Fabiola Branco Filippin Monteiro

Fabiola Branco Filippin Monteiro
Federal University of Santa Catarina | UFSC · Departamento de Análises Clínicas

PhD

About

55
Publications
14,515
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851
Citations
Introduction
Research activities are focused on identifying mechanisms responsible for obesity associated metabolic disease with a view of developing preventive and treatment strategies for these disorders including Type-2 diabetes mellitus and cardiovascular diseases. Obesity leads to a chronic low-grade inflammation in the adipose tissue, which is in part responsible for the development of whole-body insulin resistance. The major focus of our research program is to uncover these mechanisms.
Additional affiliations
March 2013 - present
Federal University of Santa Catarina
Position
  • Professor (Assistant)
Description
  • Clinical Immunology
January 2011 - present
Federal University of Santa Catarina
March 2007 - March 2012
University of São Paulo
Position
  • PhD Student
Education
January 2007 - January 2011
Independent Researcher
Independent Researcher
Field of study
  • Pharmacy

Publications

Publications (55)
Article
Full-text available
Exclusive breast milk is the diet recommended by the World Health Organization (WHO) until 6 months of age. However, breastfeeding has the potential of transferring certain toxic chemicals from the mother to the infant. Bisphenol A (BPA) is a synthetic chemical used as a monomer in polycarbonate plastics and epoxy resins. Information on BPA concent...
Article
Full-text available
Abstract Here we examined whether serum from obese patients could create a growth-enhancing microenvironment that alters gene expression in BRAF- and NRAS-mutated melanoma cell lines. SK-Mel-28 (BRAF-mutated) and Sk-Mel-147 (NRAS-mutated) cells were treated with pooled serum from 10 severely obese patients (BMI > 40 kg/m2), pooled serum from 6 heal...
Article
Acanthamoeba spp. are the causative agents of a sight-threatening infection of the cornea known as Acanthamoeba keratitis (AK). Amphotericin B - deoxycholate (AB) is used in the treatment of infectious keratitis, however, its topical administration has side effects as blepharitis, iritis, and painful instillation. In this context, the preheating of...
Article
A b s t r a c t We studied the effect and the mechanisms of action of 2α,3β,23-trihydroxyolean-12-ene (THO), from Croton heterodoxus Baill. (Euphorbiaceae), in glucose uptake in hyperglycemic rats. The effect of in vivo pretreatment with THO in hyperglycemic rats was analyzed. The in vitro effects of THO were observed in adipocytes and in adipose t...
Article
Objectives Obesity is a global health problem and is associated with a chronic low-grade inflammatory state. Surgical obesity treatment is being increasingly common due to its efficacy. From this, we evaluate the metabolic state improvement and inflammation remission in patients with obesity undergoing bariatric surgery. Methods: The clinical data...
Article
Full-text available
The Melanocortin-4 Receptor (MC4R) plays a pivotal role in energy homeostasis. We used human MC4R mutations associated with an increased or decreased risk of obesity to dissect mechanisms that regulate MC4R function. Most obesity-associated mutations impair trafficking to the plasma membrane (PM), whereas obesity-protecting mutations either acceler...
Article
Full-text available
The development of new treatments for malignant melanoma, which has the worst prognosis among skin neoplasms, remains a challenge. The tumor microenvironment aids tumor cells to grow and resist to chemotherapeutic treatment. One way to mimic and study the tumor microenvironment is by using three-dimensional (3D) co-culture models (spheroids). In th...
Article
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Leishmaniasis occurs in the five continents and represents a serious public health challenge, but is still a neglected disease, and the current pharmacological weaponry is far from satisfactory. Triglyceride-rich nanoparticles mimicking chylomicrons (TGNP) behave metabolically like native chylomicrons when injected into the bloodstream. Previously...
Article
Humans are potentially exposed to multiple nanoparticles kinds through nanotechnology-based consumer products. There is insufficient data on the in vivo toxicity of nanotechnology products, as well as no data on the possible toxicity, including genotoxicity and reproductive toxicity of co-exposure to different kind of nanoparticles. In this work, s...
Article
