Fabrizia Mastroianni

Fabrizia Mastroianni
Università degli Studi di Milano-Bicocca | UNIMIB · Department of Biotechnology and Biosciences

About

22
Publications
803
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167
Citations
Introduction
Skills and Expertise
Additional affiliations
November 2002 - November 2005
Università della Calabria
Position
  • PhD Student

Publications

Publications (22)
Article
Full-text available
Rewiring glucose metabolism toward aerobic glycolysis provides cancer cells with a rapid generation of pyruvate, ATP, and NADH, while pyruvate oxidation to lactate guarantees refueling of oxidized NAD+ to sustain glycolysis. CtPB2, an NADH-dependent transcriptional co-regulator, has been proposed to work as an NADH sensor, linking metabolism to epi...
Article
Full-text available
Cancer cells share several metabolic traits, including aerobic production of lactate from glucose (Warburg effect), extensive glutamine utilization and impaired mitochondrial electron flow. It is still unclear how these metabolic rearrangements, which may involve different molecular events in different cells, contribute to a selective advantage for...
Data
Analytical computations supporting simulation results in Fig 6. For different possible flux routes (table rows), the P/O ratio (column 8) of as well as the moles of acetyl-CoA deriving form one mole of substrate (column 10) following the considered route is computed. In some cases, the row indicates the difference between the computations of two po...
Data
ENGRO1 SBML model. Excel file of the model compliant with: RAVEN Toolbox and FAME. (XML)
Data
ENGRO1 SVG map. Scalable Vector Graphics image of the model, compliant with the software FAME. (SVG)
Data
ENGRO1 biomass optimization for different boundary conditions (supplement to Fig 4). All data derive from Flux Variability Analysis in experiments that maximize biomass production. All reported fluxes do not show variability in optimal solutions. All glucose available is always fully consumed in these simulations. A) Growth rate (z-axis) and oxygen...
Data
Interplay among the sub-networks showing alternative flux patterns in Fig 5. The first sub-network (shaded polygon, with the exception of pyruvate carboxylase) includes PEP carboxykinase; pyruvate kinase, NADP and NAD dependent malic enzyme and malate dehydrogenase. Because the TCA cycle is working in a non-cyclic mode, this set of reactions allows...
Data
Experimental raw data. Raw data (enzyme assays, growth curves, metabolomics) used to build graphs in Fig 3. (XLS)
Data
Supplemental text. Biochemical interpretation of experimental results. (PDF)
Data
ENGRO1 excel model. Excel file of the model compliant with the RAVEN Toolbox. (XLS)
Data
Similarity of results obtained when oxygen consumption is modulated by altering either oxygen availability (as in Fig 4) or Complex I capacity (flux upper bound). Note that because of the reaction stoichiometry, Complex I flux is proportionally higher than the corresponding oxygen consumption rate. A-B) Growth rate scaled on glucose availability as...
Data
On why glutamine is the preferred anaplerotic precursor (supplement to Fig 6). A) Maximum and minimum value for lactate secretion across optimal growth solutions according to FVA, as a function of the parameter pROS that emulates the level of production of reactive oxygen species in the respiratory chain (see S1 Supplemental Methods). Glucose avail...
Data
Determination of sample size. A) Standard deviation of the model fluxes (represented with different colors) as a function of the sample size. B) Zoom in on fluxes of panel A with low standard deviations. The fluxes with high standard deviations correspond to those with high variability when running FVA. C) Relative standard error of the mean of eac...
Data
ENGRO1 flux distribution that maximizes growth at critical O2GR (O2: 38 mM h-1; G: 10 mM h-1; Q: 40 mM h-1). The optimal flux value obtained with FBA, the minimum and maximum value as well as the range size, obtained with FVA, are reported for each reaction. (XLSX)
Data
ENGRO1 flux distribution that maximizes ATP production at critical O2GR (O2: 38 mM h-1; G: 10 mM h-1; Q: 40 mM h-1). The optimal flux value obtained with FBA, the minimum and maximum value as well as the range size, obtained with FVA, are reported for each reaction. (XLSX)
Data
Supplemental methods. Details on ENGRO1 model reconstruction. (PDF)
Article
Full-text available
Oncogenic K-ras is capable to control tumor growth and progression by rewiring cancer metabolism. In vitro NIH-Ras cells convert glucose to lactate and use glutamine to sustain anabolic processes, but their in vivo environmental adaptation and multiple metabolic pathways activation ability is poorly understood. Here, we show that NIH-Ras cancer cel...
Article
In this study we have reported that bergapten (B) and bergapten plus UV (PUVA) are able to significantly affect MCF-7, ZR-75 and SKBR-3 breast cancer cell proliferations. B induced a lowering of PI3K/AKT survival signal in MCF-7 cells even in presence of IGF-I stimulation. Furthermore, B and in a higher extent, PUVA up-regulated the p53 mRNA and th...
Article
In the present study, we evidence how in breast cancer cells low doses of Taxol for 18 h determined the upregulation of p53 and p21 waf expression concomitantly with a decrease of the anti-apoptotic Bcl-2. P53 and its gene product, the mdm2 protein, in treated cells exhibits a prevalent nuclear compartmentalization, thus potentiating p53 transactiv...
Article
Full-text available
The expression of estrogen receptor alpha (ERalpha) is generally associated with a less invasive and aggressive phenotype in breast carcinoma. In an attempt to understand the role of ERalpha in regulating breast cancer cells invasiveness, we have demonstrated that cell adhesion on fibronectin (Fn) and type IV Collagen (Col) induces ERalpha-mediated...

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