Feng Cai

Feng Cai
Sun Yat-Sen University | SYSU · School of Ecology

PhD

About

50
Publications
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Publications

Publications (50)
Chapter
Before the introduction of molecular techniques, the taxonomy of Trichoderma consisted of several dozen species defined based on their morphology, biogeography, and ecophysiology. However, the last two decades have resulted in the rapid expansion of species diversity in the genus. Currently, the genus Trichoderma has at least 469 species names, amo...
Chapter
The spectrum of filamentous fungi emerging as opportunistic human pathogens is widening constantly. Various types of mycoses with often severe, occasionally even fatal outcome have been attributed to different species of the genus Trichoderma, usually in patients with an impaired immune system. However, cases involving non-immunocompromised hosts a...
Chapter
Mycoparasitism of Trichoderma (Hypocreales, Ascomycota) on plant pathogenic fungi is used for the biological control of such pests. To develop next-generation technology-driven Trichoderma-based products, recent studies have addressed the molecular mechanisms of Trichoderma mycoparasitism. In this chapter, we review the studies of functional geneti...
Chapter
This chapter presents a literature overview about the green mould disease of cultivated mushrooms caused by Trichoderma species, a problem which may result in serious economic damage due to substantial losses of crop yield in mushroom production. The first major green mould epidemic struck the production of white button mushroom (Agaricus bisporus)...
Article
Pythium species are devasting pathogens causing major crop losses, e.g., damping-off in sugar beet caused by Pythium ultimum and root-rot of tomato caused by Pythium aphanidermatum. The use of natural antagonistic microorganisms is a promising environment-friendly approach to control Pythium-caused plant diseases. There are several examples of bioc...
Article
Asexually reproducing fungi play a significant role in essential processes in managed and wild ecosystems such as nutrients cycling and multitrophic interactions. A large number of such taxa are among the most notorious plant and animal pathogens. In addition, they have a key role in food production, biotechnology and medicine. Taxa without or rare...
Article
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Comparative and pan-genomic analyses of the endophytic fungus Pezicula neosporulosa (Helotiales, Ascomycota) from needles of the relict fir, Abies beshanzuensis, showed expansions of carbohydrate metabolism and secondary metabolite biosynthetic genes characteristic for unrelated plant-beneficial helotialean, such as dark septate endophytes and eric...
Article
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Higher fungi can rapidly produce large numbers of spores suitable for aerial dispersal. The efficiency of the dispersal and spore resilience to abiotic stresses correlate with their hydrophobicity provided by the unique amphiphilic and superior surface-active proteins–hydrophobins (HFBs)–that self-assemble at hydrophobic/hydrophilic interfaces and...
Article
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Conidia of Trichoderma guizhouense (Hypocreales, Ascomycota) are frequently applied to the production of biofertilizers and biocontrol agents. Conidiation of some Trichoderma species depends on blue light and the action of different blue light receptors. However, the interplay between different blue‐light receptors in light signaling remained elusi...
Article
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Understanding how organisms adapt to extreme living conditions is central to evolutionary biology. Dark septate endophytes (DSEs) constitute an important component of the root mycobiome and they are often able to alleviate host abiotic stresses. Here, we investigated the molecular mechanisms underlying the beneficial association between the DSE Lab...
Article
In this review, we argue that there is much to be learned by transferring knowledge from research on lignocellulose degradation to that on plastic. Plastic waste accumulates in the environment to hazardous levels, because it is inherently recalcitrant to biological degradation. Plants evolved lignocellulose to be resistant to degradation, but with...
Article
The ascomycetous filamentous fungal genus Trichoderma has at least two faces, like Agni, the God of fire in Hindu mythology. Or maybe even three? The ‘Good’, the ‘Bad’ and the ‘Ugly’? In fact, Trichoderma species can be considered: (1) ‘Good’—as plant-beneficial microorganisms in agriculture due to a plethora of antagonistic mechanisms acting again...
Article
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Arsenic can be biomethylated to form a variety of organic arsenicals differing in toxicity and environmental mobility. Trivalent methylarsenite (MAs(III)) produced in the methylation process is more toxic than inorganic arsenite (As(III)). MAs(III) also serves as a primitive antibiotic and, consequently, some environmental microorganisms have evolv...
Article
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Modern taxonomy has developed towards the establishment of global authoritative lists of species that assume the standardized principles of species recognition, at least in a given taxonomic group. However, in fungi, species delimitation is frequently subjective because it depends on the choice of a species concept and the criteria selected by a ta...
Article
