Fabienne Guérard

Fabienne Guérard
Université de Bretagne Occidentale | UBO · Laboratoire des Sciences de l’Environnement Marin - LEMAR (UMR 6539 CNRS/UBO/IRD/IFREMER)

PhD

About

130
Publications
46,608
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
2,473
Citations
Introduction
Prof. Fabienne GUERARD is Deputy Director of the Marine Environmental Sciences Laboratory UMR6539 (joint Research Unit of UBO, CNRS, IRD and Ifremer) regarding (i) enzyme engineering, (ii) valorization of marine biomass and (iii) role of enzymatic systems (phenol oxidase, antioxidant enzymes, ...) in the immune response of marine bivalves. Co-author of 75 papers and book chapters, and 134 communications. Responsible for a Master’s degree in biotechnological exploitation of marine ressources.
Additional affiliations
September 2008 - present
Université de Bretagne Occidentale
Position
  • Managing Director
October 1984 - February 1987
Collège de France
Position
  • PhD Student

Publications

Publications (130)
Article
Full-text available
Five native Sargassaceae species from Brittany (France) living in rockpools were surveyed over time to investigate photoprotective strategies according to their tidal position. We gave evidences for the existence of a species distribution between pools along the shore, with the most dense and smallest individuals in the highest pools. Pigment conte...
Article
Full-text available
Osteoporosis is an aging-related disease and a worldwide health issue. Current therapeutics have failed to reduce the prevalence of osteoporosis in the human population, thus the discovery of compounds with bone anabolic properties that could be the basis of next generation drugs is a priority. Marine plants contain a wide range of bioactive compou...
Article
Full-text available
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Article
Full-text available
Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomi...
Article
Full-text available
Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthi...
Chapter
Marine seaweeds produce protective and defensive compounds, for example, carbohydrates and phenolic compounds that are a source of bioinspiration for molecules with cosmetic and human health applications. This chapter provides a general description of seaweeds, together with a brief overview of the adaptive and chemical defenses developed by these...
Poster
Full-text available
First adhesion stages of oyster Crassostera gigas larvae are very poorly documented. Oyster larvae start their life by a two-weeks swimming phase, leading to the pediveliger stage which is characterized by the apparition of the foot, a specific organ which is essential for adhesion. Pediveliger larvae stop swimming, fall to the bottom and explore t...
Article
Full-text available
Marine macroalgae represent a valuable natural resource for bioactive phytochemicals with promising applications in therapeutics, although they remain largely under-exploited. In this work, the potential of two marine green macroalgae (Cladophora rupestris and Codium fragile) as a source of bioactive phenolic compounds was explored, and antioxidant...
Article
Full-text available
The present study focused on a brown macroalga (Halidrys siliquosa), with a particular emphasis on polyphenols and their associated biological activities. Two fractions were obtained by liquid/liquid purification from a crude hydroethanolic extract: (i) an ethyl acetate fraction and (ii) an aqueous fraction. Total phenolic contents and antioxidant...
Patent
Full-text available
The invention relates to a composition made of a collagen juice or a dry collagen extract isolated from fish skin and containing α, β, and γ chains.
Technical Report
Full-text available
Marine (= blue) biotechnology, i.e. the utilisation of marine bioresources as targets or sources of biotechnology applications, is a field with massive potential for innovation and economic growth. In the context of rapid climate change and increasing pressure on natural resources, recent advances in methodology and technology, particularly in biop...
Patent
Collagène natif de peau de poisson comprenant des chaînes α, β et γ.
Conference Paper
Full-text available
Since late 1980’s, manila clams (Ruditapes philippinarum) have been affected by the Brown Ring Disease (BRD) caused by Vibrio tapetis. Although several studies have investigated the effects of environmental parameters such as salinity, temperature, and sediment on this host-pathogen relationship, the effect of food quality has never been investigat...
Article
The smear ripened soft cheeses are characterized by a surface orange-red-brown color which has a microbial origin. For a long time this coloration was mainly imputed to Brevibacterium linens. However the latest published works, based on molecular biology, have shown a minor role for this bacterium. The present study shows the results obtained with...
Article
Full-text available
Vibrio tapetis causes the brown ring disease in the Japanese clam Ruditapes philippinarum while Vibrio aestuarianus is associated with massive oyster mortalities. As extracellular proteins are often associated with the virulence of pathogenic bacteria, we undertook a proteomic approach to characterize the secretomes of both vibrios. The extracellul...
