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Publications (89)
Background
Extracellular matrix (ECM) stiffness is closely related to the progress of diabetic cardiomyopathy (DCM) and the response of treatment of DCM to anti-diabetic drugs. Dapagliflozin (Dapa) has been proven to have cardio-protective efficacy for diabetes and listed as the first-line drug to treat heart failure. But the regulatory relationshi...
Ischemic stroke and reperfusion injury result in neuronal damage and dysfunction associated with oxidative stress, leading to overproduction of cellular reactive oxygen species (ROS) and reactive nitrogen species (RNS). In situ monitoring of the transient ROS and RNS effluxes during rapid pathologic processes is crucial for understanding the relati...
Microglia play a crucial role in maintaining the homeostasis of the central nervous system (CNS) by sensing and responding to mechanical and inflammatory cues in their microenvironment. However, the interplay between mechanical and inflammatory cues in regulating microglia activation remains elusive. In this work, we constructed in vitro mechanical...
Due to the inherent disorder and fluidity of water, precise machining of water through laser cutting are challenging. Herein we report a strategy that realizes the laser cutting machining of water through constructing hydrophobic silica nanoparticle-encased water pancakes with sub-millimeter depth. Through theoretical analysis, numerical simulation...
Protein analysis is an important approach for disease diagnosis, in which sample pretreatment is an essential step since protein samples are often complex and many protein biomarkers are of low abundance. Here, given the good openness and light transmission of liquid plasticine (LP), which is a liquid entity formed by SiO2 nanoparticles and encapsu...
Myocardial fibrosis progression and imbalanced redox state are closely associated with increased extracellular matrix (ECM) stiffness. Candesartan (CAN), an angiotensin II (Ang II) receptor inhibitor, has shown promising anti-fibrosis and antioxidant efficacy in previous cardiovascular disease studies. However, the effect of ECM stiffness on CAN ef...
Cardiac tissue is sensitive to and can be easily damaged by exogenous electric stimulation. However, due to the thermal-electric coeffect and the limitation of in situ and quantitative information on the cardiac tissue function under electric stimulation, the detailed effect and the underlying mechanism of exogenous electric stimulation on the card...
Ferroptosis, as a promising therapeutic strategy for cancers, has aroused great interest. Quantifying the quick dynamic changes in key parameters during the early course of ferroptosis can provide insights for understanding the underlying mechanisms of ferroptosis and help the development of therapies targeting ferroptosis. However, in situ and qua...
Central nervous system is sensitive and vulnerable to heat. Oxidative state and oxidative damage of neurons under heat stress are vital for understanding early consequences and mechanisms of heat‐related neuronal injury, which remains elusive partly due to the technical challenge of in situ and quantitative monitoring methods. Herein, a temperature...
The activation of Yes-associated protein (YAP) pathway is mutually causal with the increase of extracellular matrix (ECM) stiffness. Polydatin (PD) has been proved to have anti-fibrosis effect in diabetic kidney disease (DKD), but it is still a mystery whether PD participates in YAP-related mechano-transduction. Therefore, this study intends to sol...
Bacterial biofilm, a highly structured communities with developmental processes from cell adherence to highly dense biofilms, is of critical concern in many industries. Investigations of parameters mediating biofilm formation, interactions between bacterial species, and parameters governing biofilm stability are quite important for understanding me...
Deep skin wound needs a long wound healing process, in which external force on skin around wound can result in a sharp pain, wound re-damage and interstitial fluid flowing out, increasing the risk of deterioration and even amputation. While the conventional wound dressings cannot provide timely feedback of abnormal wound status and lose best time f...
Correction for ‘ In situ monitoring of functional activity of extracellular matrix stiffness-dependent multidrug resistance protein 1 using scanning electrochemical microscopy’ by Shuake Kuermanbayi et al. , Chem. Sci. , 2022, https://doi.org/10.1039/d2sc02708a.
Extracellular matrix (ECM) stiffness affects the drug resistance behavior of cancer cells, while multidrug resistance protein 1 (MRP1) on the cell membrane confers treatment resistance via actively transporting drugs out of cancer cells. However, the relationship between ECM stiffness and MRP1 functional activity in cancer cells remains elusive, ma...
