Fabio Boylan

Trinity College Dublin | TCD · School of Pharmacy and Pharmaceutical Sciences

Motivated by the ethnopharmacological use of Pulicaria dysenterica, in the present study, the antimicrobial potential of the extracted essential oil was investigated against a panel of eighteen microorganism strains. Additionally, anti-acetylcholinesterase and antispasmodic (isolated rat dis-tal colon) activities, general acute toxicity (Artemia sa...

The ethanol extract (EE) prepared from the leaves of Tibouchina granulosa, and its fraction in ethyl acetate (fEA) were evaluated concerning their capacity to reduce inflammation in different experimental models. fEA was also studied concerning its chemical constituents. EE and fEA were assayed for their anti-inflammatory potential, using formalin-...

The ethanol extract (EE) prepared from the leaves of Tibouchina granulosa, and its fraction in ethyl acetate (fEA) were evaluated concerning their capacity to reduce inflammation in different experimental models. fEA was also studied concerning its chemical constituents. EE and fEA were assayed for their anti-inflammatory potential, using formalin-...

Helicobacter pylori is the most common cause of gastritis and peptic ulcers, and the number of resistant strains to multiple conventional antimicrobial agents has been increasing in different parts of the world. Several studies have shown that some essential oils (EO) have bioactive compounds, which can be attributed to antimicrobial activity. Ther...

Malignant ulcerating wounds or neoplastic lesions are a considerable burden for patients suffering from advanced cancer. These wounds have no effective treatment and are very difficult to manage. The present review summarizes evidence in support of a hypothesis put forward in anthroposophic medicine, which suggests a beneficial role of resin from t...

Elsholtzia ciliata (Thunb.) Hyl, family Lamiaceae, is an important and popular anti-bacterial and anti-inflammatory Traditional Chinese Medicine (TCM). However, there are limited scientific studies on its anatomy and pharmacological activities. Moreover, the information of chemical constituents in relation to its non-volatile constituents are still...

Choisya ternata Kunth variety Sundance (CTS) is a plant used in traditional medicine in North America, especially in Mexico. The present study evaluated the antinociceptive activity of the crude ethanolic extract of CTS leaves and tested its isolated compounds puberulin (Pu) and choisyine (Ch). An antinociceptive effect was observed after treatment...

A comprehensive data collection of mass spectral and gas chromatographic data of a series of esters of diastereomeric menthols (menthol, isomenthol, neomenthol, and neoisomenthol), in total 150 chemical entities, obtained by GC–MS on commonly used capillary columns of different polarity (non-polar HP-5MS and polar HP-Innowax), was created. Also, MS...

Background: Current recommendations for the self-management of SARS-Cov-2 disease (COVID-19) include self-isolation, rest, hydration, and the use of NSAID in case of high fever only. It is expected that many patients will add other symptomatic/adjuvant treatments, such as herbal medicines. Aims: To provide a benefits/risks assessment of selected he...

Method: The plant selection is primarily based on species 32 listed by the WHO and EMA, but some other herbal remedies are considered due to their widespread use in respiratory conditions. Preclinical and clinical data on their efficacy and safety were collected from authoritative sources. Target population: adults with early flu symptoms without u...

Coronaviruses belong to the subfamily Coronavirinae , which are large viruses with a single Ribonucleic acid tape and a nucleocapsid. In a recent study it was found that the molecule of tryptanthrin has antiviral potential against human coronavirus NL63. In this work, we used a combination of theoretical techniques with experimental nuclear magneti...

Artemisia species are highly important due to their economic significance as medicines, fodder and food. Artemisia cina is an endemic species to Kazakhstan. In folk medicine, water extract of A. cina was used in the treatment of bronchial asthma while the alcohol extract has larvicidal and antituberculosis activity. The most common and most extensi...

Infusions of roots of Siolmatra brasiliensis (Cogn.) Baill, (“taiuiá”, “cipó-tauá”) are used for toothache pain and ulcers. We aimed to study the antinociceptive effects and identify the possible mechanism of action of this plant and its isolated substances (cayaponoside A1, cayaponoside B4, cayaponoside D, and siolmatroside I). Hydroethanol extrac...

The in silico evaluation for the three previously synthesized compounds (Methyl (MMA), propyl (PMA) and isopropyl (IMA) N-methylanthranilate), MMA and IMA originally found in the leaf essential oil of Choisya ternata, provided a very good confirmation for the in vivo pharmacological results obtained with such compounds for a number of pharmacologic...

Crataegus almaatensis, an endemic ornamental plant in Kazakhstan is used in popular medicine due to its cardiotonic properties. The most studied species of the same genus are commonly found in Europe, which shows the importance of having the Kazakh species validated via its chemical and pharmacological studies. High-speed countercurrent chromatogra...