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The secretomes of filamentous fungi contain a diversity of small secreted cysteine-rich proteins (SSCPs) that have a variety of properties ranging from toxicity to surface activity. Some SSCPs are recognized by other organisms as indicators of fungal presence, but their function in fungi is not fully understood. We detected a new family of fungal s...
Chapter
Fungi comprise one of the most diverse groups of eukaryotes with many cryptic species that are difficult to identify. In this chapter, we detail a protocol for the molecular identification of the most industrially relevant species of Trichoderma ,T. reesei. We first describe how a single spore culture should be isolated and used for the sequencing of...
Chapter
The filamentous fungus Trichoderma reesei (Hypocreales, Ascomycota) is an efficient industrial cell factory for the production of cellulolytic enzymes used for biofuel and other applications. Therefore, researches addressing T. reesei are relatively advanced compared to other Trichoderma spp. because of the significant bulk of available knowledge,...
Chapter
The excellent abilities of cellulase production for biofuel and other industries and the plant-beneficial potential of Trichoderma spp. have led this fungus to being subjected to intensive genetic engineering and become one of the iconic examples for fungal genetics. Genetic manipulation to improve the strains is a highly effective means of meeting...
Article
The production of biofuels from plant biomass is dependent on the availability of enzymes that can hydrolyze the plant cell wall polysaccharides to their monosaccharides. These enzyme mixtures are formed by microorganisms but their native compositions and properties are often not ideal for application. Genetic engineering of these microorganisms is...
Article
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The website http://outlineoffungi.org, is launched to provide a continuous up-to-date classification of the kingdom Fungi (including fossil fungi) and fungus-like taxa. This is based on 1516 recent publications and on the outline of fungi and fungus-like taxa published recently (Mycosphere 11, 1060-1456, Doi: 10.5943/mycosphere/11/1/8). The website...
Preprint
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Fungi can rapidly produce large amounts of spores suitable for aerial dispersal. The hydrophobicity of spores is provided by the unique amphiphilic and superior surface-active proteins - hydrophobins (HFBs) - that self-assemble at hydrophobic/hydrophilic interfaces and thus change surface properties. Using the HFB-enriched mold Trichoderma and the...
Article
Full-text available
Fungal evolutionary biology is impeded by the scarcity of fossils, irregular life cycles, immortality, and frequent asexual reproduction. Simple and diminutive bodies of fungi develop inside a substrate and have exceptional metabolic and ecological plasticity, which hinders species delimitation. However, the unique fungal traits can shed light on e...
Article
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Aims: Sustainable agriculture requires effective and safe biofertilizers and biofungicides with low environmental impact. Natural ecosystems that closely resemble the conditions of biosaline agriculture may present a reservoir for fungal strains that can be used as novel bioeffectors. Methods and results: We isolated a library of fungi from the...
Article
Cerato-platanins (CPs) are surface-active small proteins abundantly secreted by filamentous fungi. Consequently, immune systems of plants and other organisms recognize CPs and activate defense mechanisms. Some CPs are toxic to plants and act as virulence factors in plant-pathogenic fungi. Our analysis, however, demonstrates that the interactions wi...
Article
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(1) Background: Mechanisms of cellular and molecular adaptation of fungi to salinity have been commonly drawn from halotolerant strains and few studies in basidiomycete fungi. These studies have been conducted in settings where cells are subjected to stress, either hypo- or hyperosmotic, which can be a confounding factor in describing physiological...
Article
Trichoderma species are abundant in different agricultural habitats, but some representatives of this genus, mainly clade Longibrachiatum members are also emerging as causative agents of various human diseases with even fatal outcome. Strains of these species frequently show resistance to commonly used azole antifungals. Based on previous data it i...
Article
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Abstract Survival of inoculated fungal strains in a new environment plays a critical role in functional performance, but few studies have focused on strain-specific quantitative PCR (qPCR) methods for monitoring beneficial fungi. In this study, the Trichoderma guizhouense strain NJAU 4742 (transformed with the gfp gene and named gfp-NJAU 4742), whi...
Article
Coastal saline soil is an important reserve land resource that has high potential for agricultural utilization. The present study adopted a high-throughput absolute quantification 16S rRNA sequencing method to investigate the effect of four different fertilization regimes (namely 100% of bio-organic fertilizer, 70% of bio-organic fertilizer +30% of...
Article
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Background: The growing importance of the ubiquitous fungal genus Trichoderma (Hypocreales, Ascomycota) requires understanding of its biology and evolution. Many Trichoderma species are used as biofertilizers and biofungicides and T. reesei is the model organism for industrial production of cellulolytic enzymes. In addition, some highly opportunis...
Article
Full-text available