Poster
Full-text available
Spartina alterniflora, an invasive halophyte growing in temperate salt marshes, develops effective adaptative responses, such as chemical adaptation, face to abiotic stresses, thus explaining in part its invasive nature. On another hand, biotechnological researches are currently focused on natural compounds showing promising bioactivities for speci...
Chapter
Full-text available
Phlorotannins are metabolites synthesised by brown algae to protect against environmental stresses. Only a few studies presented structural elucidation of native molecules extracted from macroalgae, contrary to previous published studies, which characterised acetylated phlorotannins. The present work introduces quantitative and qualitative studies...
Conference Paper
Full-text available
Biotechnological researches are nowadays focused on natural compounds showing promising bioactivities for specific therapeutic application and/or as surfactant on biomaterials in order to improve their biocompatibility and efficacy. Among natural compounds, phytochemicals have been found to present antioxidant and antibacterial capacities but also...
Article
Full-text available
Maillard Reaction (MR) was performed in ‘dry’ conditions at 50 °C for 48 h between native shrimp hydrolysate (HN) and xylose or dextran. Resulting emulsifying and antioxidant properties were assessed. HN was compared with a native sodium caseinate (CN) and its glycoconjugates, obtained in the same conditions. Compared to dextran, xylose resulted in...
Article
Maillard Reaction (MR) was performed in ‘dry’ conditions at 50 °C for 48 h between native shrimp hydrolysate (HN) and xylose or dextran. Resulting emulsifying and antioxidant properties were assessed. HN was compared with a native sodium caseinate (CN) and its glycoconjugates, obtained in the same conditions. Compared to dextran, xylose resulted in...
Poster
Full-text available
Marine algae have been identified as an under-exploited resource, although they have long been recognized as valuable sources of diverse bioactive compounds. These phytochemicals have been found to present antioxidant capacities but also to show effects in osteoblast proliferation and differentiation. Extracts from two green algae, Cladophora rupes...
Poster
Full-text available
Seaweeds and halophytes have been identified as an under-exploited resource, although they have long been recognized as valuable sources of diverse bioactive compounds. Moreover, several phytochemicals have been recently found to present antioxidant capacities and to stimulate bone formation. Bone diseases, in particular osteoporosis, are considere...
Article
Full-text available
Sargassum muticum, an invasive brown macroalga presently distributed along European Atlantic coasts from southern Portugal to the south coast of Norway, was studied on a large geographical scale for its production of phenolic compounds with potential industrial applications and their chemical and biological activities. S. muticum can produce high b...
Conference Paper
Full-text available
Seaweeds and halophytes have been identified as an under-exploited resource, although they have long been recognized as valuable sources of diverse bioactive compounds. Moreover, several phytochemicals have been recently found to present antioxidant capacities and to stimulate bone formation. Bone diseases, in particular osteoporosis, are considere...
Poster
Full-text available
Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally challenged with two Vibrio tapetis strains, V. tapetis strain CECT4600 the causative agent of Brown Ring Disease (BRD) and V. tapetis strain LP2 supposed to be non-pathogenic to V. philippinarum. Changes in phenoloxidase (PO) and superoxide dismutase (SOD), two major...
Poster
Full-text available
Manila clams, Venerupis philippinarum (Adams and Reeve, 1850), were experimentally infected with two different bacterial strains at two different temperatures. Bacterial strains used in this study were Vibrio tapetis strain CECT4600, the causative agent of Brown Ring Disease (BRD) and V. tapetis strain LP2, supposed non pathogenic to V. philippinar...
Data
Full-text available
Antiproliferative activity of 18 fish protein hydrolysates was measured on 2 human breast cancer cell lines grown in vitro. Three blue whiting, three cod, three plaice and one salmon hydrolysates were identified as significant growth inhibitors on the two cancer cell lines. Preliminary analysis of hydrolysates composition evidenced they contained a...
Poster
Full-text available
In temperate and tropical salt marshes, herbaceous halophytic plants develop adaptative responses face to interactive abiotic stresses, such as high UV solar radiations, salinity and temperature. The induction of an oxidative stress will generate highly toxic reactive oxygen species (ROS). Polyphenols, which are secondary metabolites involved in an...
Conference Paper
Full-text available