Phytolacca, which belongs to the family of Phytolaccaceae, are known for their use in popular medicine. Bioactivity of five extracts from Phytolacca dioica seeds were evaluated in four bioassays. A selected group of compounds from the extract that displayed the best bioactivity was analysed. The ethyl acetate extract (EAE) possessed the highest con...

The quality control is vital for the safety and efficacy of herbal medicines. However, the quality of standardization is most difficult because the studies do not cover this step. The objective of this study was quality standardization of herbal drugs of Spondias dulcis Parkinson. The leaves were dried and ground to a powder. The plant drug was eva...

The aim of this study was to investigate polyphenolic composition of different parts (leaves, flowers and fruits) of Crataegus almaatensis Pojark, an endemic plant of Kazakhstan, and compare it to a well known European Crataegus oxyacantha L. flowers. A Qual-Quant analysis was performed based on HR-MS measurements on 22 secondary metabolites: flavo...

This study presents the results of anatomic-morphological examination of vegetative (leaves) and generative (flowers, fruits) organs of the endemic plant Crataegus almaatensis Pojark – family Rosaceae, growing in the foothills of Ile Alatau mountains, Almaty region of the Republic of Kazakhstan. The plant Crataegus almaatensis Pojark was examined d...

Herba Cistanche (Cistanche species) in Traditional Chinese Medicine is used for the treatment of several diseases and symptoms, to include pain. The objective of this study was to evaluate the antinociceptive effect of the hydroethanol extract of Cistanche salsa (C.A.Mey.) Beck, Orobanchaceae, stolons in animal models of pain. Chemical composition...

In this paper, we report the first time isolation of tryptanthrin from leaves of Couroupita guianensis and its structural assignment by ¹³C NMR chemical shift calculations and experimental data. Trypyanthrin is emerging as an important new therapeutic target for the treatment of cancer, neurological disorders, and other diseases that are characteri...

Santonin, a powerful anthelmintic drug that was formely used to treat worms, is Artemisia cina's main constituent. However, due to its toxicity to humans, it is no longer in use. Kazakhstan is looking to introduce this plant as an anthelmintic drug for veterinary purposes, despite the known toxic properties of the santonin. The objective of this st...

Objectives: Tibouchina granulosa, popularly known as 'quaresmeira', belong to a genus widely used in the traditional medicine as infusions from their leaves. Other species of Tibouchina are used as antibacterial, antioxidant or antileishmanial. In this work, our objectives were to investigate the biological effects of T. granulosa in models of acu...

Choisya ternata Kunth, C. ternata var. sundance Kunth and the hybrid Choisya ‘Aztec-Pearl’ are three related species belonging to the Rutaceae family. Ethanol extracts were prepared from the leaves of these three species and evaluated in relation to their antioxidant activity using in vitro and ex vivo models. The ethanol extracts belonging to the...

Copaiba oleoresin (CPO) obtained from Copaifera landgroffii, is described as active to a large number of diseases and more recently in the endometriosis treatment. In this work poly(lactic-co-glycolic acid) (PLGA) nanoparticles containing CPO were obtained using the design of experiments (DOE) as a tool to optimize the production process. The nanop...

Choisya ‘Aztec-Pearl’, a hybrid of Choisya ternata and Choisya dumosa var. arizonica, had the antinociceptive activity in the ethanol extract (EECA) of its leaves evaluated. Two quinoline alkaloids, anhydroevoxine (A) and choisyine (C), isolated from these leaves were also tested. The results obtained pointed out to a very high antinociceptive acti...

In this paper by combining GIAO-B3PW91/cc-pVDZ//B3PW91/cc-pVDZ chemical shifts (¹H and ¹³C) calculation scaling factor protocols with NMR experimental data was supported the elucidation of the calculated NMR theoretical values obtained at GIAO-B3PW91/cc-pVDZ//B3PW91/cc-pVDZ scaling factor protocols show good correlations and high predictive power,...

In this paper by combining GIAO-B3PW91/cc-pVDZ//B3PW91/cc-pVDZ chemical shifts (¹H and ¹³C) calculation scaling factor protocols with NMR experimental data was supported the elucidation of eugenol. We present also eugenol Monte Carlo conformational analysis. The calculated NMR theoretical values obtained at GIAO-B3PW91/cc-pVDZ//B3PW91/cc-pVDZ scali...

Zanthoxylum piperitum DC. (ZP) is a traditional medicinal plant used mainly in countries from Asia such as Japan. This study aimed to investigate the antinociceptive effect of ZP essential oil (ZPEO). The major component present in the essential oil was beta-phellandrene (29.39%). Its antinociceptive activity was tested through animal models (forma...

1. Secondary compounds in nectar can function as toxic chemical defences against floral antagonists, but may also mediate plant-pollinator interactions. Despite their ecological importance, few studies have investigated patterns of spatial variation in toxic nectar compounds in plant species, and none outside their native range. 2. Grayanotoxin I...