When resources are limited, the hypocrealean fungus Trichoderma guizhouense can overgrow another hypocrealean fungus Fusarium oxysporum, cause sporadic cell death and arrest growth. A transcriptomic analysis of this interaction shows that T. guizhouense undergoes a succession of metabolic stresses while F. oxysporum responded relatively neutrally b...
Article
Full-text available
Unlike most other fungi, molds of the genus Trichoderma (Hypocreales, Ascomycota) are aggressive parasites of other fungi and efficient decomposers of plant biomass. Although nutritional shifts are common among hypocrealean fungi, there are no examples of such broad substrate versatility as that observed in Trichoderma. A phylogenomic analysis of 2...
Data
Composition and evolution of pcwdCAZome of Trichoderma and related fungi. A. NCBI Accession numbers of genes composing the pcwdCAZome of Trichoderma and regulatory proteins analyzed in this study. B. Distribution of pcwdCAZymes in GH families in Hypocreales. C: Parameters of phylogenetic analyses of individual proteins from Trichoderma pcwdCAZome....
Data
Additional organisms used in this study. (XLSX)
Data
Phylogenetic trees of Trichoderma plant cell wall-degrading carbohydrate active enzymes and regulatory proteins. (PDF)
Data
Results of statistical tests of the LGT hypothesis. A. The transfer costs for NOTUNG and comparison to LGT events predicted by T-REX. B: Results of the NOTUNG analysis of pcwdCAZome and relevant regulatory proteins. C. Summary on the evolutionary origin of pcwdCAZome of Trichoderma inferred in this study. D. Functional annotations of core genome Tr...
Data
Growth of Trichoderma spp., Escovopsis weberi and Pestalotiopsis fici on natural substrates resembling polymers in the fungal and plant cell walls. Strains were evaluated after 10 days of incubation at 28°C in darkness. Yellow, green and white shape outlines correspond to good, weak and no growth, respectively. Data are representatives of four sepa...
Data
Multiple sequence alignment used for the multilocus phylogeny of Ascomycota fungi. (AA)
Data
Accession numbers of genes used for the multilocus phylogeny of Ascomycota fungi. (XLSX)
Data
Mycoparasitism of Trichoderma. A. Allomycoparasitism of Trichoderma spp. and E. weberi on Lentinula edodes. B: Allomycoparasitism of Trichoderma and E. weberi on Leucoagaricus gongylophorus. The dashed lines indicate growth of the host fungus as deduced from back sides of the plates. C: Set up for the microscopic investigation of Trichoderma (right...
Data
Ecological terminology used in this study to describe types of nutrition found in Hypocreales fungi. (PDF)
Data
Properties of fungal genomes that were used in this study. (XLSX)
Data
Materials describing phylogenomic analysis of Hypocreales based on 100 orthologous proteins. A. Annotations of 100 orthologous proteins used in phylogenomic analysis and results of the neutrality tests. B. Protein accession numbers for 23 genomes. (XLSX)
Data
Chromosomal location of individual genes from pcwdCAZome of T. reesei. (XLSX)
Article
Full-text available
Understanding the responses of soil microbiome composition to various farming practices is important for selecting suitable managements to maintain soil functions. In this study, the influences of heavy chemical fertilizer application (CF) and reduced chemical fertilizer supplemented with organic (OF) or bioorganic fertilizer (BF, BF = OF + Trichod...
Article
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AimsInvestigating shifts in soil microbiomes driven by different fertilization regimes would be helpful for lessening the negative effect of monoculture in agricultural systems. Methods In the present work, we employed MiSeq sequencing to evaluate the response of local microbial communities to three different fertilization regimes, i.e., heavy chem...
Article
Hydrophobins are small secreted cysteine-rich proteins exclusively found in fungi. They are able to self-assemble in single molecular layers at hydrophobic-hydrophilic interfaces and can therefore be directly involved in establishment of fungi in their habitat. The genomes of filamentous mycotrophic fungi Trichoderma encode a rich diversity of hydr...
Article
Many studies in recent decades have shown the signature effect of the host plant in determining the plant-associated microbiome in the soil. However, the important question as to the factors contributing to the selective enrichment of microorganisms in the plant rhizosphere has not been fully addressed. In this study, the role of the nutrient prefe...
Article
Full-text available
Trichoderma harzianum strain SQR-T037 is a biocontrol agent that has been shown to enhance the uptake of nutrients (macro- and microelements) by plants in fields. The objective of this study was to investigate the contribution of SQR-T037 to P and microelement (Fe, Mn, Cu and Zn) nutrition in tomato plants grown in soil and in hydroponic conditions...
Article
Full-text available
Aims This research was to study the capacity of Trichoderma harzianum strain SQR-T037 to colonize tomato roots and how this strain and its bio-organic fertilizer stimulate plant growth and increase yields under field conditions. Methods Field trials were conducted with a reduced application of chemical fertilizer (75 % of the recommended applicatio...
Article
A detailed understanding of the effect of natural products on plant growth and protection will underpin new product development for plant production. The isolation and characterization of a known secondary metabolite named harzianolide from Trichoderma harzianum strain SQR-T037 were described, and the bioactivity of the purified compound as well as...

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Project (1)
Project
Functional genetics of surface-active proteins hydrophobins (HFBs) in Trichoderma and their relationships with its root colonization