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Fabrizio Stefani

Fabrizio Stefani
Water Research Institute, National Research Council, Italy

Ph.D. in Environmental Science

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74
Publications
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2,258
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Publications

Publications (74)
Article
During the first period of the SARS-CoV-2 pandemic, the lack of specific therapeutic treatments led to the provisional use of a number of drugs, with a continuous review of health protocols when new scientific evidence emerged. The management of this emergency sanitary situation could not take care of the possible indirect adverse effects on the en...
Article
Full-text available
During the first period of the SARS-CoV-2 pandemic, the lack of specific therapeutic treatments led to the provisional use of a number of drugs, with a continuous review of health protocols when new scientific evidence emerged. The management of this emergency sanitary situation could not take care of the possible indirect adverse effects on the en...
Article
The presence of SARS-CoV-2 in raw wastewaters has been demonstrated in many countries affected by this pandemic. Nevertheless, virus presence and infectivity in treated wastewaters, but also in the receiving water bodies is still poorly investigated. In this study, raw and treated samples from three wastewater treatment plants, and three river samp...
Article
Freshwater fish communities are impacted by multiple pressures, determining loss of functional diversity and redundancy. Our aim was to disentangle the roles and relevancies of different pressures in shaping fish communities in small streams of the Po plain (North Italy). Long term trend (1998–2018) of functional diversity of 31 fish communities wa...
Preprint
Full-text available
Wastewater-based epidemiology has been proposed to monitor the diffusion and trend of SARS-CoV-2 pandemic. In the present study, raw and treated samples from three wastewater treatment plants, and two river samples characterized the Milano Metropolitan Area, Italy, were surveyed for SARS-CoV-2 RNA positivity to real time PCR and infectiveness. More...
Article
Full-text available
Sediment toxicity plays a fundamental role in the health of inland fish communities; however, the assessment of the hazard potential of contaminated sediments is not a common objective in environmental diagnostics or remediation. This study examined the potential of transcriptional endpoints investigated in zebrafish (Danio rerio) exposed to riverb...
Article
Space exploration is demanding longer lasting human missions and water resupply from Earth will become increasingly unrealistic. In a near future, the spacecraft water monitoring systems will require technological advances to promptly identify and counteract contingent events of waterborne microbial contamination, posing health risks to astronauts...
Article
Composite sediment samples were collected from 18 deposition areas located in seven lagoons of the Po Delta. Samples were analysed for contents of legacy (DDT, PCB) and priority organic pollutants (AP, PBDE), and the sediments showed, with some exceptions, low/moderate levels of contamination. It seemed evident that the Po River has a clear role in...
Article
A comprehensive analytical screening has been applied for the first time to surficial sediment samples collected from seven lagoons of the Po River Delta. This screening has been used to gain further insights into the exposure conditions potentially present in the Po lagoons. Despite a certain degree of variability was observable within the lagoons...
Preprint
Full-text available
Space exploration is demanding longer lasting human missions and water resupply from Earth will become increasingly unrealistic. In a near future, the spacecraft water monitoring systems will require technological advances to promptly identify and counteract contingent events of waterborne microbial contamination, posing health risks to astronauts...
Article
Mitochondrial dloop and nuclear tnfa II sequences were sequenced from brown trout collected in the Vesta River (Italian Alps), situated in the middle of a Pleistocene ice free refugia area east of the Lake Garda. Haplotypes belonging to the native Adriatic lineage were mixed with other from the Atlantic and Marble lineages. A probabilistic approach...
Article
A multigenerational test with Chironomus riparius was performed to assess long‐term effects on life‐traits of exposure to selected perfluorinated compounds: PFOS, PFOA and PFBS. These persistent contaminants are widespread in aquatic ecosystems at low concentrations, possibly exerting long‐term toxicity. Larvae of C. riparius of a native population...
Article
The lagoons of the Po River delta are potentially exposed to complex mixtures of contaminants, nevertheless, there is a substantial lack of information about the biological effects of these contaminants in the Po delta lagoons. These environments are highly dynamic and the interactions between chemical and environmental stressors could prevent the...
Article
Full-text available
Borrowing the approaches of population genetics, evolutionary toxicology was particularly useful in assessing the transgenerational effects of a substance at sublethal concentrations, as well as evaluating genetic variation in populations exposed to pollutants. Starting from assays in controlled conditions, in recent years this approach has also fo...
Article
In this study, we characterized the gene expression responses of the Padanian barbel (Barbus plebejus), a native benthivorous cyprinid with a very compromised presence within the fish community of the River Po. Barbel juveniles were exposed in the laboratory to two river sediments reflecting an upstream/downstream gradient of increasing contaminati...