Pereskia bleo (Kunth) DC. (Cactaceae) is a plant commonly used in popular medicine in Malaysia. In this work, we evaluate the antinociceptive effect of P. bleo leaf extracts and isolated compounds in central antinociceptive model. Ethanol extract (E), hexane (H), ethyl acetate (EA), or butanol (B) fractions (30, 50, or 100 mg/kg, p.o.), sitosterol...

Choisya ternata Kunth (Rutaceae) is native to North America where it is popularly known as "Mexican orange". In this study, the anti-inflammatory effects of the essential oil (EO) obtained from the leaves of C. ternata, one of its minor components (ternanthranin-ISOAN) and its two synthetic analogues (methyl and propyl N-methylanthranilate - MAN an...

The aim of this review was to extract information of the book Medicinal Plants in Folk Tradition: An Ethnobotany of Britain & Ireland published in 2004 by Allen and Hatfield, to give an overview of plants with medicinal potential and their applications. This study attempts to attest, observe and comment on the diversity of plants, as well as the ac...

Buddleja davidii Franch (Buddlejaceae) is traditionally and historically used for injury healing and for its anti-inflammatory and anti-bacterial effects. In the Present study the antinociceptive effects of crude ethanol extract (CEE), ethyl acetate (EA) and butanol (B) fractions obtained from the flowers were investigated, together with martynosid...

Choisya ternata Kunth (Rutaceae) is a plant species used in Mexican folk medicine for its antispasmodic and simulative properties. Recently, we identified a new alkaloid, isopropyl N-methylanthranilate, and a related one, methyl N-methylanthranilate, from the essential oil of this species and have proven them to possess antinociceptive activity eve...

The oil of babassu tree nuts (Orbignya speciosa) is a potential alternative for treatment and prophylaxis of benign prostatic hyperplasia. Improved results can be obtained by drug vectorization to the hyperplastic tissue. The main objective of this work was the preparation and characterization of poly(lactic-co-glycolic acid) (PLGA) nanoparticle an...

Ethnopharmacological relevance: Couroupita guianensis Aublet, 'macacarecuia', 'abricó-de-macaco', 'castanha-de-macaco' and 'amêndoa-dos-andes', is found in tropical regions and is widely used in the treatment of tumors, pain, and inflammatory processes. Aim of the study: Ethanol extract and hexane and ethyl acetate fractions were evaluated in mo...

Ethnopharmacological relevance: Local communities in Malaysia consume Pereskia bleo Kunth. (Cactaceae) leaves as raw vegetables or as a concoction and drink as a tea to treat diabetes, hypertension, rheumatism, cancer-related diseases, inflammation, gastric pain, ulcers, and for revitalizing the body. Aim of the study: To evaluate anti-nocicepti...

One novel compound was isolated from the hexane extract of Choisya ternata Kunth. leaves, choisyaternatine (1), together with the known compounds tecleamaniensine A (2), lup-20(29)-en-3β-ol (lupeol) (3), lup-20(29)-en-3β,24-diol (4), β-sitosterol glucoside (5), and skimmianine (6), plus the following known compounds from the dichloromethane extract...

Our aim was to identify the antinociceptive effect for P. bleo leaves as they are popularly used in Malaysia for the treatment of rheumatism, among other conditions. Plant material was collected in Malaysia (2009). An ethanol extract (E) was prepared from leaves (1.4g) and submitted to liquid liquid partition with: hexane [H], ethyl acetate [EA], a...

Babassu is the common Brazilian name of Orbignya speciosa Mart. (Arecaceae). The fruits are used for several disorders. In the present study, the antinociceptive effects of the ethanol extract (EE) and dichloromethane fraction (DF) obtained from leaves were investigated, as well as apigenin using nociception models (acetic acid-induced abdominal wr...

Leaves of Arrabidaea chica (Bonpl.) B. Verl., Bignoniaceae, are commonly used in the Amazon region as anti-inflammatory, antimicrobial, astringent and as a wound healing agent. In the Southeast Region of Brazil, this species is used for urinary infection treatment. Ethanol extract from leaves of Arrabidaea chica and its fractions were prepared and...

Anal. by GC and GC/MS of the essential oil obtained from Malaysian Curcuma mangga Val. & Zijp (Zingiberaceae) rhizomes allowed the identification of 97 constituents, comprising 89.5% of the total oil compn. The major compds. were identified as myrcene (1; 46.5%) and β-pinene (2; 14.6%). The chem. compn. of this and addnl. 13 oils obtained from sel...

2-Pentylpiperidine, named conmaculatin, a novel volatile alkaloid related to coniine was identified from the renowned toxic weed Conium maculatum L. (Apiaceae). The structure of conmaculatin was corroborated by synthesis (8 steps starting from cyclohexanol, overall yield 12%). Conmaculatin's strong peripheral and central antinociceptive activity in...