In vitro cardiac tissue model holds great potential as a powerful platform for drug screening. Respiratory activity, contraction frequency, and extracellular H2O2 levels are the three key parameters for determining the physiological functions of cardiac tissues, which are technically challenging to be monitored in an in situ and quantitative manner...
Detection at single-cell level plays a critical role in revealing cell behavior in different organisms. Nanoelectrodes with high temporal–spatial resolution can precisely and dynamically monitor the physiological and pathological processes of various single cells. The field of using nanoelectrodes in single-cell electroanalysis is blooming in recen...
Lateral flow assays (LFAs), a popular point-of-care testing platform, have found widespread applications from laboratory to clinics. However, LFA-based testing is still subject to limited detection sensitivity, especially for classical gold nanoparticle-based LFAs. Inspired by traditional pen-based writing technologies, we developed a ball pen writ...
As the increase of the world's population, the desired mode of in vitro diagnostics has shifted from the execution in specialist laboratory to self‐tests, which can be assigned to one type of the so‐called “point‐of‐care testing (POCT)”. This diagnostic technique helps to tackle the matter of urgency for the place where the clinics are fewer and fa...
Detection of biomarkers associated with body conditions provides in‐depth healthcare information and benefits to disease management, where the key challenge is to develop a minimally invasive platform with the ability to directly detect multiple biomarkers in body fluid. Dermal tattoo biosensor holds the potential to simultaneously detect multiple...
Point-of-care testing (POCT) of proteins is crucial for on-site and rapid diagnosis of diseases in community and family medicine. Microfluidic paper-based analytical devices (µPADs) have attracted growing attention in POCT of proteins, while the practical applications of µPADs in the analysis of proteins in physiological samples is still limited as...
Cardiac fibrosis, in which cardiac fibroblasts differentiate into myofibroblasts, leads to oversecretion of the extracellular matrix, results in increased stiffness, and facilitates disequilibrium of cellular redox state, further leading to oxidative stress and various degrees of cell death. However, the relationship between the matrix stiffness an...
Stretchable conductors are susceptible to wear through repeated deformation over time. Stretchable conductors with self-healing properties can increase longevity and reduce safety hazards. However, most current self-healing conductors can only repair either the conductive layer or the insulating layer. Meantime, high mechanical robustness and self-...
Scanning ion conductance microscopy (SICM) as an emerging non-contact scanning probe microscopy technique and featuring its strong in-situ detectability for soft and viscous samples, is increasingly used in biomedical and materials related studies. In SICM measurements, employing theta pipette as SICM probe to scan sample is an effective method to...
Investigation of the abnormal behavior of pathological cells and tissues can provide important references for understanding the pathological mechanism of critical diseases and screening new drugs. Scanning electrochemical microscopy (SECM), an electrochemical principle-based scanning probe microscope, can record the currents or potentials when the...
On-site protein analysis is crucial for disease diagnosis in community and family medicine in which microfluidic paper-based analytical devices (μPADs) have attracted growing attention. However, the practical applications of μPADs in protein analysis for physiological samples with high complexity is still limited. Herein, we developed a three-dimen...
Multiplex and rapid detection of biomarkers in gingival crevicular fluid (GCF) is critical to achieve accurate and timely diagnosis of periodontitis severity and progression. Aiming at providing a point-of-care testing (POCT) platform for clinical diagnosis of periodontitis at chair side, we developed a disk-like lateral flow immunoassay strip (LFI...
Ruihua Tang Li Na Liu Min Li- [...]
Fei Li
Multilayered and stacked cellulose paper has emerged as a promising platform for construction of three-dimensional (3D) cell culture due to its low cost, good biocompatibility and high porosity. However, its poor light transmission makes it challenging to directly and clearly monitor cell behaviors (e.g., growth and proliferation) on the paper-base...
