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Publications (29)
Biosensors based on miniaturized, functional electrodes are of high potential for various biosensing applications, especially at the point-of-care setting among others. However, the sensor performance of such electrochemical devices is still strongly limited, especially due to surface fouling in complex sample fluids, such as blood serum. Electrode...
Tissue engineering is rapidly progressing toward clinical application. In the musculoskeletal field, there has been an increasing necessity for bone and cartilage replacement. Despite the promising translational potential of tissue engineering approaches, careful attention should be given to the quality of developed constructs to increase the real...
The Lateral Flow Immuno Assay (LFIA) is a well-established technique that provides immediate results without high-cost laboratory equipment and technical skills from the users. However, conventional colorimetric LFIA strips suffer from high limits of detection, mainly due to the analysis of a limited sample volume, short reaction time between the t...
Mitochondrial function is central to human health and has been recently shown to be modulated by magnetic field‐induced TRPC1 (red) activation. In article number 2000146, Petra S. Dittrich, Alfredo Franco‐Obregón, and co‐workers show that cell‐derived vesicles from wild type muscle are capable of reinstating lost mitochondrial sensitivity to magnet...
Mitochondrial function is central to human health and has been recently shown to be modulated by magnetic field‐induced TRPC1 (red) activation. In article number 2000146, Petra S. Dittrich, Alfredo Franco‐Obregón, and co‐workers show that cell‐derived vesicles from wild type muscle are capable of reinstating lost mitochondrial sensitivity to magnet...
Glucose sensors are potentially useful tools for monitoring the glucose concentration in cell culture medium. Here, we present a new, low-cost, and reproducible sensor based on a cellulose-based material, 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) oxidized-cellulose nanocrystals (CNCs). This novel biocompatible and inert nanomaterial is employed...
Pulsed electromagnetic fields (PEMFs) are capable of specifically activating a TRPC1‐mitochondrial axis underlying cell expansion and mitohormetic survival adaptations. This study characterizes cell‐derived vesicles (CDVs) generated from C2C12 murine myoblasts and shows that they are equipped with the sufficient molecular machinery to confer mitoch...
Pulsed electromagnetic fields (PEMFs) are capable of specifically activating a TRPC1- mitochondrial axis underlying cell expansion and mitohormetic survival adaptations. This study characterizes cell-derived vesicles (CDVs) generated from C2C12 murine myoblasts and shows that they are equipped with the sufficient molecular machinery to confer mitoc...
In vitro models, cell culture models, microtissue, organ-on-a-chip, microfabrication, micropumps, membrane, manufacturing, filtration, vascularization, perfusion, microfluidics, channels, cleanroom, silicon, glass, polydimethylsiloxane, additive manufacturing, bioprinting, bioprinter, three-dimensional printing, bioreactor, stimulation, biointerfac...
Oligo‐arginines are thoroughly studied cell‐penetrating peptides (CPPs, Figs. 1, 2). Previous in‐vitro investigations with the octaarginine salt of the phosphonate fosmidomycin (herbicide and anti‐malaria drug) have shown a 40‐fold parasitaemia inhibition with P. falciparum, compared to fosmidomycin alone (Fig. 3). – We have now tested this salt, a...
A quantitative analysis by confocal fluorescence microscopy of the entry into HEK293 and MCF‐7 cells by fluorescein‐labeled octaarginine (1) and by three octa‐Adp derivatives (2‐4, octamers of the β‐Asp‐Arg‐dipeptide, derived from the biopolymer cyanophycin) is described, including the effects of the membrane dye R18 and of DMSO on cell penetration...
We present a microfluidic strategy for the detection of botulinum neurotoxin (BoNT) physiological potency using nerve cell-mimicking liposomes. The test, optimized for BoNT type A (BoNT/A), displays all physiological key activities, namely specific receptor binding, translocation of the active site via the lipid bilayer, and targeted proteolytic cl...
The development of efficacious anticancer therapeutics is difficult due to the heterogeneity of the cellular response to chemotherapy. Anticancer peptides (ACPs) are promising drug candidates that have been shown active against a range of cancer cells. However, few ACP studies focus on the tumour single-cell heterogeneities. In order to address thi...
Microfluidic devices capable of manipulating and guiding small fluid volumes open new methodical approaches in the fields of biology, pharmacy, and medicine. They have already proven their extraordinary value for cell analysis. The emergence of microfluidic platforms has paved the way to novel analytical strategies for the positioning, treatment an...
The developmental sensitivity of skeletal muscle to mechanical forces is unparalleled in other tissues. Calcium entry via reputedly mechanosensitive transient receptor potential (TRP) channel classes has been shown to play an essential role in both the early proliferative stage and subsequent differentiation of skeletal muscle myoblasts, particular...
Individual bacteria of an isogenic population can differ significantly in their phenotypic characteristics. This cellular heterogeneity is thought to increase the adaptivity to environmental changes on a population level. Analytical methods for single-bacteria analyses are essential to reveal the different factors that may contribute to this cellul...
Here we present a stage perfusion incubation system that allows for the cultivation of mammalian cells within PDMS microfluidic devices for long-term microscopic examination and analysis. The custom-built stage perfusion incubator is adaptable to any x-y microscope stage and is enabled for temperature, gas and humidity control as well as equipped w...
We present a novel analytical strategy for the measurement of membrane channel dynamics involved in cellular mechanotransduction signaling pathways. It consists of a conventional PDMS microfluidic device for the culture and mechanical actuation of C2C12 murine myoblasts. Herein, fluid-flow induced shear forces stimulate stretch-activated channels (...
We present a novel strategy to enable cellular analysis of in vivo models in microfluidic devices. The strategy combines microdissection of tissue samples and microfluidics for cell capturing and analysis. The technique is optimized for cell capture and analysis of bone, so spatial information can be linked to important information concerning indiv...
We present a microfluidic device that enables parallel single E. coli trapping and isolation in microchambers with an extremely small volume of 80 pL. Subsequent bacteria lysis and analysis by an immunological assay allowed reliable measurement of the β-galactosidase content of single E. coli bacteria.
Fueled by technological advances in micromanipulation methodologies, the field of mechanobiology has boomed in the last decade. Increasing needs for clinical solutions to better maintain our major mechanosensitive tissues (muscle, bone, and cartilage) with increasing age and new insights into cellular adaptations to mechanical stresses beckon for n...
Adenosine phosphate and NAD cofactors play a vital role in the operation of cell metabolism, and their levels and ratios are
carefully regulated in tight ranges. Perturbations of the consumption of these metabolites might have a great impact on cell
metabolism and physiology. Here, we investigated the impact of increased ATP hydrolysis and NADH oxi...
The combination of a single cell analysis platform with confocal microscopy enabled the monitoring of protein secretion rates directly downstream of a cell.
A bilayer microfluidic chip is used, in which multiple laminar streams are generated to define local microenvironments. The bilayer architecture of the microchip separates cell handling and positioning from cell activation by soluble chemicals. Cell activation is diffusion controlled through a porous membrane. By employing time-lapse fluorescence m...