This study aimed to examine the use of natural products as medicines in the greater Dublin area, Ireland. A field study was carried out on eighteen natural health stores; store assistants were interviewed and asked questions from a previously prepared questionnaire. Results were evaluated and a picture could be drawn about the use of natural produc...

Analysis (GC and GC/MS) of an essential oil sample obtained from dry leaves of Nepeta x faassenii Bergmans ex Steam, a hybrid species produced by crossbreeding N. mussinii Spreng. with N. nepetella L., led to the identification of 109 constituents that represented 95.9% of the oil. The major constituents were 4aalpha,7alpha,7aalpha-nepetalactone (6...

Lower urinary tract symptoms (LUTS) are a common complaint among aging men and are usually caused by Benign Prostatic Hyperplasia (BPH). A number of medical treatments for LUTS/BPH exist, such as α-blockers, 5α-reductase inhibitors, phytotherapeutical drugs and combination therapies. Babassu is the common name of a Brazilian native palm tree called...

Mexican people employed infusion of leaves of Choisya ternata Kunth for their antispasmodic and "simulative properties". In the present study the detailed GC and GC-MS analyses of the essential oil of Choisya ternata Kunth (Rutaceae) were performed. The presence of a minor constituent isopropyl N-methylanthranilate (1) was revealed among other iden...

Effects of a nanocomposite containing Orbignya speciosa lipophilic extract on Benign Prostatic Hyperplasia.

Cited By (since 1996):3, Export Date: 18 October 2014

A total of twenty different extracts from six Brazilian Bromeliaceae species was screened for antioxidant activity by assessment of their capacity to scavenge the DPPH radical. In a general way, the polar rhizome extracts from Bromeliaceae representatives showed better antioxidant results than the extracts from leaves and fruits of the same species...

The antioxidant activity of leaf extracts from Jacaranda puberula Cham., Bignoniaceae, was assayed by the DPPH (2,2-diphenyl-1-picryl-hydrazyl) free radical scavenging method. Three phytomedicines (F1, F2, and F3) used as blood depurative, were tested by the same method. The free radical scavenger potential was measured by the discoloration of the...

The cell viability of Lepechinia speciosa (St. Hill) Epling fractions was measured by cell membrane integrity (lactate dehydrogenase assay) on rat basophilic leukemia cells (RBL-2H3). All fractions and extract tested (100 μg/ml) increased the release of lactate dehydrogenase (LDH), being the ethyl acetate and dichloromethane fractions with LDH rele...

Plants of the genus Bauhinia are used in several countries worldwide for the treatment of diabetes, and several related species have been shown to have hypoglycaemic effects in vivo in both normoglycaemic and alloxan- and streptozotocin-treated animal models. In this study, the insulin-secreting cell line INS-1 was used to examine the effects of th...

Verbascoside has been isolated form L. speciosa after several different chromatographic methods. After its purification, the structure has been unequivocally established using modern spectroscopic techniques. As for the antiviral activity, the maximum non toxic concentration has been established and this concentration has been used in the anti herp...

The inhibitory activity against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in vitro, at noncytotoxic concentrations of Urera baccifera (L.) Gaudich. extracts have been evaluated. As for the antiviral activity, the maximum non toxic concentration has been established and this concentration has been used in the anti herpes assay, in vitro...

The increasing interest in biodegradable nanoparticles containing biomaterials such as poly(D,L-lactide-co-glycolide) (PLGA) and chitosan for drug delivery raises issues regarding the blood compatibility of these nanoparticles, since some nanoparticles, including carbon nanoparticles, can affect human platelet aggregation and cause vascular thrombo...

Couroupitaguianensis Aubl. (Lecythidaceae) is popularly known in Brazil as "abricó-de-macaco". Infusions or teas obtained from its leaves, flowers, and barks are used in South America for the treatment of several disorders such as pain and inflammatory processes. Evaluate antinociceptive effects of crude ethanol extract (CEE) and its fractions in t...

Isoquercitrin isolated from the aerial parts of Hyptis fasciculata was evaluated according to its capacity to interfere with glioblastoma (Gbm) cell growth. Gbm cells were incubated with isoquercitrin, quercetin, or rutin at concentrations of 25, 50, and 100 mumol/l for 24, 48, and 72 h. Quercetin and rutin affected Gbm cell proliferation after tre...

Pterocaulon alopecuroides (Lamark) De Candolle and Bidens segetum Mart. ex Colla are two species belonging to the Asteraceae family. Extracts from those two species were evaluated to their cytotoxic, antioxidant and antiviral activities. All the extracts assayed have shown a very high cytotoxity against RBL-2H3 cell line. The antioxidant assay poin...