Detection of hydrogen peroxide (H2O2) released from cardiomyocytes is vital for pathological and physiological studies of cardiovascular diseases (CVD). Electrochemiluminescence (ECL) method has been widely utilized to monitor cellular H2O2 owing to its advantages of high sensitivity and specificity. In this work, we proposed a novel ECL biosensor...
The extracellular matrix (ECM) stiffness has direct effect on cancer cells homeostasis (e.g., cell volume), which is critical for regulation of their migration. However, the relationship among ECM stiffness, cell volume and cancer cell migration in three-dimensional (3D) microenvironment remains elusive. In this work, we prepared the collagen-algin...
Single cell analysis is important for understanding fundamental biological processes and mechanisms. Scanning electrochemical microscopy (SECM) and scanning ion conductance microscopy (SICM) as two kinds of scanning probe microscopy with high temporal and spatial resolutions, in situ and noninvasive characterization capabilities, emerge as strong t...
Developing miniaturized and rapid protein analytical platforms is urgently needed for on-site protein analysis, which is important for disease diagnosis and monitoring. Liquid marbles (LMs), a kind of particle-coated droplets, as ideal micro-reactors have been used in various fields. While their application as analytical platforms is limited due to...
Protein analysis has found widespread applications in biomedical fields ( e.g. , disease diagnosis and precision medicine), especially recently in point-of-care testing (POCT). Conventional protein analysis is large-equipment-dependent, which limits its applications in POCT. Herein, we proposed a strategy using a shaped liquid entity, termed liquid...
Mechanical microenvironment plays a key role in regulation of phenotype and function of cardiac cells, which are strongly associated with intracellular redox mechanism of cardiomyocytes. However, the relationship between the redox state of cardiomyocytes and their mechanical microenvironment remains elusive. In this work, we used polyacrylamide (PA...
He Rongyan Yan Niu Zedong Li- [...]
Fei Li
Skin interstitial fluid (ISF) holds great potential as a highly desirable sample resource for point‐of‐care testing (POCT), where the key is to effectively collect target biomarkers from ISF. Hydrogel microneedle patch has great promise for ISF extraction. However, it is challenging to recover target biomarkers from the extracted skin ISF in hydrog...
DNA methylation markers are quantified with digital PCR and applied in liquid biopsy for cancer early diagnosis.
Early diagnosis of cancer can increase the survival rate of patients. Minimally invasive liquid biopsy can avoid the problems of invasion and heterogeneity of traditional tumor biopsy methods, also is becoming a new approach for cancer diagnosis. In addition, DNA methylation as a marker to predict the occurrence and development of cancer has arouse...
Lateral flow assays (LFAs) have been extensively used as point-of-care testing platforms because they are inexpensive, portable, simple, and rapid, which particularly improve their availability in resource-poor settings. However, the poor sensitivity of LFAs restricts their further applications. Herein, we proposed a novel and simple method to enha...
One major conundrum that impedes the development and application of the emerging soft and stretchable electronics lies in the integration of electronic components with soft substrates for rational combination of various device functionalities into a single wearable state, since the rigid, nondeformable electronics tend to detach from the deformable...
The physical confinement of cell microenvironment could enhance the invasive capability and drug resistance of cancer cells. However, due to the lack of in vitro experimental platform to mimic both stiffness and confinement of the tumor microenvironment, the underlying mechanism remains elusive. Here, we developed a hydrogel-based microchannel plat...
Paper is a widely used support for use in devices for point-of-care testing (POCT) in clinical diagnosis, food safety monitoring and environmental pollution. Paper is inexpensive, biocompatible, biodegradable and allows a sample fluid to flow by capillary force. Numerous method have been developed recently for chemical modification of papers in ord...
Immunoassay has been routinely used in hospitals and central labs. Nowadays, to further meet the requirement of widespread applications of immunoassays, it is urgently needed to produce a simplified, rapid and low-cost immunoassay to perform tests on site. To this end, paper-based point-of-care (POC) immunoassays have attracted intensive interests...
Point-of-care testing (POCT) of neurotransmitters, such as dopamine (DA) and 5-hydroxytryptamine (5-HT), can be used for early diagnosis of neurological diseases. Among various POCT platforms, electrochemical method-based platforms have attracted increasing interest owning to their high detection sensitivity and specificity as well as fast response...