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A 31-year-old female daily user of contact lenses sought medical attention, reporting blurred vision and irritation of the left eye. Slit-lamp examination revealed hyperemia and an irregular corneal epithelium surface, and empirical treatment was started. A corneal scrape was obtained and examined for the presence of fungi, bacteria, and Acanthamoe...
Article
Full-text available
Although inflammation is a biological phenomenon that exists to protect the host against infections and/or related problems, its unceasing activation results in the aggravation of several medical conditions. Imidazoles, whether natural or synthetic, are molecules related to a broad spectrum of biological effects, including anti-inflammatory propert...
Article
Introduction The resin of Cola nitida is used in western Cameroon as incense for spiritual protection and during ritual ceremonies. This plant secretion has never been investigated although previous chemical and biological studies on other resins have drawn many attentions. Objective The resin fractions which revealed inhibitory effect on nitric o...
Article
Ethnopharmacological relevance: A prolonged estrogen deficiency alters lipid metabolism and increases risks of cardiovascular diseases. Phytoestrogens, naturally occurring compounds with estrogenic properties are reported to have cardiovascular protective effects. Millettia macrophylla used in the Cameroonian traditional system to treat physiologi...
Article
The aim of the present study was to investigate the mechanism of action of a sulfonamide derivative on glucose uptake in adipose tissue, as well as to characterize the effects of this compound on intestinal disaccharidases and advanced glycation end-products (AGEs) formation. Camphoryl-benzene sulfonamide (CS) was able to stimulate glucose uptake i...
Article
Full-text available
Natural antioxidants present in fruits have attracted considerable interest due to their presumed safety and potential nutritional value. Even though antioxidant activities of many fruits have been reported, the effects of phytochemicals of goji berry (GB) in patients with metabolic syndrome have not been investigated. In this study, we examined an...
Article
Objectives: Since complement system has been recently associated with metabolic and cardiovascular diseases, and closely related to insulin resistance, we investigated the association of plasma complement factor 3 (C3) and factor 4 (C4) with insulin sensibility and weight loss after bariatric surgery. Methods: Serum levels of C3, C4, total chole...
Article
A series of 28 aryl- and alkyl-substituted isothiouronium salts were readily synthesized in high yields through the reaction of allylic bromides with thiourea, N-monosubstituted thioureas or thiosemicarbazide. The S-allylic isothiouronium salts substituted with aliphatic groups were found to be the most effective against leukemia cells. These compo...
Article
In light of the evidence that in contrast to most healthy tissues, several neoplasms overexpress fatty acid synthase (FASN) upon their dependence on increased lipogenesis; targeting of this protein is being considered as a valuable strategy in anticancer drug development. This can be particularly relevant for aggressive tumors such as melanoma in w...
Article
In this work in vivo experiments were conducted in order to characterize the biocompatibility of polyurethane nanoparticles (PU-NPs) after intraperitoneal (i.p.) and oral administration. Additionally, ex vivo assays were performed to assess human blood compatibility as well as in vitro assays to assess protein binding. Our results indicated that ad...
Article
Maleimides consist of an important class of compounds easily synthesized with multiple functional group modification that provides expressive pharmacological properties including, antitumoral activity, mediated mainly by oxidative stress. For this reason, the present study was designed to evaluate the cytotoxicity and the role of reactive oxygen sp...
Article
À medida que há uma melhoria na tecnologia aplicada à saúde humana, a expectativa de vida vem aumentando, mas nem todas as partes do corpo podem manter suas funções com o processo de envelhecimento. A engenharia tecidual foi desenvolvida para substituir, reparar ou reconstruir tecidos ou órgãos perdidos ou danificados por acidentes ou doenças grave...
Article
Melanoma is a very aggressive type of skin cancer. Mutation in BRAF and NRAS are often found in patients with this disease. Therefore, in recent years the search for new molecules that inhibit these proteins has been intensified. After many years with no new treatments for melanoma, the U.S. Food and Drug Administration (FDA) recently approved vemu...
