About
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Introduction
I am currently working in the blueberry breeding laboratory at the University of Florida. I am a plant geneticist with an emphasis on food crops, supporting breeding programs by implementing genome editing and tissue culture approaches.
Current institution
Additional affiliations
July 2019 - February 2020
Position
- Research Assistant
Description
- While I was at MSU, I was involved in a genome editing project that targeted genes related to potatoes' self-incompatibility. I cloned genes using RACE, designed guide RNAs, generated constructs carrying CRISPR-Cas9, and performed Agrobacterium-mediated transformation in a self-incompatible diploid potato line. Since then, I have been supporting different genome editing projects at the Douches potato lab in MSU.
July 2006 - July 2014
Colombian Corporation for Agricultural Research (Agrosavia)
Position
- Researcher
Description
- While I was working at Agrosavia, I was involved in the molecular characterization of native Andean germplasm collections such as cape gooseberry (P. peruviana), lulos (S. quitoense) and tree tomato (S. bataceum). I was particularly interested in understanding the molecular response of cape gooseberry when challenged with one of its major constraints, F. oxysporum. I was also involved in the genetic characterization of important Colombian crops such as palm trees and potatoes.
Education
August 2014 - May 2019
August 2009 - May 2011
February 2001 - June 2006
Publications
Publications (45)
A robust Genotyping-By-Sequencing (GBS) pipeline platform was examined to provide accurate discovery of Single Nucleotide Polymorphisms (SNPs) in a cape gooseberry (Physalis peruviana L.) and related taxa germplasm collection. A total of 176 accessions representing, wild, weedy, and commercial cultivars as well as related taxa from the Colombian ge...
Genetic diversity in plant breeding is key to dissecting the genetic architecture of complex economical traits. Genome-wide association studies (GWAS) and genomic selection (GS) have emerged as promising tools to accelerate agronomic trait improvement efficiency, defining current breeding strategies. The key objective of this study was to investiga...
The Gametophytic Self-Incompatibility (GSI) system in diploid potato (Solanum tuberosum L.) poses a substantial barrier in diploid potato breeding by hindering the generation of inbred lines. One solution is gene editing to generate self-compatible diploid potatoes which will allow for the generation of elite inbred lines with fixed favorable allel...
![Phenotypic (ry\documentclass[12pt]{minimal} \usepackage{amsmath}...](publication/365555124/figure/fig2/AS:11431281108742330@1671592056561/Phenotypic-rydocumentclass12ptminimal-usepackageamsmath-usepackagewasysym_Q320.jpg)
Unlabelled:
Oil palm is the most important oil crop worldwide. Colombia is the fourth largest producer, primarily relying on production from interspecific hybrids, derived from crosses between Elaeis oleifera and Elaeis guineensis (OxG). However, conventional breeding can take up to 20 years to generate a new variety. Therefore, reducing the breed...
The present invention relates to methods for controlling hybridization in plants and producing hybrid plants. The present invention also relates to nucleic acids encoding amino acid sequences for self-incompatibility (SI) proteins in plants, and the use thereof for the manipulation of SI, including seed production, in plants, particularly of the So...
Conventional breeding and genetics in a vegetatively propagated, autotetraploid, highly heterozygous recalcitrant crop species such as potato is challenging. Diploid inbred lines in potato will serve as an invaluable source of material for breeding and functional genetics. Most of the available diploid germplasm in potato is either self-incompatibl...
A diploid potato (Solanum tuberosum L.) recombinant inbred line population was derived from a cross between Solanum chacoense inbred line M6 and S. chacoense accession USDA8380‐1 (80‐1) to identify loci associated with self‐compatibility and Colorado potato beetle resistance. Individuals from the F4 and F5 generations were genotyped on the Illumina...
Vascular wilt, caused by the pathogen Fusarium oxysporum f. sp. physali ( Foph ), is a major disease of cape gooseberry ( Physalis peruviana L.) in Andean countries. Despite the economic losses caused by this disease, there are few studies related to molecular mechanisms in the P. peruviana — Foph pathosystem as a useful tool for crop improvement....
