Full-text available
The Nicotiana plumbaginifolia gn1 gene encoding a beta-1,3-glucanase isoform has been characterized. The gn1 product represents an isoform distinct from the previously identified tobacco beta-1,3-glucanases. By expressing gn1 in Escherichia coli, we have determined directly that the encoded protein does, indeed, correspond to a beta-1,3-glucanase....
Article
Full-text available
The Nicotiana plumbaginifolia gn1 gene encoding a β-1,3-glucanase isoform has been characterized. The gn1 product represents an isoform distinct from the previously identified tobacco β-1,3-glucanases. By expressing gn1 in Escherichia coli, we have determined directly that the encoded protein does, indeed, correspond to a β-1,3-glucanase. In N. plu...

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