Article
Full-text available
Two new synthetic melanin-like oligomers inspired by the synthesis of pheomelanin (cys-DOPA) were prepared using L-DOPA as a precursor and the amino acids serine (ser-DOPA) and threonine (thr-DOPA) for modifications. The products obtained exhibited appreciable antioxidant activity. The compounds were characterized by liquid chromatography-mass spec...
Article
Full-text available
Once there is an increase on technology applied to human health, life expectancy has increased, but not all body parts can maintain their functions with aging process. Tissue engineering has developed for replacing, repairing or reconstructing lost or damaged tissues or organs by accidents or serious diseases through using and developing of new mat...
Article
Objectives To test the toxicity and antitumoral activity of the compound N-oleyl-daunorubicin (oDNR) with a cholesterol-rich nanoemulsion (LDE) formulation.MethodsLDE-oDNR was prepared by high-pressure homogenisation of lipid mixtures. B16F10 melanoma cells and NIH/3T3 fibroblasts were used for cytotoxicity tests. The maximum tolerated dose (MTD) o...
Article
Full-text available
Nine types of solid lipid nanoparticle (SLN) formulations were produced using tripalmitin (TPM), glyceryl monostearate (GM) or stearic acid (SA), stabilized with lecithin S75 and polysorbate 80. Formulations were prepared presenting PI values within 0.25 to 0.30, and the physicochemical properties, stability upon storage and biocompatibility were e...
Article
Acute lymphoblastic leukemia is a malignant disorder caused by proliferation of lymphoid progenitor cells, and is the most common cancer in children. Cytotoxic nucleoside analogues are important chemotherapeutic agents, which are used in many cancers, including leukemias. In this study, we investigated the effects of the synthetic nucleoside analog...
Article
Full-text available
The serum amyloid A (SAA) protein is known to function in the acute phase response and immunoregulation. Recently, SAA has been shown to be involved in cell proliferation, differentiation and migratory behavior in different cell types. Here, we evaluated whether exogenous SAA could influence trophoblast invasion and differentiation using both the t...
Article
Acellular biological tissues, including bovine peri- cardium (BP), have been proposed as biomaterial for tissue engineering. BP is usually modified chemically to improve mechanical and biological properties using glutaraldehyde, the standard reagent for preservation of fresh bioprosthetic materials. Glutaraldehyde-fixed BP (Glut-BP), the most widel...
Article
Full-text available
The hydrophobicity of some photosensitizers can induce aggregation in biological systems, which consequently reduces photodynamic activity. The conjugation of photosensitizers with nanocarrier systems can potentially be used to overcome this problem. The objective of this study was to prepare and characterise hypericin-loaded solid lipid nanopartic...
Article
In this work, we present a study of peroxidase adsorption on PEGylated polyurethane (PU-PEG) nanoparticles using a purified horseradish peroxidase (HRP). The influence of the immobilization on the catalytic activity of the enzyme was also investigated and the system was applied as a modified carbon paste electrode to dopamine determination in pharm...
Article
This study investigated the apoptotic effect of electronic waste on fibroblast cell line. Cells were treated with different concentrations of the leachate for 24 h. Cell viability was detected by MTT (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test, nuclear morphology of cells was explored by acridine orange (AO)/ethidium bromid...
Article
Hypoxia has been implicated as a possible cause of adipose tissue inflammation. Furthermore, the acute phase protein serum amyloid A (SAA) has been associated with the modulation of the adipogenic process, and it is well-known that obese individuals have increased levels of SAA. The effect of hypoxia in the expression and production of SAA was exam...
Chapter
Full-text available
Previous studies have demonstrated a variety of biological activity of gallic acid (GA) including antitumoral, antioxidant and antiinflammatory properties. Among these numerous data, in vitro assays showed that the GA and its derivatives are active against several types of tumour cells. Particularly, the studies have shown that the antitumor activi...
Article
Solid lipid nanoparticles (SLNs) are an alternative drug delivery system compared to emulsions, liposomes and polymeric nanoparticles. Due to their unique sizes and properties, SLNs offer possibility to develop new therapeutic approaches. The ability to incorporate drugs into nanocarriers offers a new prototype in drug delivery that could be used f...