Most wild and cultivated diploid potato species are self-incompatible, preventing inbreeding and promoting outcrossing. To generate diploid inbred lines, the tuber bearing self-compatible species Solanum chacoense (M6 line) has been used to overcome self-incompatibility. In this study, self-compatibility was introgressed from a set of four diploid...
The year 2020 marks a decade since the first gene-edited plants were generated using homing endonucleases and zinc finger nucleases. The advent of CRISPR/Cas9 for gene-editing in 2012 was a major science breakthrough that revolutionized both basic and applied research in various organisms including plants and consequently honored with “The Nobel Pr...
Cape gooseberry (Physalis peruviana L.) is a berry-bearing Solanaceae species, native to the Andean region with enormous potential for biomedical research and commercial purposes. Despite its importance, the species still lacks a more comprehensive platform for Single Nucleotide Polymorphisms (SNPs) discovery. Here we evaluated a total of 176 acces...
Late blight, caused by the oomycete Phytophthora infestans (Mont) de Bary, is the most important disease of cultivated potato (Solanum tuberosum L.). An efficient long-term strategy for controlling late blight infection involves integration of host plant resistance to disease management programs. In this study, a diploid potato population segregati...
Genome editing has revolutionized crop improvement. For a heterozygous tetraploid and vegetatively propagated crop such as cultivated potato, Solanum tuberosum ssp. tuberosum L. (2 n = 4 x = 48), gene editing presents tremendous opportunities for trait improvement. In potato, traits such as improving cold storage and processing, herbicide tolerance...
Background:
The genus Elaeis has two species of economic importance for the oil palm agroindustry: Elaeis oleifera (O), native to the Americas, and Elaeis guineensis (G), native to Africa. This work provides to our knowledge, the first association mapping study in an interspecific OxG oil palm population, which shows tolerance to pests and disease...
Background The genus Elaeis has two species of economic importance for the oil palm agroindustry: Elaeis oleifera (O), native to the Americas, and Elaeis guineensis (G), native to Africa. This work provides to our knowledge, the first association mapping study in an interspecific OxG oil palm population, which shows tolerance to pests and diseases,...
Background The genus Elaeis has two species of economic importance for the oil palm agroindustry: Elaeis oleifera (O), native to the Americas, and Elaeis guineensis (G), native to Africa. This work provides to our knowledge, the first association mapping study in an interspecific OxG oil palm population, which shows tolerance to pests and diseases,...
Background The genus Elaeis has two species of economic importance for the oil palm agroindustry: Elaeis oleifera (O), native to the Americas, and Elaeis guineensis (G), native to Africa. The work presented herein provides, to our knowledge, the first association mapping study in an interspecific OxG hybrid population of oil palm which presents tol...
Background The genus Elaeis has two species of economic importance for the oil palm agroindustry: Elaeis oleifera (O), native to the Americas, and Elaeis guineensis (G), native to Africa. The breeding program in Colombia relies on interspecific OxG crossing populations with tolerance to pests and diseases, high oil quality, and acceptable fruit bun...
Potato breeding can be redirected to a diploid inbred/F1 hybrid variety breeding strategy if self-compatibility can be introduced into diploid germplasm. However, the majority of diploid potato clones (Solanum spp.) possess gametophytic self-incompatibility that is primarily controlled by a single multiallelic locus called the S-locus which is comp...
Fruit set and seed count upon self-pollination in DRH-195 wild-type and S-RNase-derived KO lines.
Retrieved Illumina reads for genomic assembly of the DRH potato lines and assembly statistics.
Primers and sgRNAs used in this study.
Fruit set and seed count upon self-pollination in DRH-310 wild-type and S-RNase-derived KO lines.
S-RNase open reading-frames in T0 knockout (KO) lines derived in two SI diploid potato lines. Sequences shown within the dotted lines contain 5′ and 3′ mRNA borders of this gene. Primer sequences designed to amplify the S-RNase ORF are shown as well as the sgRNA 1 located in exon 1. Individual dots represent nucleotides within the S-RNase gene. All...