Upconversion nanoparticle-based lateral flow assays (UCNP-LFAs) have attracted significant attention in point-of-care testing (POCT) applications, due to the long-term photostability and enhanced signal-to-background noise ratio. The existing UCNP-LFAs generally require peripheral equipment for exciting fluorescent signals and reading out fluoresce...
Neurotransmitters are important biological molecules related to several nervous system diseases (NSDs). Point-of-care testing (POCT) of neurotransmitters is of great importance in preclinical research and early diagnosis of NSDs. Among various POCT platforms, paper-based electrochemical biosensors have achieved great advances in detection of neurot...
Hydrogel microwell arrays (HMAs) have been wildly used for engineering cell microenvironments by providing well-controlled biophysical and biochemical cues (e.g., three-dimensional (3-D) physical boundary, biomolecule coating) for cells. Among these cues, the oxygen microenvironment has shown great effect on the cellular physiological processes. Ho...
Paper-like materials have found widespread applications in various fields, especially recently emerging applications in biomedicine as paper-based devices, where liquid wicking behavior plays a significant role. Although tremendous experimental evidence has indicated that fluid control is a key technology to improve the performance of these paper-b...
Simultaneous detection of multiple targets with different analyte-recognition reactions in the same sample in a rapid, low-cost and reliable way has immense potential with widespread applications in food safety, medical diagnostics and environmental monitoring. Herein, we developed a lateral flow aptamer assay (LFAA) integrated smartphone-based por...
Nowadays, point-of-care testing (POCT) techniques have achieved flourishing development in human health monitoring, where paper-based POCT platforms have exhibited superior properties of low cost, easy accessibility and eco-friendliness. For fabrication of paper-based POCT platforms, the pen-on-paper (PoP) strategies through directly writing desire...
The utility model provides a kind of digital pcr chip based on hydrogel: the chip includes miniature hydrogel array, polymethyl methacrylate cover board, double-sided adhesive middle layer and substrate of glass, the miniature hydrogel array contains 2300 to 46000 microgels, and the single volume that microgel is lyophilized is 0.2~5 nanoliter.It w...
Liquid biopsy of cancers using DNA methylation biomarkers has received significant interests, where quantification of multiple biomarkers is generally needed for improving sensitivity and specificity of cancer diagnosis. However, inefficiency of the traditional quantitative polymerase chain reaction (qPCR)-based MethyLight assay for detecting extre...
DNA methylation biomarkers are quantified with digital PCR and applied to breast tumors In Vitro diagnosis.
Periodontitis has become one of the most universal chronic inflammatory diseases worldwide. Subclinical symptom progression, ultimately leading to permanent damage, calls for early diagnosis and long-term monitoring. However, traditional clinical diagnostic methods are complex and expensive, and cannot meet these requirements. Recently, with more b...
Point-of-care biosensors are important tools developed to aid medical diagnosis and testing, food safety and environmental monitoring. Paper-based biosensors, especially nucleic acid-based lateral flow assays (LFA), are affordable, simple to produce and easy to use in remote settings. However, the sensitivity of such assays to infectious diseases h...
In this work, we developed a microwell dPCR based MethyLight assay and tested its capability in DNA methylation analysis for cancer diagnosis. Using synthesized DNA samples, the assay achieves 1000-10000 folds lower limit of detection and 1-16 folds lower limit of quantification than those of the qPCR based assays. As for clinical samples, dPCR bas...
Gallium-based liquid metal has captivated exceptionally keen interest in recent years since it remains liquid phase at room temperature and thus conforms to the surrounding ambience. Meanwhile, such morphing capability can be tuned via altering the surface tension of liquid metal, which further triggers enthusiasm for investigating its locomotion....
The cell microenvironment has emerged as a key determinant of cell behavior and function in development, physiology, and pathophysiology. The extracellular matrix (ECM) within the cell microenvironment serves not only as a structural foundation for cells but also as a source of three-dimensional (3D) biochemical and biophysical cues that trigger an...