Article
Scleractinian corals (i.e. hard corals) play a fundamental role in building and maintaining coral reefs, one of the most diverse ecosystems on Earth. Nevertheless, their phylogenies remain largely unresolved and little is known about dispersal and survival of their planktonic larval phase. The small subunit ribosomal RNA (SSU rRNA) is a commonly-us...
Article
Background: In 2015 a new device for the collection of mediastinal fluid from patients with deep sternal wound infection (DSWI) in the presence of negative-pressure wound therapy (NPWT) became available. The present study was designed to evaluate whether changing sample collection devices increased micro-organism detection in patients undergoing N...
Article
Full-text available
The large estuary that the River Po forms at its confluence into the Adriatic Sea comprises a multitude of transitional environments, including coastal lagoons. This complex system receives the nutrients transported by the River Po but also its load of chemical contaminants, which may pose a substantial (eco)toxicological risk. Despite the high eco...
Article
Full-text available
The formation of foams on lakes is a complex phenomenon whose origin is often hardly identifiable. Recently (2007, 2008, and 2010) foam episodes started to occur in Lake Maggiore, northern Italy. The present work aimed to verify the hypothesis of an endogenous-natural origin of these foams, driven by trophic or climatic changes. To this purpose, a...
Article
Full-text available
This paper reviews the scientific knowledge on the use of a lanthanum modified bentonite (LMB) to manage eutrophication in surface water. The LMB has been applied in around 200 environments worldwide and it has undergone extensive testing at laboratory, mesocosm, and whole lake scales. The available data underline a high efficiency for phosphorus b...
Article
Alpine ecosystems are especially vulnerable to climate change, and lakes and ponds act as early indicators. Here, we describe our findings for the Ortles-Cevedale mountain group (Stelvio National Park, Southern Alps, Italy), where we used remote sensing to analyze more than 100 water ponds over the last 50 years (1954–2007). We found that since the...
Article
Full-text available
A new scleractinian coral species, Pachyseris inattesa sp. n., is described from the Red Sea. Despite a superficial resemblance with some species in the agariciid genus Leptoseris with which it has been previously confused, P. inattesa sp. n. has micro-morphological characters typical of the genus Pachyseris. This genus, once part of the Agariciida...
Article
Recent molecular phylogenetic studies have shown that most traditional families of zooxanthellate shallow-water scleractinians are polyphyletic, whereas most families mainly composed of deep-sea and azooxanthellate species are monophyletic. In this context, the family Dendrophylliidae (Cnidaria, Scleractinia) has unique features. It shows a remarka...
Article
Full-text available
The Indo-Pacific coral genus Blastomussa (Cnidaria: Anthozoa: Scleractinia) includes three species, i.e., B. merleti, B. wellsi, and B. loyae. Following the re-examination of relevant type material, other museum specimens, and the study of newly sampled corals, the genus is revised and the new species B. vivida is described. The new species differs...
Article
Full-text available
Stylophora pistillata is a widely used coral "lab-rat" species with highly variable morphology and a broad biogeographic range (Red Sea to western central Pacific). Here we show, by analysing Cytochorme Oxidase I sequences, from 241 samples across this range, that this taxon in fact comprises four deeply divergent clades corresponding to the Pacifi...
Article
Full-text available
The monotypic genus Craterastrea was assigned to the family Siderastreidae owing to the similarity of its septal micromorphology to that of Coscinaraea. Subsequently, it was synonymized with Leptoseris, family Agariciidae, based on corallum macromorphology. Since then, it has remained poorly studied and has been known only from a small number of sp...
Article
A new zooxanthellate reef-dwelling scleractinian coral species, Porites fontanesii sp. nov. (Scleractinia, Poritidae), is described. The examined material was collected from the Southern Red Sea, the Gulf of Tadjoura, and the Gulf of Aden. Porites fontanesii sp. nov. was most frequently observed along the Yemen south Red Sea and the northwestern Gu...
Article
Full-text available
Genetic studies of Lessepsian species often demonstrate the absence of a genetic bottleneck in a wide plethora of taxa, from plants to fish, but information regarding the genetic responses of their parasites in the newly colonized ecosystems is still lacking. This study compared genetic diversity of Red Sea (Eilat, Nabq), Suez canal (Ismailia) and...
Article
Full-text available
The scleractinian species Psammocora explanulata and Coscinaraea wellsi were originally classified in the family Siderastreidae, but in a recent morpho-molecular study it appeared that they are more closely related to each other and to the Fungiidae than to any siderastreid taxon. A subsequent morpho-molecular study of the Fungiidae provided new in...