Top TBLASTN hits of reported S-RNase proteins in the DM genome assembly.
Potato (Solanum tuberosum) is a staple food crop and is considered one of the main sources of carbohydrates worldwide. Late blight (Phytophthora infestans) and common scab (Streptomyces scabies) are two of the primary production constraints faced by potato farming. Previous studies have identified a few resistance genes for both late blight and com...
Vascular wilt caused by Fusarium oxysporum is the most important disease in cape gooseberry (Physalis peruviana L.) in Colombia. The development of resistant cultivars is considered one of the most cost-effective means to reduce the impact of this disease. In order to do so, it is necessary to provide breeders with molecular markers and promising g...
The genus Physalis is common in the Americas and includes several economically important species, among them is Physalis peruviana that produces appetizing edible fruits. We studied the genetic diversity and population structure of P. peruviana and characterized 47 accessions of this species along with 13 accessions of related taxa consisting of 22...
RESUMEN
La uchuva, Physalis peruviana, es un frutal andino de importancia para la exportación; el principal limitante de su producción en Colombia es el marchitamiento vascular ocasionado por Fusarium oxysporum. En el presente trabajo se propuso generar poblaciones F1 entre parentales contrastantes por su respuesta a éste patógeno y evaluarlas mole...
Colombia is one of the countries with the largest agrobiodiversity in the world. Assessing natural variation of native and adapted germplasm to contribute to biotic and
abiotic stresses tolerance is a major endeavor of the Colombian Corporation for Agricultural Research. Two pilot studies were conducted to learn lessons on the use of
current genoty...
The Cape gooseberry (Physalisperuviana L) is an Andean exotic fruit with high nutritional value and appealing medicinal properties. However, its cultivation faces important phytosanitary problems mainly due to pathogens like Fusarium oxysporum, Cercosporaphysalidis and Alternaria spp. Here we used the Cape gooseberry foliar transcriptome to search...
Severity scale of symptoms for the Physalisperuviana - Fusariumoxysyporum pathosystem.
(DOCX)
Primers designed for the identification of immunity related genes in Cape gooseberry.
(DOCX)
Number of reads per marker sequenced by 454 on the 14 Physalis genotypes.
(DOCX)
La papa es un producto básico de la canasta familiar colombiana y a nivel mundial ocupa el quinto lugar en cuanto a consumo humano y el cuarto lugar en cuanto al valor de la producción, después del maíz, el trigo y el arroz; es el producto de origen agrícola de mayor consumo per cápita en Colombia, con un promedio 69.9 Kg/habitante/año. Dicho
consu...
Evaluación de resistencia o tolerancia de germoplasma de uchuva (Physalis peruviana) a Fusarium oxysporum como contribución al control integrado de la enfermedad. OBJETIVO Evaluar la resistencia o tolerancia al marchitamiento producido por Fusarium oxysporum en Physalis peruviana L como insumo para la generación de variedades resistentes que contri...
Consensus tree using bootstrapping of 10,000 replicates for lulo.
Consensus tree using bootstrapping of 10,000 replicates for tree tomato.
COSII Markers selected for screening.
Genetic distances for tree tomato and relatives.
The Lulo or naranjilla (Solanum quitoense Lam.) and the tree tomato or tamarillo (Solanum betaceum Cav. Sendt.) are both Andean tropical fruit species with high nutritional value and the potential for becoming premium products in local and export markets. Herein, we present a report on the genetic characterization of 62 accessions of lulos (n = 32)...
El lulo (Solanum quitoense) y el tomate de árbol (Solanum betaceum) son frutales andinos con potencial en mercados nacionales y de exportación (Fig. 1). Estas especies representan alternativas productivas para agricultores por su valor nutritivo y pueden ser utilizadas para la conservación y regeneración de ecosistemas de ladera. Peso a esto, su ad...
Physalis peruviana, commonly known as capegooseberry, is an Andean fruited species of the solanaceae family important for local and export markets due to nutritional and medicinal values. Capegooseberry is considered an orphan species with little genetic and genomic information in agreement with the lack of genetic improved materials. The main trai...