Paper-based microfluidic biosensors have recently attracted increasing attentions in point-of-care testing (POCT) territories benefiting from their affordable, accessible and eco-friendly features, where technologies for fabricating such biosensors are preferred to be equipment free, easy-to-operate and capable of rapid prototyping. In this work, w...
Nucleic acid testing (NAT) has been studied extensively in paper-based diagnostics, presenting a substantially higher sensitivity and specificity than immunoassays. Paper-based NAT provides an alternative to laborious, expensive and time-consuming conventional NAT. Recent advances in paper fabrication and modification technologies have made it poss...
The ever-growing overlap between electronics and wearable technology is driving the demand for utilizing novel materials that are cost-effective, light-weight, eco-friendly, mechanically deformable and can be conformably and comfortably worn on human body as substrate to support the reliable operation of wearable electronic functionalities. Paper m...
Cancer, as the most serious threat to human health, poses millions of deaths in the world each year. In vitro cancer cell and biomarker detection is of great importance for early cancer diagnosis and therapy, which needs highly sensitive, selective and quantitative diagnosis platforms. Three-dimensional paper-based electrochemical devices (3D-PEDs)...
Fe3O4 anisotropic nanostructures which exhibit excellent catalytic performance are rarely used in Fenton-like reactions, as a consequence of the inevitable drawbacks from the traditional preparing methods. In this study, we develop a facile, nontoxic, water-based approach for directly regulating a series of anisotropic morphologies of Fe3O4 nanostr...
The emerging demand for cost-effective, easily accessible, and rapid prototyping electronics fabrication calls for novel techniques to design and manufacture electronic components and devices for wearable functional sensing, on-skin medical monitoring, and body-worn energy conversion. Inspired by daily hand-writing, innovative and ubiquitously avai...
Due to the emerging demand for methods of fabricating electronics in a cost-effective, easily accessible way that allows for rapid prototyping way, novel techniques to design and manufacture electronics are required. On page 165, F. Li, F. Xu, and co-workers review the recent innovations allowing ubiquitously available pens to be employed to direct...
Trapping of microparticles finds wide applications in numerous fields. Microfluidic chips based on a dielectrophoresis (DEP) technique hold several advantages for trapping microparticles, such as fast result processing, a small amount of sample required, high spatial resolution, and high accuracy of target selection. There is an unmet need to devel...
The integration of paper with electrochemical device has attracted growing interests for point-of-care testing, where it is of great importance to fabricate electrodes on paper in a low-cost, easy and versatile way. In this work, we report a simple strategy for directly writing electrodes on paper using a pressure-assisted ball pen to form a paper-...
Owing to the shape-dependent catalytic activity of Fe3O4 nanoparticles, controlling their morphology is of great significance. In this work, we propose a simple, nontoxic, water-based strategy for the fabrication of magnetic nanoparticle/hydrogel nanocomposites in which highly crystalline Fe3O4 nanooctahedra can be fabricated in situ within a negat...
Since smart phones have been developed, significant advances in the function of mobile phone due to the development of software, hardware and accessories have been reached. Till now, smart phones have been engaged in daily life with an increasing impact. As a new medical model, mobile phone medicine is emerging and has found wide spread application...
Jie Hu Shuqi Wang lin Wang- [...]
Feng Xu
Advanced diagnostic technologies, such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), have been widely used in well-equipped laboratories. However, they are not affordable or accessible in resource-limited settings due to the lack of basic infrastructure and/or trained operators. Paper-based diagnostic technologie...
Lateral flow assays (LFAs) as rapid analytical techniques promise to be widely used in point-of-care (POC) diagnostics because of their affordability and simplicity. However, LFAs still suffer from low sensitivity in detection of various biomarkers, e.g., nucleic acids. In this study, we developed a simple and general one-step signal amplification...
A dextran-based self-healing hydrogel is prepared by reversible Diels-Alder reaction under physiological conditions. Cytocompatible fulvene-modified dextran as main polymer chains and dichloromaleic-acid-modified poly(ethylene glycol) as cross-linkers are used. Both macro- and microscopic observation as well as the rheological recovery test confirm...