Article
Full-text available
Searching for nestedness has become a popular exercise in community ecology. Significance of a nestedness index is usually evaluated using z values, and finding that a matrix is nested is typically a common result. However, nestedness is not likely to be spread uniformly within a matrix of species presence/absence per site. Selected parts of the ma...
Article
A combined morphological and genetic study of the coral genus Stylophora investigated species boundaries in the Gulf of Aden, Yemen. Two mitochondrial regions, including the hypervariable IGS9 spacer and the control region, and a fragment of rDNA were used for phylogenetic analysis. Results were compared by multivariate analysis on the basis of bra...
Article
Full-text available
Until coral molecular phylogenies were published, the genus Plesiastrea was traditionally part of the family Faviidae and considered by several authors to be closely related to the genus Montastraea. However, genetic data has shown that Plesiastrea versipora, the genus type species, is evolutionarily distinct within the Robust clade of the Scleract...
Article
A 22-month study (2008-2009) was carried out on 273 patients (average age 40 months), admitted with gastroenteritis to the Pediatric Unit of L. Sacco University Hospital in Milan, Italy. Fecal samples were investigated for rotavirus (HRV), norovirus (NoV), adenovirus (AdV), sapovirus (SaV), enterovirus, astrovirus and bocavirus (HBoV). A 38.3% inci...
Article
Full-text available
A new statistical technique able to localize nestedness within a matrix is used to reapproach the 'centre of origin' concept. Finding that nestedness is concentrated in a particular sector of a matrix indicates that widespread species tend to be present in the species-richest area. A 'centre of origin' hypothesis, which assumes that species have a...
Article
Full-text available
The genetic variability among Italian populations of the white-clawed crayfish (Austropotamobius pallipes) was examined to determine their phylogeography and to assess their conservation status as a management unit. A fragment of the mitochondrial DNA COI gene of 107 specimens from ten populations was sequenced, and the phylogenetic relationships w...
Article
Full-text available
An updated checklist of the monogenoids infecting Italian marine fish is provided, including seven new records (Diplectanum simile on Sciaena umbra; Diplectanum sp. on Scorpaena porcus; Lamellodiscus elegans, L. ergensi, L. fraternus on Diplodus vulgaris; L. virgula on Pagellus erythrinus; Pseudanthocotyloides heterocotyle on Engraulis encrasicolus...
Article
The morphometric and molecular boundaries between twelve Psammocora (Cnidaria, Scleractinia) nominal species were addressed. The type specimens of Psammocora haimianaMilne Edwards & Haime, 1851, P. togianensisUmbgrove, 1940, P. foliumUmbgrove, 1939, P. digitataMilne Edwards & Haime, 1851, Maeandroseris australiaeRousseau, 1854, P. samoensisHoffmeis...
Article
Full-text available
An eighteen-month study (2008-2009) was carried out on patients, aged on average 30 months, admitted with gastroenteritis to the Paediatric Unit of the L. Sacco Hospital in Milan, Italy. Faecal samples of 154 patients were investigated for Rotavirus, Norovirus,Adenovirus, Enterovirus and Bocavirus. A total of 25 norovirus positive samples were sele...
Article
Full-text available
A new record of the freshwater jellyfish Craspedacusta sowerbii in Northern Italy is reported. The medusae bloom occurred in a small oligo-mesotrophic artificial lake during July, 2009. At the time of sampling, the medusae were all immature, reached low density and showed feeding preference towards the Daphnia complex of species, the most abundant...
Article
Full-text available
A host-parasite relationship was observed, for the first time, between a piscicolid leech and a species of amphibious goby (Scartelaos tenuis) from an intertidal mud flat in southern Iran. Morphological and molecular investigations assign the leech to Zeylanicobdella arugamensis. Of the 3 endemic and sympatric mudskipper species living in the Persi...
Article
Full-text available
In the present work we examined the efficacy of three different chemical solutions (EtOH 70%, DMSO-NaCl solution, and Longmire buffer) in field preservation of fish gills to be subsequently screened for monogenean specimens destined to morphological and molecular analyses. Degree of difficulty in collecting monogeneans from gills, morphological sta...
Article
Full-text available
A comparative morphological and molecular characterization of species boundaries between four species of the Scleractinian genus Psammocora, namely P. digitata, P. haimeana, P. profundacella and P. contigua was conducted. The definition of species boundaries in this genus has been the subject of few studies, in spite of the presence of ambiguous ta...
Article
Full-text available
Integrative taxonomy is currently recognised by many as the solution to the “taxonomic crisis”. However, the use of a multi-disciplinary approach to the study of species boundaries in Scleractinia has been, so far, limited. Molecular techniques have provided new opportunities to explore taxonomic boundaries and the phylogeny of taxa traditionally i...
