About
11
Publications
1,261
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105
Citations
Current institution
Additional affiliations
March 2021 - July 2024
Hong Kong Center for Cardiovascular and Cerebrovascular Health Engineering Research
Position
- Postdoctoral researcher
Education
September 2016 - February 2021
City University of Hong Kong
Field of study
- Biomedical Engineering
September 2013 - June 2016
September 2011 - May 2013
Publications
Publications (11)
This paper proposes a practical micropipette-image (2-D pixel and 3-D spatial coordination) calibration method indispensable to somatic cell microinjection, leveraging advancements in modern motorized micromanipulators. The method determines the depth information of the micropipette in the microscope field by assessing the contact between the micro...
The efficient application of the newly developed gene-editing method CRISPR/Cas9 requires more accurate intracellular gene delivery. Traditional delivery approaches, such as lipotransfection and non-viral delivery methods, must contend with major problems to overcome the drawbacks of low efficiency, high toxicity, and cell-type dependency. The high...
Despite recent advances in data-independent and deep-learning algorithms, unstained live adherent cell instance segmentation remains a long-standing challenge in cell image processing. Adherent cells' inherent visual characteristics, such as low contrast structures, fading edges, and irregular morphology, have made it difficult to distinguish from...
Many virus-mediated and chemical-based methods for delivering foreign genes into target cells, such as recombinant lentivirus transfection and cationic lipid transfection, are remarkably challenging to use on immune cells because of low efficiency and high toxicity. Microinjection is a promising method to deliver foreign gene expression plasmids in...
The non-viral delivery of the prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) nuclease system provides promising solutions for gene therapy. However, traditional chemical and physical delivery approaches for gene knock-in are confronted by significant challenges to overcome the drawbacks of...
Despite the rapid development in providing precise delivery of extraneous samples to the vast majority of cells, robotic microinjection is still hindered by cumbersome operations and low throughput in practice. This study presents a new automated microinjection system equipped with two micromanipulators and a deep learning algorithm for cell identi...
Introduction of a gene to mesenchymal stem cells (MSCs) is a well-known strategy to purposely manipulate the cell fate and further enhance therapeutic performance in cell-based therapy. Viral and chemical approaches for gene delivery interfere with differentiation potential. Although microinjection as a physical delivery method is commonly used for...
Four-dimensional cone-beam computed tomography (4D CBCT) has been developed to provide a sequence of phase-resolved reconstructions in image-guided radiation therapy. However, 4D CBCT images are degraded by severe streaking artifacts and noise because the phase-resolved image is an extremely sparse-view CT procedure wherein a few under-sampled proj...
Automated microinjection systems for suspension cells have been studied for years. Nevertheless, microinjection systems for adherent cells still suffer from laborious manual operations and low productivity. This paper presents a new automated microinjection system with high productivity for adherent cells. This system enhances productivity through...