Article
Abstract Solid lipid nanoparticles (SLNs) are an alternative drug delivery system compared to emulsions, liposomes, and polymeric nanoparticles. Due to their unique sizes and properties, SLN offer possibility to develop new therapeutic approaches. The ability to incorporate drugs into nanocarriers offers a new prototype in drug delivery that could...
Article
A hybrid material with excellent mechanical and biological properties is produced by electrospinning a co-solution of PET and collagen. The fibers are mapped using SEM, confocal Raman microscopy and collagenase digestion assays. Fibers of different compositions and morphologies are intermingled within the same membrane, resulting in a heterogeneous...
Article
This study was undertaken to address the current deficient knowledge of cellular response to solid lipid nanoparticles (SLNs) exposure. We investigated the cytotoxicity of several SLNs formulations in two fibroblast cell lineages, Vero and MDCK. Several methods were used to explore the mechanisms involved in this cytotoxic process, including cell v...
Article
Full-text available
Serum amyloid A (SAA) is an acute-phase protein that has been recently correlated with obesity and insulin resistance. Therefore, we first examined whether human recombinant SAA (rSAA) could affect the proliferation, differentiation and metabolism of 3T3-L1 preadipocytes. Preadipocytes were treated with rSAA and analyzed for changes in viability an...
Conference Paper
Full-text available
In obesity, adipocyte hypertrophy and hyperplasia might generate hypoxic areas within the tissue. In a close relationship with hypoxia, the adipose tissue produces inflammatory molecules involved in obesity-related complications, such as cardiovascular disease and metabolic disorders. Besides the hypoxia-inducible factor 1a (HIF- 1a), several prote...
Conference Paper
Full-text available
Introduction: Obesity is closely associated with a state of chronic, low-grade inflammation characterized by abnormal cytokine production and activation of inflammatory signaling pathways in adipose tissue. Also, a permanent increase in the serum amyloid A (SAA) levels is observed. As proliferation is a prerequisite for adipogenesis, we evaluate th...
Article
Block copolymers containing isosorbide succinate and l-lactic acid repeating units with different mass compositions were synthesized in two steps: bulk ring-opening copolymerization from l-lactide and poli(isosorbide succinate) (PIS) preoligomer, in the presence of tin(II) 2-ethylhexanoate as catalyst, followed by chain extension in solution by usi...
Article
Amphotericin B (AB) is the standard drug for invasive fungal infection therapy. It has a broad spectrum of activity and it is the best antifungal available against most yeasts and molds. Its therapeutic use, however, is limited by significant side effects, leading to a low therapeutic index when it is used as the traditional formulation (Fungizone®...
Article
Full-text available
A anfotericina B, fármaco descoberto em 1953, ainda permanece como substância fungicida de escolha no tratamento da maioria das micoses sistêmicas que acometem pacientes imunocomprometidos. Mesmo com a sua toxicidade e a introdução de antifúngicos azólicos sistêmicos na década de 1980, a potência, o espectro de ação e os quase 50 anos de experiênci...
Article
Full-text available
Amphotericin B, discovered in 1953, has been the antifungal drug of choice for the most fungal infections in immonosupressed patients. Despite its toxicity and the introduction of new azolic antifungal agents in the 1980's, the therapeutic potencial, the spectrum of action, and almost 50 years of clinical experience, determine its efficacy for the...
Article
The present study was designed to determine the normal range of serum beta 2-microglobulin (Sb2M) levels among healthy volunteers in Brazil. Levels of Sb2M are elevated in human immunodeficiency virus (HIV)-infected patients and have been shown to be the best predictor of HIV infection status and of some malignant disorders, especially multiple mye...
Article
Full-text available
1 -Mestranda do Programa de Pós-graduação em Farmácia (PGFAR) 2 -Alunos do Departamento de Análises Clínicas (ACL), Centro de Ciências da Saúde (CCS) 3 -Bioquímica do Laboratório de Análises Clínicas do Hospital Universitário 4 -Professora do PGFAR e do ACL-CCS, Universidade Federal de Santa Catarina Artigo Serum alanine aminotransferase (ALT) has...

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