Fabienne Delporte

Walloon Agricultural Research Centre CRA-W · Bioengineering Unit

To evaluate the rooting ability and growth performance in semihard wood cuttings of Argania spinosa under nonmist greenhouse conditions, our experimentation was conducted with three cutting diameters (0.10.3, 0.30.6 and 0.60.9 cm), four leaf retention treatments (leafless, 2, 4 and 8 leaves) and three different rooting substrates (fine sand, peat m...

Abstract: Argania spinosa (L.) is an endemic tree species of south­western Morocco; it plays a very important socio­economic and environmental role. However, the vegetative propagation of the argan tree by traditional cuttings is limited by the difficulty of rooting and survival during transplantation in the field. Considering these facts, this stu...

Argania spinosa (L.) is an endemic tree species of south-western Morocco; it plays a very important socio-economic and environmental role. However, the vegetative propagation of the argan tree by traditional cuttings is limited by the difficulty of rooting and survival during transplantation in the field. Considering these facts, this study intende...

Soil is probably one of the most complex environments on the Earth including a huge microbial diversity. Microorganisms are largely involved in agricultural ecosystem functions. These are determined by soil type and properties (pH, C content, texture…) and environmental conditions (temperature, humidity…). The cropping system used over time is also...

Regenerative property and transgenesis competency of plant cell : physiological, histological and molecular approaches in wheat (Triticum aestivum L.) Thesis Gembloux, Belgium University of Liege – Gembloux Agro-Bio Tech - 143 p., 5 tabl., 27 fig. Summary: Widely used in plant biotechnology for over fifty years, the property of cell totipotency is...

Cherry is a member of the Rosaceae family, subfamily Prunoideae, subgenus Cerasus. It is the common name of several Prunus species such as P. avium, P. cerasus, P. mahaleb, P. serotina, P. serrulata, P. incisa and many interspecific hybrids (P. canescens x P. incisa, P. avium x P. cerasus, P. incisa x serrula, etc.).

Three durum wheat (Triticum durum Desf.) genotypes with three levels of drought tolerance were screened in order to evaluate their response to water stress at callus induction and plant regeneration levels. Significant differences were observed among the genotypes, and polyethylene glycol (PEG) levels used, and their interactions were however, sign...

Somatic embryogenesis is a powerful tool for clonal propagation and plant breeding in many plant species, but its application is limited by the genotype response based on the interaction between gene expression and culture conditions. In this context, the recalcitrance of the nonembryogenic cherry rootstock Prunus cerasus cultivar CAB 6P was invest...

Acclimatization to stress is associated with profound changes in proteome composition. The use of plant cell and tissue culture offers a means to investigate the physiological and biochemical processes involved in the adaptation to osmotic stress. We employed a new proteomic approach to further understand the response of calli to dehydration induce...

Humic substances (HS) are complex and heterogeneous compounds of humified organic matter resulting from the chemical and microbiological decomposition of organic residues. HS have a positive effect on plant growth and development by improving soil structure and fertility. They have long been recognized as plant growth-promoting substances, particul...

Cellular totipotency is one of the basic principles of plant biotechnology. Currently, the success of the procedure used to produce transgenic plants is directly proportional to the successful insertion of foreign DNA into the genome of suitable target tissue/cells that are able to regenerate plants. The mature embryo (ME) is increasingly recognize...

The physiological, biochemical and molecular mechanisms regulating the initiation of a regenerative pathway remain partially unknown. Efforts to identify the biological features that confer transformation ability, or the tendency of some cells to induce transgene silencing, would help to improve plant genetic engineering. The objective of our study...

A procedure for inducing somatic embryogenesis from leaflets has been developed with Prunus incisa, using picloram enrichment of MS basal medium combined to darkness exposure as culture treatments and 0.4 μM BAP (6- benzylaminopurine) and 0.05 μM NAA (a-naphthalene acetic acid) with daylight exposure for expression. In this study, we show that embr...

Somatic embryogenesis is a useful tool of plant breeding. In this context, a procedure for inducing somatic embryogenesis in Prunus incisa leaf explants had been previously developed. The original in vitro protocol relies on picloram treatments and exposure to darkness as inductive conditions, the best frequency of embryogenesis being obtained on t...

From a holistic perspective, the discovery of cellular plasticity, a very interesting property of totipotency, underlies many topical issues in biology with important medical applications, while transgenesis is a core research tool in biology. Partially known, some basic mechanisms involved in the regenerative property of cells and in their recepti...

Microprojectile- or Agrobacterium-mediated DNA delivery into calluses initiated from immature embryos has proven to be effective in transforming wheat. Yet, obtaining a large number of high quality immature embryos throughout the year is a laborious and delicate process. To circumvent these limitations, we propose an alternative technique applying...

A wheat regeneration system was developed using mature embryos. Embryos were removed from surface-sterilised mature caryopses (winter wheat Odeon cultivar and spring wheat Minaret cultivar) and ground to pieces through a sterile nylon mesh. The fragments were characterised by means of the image analysis technique. They were 500 M mean diameter and...

The rheological properties of wheat grains are associated with the composition of the starchy endosperm in high molecular weight (HMW) glutenin proteins. The HMW glutenin 1xDy12 subunit gene was co introduced with the screenable bar and the reporter gus marker genes in the commercial spring wheat Minaret cultivar (cv). The gene of interest and the...

Strategies for genetic transformation have been developed using organogenesis methods adapted to Inmil®(GM 9) and Damil®(GM 61/1) dwarfing rootstocks and to sweet cherry 'Summit'. Meristems from in vitro shoots were bombarded with plasmid carrying bar and gus genes, and subsequently formed shoots. A. rhizogenes-mediated transformation with bar gene...

Overexpression of the budding yeast RAS2 gene in Nicotiana plumbaginifolia cells revealed that RAS2 acted as 'suicide' gene in freshly isolated protoplasts from leaves and blocked cell proliferation in cell suspension-derived protoplasts. Among a series of genes tested (such as npt II, CDC35, PDE2), RAS2 was the only one to block the expression of...