Article
Cladocora caespitosa (L.,1767) is the principal endemic bioconstructor coral of the Mediterranean Sea. Despite recent episodes of mortality have been documented all over the Mediterranean and a significant knowledge about the ecology, paleoecology and conservation status of this species exists, no description of its phylogeny and phylogeography is...
Article
Full-text available
The Indo-Pacific coral genus Psammocora Dana (1846) has never been formally revised, and its phylogeny has only been partially explored. Several synonymies have been proposed for the 11 nominal species which have highly plastic branching growth forms. In the present study, the definition of genetic and morphologic boundaries among three currently r...
Article
The phylogeographical pattern of vairone (Telestes muticellus) populations was assessed to test the biogeographical distinction of three peri-Mediterranean ichthyogeographical districts: Padano-Venetian (PV), Tuscano-Latium (TL) and southern France (SF), evaluating the role of Alpine and Apennine barriers in shaping distinct evolutionary lineages....
Article
Full-text available
Sixteen species of both native and alien fish were collected and examined for monogenoids from September 2001 to March 2004 in bodies of water located within fishing areas of the Po River (northern Italy). A checklist of monogenoids was compiled and integrated with literature reports of Italian monogenoids, updating the number of known Italian fres...
Article
Full-text available
A parasitological survey of the monogenoids of 14 species of common fishes associated with the local coral reefs of the Ras Mohammed National Park, National Parks of Egypt South Sinai Sector, Egypt, was carried out from May 2003 to May 2005. The monogenoids collected during the survey included 17 species: 8 previously described species, 7 new speci...
Article
A new method of three-dimensional (3-D) analysis of sclerotised structures of monogenoids was performed by processing z-series images using 3D-Doctor. Z-series were obtained from Gomori's trichrome-stained specimens of marine and freshwater monogenoids under laser scanning confocal fluorescence microscopy. Measurements obtained from 3-D images were...
Article
The phylogenetic relationships of the scleractinian genus Psammocora with the other genera traditionally included in the family Siderastreidae and some Fungiidae are assessed based on combined skeletal and molecular data. P. explanulata differs from the other examined congeneric species (P. contigua, P. digitata, P. nierstraszi, P. profundacella, P...
Article
Although the use of the water resources in Uzbekistan is strongly limited by their quality, it has to be noted that there is a lack of information and data within the international scientific literature with regard to the water chemical characteristics of the Amu Darya River, one of the main water resources in Central Asia. To add to such knowledge...
Article
Full-text available
A nondestructive protocol for preparing specimens of Monogenoidea for both alpha-taxonomic studies and reconstruction of 3-dimensional structure is presented. Gomori's trichrome, a stain commonly used to prepare whole-mount specimens of monogenoids for taxonomic purposes. is used to provide fluorescence of genital spines, the copulatory organ, acce...
Article
Amu Darya river, one of the main water resources of Uzbekistan, shows a relevant longitudinal enrichment of soluble contents which strongly limits the human uses of its waters. Because of the low natural run-off processes, salts and pollutants are mainly driven to the river by the return waters used for washing and irrigating the surrounding lands....
Article
When a species is introduced in a new territory its parasites may also follow, thus forming a sort of biotic unit. We propose the term symbiota to indicate this host–parasite complex. This concept is at the basis of the present research in which 11 species of alien and native freshwater fish were collected by electrofishing in the Po river area (No...
Article
Full-text available
The authors have examined the morphology of Diplectanum cazauxi and Diplectanum bauchotae through the observation of 23 specimens (10 of D. cazauxi and 13 of D. bauchotae) found during May 2003 on the gills of 2 specimens of Sphyraena flavicauda Rüppell, 1838 collected at Ras Mohammed National Park, Egypt. A discriminant analysis was performed on t...
Article
Summary1. The strong contraction of the European populations of white-clawed crayfish has stimulated research of its population genetics. As part of a conservation project to introduce and improve Italian populations of Austropotamobius italicus, the genetic diversity of 16 populations from 10 tributaries of the Po River drainage was evaluated by s...
Article
Austropotamobius pallipes is a threatened species and a management plan must be put into action in order to avoid its extinction. As part of the recent conservation effort in Northern Italy, a phylogeographic and taxonomic study was deemed necessary. The sequencing of a 464 bp length of 16SmtDNA gene was used. The evolutionary topology and the pars...
Article
A phylogeographic analysis of 12 populations of Telestes muticellus was performed on the basis of mitochondrial DNA sequences of the cytochrome b gene. The main aims were to determine phylogenetic relationships from congener species and to highlight the patterns of genetic differentiation within the Italian peninsula. The results obtained showed a...