Along Brittany coasts, lots of populations of marine macroalgae develop in interdial zones. This environment undergoes abiotic and biotic stresses such as UV solar radiations, variations of temperatures and salinity, hydric deficiency and/or grazing which may trigger oxidative stress generating reactive oxygen species (ROS) formation at molecular s...
Data
Full-text available
Atlantic regions in Europe, such as those from Portugal, Spain, France, Ireland and United Kingdom, present economy sectors highly dependent on marine resources exploitation. The economic importance of these regions is based in marine related companies, such as ship builders, transport by ship, fishing vessels, fishing industry and fish markets. Th...
Conference Paper
Full-text available
Le milieu estuarien est particulièrement impacté par les activités anthropiques. Les xénobiotiques présents dans les eaux sont multiples : métaux, pesticides, HAPs (Hydrocarbures Aromatiques Polycycliques), PCBs (Polychlorobiphényls) … A ces stress chimiques peuvent s’ajouter les impacts du réchauffement climatique et de l’hypoxie. L’analyse de bio...
Article
Full-text available
Increasing the value of marine coproducts and territories : territorial issues The research question reviews the potential biotechnological ways for adding value to marine wastes, under the constraint of dependency for processing plants regarding the dependency to local territory. The first results of a survey conducted in Northern Brittany allow u...
Data
La pêche et la transformation des produits de la pêche génèrent une grande quantité de sous-produits, réelles sources de matières premières pour la production de substances destinées à l'alimentation humaine et animale, la cosmétique et la santé… Ce projet vise à promouvoir une utilisation rationnelle des sous-produits dans les quatre régions du Gr...
Article
The emulsifying properties of native shrimp hydrolysate (SH) and its sugar-conjugate (GSH) were studied at two concentrations (0.5% (w/w) or 1.5% (w/w)). GSH was obtained through the Maillard reaction in mild conditions (xylose; 65 °C; pH = 6.5; 4 h). Size exclusion chromatography of GSH demonstrated the occurrence of new fractions with higher mole...
Article
Full-text available
Background: Numerous studies have demonstrated that in vitro controlled enzymatic hydrolysis of fish and shellfish proteins leads to bioactive peptides. Ultrafiltration (UF) and/or nanofiltration (NF) can be used to refine hydrolysates and also to fractionate them in order to obtain a peptide population enriched in selected sizes. This study was d...
Article
Full-text available
In a global context of marine biological resource overexploitation, a better upgrading of fish and shellfish biomass is a challenge for the 21 st century. One of the main and promising issues will be the production of marine bio-ingredients using enzymatic hydrolysis. This paper presents the key steps in the production of enzymatic hydrolysates, su...
Article
Full-text available
The production by enzymatic treatment of fish protein hydrolysates (FPH) is a promising route to add value to fisheries proteinic co-products (fish frames, heads etc.). Indeed, FPH possess good nutritional properties and biological activities for food and feed uses. Pressure-driven membrane separations such as ultrafiltration (UF) and nanofiltratio...
Article
Full-text available
The objective of this study was to produce, by an enzymatic hydrolysis process at a pilot scale, a saithe (Pollachius virens) hydrolysate with a high antioxidant activity. Design of experiment methodology, based on laboratory-scale experiments, was used to obtain a behavioral reduced model that allows one to determine the optimal operating conditio...
Article
Les ressources vivantes de l'océan sont immenses et diverses. D'elles dépendent nos avenirs climatique, alimentaire, énergétique... à condition de se donner les moyens de les identifier, de les exploiter mais aussi de les gérer. Tels sont aujourd'hui les enjeux des biotechnologies marines.
Article
Ultrafiltration membranes can be advantageously used to improve the bioactivity of a saithe protein hydrolysate containing peptides having a size lower than 7kDa, by fractionating or concentrating some specific molecular weight peptide classes. The aim of this study is to evaluate the behaviour of a 4kDa membrane in modified polyethersulfone in con...
Article
Five organic tubular nanofiltration membranes of various material and molecular weight cut-off (MWCO) were tested for the enrichment of a fish (saithe, Pollachius virens) hydrolysate in low molecular weight antioxidant peptides (<2 kDa). The effect of membrane material and MWCO on permeation flux, hydrolysate adsorption extent, membrane cleaning an...
Article
In the present comparative study, we investigated the relative reactivity of three aldopentoses (ribose, xylose, and arabinose) and two hexoses (glucose and fructose) in the Maillard reaction (55°C, pH 6.5) with a hydrolysate produced on an industrial scale from marine by-products.Results revealed the prevailing propensity of pentoses over hexoses...